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Citations for Qiagen :
801 - 850 of 4884 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... The PCR product was then desalted and purified using the Qiaquick PCR purification kit (Qiagen). Next-generation sequencing was performed using the Illumina MiSeq system with 500 cycles ...
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bioRxiv - Molecular Biology 2024Quote: ... The second PCR products of each pool were purified by QIAquick PCR Purification Kit (Qiagen). RNAseq samples were added to 10% to create balanced reads for the first 21 nucleotides sequenced ...
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bioRxiv - Microbiology 2024Quote: ... and PCR amplicons were purified using the Min-elute 96 UF PCR purification kit (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Biophysics 2024Quote: ... The PCR products were then column purified using the QIAquick PCR Purification Kit (Qiagen #28104) and run out on a 2% E-Gel agarose gel (Thermo Scientific #A42135 ...
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bioRxiv - Biochemistry 2024Quote: ... Standards were generated by purifying the PCR-product (MinE-lute PCR Purification Kit, Qiagen, Germany) and 10-fold serial dilution ...
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bioRxiv - Genetics 2021Quote: ... Dissected kidneys were stored in RNAlater solution (Qiagen) and kept frozen at -80 °C until RNA extraction.
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bioRxiv - Cancer Biology 2020Quote: ... samples were stored in RNA later solution (Qiagen) at −20°C and used for lysate preparation ...
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bioRxiv - Immunology 2022Quote: ... 700 μL of a chaotropic agent solution (Qiagen) was added to individual wells and cells were lysed by scraping and vigorous shaking ...
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Ultra-high-throughput microbial single-cell whole genome sequencing for genome-resolved metagenomicsbioRxiv - Microbiology 2022Quote: ... 40 μL Stop Solution (Qiagen, catalog no. 150345), 22 μL N,N-methylenebisacrylamide (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... with the addition of RNase A Solution (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... immersed in RNAlater Stabilization Solution (Qiagen Inc., USA) and stored at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... with the addition of RNase A Solution (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... and ATL buffer from PowerMag Bead Solution (Qiagen) and homogenized using a bead mill homogenizer (Bead Ruptor Elite ...
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bioRxiv - Microbiology 2023Quote: ... 0.025 mL of Proteinase K solution (Qiagen; 19133) was then added to each tube and incubated at room temperature for 10 minutes ...
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bioRxiv - Genomics 2023Quote: ... and resuspended in Qiagen Cell Lysis Solution (Qiagen).
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bioRxiv - Molecular Biology 2023Quote: ... and 10 μL DNase I stock solution (Qiagen) was then added to the column and incubated for 15 mins at RT ...
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bioRxiv - Evolutionary Biology 2024Quote: ... with ceramic beads and ATL lysing solution (Qiagen). DNA was then purified using the KingFisher Flex magnetic particle processor system (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... 0.025 mL of Proteinase K solution (Qiagen; 19133) was then added to each tube and incubated at room temperature for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... and an RNase-free DNase I solution (Qiagen) for extraction of total RNA ...
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bioRxiv - Microbiology 2024Quote: ... then suspended in RNAprotect solution (Qiagen, Hilden, Germany) for 5 mins ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was isolated using miRNeasy solutions (Qiagen, 217004) with Zymo-Spin columns (Zymo Research ...
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bioRxiv - Bioengineering 2024Quote: ... All constructed plasmids used in this study were validated via DNA sequencing and purified to transfection-grade quality using the QIAGEN Plasmid Plus Midi kit (QIAGEN, USA).
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR of the technical replicates was performed using a QuantiFast SYBR Green PCR kit (Qiagen) with the following cycling conditions ...
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bioRxiv - Developmental Biology 2021Quote: Approximately 500bp fragments of axolotl brachyury and sox2 were PCR amplified using OneStep PCR Kit (Qiagen) from RNA extracted from axolotl embryos at various developmental stages using the following primers:
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bioRxiv - Evolutionary Biology 2020Quote: ... Barcoded PCR products were pooled and purified using a QIAquick PCR Purification Kit (Qiagen, Hilden, Germany) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... q-PCR was performed using a Rotor-Gene Q Real-time PCR machine (Qiagen, Hilden, Germany). The oligonucleotides used in PCR were Kdm2b forward ...
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bioRxiv - Developmental Biology 2022Quote: ... CDNA synthesis and qRT-PCR were performed using miRCURY LNA miRNA PCR Starter kit (Qiagen, #339320) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using a MinElute 96 UF PCR Purification Kit (Qiagen, Valencia, CA, USA). Libraries were sequenced (2 x 300 bases ...
