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Citations for Qiagen :
1501 - 1550 of 2105 citations for Potassium Bromate 90 95% Chemical Purity 18O3 98% 100 Ug Ml In 18O Water since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... rotated at 4°C for 1.5-2 hr in 0.75 mL equilibrated Ni-NTA Agarose (Qiagen), followed by packing pre-equilibrated Poly-Prep Chromatography Columns (Bio-Rad) ...
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bioRxiv - Systems Biology 2023Quote: Small cfRNAs were extracted from ~1 mL of plasma using the miRNeasy Serum/Plasma Kit (Qiagen). 1ul ExiSEQ NGS Spike-in (Qiagen ...
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Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... Cell pellets were resuspended in 1 mL buffer RLT from the RNeasy mini kit (Qiagen, Germany) with a 1:200 dilution of β-mercaptoethanol (Sigma Aldrich ...
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bioRxiv - Evolutionary Biology 2023Quote: ... snap frozen in liquid nitrogen and homogenized in 9.2 ml buffer G2 (Qiagen Midi Prep Kit). The samples were then transferred to a 15 ml tube and processed with a Qiagen Midi Prep Kit (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... The cell lysate was clarified by centrifugation and incubated with 1 mL Ni-NTA resin (Qiagen). The precursor protein was purified using the standard protocol from Qiagen for purifying protein from Ni-NTA resin under denaturing conditions with urea ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were loaded onto a 1 mL Ni-NTA Superflow column (Qiagen, Valencia, CA, USA) that had been equilibrated with 10 column volumes of wash 1 buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant proteins were purified on Ni-NTA columns (1 ml Ni-NTA Agarose; Qiagen Cat # 30210), washed with 20 ml hexokinase buffer (20 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... The cells sediment was added to 2 mL Red Blood Cells Lysis Solution (Qiagen, Duesseldorf, Germany) at room temperature for 5 min to remove red blood cells ...
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bioRxiv - Systems Biology 2023Quote: ... About 1 OD/mL cells were mixed with 2 volumes of RNAprotect Bacteria Reagent (Qiagen, 76506), and the cell pellet was collected by centrifuging at 2500 g for 8 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was extracted from 1 ml of overnight culture (DNeasy Blood and Tissue Kit—Qiagen) pre-treated with 100 μg of lysostaphin (Sigma cat ...
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bioRxiv - Microbiology 2023Quote: ... These were harvested by centrifugation and resuspended in 1 mL of RNA Protect Bacteria Reagent (Qiagen) with 30µL of 20 mg/mL lysozyme ...
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bioRxiv - Genomics 2023Quote: ... cfDNA was isolated from 1 mL of plasma using the QIAGEN Circulating Nucleic Acids Kit (QIAGEN), eluted in AE buffer ...
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bioRxiv - Microbiology 2023Quote: ... and the supernatant was added to 3 ml of Ni-nitrilotriacetic acid (NTA) superflow resin (Qiagen) and the mixture was applied onto a gravity drip column ...
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bioRxiv - Cancer Biology 2024Quote: ... Bulk tumor-bearing tissues were homogenized in 6 ml of cell lysis buffer (Puregene, 158063, Qiagen) using a FastPrep-24 5G tissue homogenizer (116005500 ...
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bioRxiv - Cancer Biology 2023Quote: ... the wash was repeated by using 0.7 mL PE buffer (From QIAGEN Plasmid Plus Midi Kit). After washing ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were eluted in 40 ml of elution buffer (Qiagen, 10 mM Tris-Cl, pH 8.5) with optional 15 mins of incubation at 37°C to recover long molecules ...
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bioRxiv - Immunology 2024Quote: ... 1 mL of PBS-AB was added to each tissue with Tungsten carbide 3mm beads (Qiagen). Samples were homogenized for 15 min and then centrifuged at 15,000 g for 10 min at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was transferred to a fresh tube and 5 ml of Ni-NTA resin (Qiagen) was added.
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bioRxiv - Evolutionary Biology 2020Quote: ... Between 100 mg and 200 mg of young leaves were ground in liquid nitrogen in a Qiagen Retsch Tissue Lyser (Qiagen N. V., Hilden, Germany) during 2×1 min at 30 Hz ...
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bioRxiv - Genomics 2020Quote: DNA was extracted from fecal samples and swabs using the Qiagen Powersoil® DNA Isolation Kit (Cat#12888-100) (QIAGEN Pty Ltd., Victoria, Australia) or the QIAamp Fast DNA Stool Mini Kit with MoBio bead shearing (Mo Bio ...
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bioRxiv - Neuroscience 2021Quote: ... We took each tissue and minced it with ∼100 cuts of a scalpel and proceeded to extract genomic DNA according to manufacturer’s protocol (Qiagen QIAamp DNA micro kit, 56304). The genomic DNA was then processed for sgRNA amplification and sequencing as outline in the sgRNA PCR amplification and sequencing section.
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bioRxiv - Genomics 2021Quote: ... DNA extractions were performed using a modified version of the DNeasy PowerWater Kit® (QIAGEN, Cat. No.: 14900-50-NF or 14900-100-NF) protocol that utilizes enzymatic ...
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bioRxiv - Plant Biology 2023Quote: DNA was extracted from the Poa population panel using approximately 100 mg of lyophilized leaf tissue and a DNeasy® Plant Kit (Qiagen Inc., Germantown, MD). High throughput Illumina Nextera ® libraries were constructed and samples were sequenced with other plant samples in pools of 96 individuals in one lane of an S4 flowcell in an Illumina Novaseq 6000 System with paired-end 150-bp reads ...
