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Citations for Qiagen :
801 - 850 of 1056 citations for Urotensin II Related Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Frozen samples were homogenized with zirconia beads YTZ-4 (AS-ONE, Osaka, Japan) using TissueLyser II (Qiagen, Hilden, Germany), and total RNA was then extracted using the Maxwell 16 LEV Plant RNA Kit (Promega ...
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bioRxiv - Immunology 2021Quote: ... Samples were added to 2 ml ZR BashingBead lysis tube with 0.5 mm beads (Zymoresearch) and tubes arranged in a pre-cooled Tissuelyzer II adapter (Qiagen) and disrupted by shaking at 50 Hz for 25 min ...
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bioRxiv - Genomics 2021Quote: ... Long range PCR amplicons and non-enriched DNA were further purified using the QIAEX II Gel extraction Kit (Qiagen). Due to indispensable loss of HCMV-DNA (>80% ...
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bioRxiv - Genetics 2022Quote: ... snap-frozen kidneys were homogenized and lysed in MagMAX™-96 lysis buffer using a TissueLyser II machine (Qiagen). RNA quality and concentration were determined by a NanoDrop-1000 spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... and then homogenized tissues in 1 ml PBS containing a penicillin/streptomycin mixture by using a TissueLyser II (Qiagen) at 30-Hz oscillation frequency for 3 min ...
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bioRxiv - Genomics 2024Quote: ... after the lysis step (step 4 in handbook protocol) and (ii) adding 700 mL of buffer RWT (Qiagen 1067933) instead of the provided RW1 (step 6 in handbook protocol).
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bioRxiv - Microbiology 2024Quote: ... for two cycles of two minutes each at an oscillation frequency of 30/second using a TissueLyser II (Qiagen). RNA was extracted using the miRNeasy mini kit (Qiagen) ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse thyroid tissue was homogenized in buffer RLT using TissueLyser II (Qiagen; 2x 2 min cycles at 30 Hz) and 5 mm stainless steel beads ...
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bioRxiv - Neuroscience 2024Quote: ... was added to tissue and lysed using a sterile metal bead with rapid agitation in a TissueLyser II (Qiagen). Lysate was then mixed with UltraPure phenol:chloroform:isoamyl alcohol (ThermoFisher ...
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bioRxiv - Plant Biology 2023Quote: ... Leaf discs were ground in 1 mL sterile water with the help of a tissue lyser (QIAGEN, TissueLyser II) using 1.5 mL safe-lock Eppendorf tubs containing three 2.4 mm metal beads ...
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bioRxiv - Biochemistry 2023Quote: ... 5-6 mg of frozen tissue was homogenized in 250 μL ice-cold acidified methanol (0.1% HCl) and 250 μL water washed with hexanes in a bullet blender (TissueLyser II, Qiagen) with 5 mm stainless steel beads (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... 25 mg of freeze-dried leaf tissue was ground into a fine powder using two 1.5 mm diameter steel beads in a TissueLyser II (Qiagen) at 30 Hz for 1 minute ...
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bioRxiv - Genetics 2023Quote: ... leaf tissues were collected and homogenized in 400 μL of TPS buffer (100 mM Tris-HCl, 10 mM EDTA, 1 M KCl, pH8.0) using TissueLyser II (Qiagen), followed by incubation for 20 min at 75°C ...
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bioRxiv - Microbiology 2023Quote: ... and the 9.126 kb backbone was gel purified using a Qiaex II Gel Extraction Kit (Qiagen Cat. No. 20051), alongside the PCR product ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were treated with Angiotensin-II (1 μM) alone or in combination with DAPT (10 μM) or siNotch1 (Qiagen) (50 nM ...
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bioRxiv - Physiology 2023Quote: Livers were powdered in liquid nitrogen then homogenized using GCTU solution (6M guanidinium chloride and 1.5M thiourea) and a hand-held homogenizer (TissueRuptor II, Qiagen). Liver and plasma aliquots were injected into 96-well plates (2.4 mL/well ...
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bioRxiv - Microbiology 2023Quote: ... and seeds (in situ) were ground using two sterile stainless-steel beads in a Tissue Lyzer II (Qiagen, Germany) for 3×30s at 30Hz ...
