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Citations for Qiagen :
1701 - 1750 of 6677 citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Human DNA from whole cell extracts of A549 cells was purified using the DNeasy Blood and Tissue kit (Qiagen). RNAseA (Thermo ...
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bioRxiv - Developmental Biology 2023Quote: ... genomic DNA from sorted cell pellets was extracted using the QIAGEN Blood & Cell Culture DNA Maxi Kit (QIAGEN; 13362) and quantified by Qubit (Thermo-Scientific ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions from SPL and BM were subjected to red blood cell lysis (erythrocyte lysis buffer, EL; Qiagen). Monoclonal antibodies (mAbs ...
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bioRxiv - Cancer Biology 2023Quote: ... red blood cells were lysed in five times the amount of red blood cell lysis solution (Qiagen, Hilden, Germany), followed by 15 min of incubation at room temperature (24°C) ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA (gDNA) was extracted from J-Lat cells 10.6 and HIV-1 negative CD4+ T cells with the QIAcube (Qiagen), using the QiaAmp DNA mini kit (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was extracted from isolated cells (microglia or non-microglia cells) with RNeasy RNA Isolation kit (Qiagen 74104) and used to generate cDNA with a High-Capacity cDNA Reverse Transcription Kit (Thermo 4368814) ...
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bioRxiv - Genetics 2023Quote: ... genomic DNA from sorted cell pellets was extracted using the QIAGEN Blood & Cell Culture DNA Maxi Kit (QIAGEN; 13362) and quantified by Qubit (ThermoScientific ...
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bioRxiv - Genomics 2023Quote: Genomic DNA from each cell was amplified using the Repli-G Advanced DNA Single-Cell kit and protocol (QIAGEN) for amplifying purified genomic DNA ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was extracted from 5-10×106 cells using the Gentra Puregene Cell Kit (Qiagen, cat no. 158046) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... total RNAs from EV preparations and 0.5 × 106 cells were extracted using the miRNeasy Tissue/Cells Advanced Micro Kit (QIAGEN), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... genomic DNA was extracted from bulk-transduced cells that did not undergo HCR using the Puregene Cell Kit (Qiagen). Meanwhile ...
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bioRxiv - Microbiology 2020Quote: ... and then cell lysates were collected by washing cells twice with PBS and adding 100ul of RNA lysis buffer (Qiagen) to each well.
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested by centrifugation at 4°C followed by cell lysis/RNA extraction using RNEasy plus mini kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: One colony per clone corresponding to ∼2×104 cells and 2×103 primary cells of each individual were lysed in RLT Plus (Qiagen) and stored at −80°C until processing ...
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bioRxiv - Immunology 2021Quote: ... and CD4 memory T cells were sorted on a FACSAria III cell sorter (Becton Dickinson) into QIAzol Lysis Reagent (QIAGEN). A total of 100,000 cells was sorted per sample ...
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bioRxiv - Genetics 2021Quote: ... were processed for DNA extraction using the Genomic-tip 20/G (mini prep, starting with approx. 108 cells) or 100/G (midi prep, 109 cells) kit (QIAGEN), according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2020Quote: WGA of single cells was performed by multiple displacement amplification using the REPLI-g Single Cell kit (Qiagen, Valencia, CA) as per the manufacturer’s instructions but at one quarter of the recommended volumes ...
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bioRxiv - Molecular Biology 2021Quote: ... MDA was done directly on cells (Supplementary figure 9) using the REPLI-g Single Cell Kit from Qiagen (Cat #150343) following kit-specific protocol ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was extracted from MTX-resistant HT-29 cells and control samples using the Gentra Puregene Cell kit (Qiagen) and the library for PacBio ...
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bioRxiv - Immunology 2021Quote: RNA was extracted from the cell pellets of human primary male and female PT cells using the RNAeasy Mini Kit (Qiagen). After quantifying RNA concentration in a Nanodrop instrument (Thermo) ...
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bioRxiv - Microbiology 2021Quote: ... a volume equivalent to 5×108 cells were spun down (assuming 1 OD600=8×108 cells) and Protect Bacteria RNA Mini Kit (Qiagen) was used to extract total RNA as described in (Bhattacharyya et al. ...
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bioRxiv - Neuroscience 2020Quote: DNA and RNA from FAC-sorted cells were isolated simultaneously from the same cell pellet using an AllPrep DNA/RNA Micro Kit (Qiagen) with on-column DNase treatment during the RNA isolation ...
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bioRxiv - Genetics 2021Quote: ... These single cells or nuclei were whole-genome amplified by a REPLI-g Single Cell Kit (Qiagen, Venlo, the Netherlands) for 3 hours ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA (gDNA) was extracted from the isolated cells and unsorted cells (“Input”) with QIAamp DNA Maxi kit (Qiagen, #51104).
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bioRxiv - Microbiology 2020Quote: ... total DNA from 2×106 of Burkitt cells was extracted by the Blood & Cell culture DNA mini kit (Qiagen #13362). For extracellular viral DNA extraction ...
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bioRxiv - Cell Biology 2021Quote: ... After the last irradiation cells were given 24 hours to recover and gDNA was isolated using the Blood & Cell Culture DNA Midi Kit (Qiagen) according the manufacturers protocol (DNA content of MRC-5 cells was estimated at 10 pg per cell ...