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bioRxiv - Microbiology 2023Quote: ... 3–20 μl of individual PCR products (adjusted on the basis of estimated relative amplicon concentration) were combined into 100 μl subpools and purified using an UltraClean 96 PCR cleanup kit (Qiagen Beverly LLC #12596-4). Despite not producing visible PCR bands ...
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bioRxiv - Genetics 2020Quote: ... The eluates were mixed with guanidine-HCl to a final concentration of 6M and incubated with Ni-NTAsepharose (Qiagen, 100 μl of slurry per sample) o/n at 4°C ...
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bioRxiv - Neuroscience 2023Quote: DNA isolation and quantification was performed as previously described43,44,68 using a high molecular weight genomic DNA purification kit according to the manufacturer’s protocol (QIAGEN Genomic tip either 20/G or 100/G) and Quant-iT Picogreen dsDNA quantification ...
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bioRxiv - Genetics 2021Quote: ... DNA was extracted from 50 ml LB from an overnight culture using a plasmid midi kit (Qiagen). Successful genomic DNA extraction was confirmed by PCR with primers gp41 inner F (5’-CAAGAGCAAAGAACCGACG-3’ ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was extracted from 1 ml of overnight culture using a QIAamp DNA Mini Kit (Qiagen). Illumina MiSeq sequencing operated by the Functional Genomics Centre Zurich and Novogene (Cambridge ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was conducted using specific 10x QuantiTect primers diluted in 1.1 mL TE pH 8.0 (Qiagen). The genes that were analyzed were Adcy1 (QT00386421) ...
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bioRxiv - Systems Biology 2020Quote: ... Cell pellets were transferred to 2 ml polypropylene tubes and disrupted twice using a TissueLyser II (Qiagen), adding the same volume of acid-washed glass beads (Sigma-Aldrich ...
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bioRxiv - Genomics 2019Quote: ... Beads were washed 4 times with 1 ml of IP buffer and 700 μl of Qiazol (Qiagen) was directly added to the beads ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 3 mL were collected and used for plasmid extraction using a QI-Aprep Spin Miniprep kit (QIAGEN). Extracted plasmids were eluted in 30 μL of elution buffer and stored at −20 °C until use.
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bioRxiv - Cell Biology 2021Quote: ... SF9 cells at 1×106 SF9 cells/ml were grown in 24 well round bottom plates (Qiagen) and infected with baculovirus of the indicated single subunits ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids were isolated from 5 mL of overnight liquid culture using QIAprep Spin Miniprep Kit (Qiagen, Germany) and the presence of mutations was confirmed by Sanger Sequencing.
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was loaded onto a gravity column containing 5 ml of 50% Ni-NTA resin (Qiagen) pre-equilibrated with 40 ml lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were then vortexed briefly and 1 mL of ice-cold QIAzol Lysis Reagent (Qiagen, cat. 79306) was added to each sample ...
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bioRxiv - Microbiology 2021Quote: ... Five hundred microliters of exponentially growing cells were stabilized in 1 mL of RNAprotect Bacteria Reagent (Qiagen). After treatment of the culture pellet with lysozyme (Qiagen) ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5 mL TRIzol added and homogenization was performed by bead beating using a TissueLyser II (Qiagen, Germany) with 0.5 mm glass beads (Scietific Industries Inc. ...
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bioRxiv - Microbiology 2019Quote: Bacterial DNA was isolated from 10 mL of urine using the QIAamp Viral RNA Mini Kit (Qiagen) according to manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... Cell lysates were clarified using ultracentrifugation and loaded on a 15 ml Ni-NTA Superflow column (QIAGEN) and washed with 7 column volumes of 20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Genomics 2022Quote: ... The samples were cooled to room temperature and 30 μL of 10 mg/mL RNase A (QIAGEN) was added and the samples were incubated at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... and passed through 1 mL of glutathione–Sepharose 4B and then onto a Ni-NTA column (QIAGEN) to capture the residual GST- and His-tag fragments ...
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bioRxiv - Systems Biology 2022Quote: ... at which point 650 μl of the culture was mixed with 1.3 mL RNAProtect Bacterial Reagent (Qiagen) and frozen according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was purified using either 10 mL nickel-chelating column or Strep-Tactin column (Qiagen). The N-terminal His-SUMO tags were cleaved from the eluted protein by TEV protease overnight and removed by passing through a second nickel column ...
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bioRxiv - Cell Biology 2022Quote: ... Condensin I was bound to Glutathione Sepharose 4B beads (Cytiva) in a 5 mL polypropylene column (Qiagen) using GST- tagged mSMC4 and eluted by PreScission Protease (Cytiva ...
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bioRxiv - Neuroscience 2021Quote: ... RNAsin Plus 400 units/ml (Fischer Scientific PRN2615) followed by resuspension in Qiagen RLT buffer (Qiagen 79216) with 1% 2-mercaptoethanol (Sigma Aldrich M6250).
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bioRxiv - Molecular Biology 2020Quote: ... Cell debris was removed by centrifugation and supernatant was mixed with 1 ml Ni-NTA Agarose (Qiagen) and incubated for 90 minutes at 4 °C with gentle shaking ...
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bioRxiv - Microbiology 2021Quote: ... Frozen lung sections were homogenized in 1 ml of Lysis/Binding solution in a TissueLyser II (Qiagen) with three cycles of at 30 Hz for 30 seconds ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were cooled to room temperature and treated with 2.5 µl of RNAse A (10mg/mL, Qiagen) at 37°C for 2 hours ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was mixed with 1 ml of 50% Ni2+-NTA His-bind resin (Qiagen, Hilden, Germany), incubated for 1 hr at 4°C with mild rotatory shaking ...