Labshake search
Citations for Qiagen :
901 - 950 of 1056 citations for Urotensin II Related Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2021Quote: ... frozen and lyophilised in a 2 ml 96 deep-well plate following which the samples were ground in the TissueLyser II (QIAGEN) using a steel ball in each well for 4-6 minutes at a frequency of 25 Hz ...
-
bioRxiv - Genetics 2019Quote: ... in each well of 8-stripped PCR tubes by shaking with a zirconia bead (2 mm in diameter, Nikkato, Japan) in TissuLyser II (Qiagen) for 30 s at 30 Hz ...
-
bioRxiv - Molecular Biology 2021Quote: ... at 55°C for 8h followed by 20 min heat inactivation at 80°C and gel purified using the QIAEX II Gel Extraction Kit (Qiagen). The gene fragments were ligated into the backbone using T4 DNA ligase (NEB ...
-
bioRxiv - Developmental Biology 2022Quote: Total RNA samples were obtained from dissected tissues from adults or from a pool of embryos or larvae using RNAble solution (Eurobio) and a TissueLyser II system (Qiagen). Total RNA samples were treated with DNAse I (Roche ...
-
bioRxiv - Genetics 2022Quote: ... 8 cm of leaf tissue was harvested on ice and then lyophilized using a TissueLyser II (Qiagen, Valencia, CA, USA). Genomic DNA was extracted using a Qiagen DNeasy Plant Mini Kit (Qiagen ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... mosquitoes were homogenized in 1.7 mL tubes with 50 μL of water and approximately ten 1.0 mm diameter zirconia beads (Thistle Scientific 11079110zx) by shaking at 30 Hz for 2 minutes in a Tissue Lyzer II homogenizer (Qiagen). DNA was extracted using a PureLink Genomic DNA Mini kit (Thermofisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... and 150 μL DMEM supplemented with 2% FBS and homogenized using a TissueLyser II (2 cycles at 30 Hz, 1 min, Qiagen). Homogenates were clarified by centrifugation and frozen until plaque assay titration ...
-
bioRxiv - Plant Biology 2022Quote: ... The tissue was then ground to a fine powder using 3/16 inch (4.76mm) ball bearings in a Tissuelyzer II (Qiagen, Germany) in the presence of dry ice in the pockets around tube holders ...
-
bioRxiv - Plant Biology 2022Quote: ... The lyophilized samples were ground to a fine powder at room temperature using 4.76 mm ball bearings in a Tissuelyzer II (Qiagen, Germany). Following the manual’s instructions ...
-
bioRxiv - Physiology 2023Quote: ... small RNA fragment enriched total RNA isolated from each sample was reverse transcribed into cDNA using a miScript II RT Kit (Qiagen). RT-qPCR was performed using miScriptSYBR Green PCR Kit (Qiagen ...
-
bioRxiv - Plant Biology 2022Quote: Leaf tissue samples were collected and freeze-dried in a deep-well plate and ground in a TissueLyser II (QIAGEN) for 4-6 minutes at a frequency of 25 Hz ...
-
bioRxiv - Physiology 2022Quote: ... They were subsequently organized in metal brackets that were stored at -20°C and homogenized with stainless steel beads at 30 Hz using a TissueLyser II (Qiagen). Samples were lysed twice for one minute ...
-
bioRxiv - Neuroscience 2024Quote: ... with 0.9 x tissue mass of lysis buffer (1x RIPA buffer with 2% SDS and 2x protease inhibitor cocktail) and TissueRuptor II (Qiagen) homogenization ...
-
bioRxiv - Neuroscience 2024Quote: ... 100 mg of frozen brain was taken per mouse sample and lysed with 0.9 x tissue mass of lysis buffer (1x RIPA buffer with 2% SDS and 2x protease inhibitor cocktail) and was homogenised using a TissueRuptor II (Qiagen). Zebrafish larvae were similarly extracted ...
-
bioRxiv - Microbiology 2023Quote: ... tissues were placed in 300μL of sterile PBS containing sterile glass beads and mechanically lysed at a frequency of 20 shakes per seconds for 3 minutes in a TissueLyser II (Qiagen). Negative controls consisted of tubes containing PBS and beads but no sample ...
-
bioRxiv - Plant Biology 2024Quote: ... were harvested at the bolting stage using liquid nitrogen and ground to powder form using tissue lyser (Tissuelyser II, Retsch, Qiagen) at 30 frequency/ s for 2 mins and lyophilised overnight ...