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bioRxiv - Bioengineering 2023Quote: ... PCR products were extracted from agarose gels after electrophoresis and purified using the QIAEX II Gel Extraction Kits (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... x mg solid matrix were mixed with five times the μl amount of ACN:water (1:1, v/v) and homogenised with a TissueLyser II (30 Hz, 10 min; Retsch Qiagen). After a short centrifugation (2 min ...
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bioRxiv - Biochemistry 2023Quote: ... 50 mg of tissue was homogenized in 500 µl Sucrose Buffer (250 mM sucrose, 20 mM imidazole, pH 7.0) using a TissueLyser II (Qiagen). Tissues from 3-4 mice were pooled together ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse thyroid tissue was homogenized in buffer RLT using TissueLyser II (Qiagen; 2x 2 min cycles at 30 Hz) and 5 mm stainless steel beads ...
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bioRxiv - Molecular Biology 2023Quote: ... The tubes were shaken for 2×45 seconds at 30 Hz using a Tissue Lyser (TissueLyser II, Qiagen, Germany). After homogenization ...
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bioRxiv - Molecular Biology 2023Quote: ... one leaf disc (diameter 0.9 cm) from corresponding inoculated spots of three individual plants were pooled and disrupted using the TissueLyser II (Qiagen) for 30 sec at 30 Hz ...
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bioRxiv - Biophysics 2023Quote: ... The α1(L9’T) and αm mutations were introduced individually or serially by site-specific mutagenesis (QuikChange II, Qiagen). Mutations βm and γm were obtained from GENEWIZTM ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA and RNA from healthy tissues and lymphomas of control and STIL-transgenic mice was extracted using the All Prep DNA/RNA/Protein Mini Kit according to manufacturer’s instructions after tissue homogenization using TissueLyser II and stainless-steel beads (5 mm, all Qiagen). DNA and RNA concentrations were quantified with Qubit 2.0 using the dsDNA High Sensitivity and RNA High Sensitivity Kit (both Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The tissue pellets were resuspended in 1 ml of FSW using a tissue lyser (Tissue-Lyser II, Qiagen, Australia) and the resuspension was homogenized using sterile glass homogenizers for 30 s ...
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bioRxiv - Plant Biology 2024Quote: ... and ground for 1 min at 30 Hz with the pre-cooled Retsch Mill (Tissue Lyser II, Retsch, Qiagen). For quantification of S ...
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bioRxiv - Plant Biology 2024Quote: ... Linear fragments corresponding to the expected sizes were gel purified using the QIAEX II Gel Extraction Kit (Qiagen Corp.) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... for two cycles of two minutes each at an oscillation frequency of 30/second using a TissueLyser II (Qiagen). RNA was extracted using the miRNeasy mini kit (Qiagen) ...
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bioRxiv - Physiology 2021Quote: Lyophilized mouse hearts were homogenized in RIPA buffer supplemented with protease and phosphatase inhibitors (Pierce) using a TissueLyser II apparatus (Qiagen), followed by sonication and centrifugation (10 min at 12,000 rpm at 4°C) ...
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bioRxiv - Microbiology 2020Quote: Oral fluid samples were prepared by homogenization of 200 μl sample (30 sec at 15 Hz) in a Tissuelyser II (Qiagen) followed by centrifugation (2 min at 10.000 rpm) ...
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bioRxiv - Biochemistry 2021Quote: ... Cells with lysis buffer were pulverized in a 10 mL jar containing a 12 mm grinding ball using a TissueLyser II (QIAGEN) by performing 5 rounds of 3 min at 15 Hz ...
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bioRxiv - Microbiology 2021Quote: ... pGRG36 was also digested with XhoI and XmaI and extracted via gel extraction using the QIAEX II gel extraction kit (Qiagen). The digested pGRG36 and cloned chitinase gene were ligated together using ElectroLigase (New England Biolabs ...
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bioRxiv - Genomics 2022Quote: ... A steel bead was added to the tube and homogenized for 30 s at 30 Hz using a Tissue Lyser II (Qiagen). Homogenate was loaded onto a DNA spin column and the flow through supplemented with ethanol was loaded onto an RNA spin column ...
