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Citations for Qiagen :
1501 - 1550 of 6677 citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: DNA was extracted from sorted cells using a Gentra Puregene Cell kit (Qiagen, catalog no. 158767). Immunoglobulin heavy-chain family–specific PCRs were performed on genomic DNA samples using primers in FR1 and JH as described previously (41 ...
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bioRxiv - Immunology 2021Quote: The sorted cells were washed thoroughly and the cell pellet was suspended in RLT Buffer (Qiagen) and stored at -800C until RNA extraction ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed with PBS and then cells were lysed in 350 μL RLT buffer (Qiagen). Total RNA was extracted through columns using RNeasy kit (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... Total RNA from A549 cells (1 × 106 cells) was extracted using miRNeasy Mini kit (Qiagen, Denmark) according to the manufacturer’s instructions and eluted in 60 µl of nuclease free water ...
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bioRxiv - Genetics 2019Quote: ... RNA from collected cell types and (cancer) cell lines was isolated using the miRNeasy kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was isolated from FACS-sorted cells using Qiagen RNA Mini (>100,000 cells, Qiagen 74104), RNA Micro (<100,000 cells ...
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bioRxiv - Cancer Biology 2023Quote: ... cell lines or mammary gland epithelial cells were extracted using RNeasy Plus Mini Kit (Qiagen, #74136). Extracts were then treated with RNase free DNase (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA of 107 cells was purified using the Blood & Cell Culture DNA Midi Kit (Qiagen) following the supplier’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The cells sediment was added to 2 mL Red Blood Cells Lysis Solution (Qiagen, Duesseldorf, Germany) at room temperature for 5 min to remove red blood cells ...
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bioRxiv - Bioengineering 2023Quote: ... The cell lysates were then obtained by adding 350 µL of cell lysis buffer (Qiagen, Germany) to the centrifuged cells ...
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bioRxiv - Microbiology 2020Quote: ... Cells were lysed in FastPrep (Qiagen) for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected using Effectene (Qiagen) as per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were collected in Qiazol (Qiagen) and total RNA was extracted using a miRNeasy Mini Kit (Qiagen ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were transfected using Effectene (Qiagen) according to instructions provided by the manufacturer ...
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bioRxiv - Neuroscience 2020Quote: Cells were collected with qiazol (Qiagen) and stored at -80°C until further processing ...
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bioRxiv - Biochemistry 2021Quote: Cells were collected in QIAzol (Qiagen) and snap frozen in liquid nitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... or RNeasy Mini Kit (Qiagen; cells). cDNA was synthesized using 1 μg of mRNA using the SuperScript™ III Reverse Transcriptase (Invitrogen™ # 18080044 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were resuspended in RNAprotect (Qiagen) prior to extracting total RNA using an RNeasy minikit (QiAgen) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transfected with Effectene (Qiagen) (200ng DNA per well of a 24 well plate) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were transfected with effectene (Qiagen) according to manufacturer specification and as described in (Montigny et al. ...
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bioRxiv - Neuroscience 2021Quote: ... FastLane Cell Probe Kit (Qiagen; #216413), and ViiA7 Real Time PCR system (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected using Effectene (Qiagen), incubated for 40-48 hours at 28°C and then lysed in RIPA buffer (140mM NaCl ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with Superfect (Qiagen) or Turbofect (Thermo Scientific)-transfection reagent according to manufacturer’s instructions for 24 h and either imaged live or after fixation with 4 % PFA.
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bioRxiv - Immunology 2020Quote: ... using Gentra Puregene Cell Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected using Effectene (Qiagen) as per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were transfected using Effectene (Qiagen) and selected in Insect-Xpress medium supplemented with 5 μg/ml puromycin (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: Cells were lysed in Qiazol (Qiagen), and RNA extracted using Rneasy Mini Kit (Qiagen) ...
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bioRxiv - Cell Biology 2022Quote: ... RNA Micro (<100,000 cells, Qiagen 74004); and from the collagenase lipid layer using Qiagen RNA Lipid Tissue Mini Kit (Qiagen 74804 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected with siRNA (Qiagen) using Lipofectamine RNAiMAX transfection reagent (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: Cells were lysed using Qiazol (Qiagen) for RNA isolation ...
