Labshake search
Citations for Qiagen :
101 - 150 of 797 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2024Quote: ... 500 µL AP1 lysis buffer and 10 µL proteinase K (10 mg mL-1; Qiagen) were added to each tube ...
-
bioRxiv - Immunology 2021Quote: ... Pup stomach contents and intestines were collected and homogenized in 500 µm PBS using a TissueLyzer II (Qiagen) for 5 min at 50 Hz with a stainless steel ball added as a pulverizer ...
-
bioRxiv - Biochemistry 2023Quote: ... Clarified lysate was then filtered through a 0.45 µm syringe filter and mixed with 1.5 mL of washed Ni-NTA Agarose beads (Qiagen). The mixture was allowed to bind under nutation at 4℃ for 2 hr ...
-
bioRxiv - Plant Biology 2023Quote: ... 500 ng of DNA nuclease-treated RNA was combined with 1.05 mM dNTPs and 10.5 µM random hexamers (Qiagen) in a reaction volume of 14.25 µL before incubation at 65°C for 5 minutes ...
-
bioRxiv - Microbiology 2022Quote: ... 1.9 µL of 10× buffer (Qiagen), and 16.5 µL of water ...
-
bioRxiv - Microbiology 2021Quote: A 10 mL empty column (Qiagen) was packed with 1 mL of Ni-NTA superflow (Qiagen) ...
-
bioRxiv - Microbiology 2024Quote: ... 10 mL RNAprotect bacteria reagent (Qiagen) was passed through the filter ...
-
bioRxiv - Biochemistry 2021Quote: ... and the membrane suspension was mixed with 1 ml of Ni-NTA Superflow resin (Qiagen) per 1mg of GFP–His8 and incubated for 3 hours at 4 °C ...
-
bioRxiv - Genetics 2020Quote: ... the repair template plasmid was prepped using silica-membrane based kit (Plasmid Miniprep Kit, Qiagen) and sent off for Sanger Sequencing to confirm proper assembly of the plasmid.
-
bioRxiv - Biophysics 2024Quote: ... membranes were incubated under soft shaking with Penta-His antibody (Penta His HRP Conjugate; Qiagen) for 1 h with 0.5 % (w/v ...
-
bioRxiv - Microbiology 2024Quote: ... The EV RNA was then isolated through a membrane affinity exoRNeasy Midi Kit (Qiagen, 77144), according to the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2020Quote: ... we differentiated human stem cells for 3 days in mTeSR + 0.5 µM A8301 and extracted RNA with RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... Pyrosequencing of the PCR products was performed using 0.5 µM of sequencing primer on the PSQ96 HS System (Pyrosequencing, Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2022Quote: ... 10 ng of each sample (technical triplicates of 3 independent biological replicates) was amplified using various primers (0.5 µM; Table S2) and SYBR Green PCR master mix (QIAGEN). Reactions were run for 44 cycles on a StepOnePlus Real-Time PCR machine (Applied Biosystems ...
-
bioRxiv - Genetics 2020Quote: ... AC7594-5’-CCGCCTATCCTCGTCATGAAC)andcontrolALG9primers(AC5067-5’-CACGGATAGTGGCTTTGGTGAACAATTAC;AC5068-5’-TATGATTATCTGGCAGCAGGAAA GAACTTGGG) (0.5 µM) and QuantiTect SYBR Green PCR master mix (Qiagen) on a StepOnePlus Real-Time PCR machine (Applied Biosystems ...
-
bioRxiv - Microbiology 2020Quote: Following 72 h post treatment with a sublethal concentration of compound 33 (3.13 µM), adult male worms (n = 20 worms, three biological replicates) were homogenized with a TissueLyser (Qiagen) and total histones extracted using the EpiQuikTM Total Histone Extraction kit (OP-0006 ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... The PCR mix contained 0.16 µM of each of the fluorescent universal primer and of the reverse specified primer and 0.04 µM of the 5’ tail forward primer in a final 15 µl reaction volume (2x QIAGEN Multiplex PCR Master Mix with 3 mM Mg2+ ...
-
bioRxiv - Biochemistry 2024Quote: ... The supernatant was filtered through a 0.45 µm syringe filter and applied to a 5 ml Ni-NTA agarose column (Qiagen) pre-equilibrated in Lysis-Wash buffer ...
-
bioRxiv - Biochemistry 2024Quote: ... Supernatants were filtered through a 0.22 µm syringe filter before application to 5 ml of nickel resin (Ni-NTA Superflow, QIAGEN) equilibrated in loading buffer (25 mM HEPES-KOH pH 7.6 ...
-
bioRxiv - Cell Biology 2023Quote: ... The supernatant was 0.2 µM filtered and was incubated overnight at 4°C with 5 mL of Ni-NTA beads (Qiagen). XXX
-
bioRxiv - Cell Biology 2024Quote: ... the cell pellet was suspended in 50 µM propidium iodide and 20 × diluted RNase (19101, QIAGEN Inc., Venlo, Netherlands) and then were incubated at 37°C for 30 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... the proteins were transferred to nitrocellulose membranes before being revealed with anti-Histidine monoclonal antibodies (Qiagen). Immune complexes were detected with anti-rabbit peroxidase-conjugated secondary antibodies (Promega ...
-
bioRxiv - Microbiology 2022Quote: ... the supernatant was filtered with 0.22-um membrane filter and loaded on Ni-NTA agarose (Qiagen) and ...
-
bioRxiv - Microbiology 2022Quote: ... This was accomplished using silica membrane spin columns (DNeasy PowerClean Pro Cleanup Kit; Qiagen 12997-50), before elution in 10 mM Tris-Cl buffer (pH 8.5) ...
