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Citations for Qiagen :
951 - 1000 of 1056 citations for Urotensin II Related Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... tissues were placed in 300μL of sterile PBS containing sterile glass beads and mechanically lysed at a frequency of 20 shakes per seconds for 3 minutes in a TissueLyser II (Qiagen). Negative controls consisted of tubes containing PBS and beads but no sample ...
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bioRxiv - Cancer Biology 2023Quote: ... NHEJ reporter plasmid was digested with I-Sce1 for 6h and purified using a QIAEX II Gel Extraction Kit (QIAGEN). Exponentially growing cells were transfected using an Amaxa nucleofector with the U-023 program ...
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bioRxiv - Physiology 2024Quote: ... Frozen livers were homogenized in RIPA buffer with protease and phosphatase inhibitors at 30Hz for two minutes in the Tissue Lyser II (Qiagen). Whole tissue lysate was stored at -80°C ...
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bioRxiv - Neuroscience 2024Quote: ... mRNA brain tissue punches were homogenized in 96-well plate format on a QIAGEN TissueLyser II (Qiagen, Valencia, CA; #85300) in 600 μL of homogenizing buffer (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Tissues were ground in tubes using a Qiagen Tissue Lyser II and mRNA was extracted using RNeasy Plant Mini kit (Qiagen) according to manufacture instructions ...
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bioRxiv - Genomics 2024Quote: ... The amplicon library pools were isolated based on size by gel electrophoresis using a 2% agarose gel and then purified using QIAEX II Gel Extraction Kit (QIAGEN) and using 30uL of QIAEX II beads for each sample ...
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bioRxiv - Developmental Biology 2024Quote: ... each snap-frozen TA muscle specimen was placed in 500 μl of CelLyticTM Mammalian Tissue Lysis Reagent supplemented with protease inhibitors and homogenized using a TissueLyser II apparatus from Qiagen to achieve uniform cell disruption ...
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bioRxiv - Cancer Biology 2024Quote: ... Tissue was homogenized in 600 μL Buffer RLT Plus with β-mercaptoethanol (β-ME) using the TissueRuptor II (QIAGEN, 9002755). After centrifugation ...
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bioRxiv - Immunology 2024Quote: ... semimature SP thymocytes were isolated on BD FACSAria II and BD FACSAria Fusion cell sorters directly into RLT + 1% beta-mercaptoethanol (BME) buffer (Qiagen). Libraries were generated by the Emory Integrated Genomics Core (EIGC ...
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bioRxiv - Physiology 2024Quote: ... was mechanically homogenised in TRI reagent with a 5 mm steel bead at 30 Hz for 2 x 30 s (TissueLyser II, Qiagen) then centrifuged for 10 min at 10,000 g ...
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bioRxiv - Microbiology 2020Quote: ... whereas 30 mg of lung tissue was homogenized in 600 μl RLT-buffer containing β–mercaptoethanol for 30 sec at 15 Hz using the TissueLyser II (Qiagen).
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bioRxiv - Evolutionary Biology 2020Quote: ... was ground to small pieces by one steel ball (Ø 4 mm) in a 2 ml Eppendorf tube with Tissue Lyzer II (Qiagen, Hilden ...
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bioRxiv - Neuroscience 2022Quote: ... and the tissue was homogenized with a 5 mm tungsten carbide bead in a bead mill homogenizer (Qiagen, Tissue Lyser II) at 30 Hz for 1.5 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... followed by RNA extraction with TRIzol using Tissue Lyser II (30 Hz frequency for 2 min with 2 cycles, Qiagen, Germany). Equal amounts of RNA from each sample were reverse transcribed in 20 μl to produce cDNA using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Biochemistry 2020Quote: ... embryo pellets were homogenized by cryo-milling after addition of a 2.5 or 5 mm steel bead using a tissue lyser (QIAgen TissueLyser II) at 25 Hz for 15 seconds 3-6 times ...
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bioRxiv - Biochemistry 2020Quote: ... Cell pellets were powderized by cryomilling after addition of a 2.5 mm steel bead using a tissue lyser (QIAgen TissueLyser II) 3 times at 25 Hz for 30 seconds ...
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bioRxiv - Molecular Biology 2022Quote: ... In all samples disruption occurred in 1 min intervals to avoid heat generation derived from the TissueRuptor II (catalogue #9002755, Qiagen, MD)29.
