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Citations for Qiagen :
1901 - 1950 of 2214 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: The supernatant was carefully removed to a 50-mL sterile tube and mixed with 2 mL volume of 50% Ni-NTA resin (Qiagen) which was pre-equilibrated in the buffer A ...
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bioRxiv - Neuroscience 2021Quote: ... PCR products were run on a 2% agarose gel and gel bands were manually extracted and purified using QIAquick PCR & Gel Cleanup Kit (QIAGEN) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted from SK-N-AS and SK-N-BE(2) xenograft tissue using the RNeasy Mini Kit (Qiagen) and quality control was performed with Agilent Tapestation according to the manufacturer’s instructions ...
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Oligomerization state of the functional bacterial twin arginine translocation (Tat) receptor complexbioRxiv - Biophysics 2021Quote: ... The supernatant was loaded onto a 10 x 1 cm column with 2 mL Ni-NTA Superflow resin (Cat. #30230, Qiagen) that was pre-equilibrated with Buffer A (10 mM CAPS ...
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bioRxiv - Cancer Biology 2020Quote: ... Second PCR products were pooled and gel purified from a 2% agarose gel using the MinElute Gel Extraction kit (QIAGEN). Purified product concentration was measured using a Qubit (Thermo Scientific) ...
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bioRxiv - Plant Biology 2021Quote: RNA was extracted from root tips (1-2 mm) of 7-d-old seedlings using the RNEasy plant mini kit (QIAGEN), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: RNA was extracted from 14-day-old wild-type and acinus-2 pinin-1 seedlings using RNeasy mini kit (Qiagen) and treated with TURBO DNA-free Kit (Ambion ...
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bioRxiv - Molecular Biology 2020Quote: ... the supernatant was added to 2 ml of pre-equilibrated Nickel-nitrilotriacetic acid (Ni-NTA) agarose (QIAGEN, Cat. No. 30210) 50% slurry and rotated at 4°C for 2 h ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... total DNA from 2×106 of Burkitt cells was extracted by the Blood & Cell culture DNA mini kit (Qiagen #13362). For extracellular viral DNA extraction ...
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bioRxiv - Plant Biology 2021Quote: ... thaliana seedling material was ground in a 2 ml reaction tube with the aid of a pre-cooled tissue lyser (TissuelyserII, Qiagen). RNA from ground tissue was extracted with an RNA purification Kit (Roboklon) ...
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bioRxiv - Molecular Biology 2021Quote: ... The legs and cephalothorax were separated from flash-frozen spider specimens and then homogenized with a BioMasher II (Funakoshi) and mixed with 2 mL of Buffer G2 (QIAGEN) containing 200 µg/mL RNase A ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from ∼0.5 to 2×106 cells per sample using the RNeasy Plus Mini Kit (Qiagen, 74134) with RNase-free DNase treatment (Qiagen ...
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The spatial landscape of progression and immunoediting in primary melanoma at single cell resolutionbioRxiv - Cancer Biology 2022Quote: ... The retrieved tissue mROIs were deposited with 2 µl PBS into PCR tubes containing 18 µl of lysis buffer: 1:16 mix of Proteinase K solution (QIAGEN) in PKD buffer (QIAGEN) ...
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bioRxiv - Bioengineering 2022Quote: ... RNA was isolated from 3D samples (n=2-3) by combining a TRIzol-based cell lysis with a RNeasy Mini Kit (Qiagen). Samples were collected at aforementioned timepoints ...
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bioRxiv - Biochemistry 2022Quote: Amplification was verified by running the PCR product on a 2% agarose gel and the PCR products pooled and cleaned up with QIAquick Nucleotide Removal Kit (Qiagen). The DNA content was quantified with Quant-iT PicoGreen dsDNA Assay Kit.
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bioRxiv - Pathology 2022Quote: ... The N gene-specific primers were used to amplify 97 bp of SARS-CoV-2 N gene by conventional PCR and purified by Qiagen gel extraction kit (Qiagen ...
