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Citations for Qiagen :
51 - 100 of 1575 citations for Recombinant Human Interleukin 3 Receptor Alpha Low Affinity His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... the solubilized His-tagged histones were purified using Ni-NTA beads (Qiagen). For untagged H2B ...
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bioRxiv - Biochemistry 2023Quote: ... and the His-tagged proteins were purified with HisBind NiNTA-agarose (Qiagen) according to the recommendations of the supplier ...
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bioRxiv - Biophysics 2022Quote: ... Protein was purified through affinity chromatography (Ni-NTA His-Bind resin, Qiagen), followed by gel filtration chromatography (Superdex 200 Increase 10/300 GL ...
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bioRxiv - Plant Biology 2023Quote: ... The His-ZmTPL2N-His recombinant protein was purified using Pierce Ni-NTA resin (QIAGEN, Cat. No. 30210) according to the product protocol ...
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bioRxiv - Microbiology 2022Quote: ... recombinant protein was initially purified by nickel affinity chromatography (Qiagen, Holland) and subsequent size exclusion using Superdex 200 increase (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged proteins were purified on Ni-NTA agarose (Qiagen; catalog no. 30761) and GST-tagged TopBP1 protein was purified on Glutathione Sepharose™ 4B (GE healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... The His-tagged TEV protease was removed by incubation with Ni-NTA (Qiagen) overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged fusion proteins were purified with a Ni-NTA column (Qiagen), and the elution fraction was incubated with TEV protease for 15-18 h at 4°C to cleave the N-terminal His-tag ...
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bioRxiv - Immunology 2024Quote: ... His-tagged SLFN11 (residues 349-901) was purified by Ni-NTA beads (Qiagen). All purified GST- or His-tagged proteins were dialyzed against a buffer containing 50 mM Tris-HCl and 150 nM NaCl and validated by Coomassie blue-stained SDSLJPAGE.
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bioRxiv - Neuroscience 2024Quote: ... The His-tagged nanobodies were purified by using Ni-NTA purification column (Qiagen) followed by desalting step using disposable PD-10 desalting columns (Cytiva ...
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bioRxiv - Bioengineering 2024Quote: ... Each His-tagged protein in media was captured with Ni-NTA resin (Qiagen) and eluted with DPBS containing 150 mM imidazole.
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bioRxiv - Plant Biology 2022Quote: ... Target protein was recovered and purified with a His-Trap affinity column (QIAGEN) according to manufacturers’ protocols ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The concentration of the resulting His-tagged ß-CATENIN-1 fragments was quantified via quantitative Western blotting with a Penta-His antibody (Qiagen) and a recombinant His-tagged protein as external standard.
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bioRxiv - Microbiology 2022Quote: ... the recombinant protein was purified by Ni-NTA affinity chromatography column (QIAGEN).
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bioRxiv - Immunology 2020Quote: ... The His-tagged fusion peptide was purified using nickel–nitrilotriacetic acid (Ni–NTA, Qiagen) resin affinity chromatography and by C18 reverse-phase chromatography (Sep-Pak® Waters ...
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bioRxiv - Plant Biology 2020Quote: ... His-tagged proteins were incubated with Nickel-nitrilotriacetic acid (NTA) sepharose (Qiagen, Hilden, Germany) at 4°C with slight shaking for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... N-terminal His-tagged proteins were purified using a QIAexpress protein purification system (Qiagen), as previously described47.
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bioRxiv - Evolutionary Biology 2021Quote: ... His-tagged proteins from the supernatant were incubated overnight with Ni-NTA agarose (Qiagen) at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... coli and purified by His-tag affinity purification using Ni-NTA agarose beads (Qiagen) followed by ion exchange purification using a mono-Q HR 5/5 column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2019Quote: ... His6- and GST-tagged proteins were affinity-purified in batch on Ni-NTA agarose (Qiagen) and glutathione Sepharose 4 Fast Flow (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... His6-tagged AC was secreted into the medium and purified by nickel affinity chromatography (Qiagen) with elution in 100 mM citrate pH 4.0.
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bioRxiv - Plant Biology 2020Quote: ... Recombinant proteins were affinity purified using nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen) and elution with imidazole ...
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bioRxiv - Microbiology 2021Quote: Recombinant protein was blotted with anti-His antibody using the same method: 1:2000 mouse anti-tetra-His IgG (Qiagen); 1:1500 goat anti-mouse IgG-HRP (Dako) ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged CSF3R protein was enriched by nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen 30210) and further purified by size-exclusion chromatography on a Superdex 75 10/300 GL column (Cytiva 17517401 ...
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bioRxiv - Microbiology 2023Quote: ... The His-tagged proteins were purified using the QIAexpress Ni-NTA Protein Purification System (Qiagen) with 0.1 M ...
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bioRxiv - Cell Biology 2020Quote: ... Poly-histidine-tagged recombinant proteins were purified using Ni-NTA-Agarose (Qiagen, Hilden, Germany) beads according to manufacturer’s instructions and were analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Microbiology 2022Quote: ... BapR-CPD-His was affinity purified from cleared lysates using Ni-NTA agarose beads (Qiagen) with gentle rocking at 4°C for 2 hours ...
