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Citations for Qiagen :
1301 - 1350 of 2210 citations for Potassium Bromate 90 95% Chemical Purity 18O3 98% 100 Ug Ml In 18O Water since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Filtered cell lysate was applied to 10 mL of Ni-NTA resin (Qiagen) which had been previously equilibrated with 10 column volumes of H2O ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was bound to a 3X5 mL StrepTactin Superflow Plus cartridges (Qiagen) using a 1 mL/min flow rate ...
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bioRxiv - Developmental Biology 2023Quote: ... the endometrial tissue was transferred to a cryotube containing 1.5 mL RNAlater (Qiagen), vigorously shaken for a few seconds ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue was fully homogenized in 2 ml of QIAzol buffer reagent (Qiagen) on ice and Dounce homogenization ...
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bioRxiv - Molecular Biology 2024Quote: Gcn4 and Med15 supernatants were mixed with 5 ml Ni-NTA beads (Qiagen) and incubated at 4°C for 1 hour ...
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bioRxiv - Biophysics 2024Quote: ... The resulting supernatant was incubated with 1.0 mL Ni-NTA Superflow resin (Qiagen) at 4 °C for 2 hours with rotation ...
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bioRxiv - Biochemistry 2024Quote: ... the supernatant was applied to 5 ml of Ni-NTA agarose beads (Qiagen). The beads were washed with 200 ml of wash buffer (50 mM Tris-HCl ...
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bioRxiv - Synthetic Biology 2024Quote: ... and TY1C2 were mixed with 5 μg/ml of RNase A (19101, QIAGEN) and 1 mg/ml of Proteinase K (EO0491 ...
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bioRxiv - Microbiology 2021Quote: ... Parasite cultures were synchronized by repeated sorbitol treatment and 100 μg of purified plasmid DNA (Plasmid Midi Kit, Qiagen, Valencia, CA) was transfected in P ...
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bioRxiv - Molecular Biology 2021Quote: 2 x 107 cells were harvested by centrifugation and genomic DNA was prepared using Blood & Cell Culture DNA Midi Kit 100/G Genomic-tips (Qiagen #13343). To examine alkaline degradation 3 μg of DNA was treated with 0.3 M NaOH at 55 °C for 2 hr in 15 μl ...
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bioRxiv - Molecular Biology 2020Quote: ... The replicated amplicons for each sample were combined into a single reaction of 100 μl and purified using a QIAquick PCR Purification Kit (Qiagen, 28104) and quantified on the Qubit Fluorometer ...
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bioRxiv - Plant Biology 2019Quote: ... 50,000 protoplasts in a volume of 100 μL were transformed with 20 μg of each plasmid (2 μg/μL) purified using the Plasmid Maxi kit (Qiagen, Germany). One batch of protoplasts was treated with an equivalent amount of water and used as the negative (untransformed ...
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bioRxiv - Neuroscience 2019Quote: ... total RNA was extracted from 200 μl conditioned medium or 100 μl foetal plasma using the miRNeasy Mini Kit and the miRNeasy Serum/Plasma Kit (Qiagen, Germany). microRNA expression levels were analysed using the nCounter Rat v1.5 miRNA Expression Assay (NanoString Technologies ...
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bioRxiv - Genomics 2019Quote: ... To each sample 100 μl PBS and 250 μl Ethanol was added and RNA was purified using the Qiagen RNeasy mini kit (Qiagen 74104). RNA was next treated with DNaseI (NEB ...
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bioRxiv - Genetics 2019Quote: ... 50 µM LNA oligo in 100 µL serum free media is mixed with 12 µL HiPerfect transfection reagent (Qiagen, Valencia, CA) and incubated for 20 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... DNA corresponding to the size of mononucleosomes (100-200 bp) was re-isolated from a 3% agarose gel using MinElute Gel Extraction Kit (Qiagen, # 28604). As input ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed twice in PBS and incubated for 20 min at 54 °C (60 min at 52 °C for tissue sections) in mercury locked-nucleid acid (LNA) miRNA ISH Buffer 80 nM hybridiziation buffer (100 nM for tissue section) (Qiagen, 339450). Digoxigenin labeled miRCURY LNA probes (HSA-MIR-10B-5P ...
