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Citations for Qiagen :
1251 - 1285 of 1285 citations for Lysozyme C LYZ Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Buffer G2 and proteinase K were incubated at 56°C overnight and extracted using the EZ1 DNeasy Blood Tissue Kit (Qiagen GmbH, Hilden, Germany) in line with the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: Data to derive different biological clocks was available for different subsamples and all based on a fasting blood draw from participants in the morning between 8:30 and 9:30 after which samples were stored in a −80°C freezer or – for RNA - transferred into PAXgene tubes (Qiagen, Valencia, California, USA) and stored at −20°C ...
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bioRxiv - Cell Biology 2022Quote: ... The reaction mixture was incubated at 37°C for 24 hours and subsequently underwent RNA clean-up using a column-based purification (Qiagen, product number 74104). The purified RNA was eluted in nuclease-free water (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... Efficient electroporation was confirmed by observation of fluorescence before genomic DNA extraction around 16 hpf (18°C) using QIAamp DNA Micro kit (Qiagen, German Town, MD). Mutagenesis efficacy of individual sgRNAs ...
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bioRxiv - Neuroscience 2021Quote: ... The mixture was incubated at 37 °C for various times and the DNA was purified using a MinElute PCR purification kit (Qiagen, Germantown, MD, USA). Then ...
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bioRxiv - Molecular Biology 2022Quote: ... and the DNA pellet was stored at −80°C until purification was performed using a commercial kit (DNeasy Blood and Tissue Kit, catalog #69504; Qiagen; Germantown, MD, USA) according to manufacturer’s instructions with a minor modification to the elution step ...
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bioRxiv - Microbiology 2022Quote: ... strain SPR117 was grown during 7 days in R2A broth at 20 °C and its genomic DNA (gDNA) was extracted using the Dneasy UltraClean Microbial Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Brain parts were stored in 100 µL of Trizol™ in -80 °C for later RNA extraction using RNAeasy Mini Extraction Kit™ (Qiagen, Germany) according to the manufacturers protocol ...
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Macrobdella decora: Old World Leech Gut Microbial Community Structure Conserved in a New World LeechbioRxiv - Microbiology 2019Quote: ... The supernatant was transferred to clean 1.5mL MCF tube and to which 350 μL ethanol at 4’C was added before vortexing for 10 sec and transferring the supernatant to a QIAamp Mini spin column (Qiagen Germantown, MD U.S.A.). The sample was process as recommended in the QIAamp DNA Mini Handbook and eluted with 10 mM Tris-Cl ...
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bioRxiv - Microbiology 2019Quote: ... from the bacterial cell pellet of input library and output libraries stored at −80°C was extracted using QIAamp DNA Mini Kit (Qiagen, Valencia, CA, USA) following manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... individuals were flash frozen in liquid nitrogen and stored in −80°C until DNA extraction using the Qiagen DNeasy Plant kit (Qiagen, Valencia, CA, USA) as described in (Kainer et al ...
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bioRxiv - Biochemistry 2021Quote: ... Medium was stirred for 1 hour at 4°C in the presence of 5 ml Ni-NTA Agarose beads (Qiagen, Venlo, The Netherlands) washed with 25 mM Hepes-KOH pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The soluble fraction was separated from debris by centrifugation (9000 g, 4°C) and incubated for 60 minutes with Ni-NTA beads (Qiagen, cat. no. 30230) pre-equilibrated in binding buffer ...
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bioRxiv - Microbiology 2023Quote: ... approximately 1 x 104 colonies were collected and pooled into LB-amp culture media and then incubated at 37°C for five hours prior to harvesting the bacterial population for plasmid purification by Maxiprep (Qiagen Plasmid Maxi Kit). The presence of a diverse barcode in the plasmid stock was verified by next generation sequencing (see below) ...
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bioRxiv - Molecular Biology 2024Quote: Total bacterial DNA was extracted from frozen (−80°C) fecal samples from AuPhos- or vehicle treated mice using PowerFecal DNA/RNA Kit (Qiagen, Hilden, NRW, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: Isolated human LGR5+ cells were stored at −80°C until processed for RNA isolation using RNeasy micro kit as per manufacturer instruction (Qiagen, Germantown, MD, USA). RNA processing ...
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bioRxiv - Cancer Biology 2023Quote: Cells were harvested (1×106) by centrifugation (200 x g for 5 min at 25°C. Whole RNA was isolated using the Qiagen RNeasy kit (Qiagen) according to the manufacturer’s description ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellets were kept at −80°C until performing the DNA extraction using DNAeasy Blood & Tissue Kit (QIAGEN; Cat. No. ID: 69504). Qubit Broad Range double-stranded DNA assay (Life Technologies ...