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bioRxiv - Genetics 2020Quote: ... Conditions for a 25 μl PCR reaction using the 1 × Taq PCR Master Mix kit (Qiagen) were ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR for microRNA was done following the instructions of miRCURY SYBR Green PCR Kit (Qiagen). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time PCR reactions were set up in triplicates using QuantiFast SYBR Green PCR Kit (Qiagen) and then carried out on the 7500 Real-Time PCR system (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... Amplified PCR products of the selected samples were purified using a QIAquick PCR Purification Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products (250∼280 bp) were purified using a MinElute PCR Purification Kit (QIAGEN, USA) and the libraries were constructed with VAHTS Universal DNA Library Prep Kit for Illumina V3 (Vazyme ...
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bioRxiv - Microbiology 2021Quote: ... both the PCR fragments and the vector were purified with the QIAquick PCR purification kit (Qiagen) and ligated with the T4 DNA ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR amplicons from each reaction were purified using a QIAquick PCR Purification kit (Qiagen, Venlo, Netherlands). In vitro transcription to generate dsRNA for each target gene and the negative control was performed by combining reagents of the T7 RiboMAX™ Express RNAi System (Promega ...
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bioRxiv - Immunology 2022Quote: ... PCR products were purified using a MinElute 96 UF PCR Purification Kit (Qiagen, Valencia, CA, USA). Libraries were sequenced (2 x 300 bases ...
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bioRxiv - Genomics 2022Quote: ... Purified PCR products (400 ng) were mixed with 2 μL 10X Taq polymerase PCR buffer (QIAGEN) and nuclease free water to a final volume of 20 μL ...
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bioRxiv - Cell Biology 2022Quote: ... RT-PCR was performed using the QuantiFast SYBR Green OneStep RT-PCR kit (Qiagen, Manchester, UK). PCR primers were ordered from Invitrogen Ltd and ThermoFisher-Scientific (London ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using a MinElute 96 UF PCR Purification Kit (Qiagen, Valencia, CA, USA). Libraries were sequenced (1 x 300 bases ...
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bioRxiv - Microbiology 2021Quote: ... The PCR mixture contained 1x master mix for the Type-it Microsatellite PCR Kit (Qiagen, France), 3 µL of Q-Solution and 0.25 µM of each primer in a total volume of 30 µL ...
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bioRxiv - Cell Biology 2021Quote: ... 0.6 µM each XBP1 specific primers and one-step RT-PCR (QIAGEN OneStep RT-PCR, 210212). The PCR products were analysed by 2% agarose gel ...
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bioRxiv - Immunology 2020Quote: ... Quantitative PCR was performed using primers listed in Table S3 and QuantiNova SYBR Green PCR (Qiagen) or iTaq™ Universal SYBR® Green Supermix (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... Real-time PCR analysis was further performed on a StepOnePlus Real-Time PCR System from Qiagen using 2x qPCRBIO SyGreen Mix Hi-ROX ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were combined and isolated using the QiaQuick PCR purification kit (Qiagen, Cat. No. 28106). The quality and concentration of the PCR products was determined using a 2200 TapeStation and D1000 screen tapes (Agilent Technologies) ...
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bioRxiv - Genomics 2020Quote: ... The PCR amplification was carried out in Rotor-Gene-Q Real-Time PCR System (Qiagen, USA) with the following program ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Resulting PCR products were then purified using the QIAquick® PCR Purification Kit (Qiagen, Hilden, Germany) before Sanger sequencing by the Sequencing Facility at the Johns Hopkins School of Medicine ...
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bioRxiv - Genomics 2021Quote: ... PCR amplicons were purified using a QIAquick PCR purification kit according to the manufacturer’s instructions (Qiagen) and then they were Sanger sequenced ...
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bioRxiv - Genetics 2020Quote: ... The PCRs were carried-out in 10μl reaction volume following QIAGEN Multiplex PCR Kit (Qiagen, Germany). The thermal cycle profile was ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified using a MinElute 96 UF PCR Purification Kit (Qiagen, Valencia, CA, USA). Libraries were sequenced (2 x 300 bases ...
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bioRxiv - Microbiology 2021Quote: ... RNA was then used for RT-PCR using the QIAGEN OneStep RT-PCR kit (Qiagen, Germany) at 50°C for 30 minutes ...