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bioRxiv - Cell Biology 2021Quote: Total mRNA was isolated from HEK293 (2×106) and Jurkat cells (4×106) parental and MCU-KO cell lines using the RNeasy Mini Kit (Qiagen). Isolated RNA was then analyzed using a NanoDrop spectrophotometer (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: RNA from infected cell lysates was harvested by incubating infected Vero E6 cells for 10 minutes with RLT buffer (Qiagen) containing 2-Mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was extracted from tissues (CC) or cells (microglial GFP+ cells sorted by FACS) using the RNeasy Mini Kit (Qiagen). After reverse transcription using Superscript First-Strand Synthesis System (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... shRNA expressing cells were isolated by fluorescence activated cell sorting at passage doubling 1 and 15 followed by genomic DNA isolation with Gentra Puregene cell kit (Qiagen). Sequencing libraries were prepared by subjecting 18 μg (1.2 μg x 15 reactions ...
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bioRxiv - Immunology 2022Quote: Clonal naïve GMP and neutrophil total RNA was extracted from cells at 5×106 cells/ml using the Qiagen RNeasy Plus Mini Kit (Qiagen) with DNaseI treatment (RNase free DNase Set ...
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bioRxiv - Immunology 2019Quote: Total RNA was extracted from sputum cell pellets or bronchial epithelial cell lysates stored in RLT buffer (RNeasy kit, Qiagen) and 2μg was used for cDNA synthesis (Omniscript RT kit) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then harvested and subjected for genomic DNA isolation using “Blood & Cell Culture DNA Mini Kit” (Qiagen, Hilden, Germany). Reactivation of LCLs was determined by real-time quantitative PCR (qPCR ...
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bioRxiv - Immunology 2019Quote: DNA and RNA were extracted from cells using either Blood & Cell Culture DNA Mini Kit and RNAeasy isolation kit (Qiagen), or all-prep DNA/RNA kit (Qiagen) ...
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bioRxiv - Immunology 2020Quote: RNA from isolated fresh B cells and B cells activated with LPS/IL-4 was extracted using a RNeasy kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted from wild-type and ΔTMPRSS2 293T cell lines using the Puregene cell kit according to the manufacturer’s instructions (Qiagen, #158388). Co-transfection of pJC144 (guide 1 ...
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bioRxiv - Microbiology 2020Quote: ... or experimental siRNA 72 h after transfection (approximate cell concentration was 1.6×105 cells/ well) were harvested and lyzed using the QIAshredder Kit (Qiagen; 79656). mRNA was harvested using the RNeasy Plus Micro Kit (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... was extracted from the top (5.61 million cells) and bottom (4.87 million cells) fractions using the QIAamp DNA mini kit (QIAGEN cat.#51304). Fixed cells were decrosslinked by overnight incubation in 200 µL of PBS with added Proteinase K and 200 µL buffer AL (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: RNAs were prepared from purified D0 cells and from D7 to D14 populations isolated by flow cytometric cell sorting using an RNeasy Plus kit (Qiagen), and then depleted of rRNAs and processed for RNA-Seq library construction using a TruSeq Stranded mRNA Library Prep Kit (Illumina) ...
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bioRxiv - Cancer Biology 2021Quote: ... gDNA was isolated from at oncogene-negative mouse cell lines or FACS-sorted Tomatopositive cancer cells using either the AllPrep DNA/RNA(Qiagen) or the DNeasy Blood and Tissue Kit ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... and CD45+ Calcein+ DAPI-CD19+ CD38+ activated B cells were single-cell sorted into 96-well plates with catching buffer (RLT lysis buffer (Qiagen) with 1% 2-mercaptoethanol (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were removed and the remaining cell pellet (500 μl) was transferred into a 15 ml tube containing 2.5 ml RNAprotect® Cell Reagent (Qiagen). The cells were resuspended by vortexing ...
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bioRxiv - Microbiology 2021Quote: Total RNA (5×106 cells) was extracted from FEA or CRFK cell lines by using the RNeasy Plus Mini Kit (Qiagen). RNA from FEA ...
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bioRxiv - Immunology 2021Quote: Human B cells harvested post gene-editing or cell sorting were lysed for RNA extraction by the RNeasy Micro Kit (74004, Qiagen). Primers used for reverse transcription and library amplification were modified from previous29 ...
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bioRxiv - Genomics 2021Quote: ... DNA was isolated from thawed APOE cell lines by Bioneer A/S using the Blood & Cell Culture DNA Mini Kit (Qiagen). For analysis of Pax8-CreERT2 insert high molecular DNA was prepared from mouse ears using MagAttract HMW DNA Kit (Qiagen ...
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from cultured in vitro or in vivo primed T cells or ex vivo sorted T cells using the RNeasy kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... high molecular weight genomic DNA (gDNA) was purified from human primary T cells using the Blood & Cell Culture DNA Maxi Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA (gDNA) isolation from bulk transfected cells and clonal cell lines was performed using the DNeasy Blood and Tissue kit (Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA from the FACS-purified cells was isolated immediately after FACS sorting using Qiagen RNA Mini (>100,000 cells, Qiagen 74104) or RNA Micro kits (<100,000 cells ...
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bioRxiv - Microbiology 2022Quote: RNA was isolated from approximately 107 cells grown to a density of 106 cells/ml using the RNAeasy Mini Kit (Qiagen), including an on-column DNase I treatment (Qiagen) ...