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bioRxiv - Biophysics 2024Quote: ... for 30 min at 4 °C before being applied to a 10mL bed volume Nickel nitrilotriacetic acid (Ni-NTA) agarose (Qiagen, cat. no. 30230) column ...
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bioRxiv - Molecular Biology 2024Quote: ... Liquid cultures were grown at 37°C overnight to saturation and DNA was extracted using QIAGEN® Plasmid Plus Midi Kit (Qiagen cat#12945)
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bioRxiv - Molecular Biology 2020Quote: ... Animals were raised at 25°C for 48 h after hatching and total RNA was purified by using RNeasy® Mini Kit (Qiagen, Venlo, The Netherlands). Microarray and the following data analysis were performed as described before (Qi et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Enzyme was inactivated at 95 ° C for 10 min and 700 μl Trizol was added followed immediately by RNA extraction using the miRNeasy kit (Qiagen Inc., Valencia, CA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... Both the input and IP samples were incubated at 50°C for 2 hr and then cleaned up using the Qiaquick PCR Purification Kit (Qiagen, Hilden, Germany, Cat # 28104) and eluted in 35 μL of water.
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bioRxiv - Evolutionary Biology 2019Quote: ... with 5 µl proteinase K (20 mg/ml) and lysed overnight at 56°C under shaking in Cell Lysis Solution (Qiagen Gentra Puregen Cell kit) with proteinase K (20 mg/ml) ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was prepared from synchronized day one adult animals raised at 20°C by using RNeasy® Mini Kit (Qiagen, Venlo, The Netherlands). Oligo-dT primer was used to synthesize cDNA by using Transcription First Strand cDNA synthesis kit (Roche ...
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bioRxiv - Genetics 2024Quote: The organs of Corti of postnatal day (P)4 mice were dissected and stored at -20°C in RNAprotect Tissue Reagent (RNAlater®) (QIAGEN, cat. no. 76106). Total RNA was extracted using the SPLIT RNA extraction kit (Lexogen ...
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bioRxiv - Plant Biology 2023Quote: ... All soil samples were stored at –20°C until rhizosphere DNA was extracted using a QIAGEN DNeasy PowerSoil Kit (Qiagen, Inc., Valencia, CA, USA) following the manufacturer’s protocol.
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bioRxiv - Systems Biology 2020Quote: ... was prepared by centrifuging a cell culture at 5525xg for 2 minutes at 4°C then resuspending cells in 1mL of PBS-RNAprotect (333 μL RNAprotect Bacteria Reagent [76506, Qiagen, Hilden, Germany], 666 μL PBS). For immediate RNA stabilization by RNAprotect (Fig ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed twice with the blocking buffer and incubated at 4 °C with primary antibodies (mouse anti-Strep, Qiagen, Cat #: 34850, 1: 150 dilution) (rabbit anti-PDI ...
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bioRxiv - Genomics 2022Quote: An aliquot of 150 μL of plasma per sample was thawed on ice and centrifuged at 3000 × g for 5 min at 4 °C and smallRNA was extracted using miRNeasy Serum/Plasma Kits (Qiagen, Cat. No. 217184, Milano, Italy) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was extracted from homogenates after overnight incubation with 20 μl of proteinase K at 56°C using the QIAamp DNA Micro Kit (Qiagen, Hilden, Germany, cat. No. 56304) following the manufacturer’s protocol for tissue samples ...
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bioRxiv - Cell Biology 2023Quote: ... RNA and protein were digested in 40 mM Tris-HCl pH 6 and 10 mM EDTA by adding first RNAse A (Qiagen; #19101, 1.5 hours, 37°C), followed by Proteinase K (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... The remaining cells were pelleted by centrifugation (4000rpm, 30min, 4C) and stored at –20°C for bulk EEV extraction by endotoxin-free maxiprep (Qiagen EndoFree Maxi Kit, Cat. 12362).
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bioRxiv - Molecular Biology 2024Quote: ... DNA pellets were washed 3X with 70% ethanol and hydrated in pre-warmed (37°C) Elution buffer (Qiagen, 10 mM Tris-Cl, pH 8.5. Cat. 19086) and incubated on HulaMixer™ Sample Mixer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... 5’ and 3’ untranslated regions and open reading frames) for the paralogs were made using the CLC Main workbench 7 (QIAGEN® Aarhus A/S, Aarhus C, Denmark). Sequence alignments were generated for the genomic DNA ...