-
bioRxiv - Microbiology 2024Quote: ... mosquitoes were homogenized in 500 μl ice-cold 1X Phosphate Buffered Saline buffer with two ice-cold steel bearing balls (3 mm diameter, LOUDET) using a TissueLyser II (Qiagen) and clarified through centrifugation ...
-
bioRxiv - Microbiology 2024Quote: ... one fecal pellet per procedure was placed in a 2 mL round-bottom tube containing a 0.5 cm diameter stainless steel bead and 500 µL of sterile phosphate-buffered saline (PBS) and agitated at 1/30s using a TissueLyser II (Qiagen) for 60 seconds ...
-
bioRxiv - Neuroscience 2024Quote: ... were added to each tube and samples were homogenized for 2×90s at 20 Hz using a TissueLyser II (Qiagen). Next ...
-
bioRxiv - Microbiology 2024Quote: ... were combined from each individual and minced in a combination of 300 µL of body fluid and 300 µL blood using a TissueLysser II (Qiagen). In addition ...
-
bioRxiv - Microbiology 2024Quote: ... mixed with 1 mL phenol:chloroform:isoamyl alcohol (25:24:1 at pH 8) at 70°C for 12 min and bead beating for 2 min (Tissue Lyser II, Qiagen). The mixture was centrifuged at 4°C for 3 min at maximum speed and the aqueous phase was transferred to a new reaction tube ...
-
bioRxiv - Immunology 2024Quote: ... and brain) homogenates collected from mice at 5 dpi and 5 dpc were generated using the Tissue Lyzer II (Qiagen). Briefly ...
-
bioRxiv - Plant Biology 2023Quote: ... samples were flash-frozen in liquid nitrogen immediately prior to grinding and ground using 3 mm glass beads at 30 Hz for 3 min in a TissueLyser II (Qiagen) to a fine powder ...
-
bioRxiv - Plant Biology 2022Quote: ... frozen in liquid nitrogen, ground to a fine powder (3 mm glass beads added to tissue, ground using tissue lyser (TissueLyser II, QIAGEN), 30/second frequency ...
-
bioRxiv - Microbiology 2023Quote: ... samples were transferred from DNA/RNA Shield – Lysis Tube to a 2 ml eppendorf and cells lysed using a TissueLyser II (Qiagen) at maximum speed (5 repetitions of 1 min with 1 min on ice between each) ...
-
bioRxiv - Plant Biology 2023Quote: Arabidopsis rosettes of 4-week-old plants (Col-0, hos1 and HOS1-GFP/hos1 complementation lines) were finely ground using a TissueLyser II (Qiagen), and proteins were extracted using the IP buffer described in the “Immunoprecipitation assays” (above ...
-
bioRxiv - Microbiology 2022Quote: ... transferred to 2 ml lysis matrix B tubes (MPBio) and subjected to bead beating for 15 min at 30 Hz (Tissuelyser II, Qiagen) with RNAse A added ...
-
bioRxiv - Microbiology 2023Quote: ... fresh 0.35 g/L proteinase K) during two rounds of 3 minutes at a 30Hz/s frequency in a TissueLyser II grinder (Qiagen). Total RNA was converted into complementary DNA (cDNA ...
-
bioRxiv - Plant Biology 2023Quote: 8-day-old seedlings growing on agar plates in long-day condition were collected and flash-frozen in liquid nitrogen before powdering with metal beads using a TissueLyser II (Qiagen). Total RNA was isolated using the NucleoSpin® RNA Kit (Thermo ...
-
bioRxiv - Cancer Biology 2023Quote: ... was reverse transcribed into cDNA by miScript RT II Kit using miScript HiSpec Buffer or miRCURY LNA RT Kit (Qiagen). Expression levels of miR-142-5p were analyzed using miScript PCR System or miRCURY LNA SYBR Green PCR Kit (Qiagen) ...
-
bioRxiv - Genetics 2023Quote: ... liver samples were homogenized in Qiazol lysis buffer in a TissueLyser II and RNA isolated with RNeasy Mini Kit (Qiagen) following manufacturer’s protocols ...
-
bioRxiv - Plant Biology 2023Quote: ... Arabidopsis thaliana frozen leaf tissues were weighed and ground by using two 3 mm stainless steel beads for 3 minutes at 30 Hz with frozen adapters on a TissueLyser II (Qiagen). The resulting frozen powder was dissolved in 650 µL chloroform-methanol (3:7 ...
