Labshake search
Citations for Qiagen :
1 - 50 of 10000+ citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... purified with the Large-Construction kit (Qiagen) and linearized by AscI digestion ...
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bioRxiv - Microbiology 2023Quote: ... Two kit controls (Qiagen glass filter and PALL ...
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bioRxiv - Genetics 2019Quote: ... hybrid DNA was extracted using a DNeasy® kit (Qiagen). DNA concentrations were measured using a QubitTM 2.0 fluorometer (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was extracted from hybrid ESCs and NPCs using the miRNEasy Kit (Qiagen) and DNaseI treatment (Qiagen) ...
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bioRxiv - Genetics 2024Quote: ... and the hybrids was isolated from the muscle tissue using DNeasy Blood & Tissue Kits (Qiagen). The quality of DNA was checked by a NanoDrop® ND-1000 spectrophotometer with 260/280 and 260/230 ratios ...
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bioRxiv - Microbiology 2022Quote: ... LF/LF = 5 mice from two cages over two experiments) using the DNeasy PowerSoil Kit (Qiagen, Germantown, MD). Modifications to the standard protocol included a 10-minute incubation at 65°C immediately following the addition of the lysis buffer and the use of a bead mill homogenizer at 4.5 m/s for 1 min ...
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bioRxiv - Immunology 2024Quote: ... Two PCRs were performed with the PyroMark PCR kit (Qiagen) in order to report the average methylation of 8 representative CpG sites in the regulatory T cell–specific demethylated region (TSDR ...
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bioRxiv - Microbiology 2021Quote: The QIAseq SARS-CoV-2 Primer Panel paired with the QIAseq FX DNA Library construction kits (Qiagen GmbH, Germany) were used for enriching and sequencing the entire SARS-CoV-2 viral genome ...
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bioRxiv - Evolutionary Biology 2021Quote: ... fragment sizes of 200-400 bp and 400-600 bp were isolated for library construction using a MinElute Gel Extraction kit (Qiagen). We quantified DNA concentration in a Qubit2.0 ...
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bioRxiv - Developmental Biology 2019Quote: ... The two supernatants were combined and purified with MinElute kit (Qiagen) in 22µl of EB buffer ...
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bioRxiv - Zoology 2019Quote: ... We used two separate extraction kits during the study: the Gentra PureGene Tissue Kit (Qiagen) and the GeneJET Genomic DNA Purification Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... The total cell RNA of two control samples and two treated samples were extracted with RNeasy Kit (Qiagen, Cat No. 74106), and then processed and sequenced by BGI Genomics (BGI Genomics ...
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bioRxiv - Genomics 2022Quote: ... we extracted RNA from freshly dissected tissue of two male and two female singing mice (liver and brain) using a Qiagen RNeasy Mini kit (Qiagen 74104). We then homogenized the tissue using a rotor-stator homogenizer with disposable tips and did on-column DNAse digestion following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... As a first step in DNA library construction we extracted total DNA from each specimen using a Qiagen DNeasy Blood & Tissue Kit (Qiagen, Canada). The quality and quantity of each DNA sample was measured using a Nanodrop 2000 ...
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bioRxiv - Molecular Biology 2021Quote: ... photo-activated using two light sources (Halogen lamp described above or BLU-V System – Qiagen) and stored at −80°C until DNA extraction with a magnetic-based method (MagMAX CORE extraction kit – ThermoFisher).
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bioRxiv - Molecular Biology 2021Quote: Total RNA was purified from cells using the Trizol/RNeasy hybrid protocol (Trizol, Life Technologies; RNeasy Mini Kit, Qiagen). Polyadenylated [poly(A)] RNA was then purified with the NEBNext Poly(A ...
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bioRxiv - Evolutionary Biology 2020Quote: ... n=4 females [hybrid cross] and n=5 females [conspecific cross]) using the AllPrep RNA/DNA Mini Kit (Qiagen), and was stored at −80°C until sequencing ...
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bioRxiv - Microbiology 2020Quote: ... Two methods of extraction were used: the DNeasy Blood and Tissue Kit (Qiagen) and the DNeasy PowerSoil Kit (Qiagen) ...
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bioRxiv - Neuroscience 2021Quote: ... and two rounds of washes using the RNAeasy Mini Kit (Qiagen, Cat# 74106). Total RNA concentration was determined with the Nanodrop 1000 (v3.8.1 ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA was extracted from the red blood cells of the parents and 99 F2 hybrid females using the DNeasy Blood & Tissue Kit (Qiagen). The library was constructed according to a previously described method [27] ...
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bioRxiv - Evolutionary Biology 2021Quote: To evaluate genome-wide divergence among the populations used in our transcriptome sampling and to estimate the influence of hybrid ancestry on gene expression patterns we obtained genomic DNA from 270 trees using DNeasy 96 Plant Kit (Qiagen). These 270 trees included the 30 maternal trees represented in our mixed-sib family design along with previously identified 154 pure P ...
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bioRxiv - Microbiology 2021Quote: ... the presence of both parental antibiotic resistance markers was validated by PCR on DNA extracted from hybrid clones (DNeasy blood and Tissue Kit, Qiagen), targeting the antibiotic resistance genes (see Sup ...
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bioRxiv - Developmental Biology 2021Quote: ... hybrid embryos and sorted cells were prepared using QIAseq miRNA Library Prep with single index or UDI kit (Qiagen, 331502) and sequenced on HiSeq 65bp single-end sequencing or NovaSeq 50bp paired-end sequencing platform ...
