Labshake search
Citations for Qiagen :
1401 - 1450 of 6341 citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was supplemented with 10 mM imidazole and His-tagged proteins were purified by affinity chromatography on NiNTA column (Qiagen) at room temperature ...
-
bioRxiv - Biophysics 2022Quote: ... membranes were solubilized in 1.2% N-decyl-β-maltoside (DM) and the protein was purified on a Ni-NTA affinity column (Qiagen). Following His-tag removal ...
-
bioRxiv - Systems Biology 2022Quote: The co-immunoprecipitated RNA samples were submitted to protein digestion using proteinase K (Fermentas) and purified using the MinElute Reaction Cleanup Kit (QIAGEN) with a DNase treatment step ...
-
bioRxiv - Microbiology 2023Quote: ... Cell debris was removed by centrifugation and His-tagged ClpC1 proteins were purified from supernatants with Ni-NTA agarose beads (Qiagen). After washing in binding buffer supplemented with 20 mM imidazole ...
-
bioRxiv - Bioengineering 2023Quote: ... The bands representing the protein hybrid libraries were excised and DNA was purified using the QIAquick Gel Extraction Kit (Qiagen). The DNA concentration was then measured with the Quant-iT dsDNA (HS ...
-
bioRxiv - Immunology 2023Quote: RNA was extracted from cell lysates of bovine granulocytes and sorted PBMC subsets of three to seven Holstein Friesian cattle using the AllPrep RNA/Protein kit (QIAGEN) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2022Quote: The libraries midi-preps were digested with HindIII and NheI restriction enzymes and the insert containing the mutated protein was gel purified (MinElute Gel Extraction Kit, QIAGEN) to be later cloned into the AbundancePCA plasmid and BindingPCA plasmids by temperature-cycle ligation ...
-
bioRxiv - Microbiology 2023Quote: All proteins from previously frozen samples (tumors and healthy parts of murine colon) were extracted using AllPrep DNA/RNA/Protein Mini Kit (Qiagen). Protein concentrations were estimated using NanoDrop A280 absorbance ...
-
bioRxiv - Plant Biology 2023Quote: ... This clarified extract was used for the purification of the His-tagged proteins using Ni-NTA agarose resin (Qiagen, USA). The agarose resin was washed twice by resuspending in lysis buffer followed by centrifugation at 3000 rpm and removing the supernatant ...
-
bioRxiv - Microbiology 2023Quote: ... and the purity of the purified proteins was checked by SDS-PAGE and western blot using anti-His antibody (1:1000, Qiagen) as primary antibody ...
-
bioRxiv - Microbiology 2023Quote: ... The protein was purified under denaturing conditions by nickel-nitrilotriacetic acid (NTA)-agarose affinity chromatography as described by the manufacturer (Qiagen). A polyclonal rabbit antiserum was raised by Labcorp Early Development Laboratories Inc ...
-
bioRxiv - Microbiology 2023Quote: ... The protein concentrations of all the collected samples were adjusted to 400 μg mL−1 using the Qubit protein broad range assay kits (Qiagen). The Cyclic AMP XP® kit ELISA plates were prepared according to the manufacturer’s protocols ...
-
bioRxiv - Biochemistry 2022Quote: Approximately 25 mg of frozen tissues were transferred to 2 mL Eppendorf Protein Lobind tubes containing one 5 mm stainless steel bead (cat# 69989, Qiagen) and 500 µL of lysis buffer consisting of 5% SDS in 50 mM TEAB with protease inhibitors cocktail (cat# A32953 ...
-
bioRxiv - Biophysics 2023Quote: ... coli strain M15-[pREP4] (for transformation of recombinant plasmids) and RNAse (for protein purification) were purchased from Qiagen (Valencia, CA). All other chemicals were obtained from Sigma-Aldrich (St ...
-
bioRxiv - Cell Biology 2023Quote: ... significantly regulated human proteins (t-test, p-value ≤ 0.01; described above) were characterized by core analysis in IPA® software (version 68752261, QIAGEN Inc. ...
-
bioRxiv - Biophysics 2023Quote: ... Cleaved PCNA was separated from His-SUMO and uncleaved His-SUMO-PCNA by incubating the protein solution with 5 ml Ni-NTA suspension (Qiagen) resuspended in solution D on a tilting table for 30 min ...
-
bioRxiv - Biochemistry 2023Quote: ... Tris–HCl 20 mM (pH 7.5)) by sonication and the His-tagged proteins were purified on a Ni-NTA column (Qiagen Inc.), eluted with imidazole in buffer A but complemented by ATP 1 mM + MgCl2 3 mM in the case of the Vibrio cholerae helicase ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cleaved sP36 protein was afterwards purified by a second step of affinity using Ni-NTA agarose (Qiagen, Hilden, Germany) in phosphate buffer A.
-
bioRxiv - Plant Biology 2023Quote: ... The recombinant protein was partially purified by affinity chromatography on nickel-nitrilotriacetic acid (Ni-NTA) agarose beads (Qiagen, http://www.qiagen.com). Purification steps were carried out as per the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... Proteins harvested after 6 days post transfection were purified from the supernatant on a Ni-NTA Superflow column (#30430, Qiagen). Immediately after the elution ...
-
bioRxiv - Cancer Biology 2024Quote: ... Tumour samples were fresh-frozen in liquid nitrogen and stored at -80°C for protein isolation or preserved in RNAlater solution (Qiagen) for RNA isolation ...