-
bioRxiv - Molecular Biology 2023Quote: ... but additionally performing DNase digestion on the RNeasy column membrane with RNase-free DNase set (Qiagen) for 30 min after wash by RW1 buffer ...
-
bioRxiv - Bioengineering 2024Quote: ... RNA was lysed directly from the PDMS membrane using the RNeasy Mini Kit (Qiagen, Hilden, Germany). RNA concentration and purity were evaluated using a NanoDrop OneC (ThermoFisher Scientific ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 10 μL of the RDD buffer (Qiagen), 2.5 μL of DNase I (Qiagen ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 10 μl of HotStarTaq Master Mix (Qiagen), 0.08 μM of each primer ...
-
bioRxiv - Genetics 2022Quote: ... for 10 minutes using a TissueLyserLT (Qiagen), then incubated on ice for 30 minutes ...
-
bioRxiv - Genomics 2023Quote: ... 10 µL of RLT+ lysis buffer (Qiagen) was then added to each well and the plate was sealed and centrifuged (2000 x g ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2.7 × 10−5 AU/mL protease (Qiagen), 1.0 μM barcoded oligodT-ISPCR primer ...
-
bioRxiv - Microbiology 2024Quote: ... 10 μg/ml RNase-free DNase (Qiagen), and 1 uL/ml of RNaseOUT (Thermo Fisher) ...
-
bioRxiv - Microbiology 2024Quote: ... 10 ml of RNAprotect Bacteria Reagent (Qiagen) was added to cultures ...
-
bioRxiv - Molecular Biology 2020Quote: ... using 1 µl of the cDNA product as template and either Fast Cycling PCR kit or HotStarTaq Plus Master Mix with a final concentration of 0.1 µM for each primer following the manufacturer’s protocols (Qiagen, Hilden, Germany). PREDICT universal controls 1 and 2 (Anthony et al. ...
-
bioRxiv - Microbiology 2023Quote: Three individual colonies of WT and Pcu-shc::Δshc growth with and without 25 µM cumate were cultured to early exponential phase and total RNAs were extracted by Qiagen RNeasy Mini Kit as described previously (44) ...
-
bioRxiv - Microbiology 2023Quote: Genomic DNA and total RNA of microbial biomass from the 0.2 µm filters were extracted using the DNeasy PowerSoil kit (Qiagen, Germany) and the RNeasy PowerWater kit (Qiagen ...
-
bioRxiv - Molecular Biology 2023Quote: ... Oligonucleotides were incubated with a PCR mix containing (100 µM dNTP, 1X thermopol Taq reaction buffer, 1X of Q solution (Qiagen) and 1 µM of each primer) ...
-
bioRxiv - Immunology 2024Quote: ... Expression of target ncRNAs in CH12F3 and primary B cells were inhibited by 2 µM miRCURY LNA miRNA Inhibitors (339131, Qiagen), anti-miR-5099 (GGAGCACCACATCGATCTAA-FAM) ...
-
bioRxiv - Immunology 2024Quote: ... Overexpression of miR-5099 in CH12F3 was achieved by transfecting 2 µM of miR-5099 miRCURY LNA miRNA mimic (Qiagen) by electroporation using Lonza 4D-Nucleofector and cell line SF kit following manufacturer’s protocol.
-
bioRxiv - Genetics 2024Quote: ... 20 µM of Illumina primers and 1:2000 dilution of 2 ng/µl library on Rotor-Gene Q machines (Qiagen), program and primer sequences are in Table) ...
-
bioRxiv - Genetics 2024Quote: ... RNA was isolated from cultures incubated or not with 1 µM staurosporine for 6 hours to induce apoptosis using the RNeasy Mini Kit (QIAGEN). RNA samples were checked for quality on Agilent 4200 TapeStation System (Agilent Technologies ...
-
bioRxiv - Genetics 2020Quote: ... Tissue DNA was digested on the RNeasy spin column membrane using the RNase-Free DNase Set (QIAGEN) and RNA integrity was confirmed by agarose gel electrophoresis ...
-
bioRxiv - Cell Biology 2021Quote: Total RNA was isolated using a silica membrane RNA purification method (RNeasy Mini Kit, Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... DNA was extracted from these membranes using the DNeasy PowerWater kit (cat# 14900-100-NF, Qiagen, USA), using the protocols provided by the manufacturer ...
-
bioRxiv - Molecular Biology 2022Quote: ... Protein bands were transferred to a nitrocellulose membrane and probed with primary antibodies [mouse anti-His (Qiagen) to detect CAT ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins transferred to a nitrocellulose membrane were probed with a monoclonal anti-His6 antibody (Qiagen, Germantown, Maryland). Detection of proteins via Western blotting was performed by fluorescence detection using IR-Dye®-labeled fluorescent secondary antibodies and imaged using the Odyssey CLx Imager (LICOR Biosciences ...
-
bioRxiv - Microbiology 2023Quote: The airway epithelium cultured on 6.5 mm transwell membranes was washed and treated with RLT buffer (Qiagen) with 1% β-mercaptoethanol (Sigma‒Aldrich) ...
-
bioRxiv - Biochemistry 2024Quote: ... the membranes were treated with 1:3000 dilution of Qiagen anti-Penta His HRP conjugate (Qiagen 34460) and excess antibody removed with 3 washes in PBST for 20’.
-
bioRxiv - Evolutionary Biology 2024Quote: ... This modification was conducted prior to loading the membrane digests into an automated QIAcube (Qiagen; Venlo, Netherlands) DNA extraction system ...
-
bioRxiv - Systems Biology 2020Quote: ... and RNA seqFISH primary probe pools (1-10 nM per probe) and 10 nM polyT LNA oligo with a readout probe binding DNA sequence (Qiagen) were hybridized in 50% hybridization buffer consisted of 50% formamide (Invitrogen AM9342) ...