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Citations for Qiagen :
1601 - 1650 of 6611 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... samples obtained by FACS together with 100 ng carrier RNA (PolyA- and PolyA+ mixed in 98:2 ratio) were lysed by Buffer RLT Plus (Qiagen, USA). Then we used PolyA mRNA Magnetic Isolation Module (NEBNext ...
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bioRxiv - Cancer Biology 2023Quote: DNA was extracted from NCI-PC35-1 and NCI-PC35-2 organoids using an AllPrep DNA/RNA Mini Kit (Qiagen 80204) according to the manufacturer’s protocol for animal cells ...
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bioRxiv - Microbiology 2023Quote: ... The DNA fragment of interest was excised from a 2% agarose gel and purified with QIAquick Gel Extraction Kit (Qiagen Inc.). The NEBNext Ultra DNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Biophysics 2022Quote: ... A low-pressure chromatography column connected to a peristaltic pump (2 mL/min flow rate) was prepared with 15 mL nickel resin (Qiagen #30230), followed by equilibration with 50 mL Nickel A Buffer ...
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bioRxiv - Immunology 2023Quote: ... 120 mg l− 1 sodium pyruvate and 1% penicillin–streptomycin) at 300 Hz for 2 minutes using a TissueLyser II (Qiagen, Germany).
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bioRxiv - Immunology 2023Quote: ... in vitro derived Foxp3+ cells were harvested on day 2 and day 6 into Trizol and total RNA was isolated with miRNeasy Micro Kits (QIAGEN 217084) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were homogenised in QIAzol reagent (1 mL) in a 2 mL safe lock tube with stainless steel beads using a TissueLyser II homogeniser (Qiagen, UK) at 30 Hz until fully homogenised (1-2 min) ...
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bioRxiv - Cancer Biology 2023Quote: ... Deproteination of the D-2-HG quantification aliquot was achieved using 3.0 mL of Proteinase K (Qiagen, Catalog #19131, Hildren, Germany) per 100.0 mL of cell lysis solution ...
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bioRxiv - Evolutionary Biology 2023Quote: Tissue pools were homogenised for 2 min at 30 Hz using 5 mm steel beads on a TissueLyser II instrument (Qiagen, Germany). A volume of 0.2× chloroform (Carl Roth ...
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bioRxiv - Microbiology 2024Quote: Total RNA was extracted from each 2 g of bulk and rhizosphere soils of the pepper plants using a RNeasy PowerSoil Total RNA Kit (Qiagen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... The digest was incubated for 2 hours at 37°C then purified using a QiaQuick PCR purification kit (Qiagen Cat#28106). Fragments of 2,100 bp were size-selected using a SageELF instrument (Sage Science ...
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bioRxiv - Developmental Biology 2024Quote: ... and 2 replicates for differentiation days 14 and 18) were prepared and purified according to the RNeasy Mini Kit (Qiagen, 74104). Purified RNA was sent to Novogene for sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... 7.5 ml blood were collected into EDTA blood collection tubes and processed within 2 h using the AdnaTest (Qiagen, Hilden, Germany). Patients were divided into HSPC ...
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bioRxiv - Immunology 2024Quote: ... Astrocytes were isolated using the Anti-ACSA-2 MicroBead Kit (Miltenyi, #130-097-678) and RNA extracted using RNeasy Micro Kit (Qiagen, # 74004). RNA library preparation ...
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bioRxiv - Cell Biology 2024Quote: ... The cDNA thus obtained was diluted to 2 ng/µl and specific target miRNAs were amplified by qPCR using the miRCURY LNA SYBR Green PCR kit (Qiagen, 339346). Expression of all targets was normalised against the expression of two reference genes (SNORD48 and U6 ...
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bioRxiv - Microbiology 2020Quote: ... and then cell lysates were collected by washing cells twice with PBS and adding 100ul of RNA lysis buffer (Qiagen) to each well.