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bioRxiv - Systems Biology 2021Quote: Genomic DNA for sequencing was isolated with the the Qiagen 100/G kit according to the manufacturer’s description (Qiagen, Hilden, Germany) and library preparation and sequencing was done as described previously using Illumina Miseq sequencing (Illumina ...
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bioRxiv - Immunology 2021Quote: ... a new empty 200 µL filtered tip was inserted to top channel outlet and 100 ul RNase easy lysis buffer (Qiagen, #74034) was used to lyse epithelial cells by quickly pressing and releasing the micropipette plunger 3 times ...
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bioRxiv - Microbiology 2019Quote: ... 1 µM of forward and reverse oligos were heated for one minute at 100°C with 5 mM MgCl2 and 7 mM Tris-Cl (i.e. Qiagen Elution Buffer) and annealed by slowly cooling to room temperature.
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bioRxiv - Microbiology 2022Quote: ... An aliquot of EDTA-treated whole blood (100 μl) was diluted with 600 μl of AVL inactivation buffer (Qiagen, Hilden, Germany), and RNA was extracted using a Viral RNA mini-kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... metagenomic DNA was extracted from a soil sample taken from the Northwestern University campus (coordinates 42.05662, −87.674247) using a DNeasy PowerSoil Kit (Qiagen, cat#12888-100). Quantification of DNA for all experiments was performed using a QuantiFluor ONE dsDNA system (Promega ...
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bioRxiv - Physiology 2021Quote: Approximately 100 ng/µL of total DNA was extracted from 20 mg tissue (QiAMP DNA mini kit Qiagen, Valencia, CA, USA). DNA from bone marrow was extracted from the bone marrow suspension and centrifuged for 10 min at 2000 g ...
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bioRxiv - Plant Biology 2021Quote: ... and salt treated samples (100 mM, 250 mM and 500 mM NaCl) was extracted using RNeasy Plant Mini Kit (Qiagen, Germany). RNA quality and quantity assessment ...
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bioRxiv - Immunology 2019Quote: Total RNA yields from the PBMC and stimulated PBMCs were in the range of 3 micrograms (50-100 ng/μl in 30μl Qiagen EB buffer) determined by absorbance at 260nm on the NanoDropTM One (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... The replicated amplicons for each sample were combined into a single reaction of 100 ul and purified using a QIAquick PCR Purification Kit (Qiagen, 28104) and quantified on the Qubit Fluorometer ...
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bioRxiv - Genomics 2020Quote: ... Dehydrated samples were stored at −80 °C until resuspension in 25 μl or 100 μl of Buffer EB (QIAGEN, Venlo, Netherlands).
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bioRxiv - Plant Biology 2020Quote: ... For expression analysis total RNA was extracted from 100 mg of freeze-dried leaf tissue of chilli and tomato lines using RNeasy plant kit (Qiagen, USA) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Frozen muscle was pulverized in liquid nitrogen and genomic DNA was extracted using a Genomic Tip 100/G kit (Qiagen, USA). DNA quantity was measured using a NanoDrop ND-8000 spectrophotometer and a Qubit dsDNA BR Assay kit (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... DNA synthesis reaction was carried out in 100 μl using 10 pmol of each primer with HotStarTaq Plus DNA Polymerase (Qiagen, 203603). Purified dsDNA products were amplified with 10 pmol of each primer in a 100 μl total reaction volume ...
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bioRxiv - Genetics 2022Quote: ... Size selection was also used for purification of 100 bp-200 bp PCR products by QIAquick Gel Extracton (28706, QIAGEN, Germany).
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bioRxiv - Genomics 2022Quote: ... Individual nuclei were sorted on a Sony Sorter SH800 with a 100 μm sorting chip into wells of a 96-well plate containing 5 μl of Buffer TCL (Qiagen #1031576) with 1% β-mercaptoethanol (ThermoFisher #21985023).
