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Citations for Qiagen :
1701 - 1750 of 2214 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Total genomic bacterial DNA extraction was performed from frozen fecal samples using the Qiagen DNeasy PowerSoil HTP Kit (Cat. No. 12955-4, Qiagen, Valencia, CA, USA). Adequate DNA yield was confirmed using NanoDrop spectrophotometry ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from the hippocampus and cortical regions of Ctrl WT and cKO mice (n=4 per group) using the RNeasy kit (Qiagen, Valencia, CA, USA) method (see location of cortical areas 1 and 2 in the Supplementary Figure 1) ...
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bioRxiv - Microbiology 2022Quote: ... exposed to 0.4 nM and 4 nM of β-estradiol for 10 days) Vagina Chips using QIAzol lysis reagent (Qiagen, cat. no. 79306). Complimentary DNA was synthesized using a SuperScript VILO MasterMix (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... The soluble fraction was separated from debris by centrifugation (9000 g, 4°C) and incubated for 60 minutes with Ni-NTA beads (Qiagen, cat. no. 30230) pre-equilibrated in binding buffer ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted from dolphin oral samples and 32 negative (PBS) controls using the DNeasy UltraClean 96 Microbial Kit (Qiagen Cat. #10196-4) following manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... for 30 min at 4 °C before being applied to a 10mL bed volume Nickel nitrilotriacetic acid (Ni-NTA) agarose (Qiagen, cat. no. 30230) column ...
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bioRxiv - Zoology 2021Quote: ... PCR amplifications were carried out in 10 μl reaction volumes containing 5 μl Type-it Microsatellite PCR Kit (Qiagen, Hilden, Germany), 0.5 μM of each primer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Multiplex amplification was carried out in 10 μl reaction volumes containing 5 μl of QIAGEN Multiplex PCR Buffer Mix (QIAGEN Inc.), 0.2 μM labeled forward primer (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... flies were individually crushed in 400 µL of lysis buffer by a sterile 5-mm stainless bead shacked by a TissueLyser® (Qiagen) for 30 s at 25 Hz ...
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bioRxiv - Neuroscience 2020Quote: ... RAGE-/- and SOD1G93A x RAGE-/- mice (n = 5 - 6) using RNeasy Lipid Tissue extraction kit according to manufacturer’s instructions (QIAGEN, CA, USA). The total RNA was purified from genomic DNA contamination using Turbo DNase treatment (Ambion ...
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bioRxiv - Molecular Biology 2021Quote: Cells were seeded in 6-well and 12-well plates at a density of 1.25×105-5×105 cells per well and transfected with miR-320a mimic (Qiagen, 339173 YM00471432) or antagomir (GenePharma ...
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bioRxiv - Immunology 2021Quote: ... Single cells were sorted into individual wells of a 96-well plate containing 5 μl of lysis buffer (TCL buffer (Qiagen #1031576) with 1% of 2-b-mercaptoethanol) ...
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bioRxiv - Bioengineering 2021Quote: ... The 319 bp DNA size of PCR products were clarified by 1% agarose gel electrophoresis using 5 μL PCR products and remained DNA were purified by QIAquick PCR Purification Kit (Qiagen, 28104). The purified PCR products were sequenced by Sanger Sequencing approach (GeneWiz ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were collected by centrifugation (5 min at 500 x g) and RNA was isolated using RNeasy Mini Kit (RNeasy Mini Kit, Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... and the tissue was homogenized with a 5 mm tungsten carbide bead in a bead mill homogenizer (Qiagen, Tissue Lyser II) at 30 Hz for 1.5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... and the sample was divided into 5 parts and each part was purified using one PCR purification columns (PCR purification kit, Qiagen 28106). 50ul elution buffer was used the elute each column ...
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bioRxiv - Microbiology 2022Quote: ... and the amplified DNA bands from the 5’ and 3’ ends were individually excised and purified with QIAquick® Gel Extraction Kit (QIAGEN). Purified PCR products were cloned into pJET1.2/blunt plasmid (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were centrifuged for 5 minutes at 300g and resuspended in 1mL PBS + RNase Inhibitors (Invitrogen AM2694, Qiagen Beverly Inc Y9240L) and fixed as previously described for split-pool ligation-based RNAseq ...