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested by centrifugation at 4°C followed by cell lysis/RNA extraction using RNEasy plus mini kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and CD4 memory T cells were sorted on a FACSAria III cell sorter (Becton Dickinson) into QIAzol Lysis Reagent (QIAGEN). A total of 100,000 cells was sorted per sample ...
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bioRxiv - Genetics 2021Quote: ... were processed for DNA extraction using the Genomic-tip 20/G (mini prep, starting with approx. 108 cells) or 100/G (midi prep, 109 cells) kit (QIAGEN), according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2020Quote: WGA of single cells was performed by multiple displacement amplification using the REPLI-g Single Cell kit (Qiagen, Valencia, CA) as per the manufacturer’s instructions but at one quarter of the recommended volumes ...
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bioRxiv - Molecular Biology 2021Quote: ... MDA was done directly on cells (Supplementary figure 9) using the REPLI-g Single Cell Kit from Qiagen (Cat #150343) following kit-specific protocol ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was extracted from MTX-resistant HT-29 cells and control samples using the Gentra Puregene Cell kit (Qiagen) and the library for PacBio ...
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bioRxiv - Immunology 2021Quote: RNA was extracted from the cell pellets of human primary male and female PT cells using the RNAeasy Mini Kit (Qiagen). After quantifying RNA concentration in a Nanodrop instrument (Thermo) ...
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bioRxiv - Microbiology 2021Quote: ... a volume equivalent to 5×108 cells were spun down (assuming 1 OD600=8×108 cells) and Protect Bacteria RNA Mini Kit (Qiagen) was used to extract total RNA as described in (Bhattacharyya et al. ...
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bioRxiv - Cell Biology 2021Quote: Total RNA from HGC (3 x 107 cells) and SPZEJ (3-30 x 107 cells) was extracted with the RNeasy Plus Mini Kit (Qiagen), and the purity and concentration of the extracted RNA was evaluated with the Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Neuroscience 2020Quote: DNA and RNA from FAC-sorted cells were isolated simultaneously from the same cell pellet using an AllPrep DNA/RNA Micro Kit (Qiagen) with on-column DNase treatment during the RNA isolation ...
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bioRxiv - Genetics 2021Quote: ... These single cells or nuclei were whole-genome amplified by a REPLI-g Single Cell Kit (Qiagen, Venlo, the Netherlands) for 3 hours ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA (gDNA) was extracted from the isolated cells and unsorted cells (“Input”) with QIAamp DNA Maxi kit (Qiagen, #51104).
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA from CRISPR-on HEK293T cells and control cells were purified using a AllPrep DNA/RNA/Protein Mini Kit (Qiagen). Then ...
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bioRxiv - Cell Biology 2021Quote: ... After the last irradiation cells were given 24 hours to recover and gDNA was isolated using the Blood & Cell Culture DNA Midi Kit (Qiagen) according the manufacturers protocol (DNA content of MRC-5 cells was estimated at 10 pg per cell ...
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bioRxiv - Cancer Biology 2021Quote: RNA from infected cell lysates was harvested by incubating infected Vero E6 cells for 10 minutes with RLT buffer (Qiagen) containing 2-Mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was isolated from single-cell clone or bulk GFP+ and GFP- cells (n = 3 with cells from three different mice in each group) using QIAzol lysis reagent (Qiagen). DNA was removed using the RNase-Free DNase Set (QIAGEN) ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was extracted from tissues (CC) or cells (microglial GFP+ cells sorted by FACS) using the RNeasy Mini Kit (Qiagen). After reverse transcription using Superscript First-Strand Synthesis System (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... shRNA expressing cells were isolated by fluorescence activated cell sorting at passage doubling 1 and 15 followed by genomic DNA isolation with Gentra Puregene cell kit (Qiagen). Sequencing libraries were prepared by subjecting 18 μg (1.2 μg x 15 reactions ...