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bioRxiv - Genetics 2023Quote: Cells were lysed in Qiazol (QIAGEN) and total RNA was purified using the miRNEasy mini kit (QIAGEN ...
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bioRxiv - Genetics 2023Quote: Cells were lysed in Qiazol (QIAGEN) and total RNA was purified using the miRNEasy mini kit (QIAGEN ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were lysed in RLT (Qiagen) + 40 mM dithiothreitol (Sigma-Aldrich ...
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bioRxiv - Genetics 2023Quote: Cells were run through QIAshredder (Qiagen) and total RNA was extracted using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with Effectene (Qiagen) and 1ug of total plasmid (either Pactin>HPO-30:6xMyc (pXD384 ...
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bioRxiv - Microbiology 2023Quote: ... and Cell Lysis Solution (QIAGEN, Germany), and purified using GeneJET Genomic DNA Purification Kit (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were transfected using Effectene (Qiagen) in 12-well plates ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were sonicated following QIAexpressionist (Qiagen) protocols 9 and 10 using 8M UREA for protein denaturation ...
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bioRxiv - Genomics 2020Quote: ... for 4 hours to inhibit reporter transgene expression (t4) prior to isolation of DNA and RNA using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen# 80004) according to the manufacturers protocol ...
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bioRxiv - Genetics 2019Quote: ... protein altering inframe InDels (n=28) and missense variants (n=557) were uploaded to Ingenuity pathway Analysis (Qiagen, Venlo, The Netherlands). Additionally ...
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bioRxiv - Immunology 2020Quote: ... Proteins (as well as DNA and RNA) were then extracted from the lysate using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Germany) following manufacturers instructions ...
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bioRxiv - Immunology 2020Quote: ... Proteins (as well as DNA and RNA) were then extracted from the lysate using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Germany) following manufacturers instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Cancer samples were snap-frozen in liquid nitrogen and DNA extracted using the AllPrep DNA/RNA/Protein mini kit (QIAGEN, Germany) as previously reported 18 ...
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bioRxiv - Biochemistry 2020Quote: ... Canonical pathways associated with differentially expressed proteins were identified by Ingenuity Pathway Analysis (IPA, Ingenuity Systems, Qiagen, Redwood, CA, USA; www.ingenuity.com). This program is based on an algorithm that uses curated connectivity information from the literature to determine the network of interactions among the differentially expressed proteins and canonical pathways in which they are involved (Calvano et al. ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant protein in the supernatant was purified to 90% purity by a single-step chromatography on Ni++ - metal chelate affinity (Qiagen, UK) columns and eluting at 0.5 M imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Matrix crystallization screens were performed at room temperature using the sitting drop vapor diffusion method by mixing equal volumes of protein sample and reservoir using a TTP LabTech Mosquito robot and commercially-available screens (Hampton Research and Qiagen). Initial hits were optimized and crystals were obtained in 20% PEG 3350 at 20 °C ...
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bioRxiv - Neuroscience 2020Quote: ... For the UCP4 antibody protein samples were extracted from the paraformaldehyde fixed tissue using the Qproteome FFPE Tissue Kit (Qiagen, Germany). The tissue blocks analyzed here were taken from the anterior cingulate and occipital cortex (as described above ...
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bioRxiv - Immunology 2022Quote: Initial matrix crystallization trials against 576 conditions were performed at room temperature using the sitting drop vapor diffusion method by mixing equal volumes of protein sample and reservoir using a TTP LabTech Mosquito robot and commercially-available screens (Hampton Research and Qiagen). Initial hits were optimized and crystals for unliganded BG24S60A were obtained in 0.25 M potassium chloride ...
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bioRxiv - Biophysics 2020Quote: ... Bacterial extracts were prepared as described46 and the recombinant protein was purified using an Ni-NTA Superflow cartridge (Qiagen, Hilden, Germany) and eluted with imidazol47 ...
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bioRxiv - Biochemistry 2019Quote: ... Bound STAT3 proteins were then labeled with Alexa488-conjugated anti-6 × His antibodies (1 hr incubation, 1:20 dilution, Qiagen 35310). The array was washed and scanned using a GenePix 4400A scanner (Molecular Devices ...