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Citations for Qiagen :
1101 - 1150 of 1234 citations for Lysozyme C LYZ Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... The resultant nuclei were then incubated for 30 min at 37°C with an in-house made Tn5 enzyme and purified with the Qiagen MinElute kit (Qiagen, 28004). PCR reactions for each replicate were performed with 15 cycles using Ad1F and Ad2.2R primers and NEBNext High-Fidelity 2X PCR Master Mix (New England Labs Cat #M0541) ...
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bioRxiv - Microbiology 2019Quote: ... 1 µM of forward and reverse oligos were heated for one minute at 100°C with 5 mM MgCl2 and 7 mM Tris-Cl (i.e. Qiagen Elution Buffer) and annealed by slowly cooling to room temperature.
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bioRxiv - Molecular Biology 2021Quote: ... the PCR products were held at 4°C until PCR purification using the QIAquick PCR purification kit (#28106, Qiagen, Hilden, Germany). DNA concentrations were determined using NanoDrop 2000 (Thermo Scientific) ...
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bioRxiv - Genomics 2022Quote: ... The resulting pellet was gently resuspended in 20 ml of pre-warmed (37°C) G2 lysis buffer (Qiagen; Cat. no. 1014636), incubated with 50 μg/ml RNaseA (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... harvested and stored at −80°C in RLT buffer with β-mercaptoethanol from the RNeasy Mini prep Kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions until cDNA synthesis for RT2 Profiler PCR array ...
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bioRxiv - Microbiology 2021Quote: ... Fecal pellets were snapfrozen and stored at −80°C until DNA extraction with the DNeasy Powersoil kit by Qiagen (Hilden, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Transposition was performed for 30 minutes at 37°C after which the transposed DNA was purified using MinElute PCR Purification Kit (Qiagen, 28004) and eluted in 10 µl of elution buffer (EB ...
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bioRxiv - Neuroscience 2020Quote: ... 100μL chloroform were added and samples spun at 7000rpm for 15min at +4°C in a MaXtract column (Qiagen, Venlo, Netherlands). Mixed with 500μL 70% EtOH and spun at 10 000rpm for 30s at +4°C in a MiniElute column ...
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bioRxiv - Genomics 2022Quote: ... Cells were maintained at 37°C in 5% CO2 for 3 days before collecting genomic DNA using DNeasy Blood & Tissue Kits (Qiagen, 69504) and sequencing.
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bioRxiv - Microbiology 2019Quote: ... the supernatant was removed with a micropipet and 350 µL of 4°C Buffer RLT Plus (from Qiagen RNeasy Mini Kit) was added to the pellet to collect the RNA ...
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bioRxiv - Zoology 2020Quote: ... SDS and 100 µg/ml proteinase K) and incubated at 56°C for 1 h before extraction using a QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer’s directions ...
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bioRxiv - Genomics 2020Quote: ... Dehydrated samples were stored at −80 °C until resuspension in 25 μl or 100 μl of Buffer EB (QIAGEN, Venlo, Netherlands).
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bioRxiv - Synthetic Biology 2020Quote: ... and ∼55 mg of total protein in CFE was incubated for 60 min at 4 °C with 2 mL of Ni-NTA agarose (QIAGEN, Germany) equilibrated with purification buffer A ...
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bioRxiv - Immunology 2020Quote: ... Pellets were lysed by vortexing for 1 minute in 350uL cold supplemented RLT buffer (RLT + β-MeOH) at 4°C and lysates homogenized using QIAshredder columns (Qiagen). RNA was then extracted from these samples using the RNeasy Plus Micro kit (Qiagen ...
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bioRxiv - Neuroscience 2019Quote: ... Buffer RLT containing immunoprecipitated RNA was then eluted and stored at −80°C until clean up using a kit (Qiagen 154025593). cDNA was generated using 11 rounds of amplification with 10 ng RNA input.
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bioRxiv - Physiology 2019Quote: ... Methylation status of the individual CpG sites was identified as an artificial C/T SNP using QCpG software (Pyrosequencing, Qiagen, UK). Then % methylation of each CpG site was then calculated as a proportion of the methylated alleles divided by the sum of all methylated and unmethylated alleles ...