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bioRxiv - Immunology 2022Quote: Clonal naïve GMP and neutrophil total RNA was extracted from cells at 5×106 cells/ml using the Qiagen RNeasy Plus Mini Kit (Qiagen) with DNaseI treatment (RNase free DNase Set ...
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bioRxiv - Immunology 2019Quote: Total RNA was extracted from sputum cell pellets or bronchial epithelial cell lysates stored in RLT buffer (RNeasy kit, Qiagen) and 2μg was used for cDNA synthesis (Omniscript RT kit) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then harvested and subjected for genomic DNA isolation using “Blood & Cell Culture DNA Mini Kit” (Qiagen, Hilden, Germany). Reactivation of LCLs was determined by real-time quantitative PCR (qPCR ...
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bioRxiv - Immunology 2019Quote: DNA and RNA were extracted from cells using either Blood & Cell Culture DNA Mini Kit and RNAeasy isolation kit (Qiagen), or all-prep DNA/RNA kit (Qiagen) ...
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bioRxiv - Immunology 2020Quote: RNA from isolated fresh B cells and B cells activated with LPS/IL-4 was extracted using a RNeasy kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted from wild-type and ΔTMPRSS2 293T cell lines using the Puregene cell kit according to the manufacturer’s instructions (Qiagen, #158388). Co-transfection of pJC144 (guide 1 ...
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bioRxiv - Microbiology 2020Quote: ... or experimental siRNA 72 h after transfection (approximate cell concentration was 1.6×105 cells/ well) were harvested and lyzed using the QIAshredder Kit (Qiagen; 79656). mRNA was harvested using the RNeasy Plus Micro Kit (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... was extracted from the top (5.61 million cells) and bottom (4.87 million cells) fractions using the QIAamp DNA mini kit (QIAGEN cat.#51304). Fixed cells were decrosslinked by overnight incubation in 200 µL of PBS with added Proteinase K and 200 µL buffer AL (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: RNAs were prepared from purified D0 cells and from D7 to D14 populations isolated by flow cytometric cell sorting using an RNeasy Plus kit (Qiagen), and then depleted of rRNAs and processed for RNA-Seq library construction using a TruSeq Stranded mRNA Library Prep Kit (Illumina) ...
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bioRxiv - Cancer Biology 2021Quote: ... gDNA was isolated from at oncogene-negative mouse cell lines or FACS-sorted Tomatopositive cancer cells using either the AllPrep DNA/RNA(Qiagen) or the DNeasy Blood and Tissue Kit ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... and CD45+ Calcein+ DAPI-CD19+ CD38+ activated B cells were single-cell sorted into 96-well plates with catching buffer (RLT lysis buffer (Qiagen) with 1% 2-mercaptoethanol (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were removed and the remaining cell pellet (500 μl) was transferred into a 15 ml tube containing 2.5 ml RNAprotect® Cell Reagent (Qiagen). The cells were resuspended by vortexing ...
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bioRxiv - Microbiology 2021Quote: Total RNA (5×106 cells) was extracted from FEA or CRFK cell lines by using the RNeasy Plus Mini Kit (Qiagen). RNA from FEA ...
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bioRxiv - Immunology 2021Quote: Human B cells harvested post gene-editing or cell sorting were lysed for RNA extraction by the RNeasy Micro Kit (74004, Qiagen). Primers used for reverse transcription and library amplification were modified from previous29 ...
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bioRxiv - Genomics 2021Quote: ... DNA was isolated from thawed APOE cell lines by Bioneer A/S using the Blood & Cell Culture DNA Mini Kit (Qiagen). For analysis of Pax8-CreERT2 insert high molecular DNA was prepared from mouse ears using MagAttract HMW DNA Kit (Qiagen ...
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from cultured in vitro or in vivo primed T cells or ex vivo sorted T cells using the RNeasy kit (Qiagen) as per the manufacturer’s instructions ...