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bioRxiv - Evolutionary Biology 2022Quote: ... 10-20 mg of silica-dried stem or leaf tissue was ground with a Qiagen TissueLyser II (Qiagen, Melbourne, Vic., Australia). DNA was extracted using the DNeasy 96 Plant Kit or DNeasyPlant Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... with 1 min rest on ice in between each round of bashing) on a TissueLyser II beadmill (Qiagen, Valencia, CA, USA), then clarified by centrifugation at 2000 rpm at 4°C for 5 min in a refrigerated tabletop centrifuge (Beckman Coulter ...
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bioRxiv - Genetics 2019Quote: ... The whole body of a single insect was homogenized in a PCR well containing 50 μl of the Lysis & Binding Solution and zirconia beads (ø 2 mm; Nikkato) in TissueLyser II (QIAGEN) at 25 Hz for 30 s ...
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bioRxiv - Systems Biology 2019Quote: ... For improving the lysis step proteinase k and lysozyme were added to the lysis solution and the samples were homogenized using Tissue lyser II (Qiagen S.r.l.). The concentration and purity of RNA were analyzed using a NanoDrop ND-1000 (Thermo Fisher Scientific ...
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rpoB, a promising marker for analyzing the diversity of bacterial communities by amplicon sequencingbioRxiv - Microbiology 2019Quote: ... and were subjected to three cycles of grinding (1 minute, at 30 Hz followed by 1 minute without agitation) in a TissueLyser II apparatus (Qiagen, France). The solutions obtained from crushed nematodes or the contents of G ...
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rpoB, a promising marker for analyzing the diversity of bacterial communities by amplicon sequencingbioRxiv - Microbiology 2019Quote: ... IJs were crushed by three cycles of mechanical grinding (2 minutes at 30 Hz followed by 1 minute without agitation) in a TissueLyser II apparatus (Qiagen, France). We added 2 µL of Ready-Lyse Lysozyme Solution (Epi-centre ...
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bioRxiv - Physiology 2019Quote: ... and tissue pellets were homogenized for 2.5 min at 25 Hz in a mixer mill (Tissue Lyser II, Qiagen, Hilden, Germany). Following this ...
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bioRxiv - Pathology 2021Quote: ... Samples were lysed in 175 μL of RNA lysis buffer added with β-mercaptoethanol and 350 μL of RNA dilution buffer for tissue disruption using a TissueLyser II LTTM (Qiagen). Samples were incubated at 70°C for 3 min ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and DNA was extracted using a TissueLyser II bead mill together with the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) following supplier recommendations.
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bioRxiv - Biochemistry 2019Quote: ... genomic DNA was de-crosslinked in ChIP elution buffer containing 5 M NaCl at 65 °C overnight and purified with the Qiaex II kit (Qiagen, 20021) and eluted in water for PCR amplification ...
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bioRxiv - Microbiology 2019Quote: ... Tissue samples were homogenized with the kit Lysis buffer and 2.8 mm ceramic beads (MO BIO Laboratories Inc., Carlsbad, USA) using TissueLyser II (Qiagen, Hilden, Germany). Subsequently total RNA was extracted as described in the protocol with a DNase I treatment step as per the manufacturer’s instructions ...
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Revisiting a GWAS peak in Arabidopsis thaliana reveals possible confounding by genetic heterogeneitybioRxiv - Genetics 2021Quote: ... Tissue was disrupted by adding two small stainless beads bearings and agitating with a tissue lyser (tissuelyser II, Qiagen, Hilden, Germany) for 10 min at 20 rev/s ...
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bioRxiv - Microbiology 2022Quote: ... PCR products of approximately 440 bp were visualised by gel electrophoresis and gel-purified using the QIAEX II Gel Extraction Kit® (QIAGEN). The purified barcoded amplicons were quantified by Qubit™ dsDNA BR Assay Kit (Life Technologies ...
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bioRxiv - Pathology 2022Quote: ... tissue samples were homogenized in 1 ml of phosphate buffered saline with a metal bead using a TissueLyzer II (Qiagen GmbH). Viral nucleic acids were extracted from blood and homogenized spleen ...