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bioRxiv - Genomics 2022Quote: ... The six samples of each technical replicate were pooled together equimolarly and the final product was purified from a 2% agarose gel with 20 ul silica beads (QIAEX II Gel Extraction Kit, Qiagen).
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bioRxiv - Microbiology 2022Quote: ... 2 throat swabs were collected with eNAT™ swabs for microbial DNA extraction using the PowerFecal DNA isolation kit (Qiagen), and in PBS for lactobacilli cultivation on MRS agar ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... per condition or genotype were homogenized in 2-ml Eppendorf tubes containing lysis buffer with 1% beta-mercaptoethanol using a TissueLyser LT bead mill (Qiagen) and 5-mm stainless steel beads (Qiagen #69989) ...
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bioRxiv - Developmental Biology 2020Quote: ... run on a 2% agarose gel and a band of 300bp approximately was cut and gel purified using QIAquick Gel Extraction Kit (QIAGEN). Library concentration was determined with KAPA Library Quantification Kit for Illumina Platforms (Kapa Biosystems) ...
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bioRxiv - Cancer Biology 2020Quote: Cells were seeded in 96-well plates (2 × 104 cells/well) and reverse-transfected with 100 ng/well of Cignal ISRE-Luc reporter (Qiagen) using Lipofectamine 3000 ...
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bioRxiv - Molecular Biology 2020Quote: ... run on a 2% agarose gel and a smear of 200-500bp was cut and gel purified using QIAquick Gel Extraction Kit (QIAGEN). Library concentration was determined with KAPA Library Quantification Kit for Illumina Platforms (Kapa Biosystems) ...
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bioRxiv - Molecular Biology 2020Quote: ... run on a 2% agarose gel and a smear of 200-500bp was cut and gel purified using QIAquick Gel Extraction Kit (QIAGEN). Library concentration was determined with KAPA Library Quantification Kit for Illumina Platforms (Kapa Biosystems) ...
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bioRxiv - Molecular Biology 2020Quote: ... Total RNA was extracted from individual filter sets (n = 2 per timepoint) using Qiagen RNeasy Mini Kit (Qiagen, Hilden, Germany) as in Harke et al ...
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bioRxiv - Plant Biology 2021Quote: ... were amplified using biotin-labeled primers (Supplementary Table 2) synthesized by Sangon Biotech (Shanghai, China) and purified by a PCR purification kit (Qiagen). The EMSA was conducted as described previously(An et al. ...
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bioRxiv - Microbiology 2021Quote: ... 10 µl of the cell suspension from each dilution was dispensed into 24 wells of a 96 well plate containing 2 μl One-step RT-PCR enzyme (Qiagen), 10 μl 5x One-step RT-PCR buffer (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: ... Mosquito bodies were homogenized in PBS supplemented with 20% FBS and 2% penicillin/streptomycin with 5mm stainless steel beads with a TissueLyser (Qiagen) prior to RNA isolation ...
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bioRxiv - Microbiology 2020Quote: 3-5 × 105 cells with or without treatment with IFNα and SARS-COV-2 infection were harvested and total RNA was extracted using the RNeasy kit (Qiagen) employing on-column DNase treatment ...
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bioRxiv - Microbiology 2020Quote: ... buffer with lysozyme (1mg/mL) and incubated at RT for 2 min prior to adding 700 μL RLT Buffer (Qiagen) with 10ul/mL B-mercaptoethanol (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was extracted from the testis and striatum of three 2 months old mice using the RNeasy Mini Kit (Qiagen) and was used for random-primed cDNA synthesis with SuperScript III reverse transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... GluA1 or GluA2Q (pIRES2-mCherry or pIRES2-EGFP) and CNIH2 (pRK5 or pBOS) plasmids were transfected at a 1:2 ratio using Effectene (QIAGEN) into adherent HEK293T cells (ATCC ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA concentration and purity were measured by a spectrophotometer and 2 μg were used to synthesize cDNA by QuantiTect Reverse Transcription kit (Qiagen). Real-time amplification was performed in a Mastercycler EP Realplex (Eppendorf ...