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bioRxiv - Biochemistry 2020Quote: ... embryo pellets were homogenized by cryo-milling after addition of a 2.5 or 5 mm steel bead using a tissue lyser (QIAgen TissueLyser II) at 25 Hz for 15 seconds 3-6 times ...
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bioRxiv - Genomics 2022Quote: ... Cells were maintained at 37°C in 5% CO2 for 3 days before collecting genomic DNA using DNeasy Blood & Tissue Kits (Qiagen, 69504) and sequencing.
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bioRxiv - Genetics 2022Quote: ... 0.08M KOH was added to each well and incubated at room temperature (RT) for 5 min and then washed with EB (Qiagen, 19086). Next ...
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bioRxiv - Genomics 2022Quote: ... The swabs were placed in Red 1.5 mL RINO® screw-cap tubes (NextAdvance) pre-filled with RNase-free zirconium oxide beads and QIAzol Lysis Reagent (Qiagen) was added ...
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bioRxiv - Microbiology 2021Quote: ... 5) 240 µL of the supernatant is subjected to viral RNA extraction using the QIAamp Viral RNA Mini Kit (Qiagen, #52904), according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... suspended in 1 mL of (v/v) 50% ACN and homogenized in a bead mill (50 Hz, 5 min; TissueLyser LT, Qiagen, Germany) using two 5 mm tungsten balls ...
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bioRxiv - Genomics 2021Quote: ... We checked ASOs penetration in cells by means of the 5′-FAM-labeled control ASO A provided by Qiagen (Figure S5f).
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bioRxiv - Neuroscience 2021Quote: ... 5 ng of each PCR product was pooled into one tube for purification using the QIAquick PCR purification kit (Qiagen #28106) and eluted into 30 µL Nuclease Free Water ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 SCID mT3-2D subcutaneous tumors and the mT3-2D cell line using a DNeasy Blood and Tissue Kit (Qiagen, #69504). Indexed ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from the colon tissue of mice (n=5) by using the RNeasy mini kit (Cat# 74104, Qiagen, Germany) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Single CD3-CD8-CD14- CD16- CD20+CD38+ BG505 SOSIP+YU2-gp140+ B cells were sorted into individual wells of a 96-well plates containing 5 μl of a lysis buffer (Qiagen, 1031576) per well using a FACS Aria III (Becton Dickinson) ...
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bioRxiv - Developmental Biology 2020Quote: ... the beads were transferred to a new 0.5ml tube and incubated in 100 μL ChIP elution buffer (10mM Tris-HCl pH8.0, 5mM EDTA, 300mM NaCl, 0.5% SDS) containing 5 µL proteinase K (Qiagen, 20mg/ml stock) at 55°C for 2 h and then at 65°C for 4 h ...
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bioRxiv - Immunology 2022Quote: ... following the final boost (Week 18 or 23) were sorted into individual wells of a 96-well plate containing 5 μL of lysis buffer (TCL buffer (Qiagen, 1031576) with 1% of 2-β-mercaptoethanol) ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... for 3 h at 37°C under 5% CO2 and total cellular RNA was purified using PureLink™ RNA kit (Life Technology) or RNeasy Mini Kit (Qiagen) and the concentration and RNA Integrity Number (RIN ...
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bioRxiv - Plant Biology 2023Quote: PCR primers 515/806 with barcoding on the forward primer were used in a 28 cycle PCR (5 cycle used on PCR products) using the HotStar Taq Plus Master Mix Kit (Qiagen, USA) to target the 16S rRNA gene for region V3 and V4 ...
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bioRxiv - Cell Biology 2023Quote: ... Spheroids were transferred into PCR-clean 1.5 mL microtubes with 100 µL of lysis buffer (LB1 from Macherey-Nagel or 700 µL of Buffer RLT (79216, Qiagen). Lysates were snap-frozen and stored at −80 °C ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5 mL of the culture was immediately treated with a double volume (10 mL) of RNA protect bacteria reagent (Qiagen, Germany) for 5 minutes at room temperature ...