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bioRxiv - Bioengineering 2023Quote: ... The bands representing the protein hybrid libraries were excised and DNA was purified using the QIAquick Gel Extraction Kit (Qiagen). The DNA concentration was then measured with the Quant-iT dsDNA (HS ...
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bioRxiv - Genomics 2019Quote: ... RNA from two brains were extracted using the RNeasy Mini Kit (Qiagen, Valencia, CA) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Two µg of each DNA sample was bisulfite modified using EpiTect Bisulfite kit (Qiagen). The Pparγ1sv promoter region was amplified with EpiTaq HS polymerase (Takara ...
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bioRxiv - Microbiology 2021Quote: ... The stage two PCR products were purified with the MinElute PCR purification kit (Qiagen) following manufacturer protocols ...
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bioRxiv - Cell Biology 2023Quote: ... Two on-column (Qiagen) and one in-solution (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... Two on-column (Qiagen) and one in-solution DNase (NEB ...
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bioRxiv - Developmental Biology 2023Quote: DNA from two separate stage 42 embryos of F0 Cab and F0 Kaga and one stage 42 embryo of F1 hybrid Kaga/Cab was extracted using DNeasy Blood and Tissue Kit (Qiagen #69504) following the manufacturer’s guidelines ...
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bioRxiv - Genomics 2023Quote: ... tetraphylla and putative hybrid breeding lines in Hawai‘i with RNA from leaf tissue extracted using RNAeasy Plant Mini Kit (Qiagen, Valencia, California). RNA was then sent to Novogene Bioinformatics Technology Co. ...
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bioRxiv - Genomics 2019Quote: ... total RNA was harvested and purified from two biological replicates using an RNeasy Kit (Qiagen) with gDNA eliminator columns ...
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bioRxiv - Neuroscience 2020Quote: ... Genomic DNA from two pooled explants was purified by using DNeasy Blood & Tissue Kit (Qiagen) for each sample ...
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bioRxiv - Plant Biology 2024Quote: RNA was extracted form two-week-old wild-type tissue using an RNeasy kit (Qiagen) and treated with Turbo DNase (Ambion) ...
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bioRxiv - Microbiology 2019Quote: ... Two volumes of RNAprotect (Qiagen) was added to each sample and the samples were incubated for 10 minutes at 25 °C ...
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bioRxiv - Microbiology 2020Quote: ... Two volumes of RNAprotect (Qiagen) was added to the cultures ...
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bioRxiv - Molecular Biology 2022Quote: ... Two volumes RNA protect (Qiagen) was added to the culture prior to RNA extraction using Monarch total RNA miniprep kit (NEB) ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA was extracted from the two fractions with the RNAeasy Mini Extraction kit (Qiagen, 74044, UK), converted in cDNA via the SuperScript™ III Reverse Transcriptase (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... Two μg of DNA was sodium bisulphite modified using the Eptech Kit (Qiagen GmbH, Hilden, Germany) in accordance with the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... DNA was extracted from leaves of one to two plants using a Qiagen MiniPrep Kit (Qiagen). Genotyping of all lines was performed using a custom lentil exome capture assay as described in Ogutcen et al ...
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bioRxiv - Physiology 2020Quote: ... DNA libraries were subjected to two rounds of bisulfite modification with the EpiTect Bisulfite Kit (Qiagen) following by 12-cycles of PCR amplification ...
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bioRxiv - Microbiology 2021Quote: ... Two DNA extractions were completed for each soil sample using the DNeasy PowerSoil kit from Qiagen (at the time PowerSoil DNA Isolation Kit by MO BIO) ...
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bioRxiv - Developmental Biology 2020Quote: ... The samples were then subjected to two consecutive bisulfite conversions using EpiTect Bisulfite Kit (QIAGEN, 59104) and PCR using PfuTurbo Cx hotstart DNA polymerase (Agilent Technologies ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA from two independent biological replicates was extracted using a commercial kit (Qiagen cat. 69504) with an optional RNase A treatment step ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from leaves of two-week-old wheat seedlings using RNease kit (Qiagen) with on-column DNase I digestion (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted from two Callogorgia delta colonies using an RNeasy extraction kit (Qiagen, Hilden, Germany). The RNA extracts were enriched for bacteria by depleting coral host rRNA using a Ribo-Zero Gold Yeast kit (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... for Illumina compatible next generation sequencing (NGS) library construction with GeneRead DNAseq Targeted Panels V2 (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: DNA from parental and all second-generation hybrid offspring (KonPar: N = 263; PruKoh: N = 193; CerEuk: N = 230) was isolated using the DNeasy Blood & Tissue Kit (Qiagen, Valencia, CA, USA) and sequenced following the Genotype-By-Sequencing (GBS ...
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bioRxiv - Genomics 2023Quote: ... The bacteriomes of two individuals were dissected and total DNA was extracted using a commercial extraction kit (DNeasy Blood & Tissue Kit, Qiagen, Hilden, Germany), following the manufacturer’s instructions for purification of total DNA from animal tissues ...
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bioRxiv - Plant Biology 2020Quote: Total RNAs were extracted from two-week Arabidopsis seedlings following The RNeasy Plant Mini Kit protocol (QIAGEN), and 5μg total RNA was converted into cDNA with Oligo(dT)20 by SuperScript™ III First-Strand Synthesis System (Invitrogen) ...