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bioRxiv - Molecular Biology 2020Quote: ... Single alanine substitutions were introduced throughout the α1M2-M3 linker from L276-T283 in addition to the gain of function α1L9’T pore mutation (rat α1L263T) (QuikChange II, Qiagen). Each construct was verified by forward and reverse sequencing of the entire gene ...
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bioRxiv - Cancer Biology 2019Quote: Mouse muscles was pulverized in liquid nitrogen and homogenized 2 × 0.5 minutes at 30 Hz using a TissueLyser II bead mill (Qiagen, USA) in ice-cold homogenization buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... EpiTect II DNA Methylation Enzyme Kit (335452) and EpiTect methyl II PCR system (EPMM104707-1A) for CpG methylation analysis was procured from Qiagen. [γ-32P]ATP was sourced from
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bioRxiv - Microbiology 2019Quote: ... for sample preparation each Nucleospin Tube Type A containing 100 mg of feces was loaded into pre-chilled adapters and shaken twice for 30 sec with a TissueLyser II instrument (Qiagen) at a frequency of 25 Hz ...
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bioRxiv - Systems Biology 2020Quote: ... Cells in cold methanol were transferred to 2 ml polypropylene tubes and metabolite extraction was carried out with a TissueLyser II (Qiagen), using tube holders pre-cooled at -20 °C and adding the same volume of acid-washed glass beads (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: The decellularized Balb/c mouse pancreas scaffolds were washed in PBS and homogenized-lysed for 1 hr in Tissue homozilyser II (Qiagen). The ECM proteins were dissolved in lysis buffer containing Tris HCl 0.06M ...
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bioRxiv - Immunology 2019Quote: ... 6µL ethanol/phosphate buffer (85:15 v/v ethanol/10mM phosphate buffer) were added and the tissue was homogenized (TissueLyser II, Qiagen). Samples were centrifuged at 5000g for 5 minutes at 4°C and the supernatants were transferred to a fresh tube and used for analyses ...
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bioRxiv - Cell Biology 2019Quote: ... Lentivirus carrying the response elements for type I (ISRE - #CLS-008L-1) or type II (GAS - #CLS-009L-1) upstream of firefly luciferase was purchased from Qiagen. Twenty-four hours post plating ...
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bioRxiv - Physiology 2021Quote: ... 5 μL buffer/mg of tissue) to each sample right before mechanical lysis using the TissueLyser II (20 Hz, twice for 90 seconds) (Qiagen). Homogenates were centrifuged at +4°C (600g ...
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bioRxiv - Genetics 2021Quote: RNA was extracted from half of a 4 mm biopsy punch for each region of the brain using the Qiagen TissueLyser II for tissue disruption with 5mm stainless steel beads (Qiagen). The RNeasy Plus kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... was added to each tube and samples were homogenised twice at 25 Hz for 30 sec using a TissueLyser II (85300; QIAGEN) and centrifuged for 2 min at 2,000 x g ...
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bioRxiv - Neuroscience 2021Quote: ... To each of those mixtures 150 μl of ice-cold MeOH was added and subsequently homogenized using a TissueLyser II (Qiagen). To support protein precipitation ...
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bioRxiv - Bioengineering 2021Quote: ... dsDNA product was run on a 2% agarose gel and extracted with the Qiaex II Gel Extraction Kit (Qiagen, 20021). dsDNA was transcribed to RNA using 5 reactions of the Durascribe transcription kit as described above ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized using the miScript II RT Kit with Hiflex buffer from 12 μL of extracted total RNA according to product manual (Qiagen). Expression of mRNA was quantified using QuantiTect SYBR Green PCR kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... samples were thawed and homogenized with a single 3 mm Eliminase washed stainless steel ball bearing in for 3 min at 30 Hz (TissueLyser II, Qiagen) then purified by the Direct-zol RNA microprep kit (Zymo).
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bioRxiv - Plant Biology 2020Quote: Frozen wheat grain samples were freeze-dried then placed in microfuge tubes with stainless steel beads and ground using a TissueLyser II (QIAGEN) for 3 min at a frequency of 30/s ...