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bioRxiv - Biochemistry 2019Quote: ... genomic DNA was de-crosslinked in ChIP elution buffer containing 5 M NaCl at 65 °C overnight and purified with the Qiaex II kit (Qiagen, 20021) and eluted in water for PCR amplification ...
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bioRxiv - Neuroscience 2021Quote: ... Buffer RLT containing immunoprecipitated RNA was then eluted and stored at −80 °C until clean up using a kit (Qiagen 74004). cDNA was generated using 11 rounds of amplification with 10 ng RNA input ...
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bioRxiv - Cell Biology 2020Quote: Isolated Chromatin was digested with 1% proteinase K for 2 hrs at 60 °C with 300 rpm and was subsequently purified by using QIAquick® Nucleotide Removal Kit (28306, Qiagen) according to the manufactory’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Samples were centrifuged at 5000 g for 2 hours at 4 °C and the pellet was collected for DNA extraction with a DNeasy PowerSoil kit (Qiagen, Germany). The sediment from Lime Blue was collected with a freeze core (modified from Stocker and Williams ...
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bioRxiv - Bioengineering 2022Quote: ... after which the cells were FACS sorted for high and low reporter signal.The genomic DNA was extracted by lysing the cell pellets for 10 minutes at 56°C in AL buffer (Qiagen, 19075), supplemented with Proteinase K (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... The resulting 1.5 L bacterial culture was incubated at 37°C for 2h and a Maxiprep performed using Qiagen MaxiPlus kit (Qiagen #12963) to a resulting 1 mg of plasmid DNA ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The right pectoral fin was removed and stored in 95% ethanol at −20 °C prior to subsequent DNA extraction using QIAamp Fast DNA Tissue kits (Qiagen, Inc.) following the manufacturer’s protocols.
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bioRxiv - Genomics 2022Quote: ... After overnight incubation at 37 °C colonies were picked for mini-prep cultures and mini-preps were performed using the Qiaprep® Miniprep kit (Qiagen). The insert in the resulting plasmid was sanger sequenced (Source Biosciences ...
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bioRxiv - Plant Biology 2022Quote: ... Samples were centrifuged at 14,000 x g for 20 min (4°C) to pellet insoluble cell debris and supernatants were transferred to violet QIAshredder mini spin columns (Qiagen, Australia) and centrifuged again for one minute ...
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bioRxiv - Cell Biology 2022Quote: ... were digested with DpnI for 1 hour at 37°C and subsequently purified with the QIAquick Gel Extraction Kit (Qiagen #28704). They were ligated with homemade Gibson Master Mix (Table S1 ...
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bioRxiv - Cell Biology 2022Quote: ... Islets were pelleted and stored at −80°C until DNA extraction using the Blood/Tissue DNeasy kit (Qiagen; Germantown, MD, USA) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Genomic bacterial DNA was isolated from overnight grown cultures (LB, 37°C) by the DNA Extraction kit (DNeasy Kit, Qiagen Germany) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... dCas9-Halo protein was purified from the supernatant by incubating for 2 h at 4 °C with Ni-NTA agarose (Qiagen #30210). The beads were washed 10 times with 5 ml dCas9 wash buffer I (50 mM NaxPO4 pH 7.0 and 300 mM NaCl ...
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bioRxiv - Biophysics 2023Quote: ... dCas9-Cys protein was purified from the supernatant by incubating for 2 h at 4 °C with Ni-NTA agarose (Qiagen #30210). The beads were washed 10 times with 5 ml dCas9-Cys wash buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... The chromatin fragments were then left to reverse crosslink overnight at 65°C with shaking and the DNA was then purified with the QIAquick PCR purification kit (QIAGEN; 28104), according to manufactures instructions ...
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bioRxiv - Neuroscience 2023Quote: ... After protein digestion (PK Buffer and Proteinase K (20 mg/mL) for 2 hrs at 45°C) DNA was purified with QIAquick PCR Purification Kit (Qiagen, Germany) according to manufacturer instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... the supernatant was discarded and the pellet was frozen at -20 °C for further extraction with the DNeasy® Plant Mini Kit (Qiagen). Total DNA was eluted in 10 µl of water and concentration was measured using NanoDrop ...