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bioRxiv - Microbiology 2024Quote: ... His-tag-affinity chromatography-purifcation was performed with a Ni-NTA agarose (Qiagen, Hilden, Germany) gravity flow column ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tagged protein was purified by affinity chromatography on Ni-NTA agarose beads (Qiagen 30230) and cleaved on beads with TEV protease o/n at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... the resolubilized histidine-tagged histones were purified with Ni affinity chromatography using Ni-NTA beads (Qiagen). For untagged H2B ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged BASP1 derivatives were prepared using Ni-NTA beads following the manufacturers instructions (Qiagen). Purified proteins were dialysed into Buffer D and stored at −80°C.
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bioRxiv - Immunology 2022Quote: ... and His-tagged Fabs were isolated from cell supernatants using Ni-NTA columns (Qiagen, Hilden, Germany). IgGs ...
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bioRxiv - Plant Biology 2020Quote: ... The expressed protein was purified as His-tagged fusion protein using Ni-NTA agarose beads (Qiagen) and analyzed by SDS-PAGE.
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bioRxiv - Neuroscience 2020Quote: ... The His-tagged target protein was purified by Ni-NTA gravity-flow chromatography (Qiagen, Hilden, Germany). The eluted protein was subjected to MonoQ 10/100 GL (GE Healthcare Lifesciences ...
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bioRxiv - Microbiology 2020Quote: ... BipA-His was purified from the soluble fraction by affinity chromatography using Ni-NTA agarose (QIAGEN) previously equilibrated with equilibration buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 6x-His-Smt3 was purified from BL21 E.coli cells using a Ni-NTA affinity column (Qiagen) following manufacturer indications ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant Lsr2 proteins were purified using immobilized-metal affinity chromatography (Ni-NTA agarose, Qiagen) and size exclusion chromatography ...
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bioRxiv - Biophysics 2021Quote: ... Media containing his-tagged Ecads was passed through a chromatography column containing Ni-NTA agarose beads (Qiagen). Beads were then washed with a pH 7.5 biotinylation buffer (25mM HEPES ...
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bioRxiv - Synthetic Biology 2021Quote: ... and His-tagged protein was purified from cell lysate using a Ni-NTA spin column (Qiagen, 31014). The resulting product was run on a 12% Bis-Tris PAGE gel (Thermofisher ...
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bioRxiv - Biophysics 2022Quote: ... Media containing his-tagged Ecads was passed through a chromatography column containing Ni-NTA agarose beads (Qiagen). Beads were then washed with a pH 7.5 biotinylation buffer (25 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged Sic1PY conjugates (polyubiquitylated Sic1PY, Ubn-Sic1PY) were purified by incubating with Ni-NTA resin (Qiagen) at 4 °C for 1 h ...
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bioRxiv - Immunology 2020Quote: ... HIS-tagged DR protein monomers were produced by Hi5 insect cells and purified using Ni-NTA (Qiagen) and size exclusion columns (AKTA ...
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bioRxiv - Immunology 2023Quote: ... supernatants were harvested and His-tagged tIgM-Fc (tFcµ) protein was purified using Ni-NTA Agarose (Qiagen) and subsequently ...
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bioRxiv - Bioengineering 2023Quote: ... Purification of His-tagged GA-MatryoshCaMP6s was performed with Ni-NTA agarose beads (Qiagen, cat. No: 30210). Before applying the lysate ...
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bioRxiv - Synthetic Biology 2021Quote: 6His-tagged Mdb1 recombinant protein was expressed in BL21 and purified using Ni-NTA beads (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Recombinant-construct with N-terminal His-tag was purified through IMAC-based Ni-NTA (Qiagen). IPTG induced ...
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bioRxiv - Cell Biology 2024Quote: ... a second transfection of a 0.6 μg plasmid encoding either the C-terminal GFP11 tagged M2tail(368-466) or M2 receptor was performed using the Effectene Transfection Reagent (Qiagen). After washing each well with 2 mL PBS ...
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bioRxiv - Microbiology 2020Quote: ... The 6 × his-tagged proteins were purified using a nickel-nitrilotriacetic acid (Ni-NTA) agarose matrix (30250, Qiagen). Cell-free extracts were loaded on 0.5 ml Ni-NTA matrixes and incubated on a roller shaker for 2 h at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... His-tagged ubiquitin-conjugated proteins were purified by adding 100 µL of 50 % Ni-NTA agarose beads (Qiagen) equilibrated in A2 buffer to 1 mL of cleared extracts and incubated with rotation for 2-4 hr at room temperature ...
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bioRxiv - Microbiology 2020Quote: A 6.5 nM concentration of His-tagged 16055 SOSIP.v8.3 trimer in TBS was added to a 96-well Ni-NTA plate (Qiagen) for 2 h at RT ...