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bioRxiv - Genetics 2022Quote: ... The aqueous phase was mixed with an equal volume of 100% ethanol and transferred to an RNeasy MinElute spin column (Qiagen, 74104) for purification according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellet was rinsed with 70% ethanol and dried at room temperature before dissolving in 100 μL of RNase-free DNase set (Qiagen 79254) and incubated at room temperature for 10 minutes to remove DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... total RNA was extracted from a pool of 100–120 adult females per line using the RNeasy plus mini kit (Qiagen, Belgium) with 4 replicates for strains MAR-ABi and JP-RRi and 5 replicates for strains ROS-ITi and SOL-BEi ...
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bioRxiv - Genomics 2023Quote: ... The sample was then centrifuged at 15000 rpm for 10 min and the pellet was resuspended in 100 μl buffer EB (Qiagen, 19086). For each sample of a 96-well plate ...
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bioRxiv - Molecular Biology 2023Quote: ... the other half of tumor tissues were lysed using lysis buffer (0.1% Triton X-100) and a TissueLyser homogenizer (Qiagen, Hilden, Germany). DNA for PCR was isolated from 50 µl of tissue lysate using the Maxwell RSC tissue DNA Kit (Promega ...
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bioRxiv - Microbiology 2023Quote: ... DNA extraction was performed from 200 mg of three independent samples using the MO BIO’s Powersoil DNA isolation kit procedure (Qiagen 12888-100) as described previously (von Gastrow et al. ...
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bioRxiv - Bioengineering 2023Quote: ... samples were suspended in RNeasy kit Lysis Buffer and β-mercaptoethanol (1:100 v/v) and homogenized using QIAshredder columns (Qiagen). Samples loaded on spin columns were treated with DNase (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: MiR libraries were generated from 100 -150 ng of cell using the QIAseq miRNA Library Kit according to the protocol (Qiagen, Germany). Library quality control and concentrations were determined using a High Sensitivity D1000 ScreenTape with TapeStation Analysis Software A.02.02 (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial genomic DNA was isolated from pure culture using QIAGEN Genomic-tip 100/G following the manufacturer’s instruction of (Qiagen, Valencia, CA). The Seqwell plexWell LP384 Library Preparation Kit and Native Barcoding Kit 24 V14 (SQK-NBD112.24 ...
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bioRxiv - Developmental Biology 2024Quote: Bisulfite conversion was performed on 100 ng of genomic DNA using the EpiTect Fast DNA Bisulfite kit from Qiagen (Cat.# 59824) and DNA libraries were constructed using the NUGEN Ovation RRBS Methyl-seq System (Cat.# 0353 ...
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bioRxiv - Cell Biology 2024Quote: Cell pellets or lung lobes from individual mice were snap frozen in 350µl of 1:100 β-mercaptoethanol in the RLT Lysis Buffer from the RNAeasy mini plus Kit (Qiagen, 74134). Cell samples were thawed and vortexed while lung samples were homogenized for total RNA extraction intended for qPCR or bulk RNA-seq ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted using a modified form of the QIAGEN DNeasy PowerWater kit (QIAGEN, Hilden, DEU, cat. no. 14900-100-NF) method ...
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bioRxiv - Cell Biology 2024Quote: ... all samples were diluted to the same RNA concentration and 250 ng of RNA from each sample spiked with 100 ng mouse RNA to control reaction efficiency were reverse transcribed using the miScript II RT kit (Qiagen, 218161) with HiFlex Buffer according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... ∼200 picked cells were stored in RLT buffer (Cat. No. 79216 – 220 mL, Qiagen) with 1% 2-Mercaptoethanol (M6250-100ML ...
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bioRxiv - Molecular Biology 2021Quote: ... 300 mM of NaCl and 2 μL of RNase A (0.5 mg / mL) (Qiagen) were added to the collective eluates which were incubated at 65 °C overnight ...
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bioRxiv - Immunology 2021Quote: ... Periplasmic fraction was incubated with 1 mL pre-washed Ni-NTA agarose (30230, Qiagen) for 1 h at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... the supernatant was applied to 2 mL of pre-washed Ni-NTA resin (Qiagen) at 4°C ...
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bioRxiv - Genomics 2020Quote: ... purified nucleic acids were treated with 1-5 μl 1 mg/ml RNaseA (Qiagen) for 45 minutes at 37 °C to degrade RNA ...