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bioRxiv - Genomics 2023Quote: The bacterial 16S rRNA V4 variable region was amplified for 5 cycles with the HotStarTaq Plus Mastermix Kit (Qiagen, Hilden, Germany). Samples were purified with Ampure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... and 10 zirconium beads were added to approximately 5 mg of intestinal tissue stored at -80 °C in RNA later tissue reagent (Qiagen, Germany). A Tissue lyser (Qiagen ...
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bioRxiv - Systems Biology 2024Quote: ... The samples were then cooled for 5 minutes on ice and transferred to a pre-spun 15 mL MaXtract tube (Qiagen 129065) containing an equal volume of chloroform to the acid phenol used ...
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bioRxiv - Molecular Biology 2023Quote: Total DNA was extracted from mouse parotid gland tissue (n=5/group) following the manufacturer’s protocol for DNeasy Blood & Tissue Kit (Ref. No. 69504, Qiagen, Hilden, Germany). Mitochondrial DNA (mtDNA ...
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bioRxiv - Developmental Biology 2022Quote: ... The resulting 1.5 L bacterial culture was incubated at 37°C for 2h and a Maxiprep performed using Qiagen MaxiPlus kit (Qiagen #12963) to a resulting 1 mg of plasmid DNA ...
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bioRxiv - Genomics 2022Quote: ... Individual nuclei were sorted on a Sony Sorter SH800 with a 100 μm sorting chip into wells of a 96-well plate containing 5 μl of Buffer TCL (Qiagen #1031576) with 1% β-mercaptoethanol (ThermoFisher #21985023).
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bioRxiv - Microbiology 2022Quote: ... The cells were then centrifuged at 300g for 5 minutes and lysed using buffer RLT provided in the RNAeasy Plus Micro Kit (Qiagen; 74034). After the lysate was homogenized using the QIAshredder Kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... 5% CO2 for 72 hours before lysis and RNA extraction using the RNeasy Micro Plus extraction kit (Qiagen, Cat. No. 74034). A further technical replicate was resuspended in RPMI supplemented with 20% FCS ...
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bioRxiv - Microbiology 2022Quote: ... The spooled DNA was washed with approximately 5 mL of 70% ethanol and dried before being suspended in 300µL of EB buffer (QIAGEN, Hilden, Germany). Purified DNA was quantified by Qubit (Thermofisher).
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bioRxiv - Cancer Biology 2023Quote: Ctnnb1 exon 3 skipping was confirmed in several tumour samples by extracting total RNA from 5-20 mg of tissue using the RNeasy Plus Universal minikit (Qiagen, #73404). cDNA was synthesized using the RevertAid protocol (ThermoScientific ...
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bioRxiv - Microbiology 2023Quote: ... 20-40 mg of tissue was placed in a tube containing 600 μl of OptiMEM and a 5 mm stainless steel bead (Qiagen; #69989) and homogenized in the Qiagen TissueLyser II by two dissociation cycles (1,800 oscillations/minute for 2 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... Colony PCR was performed with KOD One™ PCR Master Mix (Toyobo) to screen colonies before recovering plasmids with a 5 mL overnight culture and miniprep (QIAgen).
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bioRxiv - Cancer Biology 2024Quote: ... additional DNA from 1-5 mL of plasma was extracted using the QIAamp® Circulating Nucleic Acid Kit (Qiagen, catalogue # 55114). Illumina sequencing libraries were constructed using SRSLY® PicoPlus DNA NGS Library Preparation Kit (ClaretBio – Cat ...
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bioRxiv - Evolutionary Biology 2024Quote: Total RNA was extracted from 5- or 7-day post-fertilization (dpf) stage animals using RNeasy Mini Kit (Qiagen, cat. 74104) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Hand SFs were transfected with 50 nM antisense LNA targeting HOXD10 (Qiagen, Sequence: 5′-TGT CTG CGC TAG GTG G-3′), HOXD11 (Qiagen ...