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bioRxiv - Molecular Biology 2020Quote: ... eluates were supplemented with imidazole at a final concentration of 10mM and incubated for 2 hours with 200 µL of Ni-NTA agarose beads (Qiagen) that were prewashed in buffer C (20mM HEPES [pH 7.6] ...
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bioRxiv - Immunology 2021Quote: ... 150 ng DNA per reaction was amplified in duplicate using primers and probes specific to γHV68 Orf50 and mouse Ptger2 (see table) and 2× QuantiNova Probe Mastermix (Qiagen). Standard curves were obtained by serial dilutions of Orf50 and Ptger2 gBlocks (ORF50 ...
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bioRxiv - Genetics 2021Quote: Total mRNA was isolated from 2 dpf or 7 dpf zebrafish homogenates using the RNeasy Mini Kit (Qiagen, Cat# 74104) and reverse-transcribed with SuperScript VILO (ThermoFisher ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from 2-week-old leaves by using QIAGEN RNeasy Plant Mini Kit (QIAGEN, Germantown, MD, 74904). Five μg of total RNA was treated with DNase following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Sorted CD14+ monocytes from total PBMC either ex vivo or after a 20 hour stimulation with 106 UV-inactivated SARS-CoV-2 viral particles per 106 cells were lysed with RLT Plus buffer (QIAGEN). RNA was isolated using the RNeasy Micro Plus Kit (QIAGEN ...
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bioRxiv - Synthetic Biology 2020Quote: Cells were grown in triplicates on JMM 40 mM glycine and 40 mM pyruvate till mid-log phase and 1-2 mL of cultures were harvested and stabilized directly by RNA Protect Bacteria Kit (Qiagen). Cells were then lysed using lysozyme and beating with glass beads in a Retschmill (MM200 ...
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bioRxiv - Genomics 2020Quote: ... The pooled sample was quantified using the KAPA Library Quantification Kit and adjusted to 2-4nM with Buffer EB (QIAGEN) for sequencing on Illumina platforms ...
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bioRxiv - Molecular Biology 2020Quote: ... Genomic DNA was extracted from samples of 2×109 cells after lysis using the DNeasy Blood and Tissue Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The effect of IscR-C92A on σ70-dependent activity was determined by incubating IscR-C92A with 2 nM supercoiled pPK12778 [purified with the QIAfilter Maxi kit (Qiagen)] ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: 3-5 x 105 cells infected or not with SARS-CoV-2 or HCoV-NL63 were harvested and total RNA was extracted using the RNeasy kit (Qiagen) employing on-column DNase treatment ...
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bioRxiv - Zoology 2020Quote: ... 8 μL of the amplified product were electrophoresed in an ethidium bromide stained 2% gel The amplicons obtained were isolated and purified using a QIAquick pcr purification kit (Qiagen), and the purified products were sequenced by Eurofins ...
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bioRxiv - Cell Biology 2020Quote: ... and gene expression was assessed using RT2 Profiler PCR Array Human WNT Signaling Pathway Plus (PAHS-043YC-2, Qiagen Germany). Arrays were run on QuantStudio6 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue microregions were deposited with 2 µl PBS into PCR tubes containing 18 µl of lysis buffer: 1:16 mix of Proteinase K solution (QIAGEN) in PKD buffer (QIAGEN) ...
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bioRxiv - Immunology 2021Quote: ... A total of 1-2 μg of RNA was used to synthesize the first single-strand cDNA using QuantiTect Reverse Transcription kit (Qiagen). For RT-PCR amplification ...
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bioRxiv - Genetics 2020Quote: U8 DNA templates containing a T7 consensus sequence were PCR amplified from human or zebrafish genomic DNA (see Supplementary Table 2 for primer sequences) and purified after agarose gel electrophoresis using a QIAEX II kit (Qiagen). Human and zebrafish U8 snoRNAs were generated using 400-1000ng of template DNA and a mMESSAGE mMACHINE T7 kit (Life Technologies ...