-
bioRxiv - Plant Biology 2023Quote: Frozen tissue was ground into a fine powder using a ⅛” steel ball bearing with 1 min shaking at 25 Hz in a TissueLyser II (Qiagen). Total RNA was extracted from finely ground tissue using TRI reagent (Sigma-Aldrich ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Prior to RNA extraction individuals were homogenized in 200 μl homogenization buffer including 4 μl 1-Thioglycerol using a TissueLyser II (Qiagen) with a mixture of five 1 mm zirconia (BioSpec Products ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... liver tissue was minced to small slices on a clean and cold surface and finally homogenized with the TissueRuptor II device (Qiagen) making use of its maximal settings ...
-
bioRxiv - Genomics 2023Quote: ... Swabs were incubated at 37 degrees Celsius for 30 minutes then bead beaten for 6 minutes at 30Hz in a TissueLyser II (Qiagen). The rest of the protocol was followed increasing the volumes to account for the additional lysis buffer ...
-
bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS and 1x antibiotic-antimycotic by a TissueLyser II bead mill with 5-mm stainless steel beads (Qiagen). After brief centrifugation ...
-
bioRxiv - Plant Biology 2023Quote: ... leaf and kernel tissue was added to prechilled tubes and disrupted using the Tissuelyser II (Qiagen N.V., Venlo, The Netherlands) for 2 rounds at 30Hz for 1 minute each round ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The organs were weighed and lysed in 1 mL Triton X-100 solution (0.1% in PBS) using a TissueLyser II (QIAGEN, Germany) according to the manufacturer protocol ...
-
bioRxiv - Bioengineering 2023Quote: Reverse transcription of isolated miRNAs for quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was performed with a miScript II RT Kit (Qiagen) for selected miRNA targets ...
-
bioRxiv - Molecular Biology 2023Quote: ... and class II T7 promoter (transcripts starting with +1A or NAD) were produced by PCR and purified by PCR purification kit (Qiagen) by manufacturer’s instructions.
-
bioRxiv - Microbiology 2023Quote: Genomic DNA was prepared from 500 µl overnight cultures using the peqGold bacterial DNA mini kit following the instruction of the manufacturer with the modification of physically opening cells with the TissueLyser II (Qiagen). Purified genomic DNA was paired end sequenced (2 x 150 bp ...
-
bioRxiv - Plant Biology 2023Quote: ... 2 h after the light is on) and flash-frozen in liquid nitrogen before powdering with glass beads using a TissueLyser II (Qiagen).
-
bioRxiv - Physiology 2023Quote: ... Approximately 25 mg of powdered gastrocnemius were homogenized with pre-chilled metal beads using a TissueLyser II (Qiagen, Hilden, Germany) for 40 seconds at 25 Hz ...
-
Sweetwater: an underrated crude glycerol for sustainable lipid production in non-conventional yeastsbioRxiv - Systems Biology 2023Quote: ... tubes were vortexed for 15 s and submitted to cell disruption for 15-min at 4°C using a TissueLyser II (Qiagen) at 30 Hz ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and tissuelysed in 100ul of PBS with about ten 1.0mm diameter zirconia/silica beads (Thistle Scientific # 11079110z) using Qiagen TissueLyser II (Qiagen # 85300) for 2 minutes at 30Hz ...
-
bioRxiv - Cancer Biology 2023Quote: NHEJ and HR reporter constructs 38 were digested with NheI restriction enzyme and purified with the QIAEX II gel extraction kit (QIAGEN). The same plasmid DNA preparation was used for generating all reporter cell lines of the studied species ...
-
bioRxiv - Developmental Biology 2023Quote: ... The RNA was extracted using the NucleoSpin RNA II kit (Machery & Nagel) and the cDNA were synthesized using the QuantiTect Reverse Transcription Kit (Qiagen). cDNA samples were run in triplicates and experiments were repeated with independently isolated RNA samples ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Approximately 50 mg of tissue was homogenised in 500 µl phosphate-buffered saline (PBS) for 2 min at 30 Hz using 5 mm steel beads on a TissueLyser II instrument (Qiagen). Total nucleic acids were extracted using the Nucleo Mag Vet Kit (Macherey & Nagel ...
-
bioRxiv - Cancer Biology 2023Quote: ... NHEJ reporter plasmid was digested with I-Sce1 for 6h and purified using a QIAEX II Gel Extraction Kit (QIAGEN). Exponentially growing cells were transfected using an Amaxa nucleofector with the U-023 program ...