-
bioRxiv - Bioengineering 2024Quote: ... The output of differentially expressed proteins was further investigated using Ingenuity Pathway Analysis® (IPA; Qiagen®, Redwood City, USA). Data was also independently analysed using PEAKS studio (Bioinformatics Solutions® Inc. ...
-
bioRxiv - Biochemistry 2024Quote: ... Cells were collected by double centrifugation and 6x His-tagged target protein in the supernatant was purified using the medium by Ni-NTA (Qiagen) affinity chromatography ...
-
bioRxiv - Molecular Biology 2021Quote: RNA was harvested from cell pellets of 1 million cells using the RNeasy Mini kit (Qiagen). DNA was digested using the TURBO DNA-free Kit (Ambion ...
-
bioRxiv - Molecular Biology 2019Quote: ... Cells were trypsinized and 0.5 × 106 cells were plated directly with Effectene transfection mixture (Qiagen, 301427) on 0.1% gelatin (in-house production ...
-
bioRxiv - Neuroscience 2019Quote: Total RNA was isolated from NIH3T3 cells using the FastLane Cell One-Step Buffer Set (Qiagen). The mouse embryo brains at E10.5 were dissected in cold PBS ...
-
bioRxiv - Cancer Biology 2020Quote: Whole bone marrow cells were subjected to red blood cell (RBC) lysis (RBC lysis solution, Qiagen) and resuspended in IMDM media containing 10% FBS and 1% penicillin-streptomycin ...
-
bioRxiv - Cancer Biology 2020Quote: ... extracted cell clusters were incubated with a cell lysis mixture (20µL RLT Plus buffer (Qiagen 1053393), plus 1µL of spike-in RNA (1:250,000) ...
-
bioRxiv - Microbiology 2020Quote: ... total RNA from infected cells and uninfected cells were extracted using Qiagen RNeasy mini kit (Qiagen) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: HEK293T cells and TLR-MCV1 cells were transfected using PolyFect Transfection Reagent (Qiagen, Cat No. 301105) with 400 ng of plasmid in a 24-well plate according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2021Quote: ... we isolated DNA from single cell suspensions or single cell pellets using a DNeasy Kit (Qiagen) according to manufacturer’s protocol ...
-
bioRxiv - Microbiology 2019Quote: ... cytoplasmic and host membrane-containing cell fractions were obtained using the Qproteome Cell Compartment Kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2019Quote: ... DNA from ~7×106 cells was extracted using a Blood & Cell Culture DNA Midi Kit (QIAGEN) and amplified to examine the coverage of mutant cell libraries ...
-
bioRxiv - Immunology 2021Quote: DNA was extracted from sorted cells using a Gentra Puregene Cell kit (Qiagen, catalog no. 158767). Immunoglobulin heavy-chain family–specific PCRs were performed on genomic DNA samples using primers in FR1 and JH as described previously (41 ...
-
bioRxiv - Immunology 2021Quote: The sorted cells were washed thoroughly and the cell pellet was suspended in RLT Buffer (Qiagen) and stored at -800C until RNA extraction ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were washed with PBS and then cells were lysed in 350 μL RLT buffer (Qiagen). Total RNA was extracted through columns using RNeasy kit (Qiagen ...
-
bioRxiv - Biochemistry 2020Quote: ... Total RNA from A549 cells (1 × 106 cells) was extracted using miRNeasy Mini kit (Qiagen, Denmark) according to the manufacturer’s instructions and eluted in 60 µl of nuclease free water ...
-
bioRxiv - Genetics 2019Quote: ... RNA from collected cell types and (cancer) cell lines was isolated using the miRNeasy kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: Total RNA was isolated from FACS-sorted cells using Qiagen RNA Mini (>100,000 cells, Qiagen 74104), RNA Micro (<100,000 cells ...
-
bioRxiv - Molecular Biology 2023Quote: ... The DNA of 107 cells was purified using the Blood & Cell Culture DNA Midi Kit (Qiagen) following the supplier’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... cell lines or mammary gland epithelial cells were extracted using RNeasy Plus Mini Kit (Qiagen, #74136). Extracts were then treated with RNase free DNase (Qiagen ...
-
bioRxiv - Cell Biology 2023Quote: ... The cells sediment was added to 2 mL Red Blood Cells Lysis Solution (Qiagen, Duesseldorf, Germany) at room temperature for 5 min to remove red blood cells ...
-
bioRxiv - Bioengineering 2023Quote: ... The cell lysates were then obtained by adding 350 µL of cell lysis buffer (Qiagen, Germany) to the centrifuged cells ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were lysed in FastPrep (Qiagen) for 10 min ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were transfected using Effectene (Qiagen) as per manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2019Quote: ... cells were collected in Qiazol (Qiagen) and total RNA was extracted using a miRNeasy Mini Kit (Qiagen ...
-
bioRxiv - Developmental Biology 2019Quote: ... Cells were transfected using Effectene (Qiagen) according to instructions provided by the manufacturer ...
-
bioRxiv - Neuroscience 2020Quote: Cells were collected with qiazol (Qiagen) and stored at -80°C until further processing ...
-
bioRxiv - Biochemistry 2021Quote: Cells were collected in QIAzol (Qiagen) and snap frozen in liquid nitrogen ...
-
bioRxiv - Cell Biology 2021Quote: ... or RNeasy Mini Kit (Qiagen; cells). cDNA was synthesized using 1 μg of mRNA using the SuperScript™ III Reverse Transcriptase (Invitrogen™ # 18080044 ...