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bioRxiv - Systems Biology 2023Quote: ... Liver samples were stored at -140°C and prepared as follows: total RNA was extracted using miRNeasy kit (Qiagen; Hilden, Germany). Libraries were prepared using 10 ng/μl with Truseq Stranded RNA ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were incubated at 37°C for 30 min and then purified using the MinElute Reaction Cleanup kit (Qiagen Cat #28204) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Single sorted cells (∼50’000 cells) were lysed in buffer RLT and stored at -80°C until starting the RNA isolation following manufacturer instructions (Qiagen, 74034). cDNA reaction was performed using qScript™ cDNA SuperMix (QuantaBio ...
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bioRxiv - Microbiology 2023Quote: ... and incubated at room temperature for 20 min Samples were then frozen at -20°C overnight prior to RNA extraction using the QIAamp Viral RNA Mini extraction kit (Qiagen, 52906) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei were incubated at 37°C for 60 minutes and fragmented DNA was purified using the MinElute kit (Qiagen, Cat #28206). Library preparation was performed on purified fragments using Nextera DNA Flex Library Prep Kit (Cat #20018704 ...
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bioRxiv - Immunology 2023Quote: For protein production a pT350 plasmid encoding for the construct of interest with a BiP signal sequence in the N-terminal and a double strep-tag in the C-terminal region was co-transfected with a puromycin resistant plasmid (pCoPuro) (61) with effectene reagent (Qiagen, 301425) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... following an incubation at 55°C for 1 hr in 450 µl of lysis buffer with proteinase K (QIAGEN, Hilden, Germany). Each colony was genotyped using twelve microsatellite loci69 ...
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bioRxiv - Zoology 2023Quote: ... The mixtures were incubated at 56 °C for 1 h before DNA was extracted using an QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Cells were grown in LB at 37°C with shaking and genomic DNA was isolated using the DNeasy® Tissue Kit (Qiagen) from exponential and stationary phase cultures ...
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bioRxiv - Neuroscience 2023Quote: ... Transposition occurred at 37°C for 30 minutes and transposed DNA was purified using the MinElute PCR Purification Kit (Qiagen, 28004). Indexing libraries using Nextera i5 and i7 indexed amplification primers (Illumina XT index kit ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA was extracted from fresh specimens stored at -20° C in 95% ethanol with a Qiagen DNeasy kit (Qiagen, Hilden, Germany) following manufacturer’s protocols modified by the recommendations of Cruaud et al. ...
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bioRxiv - Synthetic Biology 2024Quote: ... of T4 ligation reaction mix was added to NEB Stbl cell (C3040) for transformation and grown at 37°C for the plasmid DNA preparation (Qiagen miniprep). The resulting plasmids were sequence-verified using Sanger sequencing (Genewiz).
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bioRxiv - Developmental Biology 2024Quote: ... two independent limbs were microdissected and snap-frozen at −80°C for subsequent total RNA extraction using the RNEasy Mini Kit (QIAGEN, 74134) following protocol ...
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bioRxiv - Microbiology 2024Quote: ... and 10 zirconium beads were added to approximately 5 mg of intestinal tissue stored at -80 °C in RNA later tissue reagent (Qiagen, Germany). A Tissue lyser (Qiagen ...
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bioRxiv - Synthetic Biology 2021Quote: ... Colonies that grown on the LB-antibiotic plate were picked and inoculated into fresh LB-antibiotic medium at 37 °C to prepare overnight culture for plasmids extraction using QIAprep Spin Miniprep kit (Qiagen, Hilden, Germany). The plasmids were then sent for DNA sequencing (1st BASE ...
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bioRxiv - Biophysics 2021Quote: ... The capture probes contained an 11- to 12-nt target-capturing sequence with 4 LNA residues (Tm = ~70°C, estimated using Qiagen web application) for high affinity and kinetically stable capturing of nucleic acid targets on the surface ...
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bioRxiv - Developmental Biology 2021Quote: ... Tubes were placed at 37°C for 30 min and the resulting fragmented DNA was purified using the Minelute Cleanup Kit (Qiagen, Hilden, Germany), with elution performed in 10 µl of the provided elution buffer ...