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bioRxiv - Molecular Biology 2023Quote: Isolated RNA samples were reverse-transcribed into cDNA in 20 μl final reaction volumes using miScript II RT Kit(Qiagen,,Germany) as specified in the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Complementary DNA (cDNA) was subsequently generated using 2 μg of total RNA and the miScript II Reverse Transcription (RT) Kit (Qiagen, Australia), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: The fresh-frozen brain tissue from control and epileptic animals was homogenized with a handheld rotor-stator homogenizer (QIAGEN, TissueRuptor II) in a lysis buffer that contains 0.5% Triton X-100 ...
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bioRxiv - Microbiology 2024Quote: ... followed by rotation of the adapter and ten additional minutes of bead beating using a TissueLyser II (QIAGEN; Cat. No. 85300) using a 2 ml Tube Holder Set (QIAGEN ...
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bioRxiv - Microbiology 2024Quote: ... samples were thawed and homogenized at a frequency of 26/s for 1 minute in a TissueLyser II (Qiagen, Germantown, MD) and centrifuged at 16,500xg for 5 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... One mL TRIzol was added to all samples and homogenized with stainless steel grinding balls for 2 minutes at 30 Hz using a TissueLyser II bead mill (Qiagen, USA). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... The homogenization was performed using a Tissue-Lyser II with stainless steel grinding balls (2 x 30s at 30 Hz) (Qiagen, USA). After 30 minutes of end-over-end at 5°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 500ng of RNA were used to perform reverse transcription using the miScript RT II Kit and HiFlex Buffer Reverse Transcription (Qiagen, Netherlands). Quantitative polymerase chain reaction (qPCR ...
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bioRxiv - Physiology 2023Quote: ... 1 µg of RNA isolated from human islets or 10 µL of exosomal RNA was reverse transcribed using the miRScript II kit (Qiagen, Germany), following manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Sand and rhizosphere samples were prepared by adding 250 mg of the collected substrate to a PowerBead plate and homogenized using a TissueLyser II (Qiagen, USA) at a frequency of 20 Hz for 10 minutes ...
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bioRxiv - Genomics 2023Quote: Flash frozen male pupa was homogenized in 650µl of homogenization buffer (10mM Tris-HCL-pH 8.0 and 25mM EDTA) using TissueRuptor II (Qiagen, Germany; Cat # 9002755). 650µl of lysis buffer (10mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... and miRNAs from each HTM cells was reverse transcribed into cDNA using miScript II RT kit as per the manufacturer’s instruction (Qiagen, Hilden, Germany). RT-qPCR was performed in a total volume of 20 µl containing 25 ng of total cDNA ...
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bioRxiv - Physiology 2023Quote: Whole adipose tissue or isolated primary adipocytes and aorta were mechanically homogenised with 5mm stainless steel beads using a TissueLyser II system (Qiagen, UK) in the presence of 700μL QIAzol Lysis Reagent (Qiagen ...
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bioRxiv - Plant Biology 2023Quote: ... leaf-discs were collected and ground in autoclaved Milli-Q water using a TissueLyser II (QIAGEN; 45 seconds at 30 Hz). Serial dilutions of the ground tissue were spotted onto low salt Luria Bertani (LB ...
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bioRxiv - Genetics 2023Quote: ... Further confirmation of which allele was deleted in clones with only one UBE3A copy was performed by utilizing the EpiTect Methyl II DNA Restriction Kit (QIAGEN, #335452) to measure methylation at the PWS-IC (SNRPN ...
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bioRxiv - Microbiology 2023Quote: ... one aliquot was not processed any further and one other aliquot was bead beaten with 100 mg of sterile glass beads (400-600 nm) for 30 s at 30 Hz in a Tissue-lyser II (Qiagen, Germany), in order to open up Symbiodiniaceae cells and release intracellular bacteria to facilitate their growth on agar plates ...
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bioRxiv - Immunology 2023Quote: ... 120 mg l− 1 sodium pyruvate and 1% penicillin–streptomycin) at 300 Hz for 2 minutes using a TissueLyser II (Qiagen, Germany).
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bioRxiv - Biochemistry 2023Quote: ... solution and 150 µL water were added to the samples in Eppendorf tubes which were then grinded three times for 30 seconds using a TissueLyser II (Qiagen®) after addition of metal beads ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... We placed at least 200mg of feces into individual bead tubes from the kit and then homogenized the sample (Tissue Lyser II, Qiagen, Germany) two times for 20 seconds ...