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Citations for Qiagen :
401 - 450 of 1575 citations for Recombinant Human Interleukin 3 Receptor Alpha Low Affinity His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... which was applied to a Ni-NTA affinity chromatography column (Qiagen, Hilden, Germany), equilibrated with 20 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was passed through the equilibrated Ni-NTA-agarose affinity resin (Qiagen). The resin was washed with wash buffer (lysis buffer with 40 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... at 15 °C and purified by affinity chromatography on Ni-nitrilotriacetate resin (Qiagen) followed by TEV protease treatment to remove the tag ...
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bioRxiv - Biochemistry 2019Quote: ... The cleaved tag and protease were removed by Ni-NTA affinity chromatography (Qiagen) and CLOCK PAS-AB was further purified by Superdex75 gel filtration chromatography (GE Healthcare ...
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bioRxiv - Synthetic Biology 2020Quote: ... The soluble fraction was purified by affinity chromatography over Ni-NTA Agarose (Qiagen) gravity columns ...
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bioRxiv - Microbiology 2020Quote: ... Secreted ACE2 were purified from culture medium by Ni-NTA affinity chromatography (Qiagen). Filtered media was mixed 3:1 (v/v ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was isolated by Ni2+-nitrilotriacetic acid (Ni-NTA) affinity chromatography (Qiagen). Proteins were eluted with 50 mM Tris buffer pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a pre-equilibrated NiNTA superflow affinity column (Qiagen). The column was washed with 50mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified by metal affinity chromatography on a Ni2+-NTA column (Qiagen). Cell free extract was loaded onto column followed by two wash steps with wash buffer (Tris 50 mM ...
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bioRxiv - Biochemistry 2021Quote: ... and the soluble fractions were purified using nickel affinity chromatography (Qiagen, Manchester, UK) and eluted with lysis buffer containing 300 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... The RBD protein was purified by affinity chromatography using Ni-NTA resin (QIAGEN), followed by size exclusion chromatography on a HiLoad Superdex 200 pg column (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... and purified by metal chelate affinity chromatography on Ni-NTA agarose (Qiagen, USA), followed by cation exchange chromatography and gel filtration ...
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bioRxiv - Cancer Biology 2022Quote: ... and the supernatants were purified using a nickel-NTA affinity column (Qiagen 30210). Centrifugal filters with molecular 10 kDa molecular weight cutoffs (Millipore UFC801024 ...
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bioRxiv - Microbiology 2022Quote: ... Clarified lysate was passed over a Ni2+ affinity column (Ni-NTA Superflow, Qiagen) and eluted in a buffer with 250 mM imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified supernatant was subjected to affinity chromatography on Ni-NTA resin (Qiagen), and eluted protein was rebuffered using PD 10 columns (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were purified through metal-affinity chromatography using Ni2+–NTA agarose beads (Qiagen). Purified protein aliquots were stored at -30°C in 50 mM Tris-Cl ...
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bioRxiv - Biophysics 2023Quote: ... coli and purified using a combination of Ni-NTA affinity (Qiagen, Hilden, Germany) and size exclusion chromatography on a Superdex 200 column (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: ... an immobilized metal affinity chromatography (IMAC) column packed with Ni-NTA beads (Qiagen) was used for the second time where the flow-through fraction was passed 5 times through the column so that all His-tag labeled molecules were retained ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were purified by immobilized metal affinity chromatography using Ni–NTA agarose (Qiagen) pre-equilibrated with lysis buffer in individual Econo-Pac gravity-flow columns (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was purified through affinity column chromatography using Ni2+/NTA beads (Qiagen). The column was equilibrated with lysis buffer ...
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bioRxiv - Immunology 2023Quote: ... CD89 was purified from the cell supernatant using Ni-NTA affinity resin (Qiagen). Superose 6 SEC was used to further purify CD89 ...
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bioRxiv - Microbiology 2024Quote: ... The fusion protein was affinity purified using Ni-NTA agarose (Qiagen; Table S4), eluted with 50 mM Tris-base ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... and 1000 U DNase I) and purified by nickel affinity chromatography (Qiagen, 30210). Protein were first loaded on HeparinHP column (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transiently transfected with HA-tagged RILP or ORP1L constructs using Effectine (Qiagen), harvested at 24 hours post-transfection and fixed in 3.7% formaldehyde in PBS for 15-20 min ...
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bioRxiv - Microbiology 2020Quote: ... His6-tagged Lem27 or its truncation mutants was purified using Ni2+-NTA columns (Qiagen). After washing with a buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... E2D2/3 (5’-AACAGUAAUGGCAGCAUUUGU-3’) and E2D4 siRNAs (5’ – CCGAAUGACAGUCCUUACCAA-3’) all from Qiagen, USA(83).
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bioRxiv - Microbiology 2021Quote: ... Low molecular DNA was eluted with 50 uL EB buffer (Qiagen).
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bioRxiv - Developmental Biology 2020Quote: ... recombinant proteins were purified using Ni-NTA Agarose (Qiagen 30210). Buffer was exchanged by dialysis to PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant proteins were then purified by Ni-NTA-agarose (Qiagen) affinity chromatography ...
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bioRxiv - Cancer Biology 2023Quote: ... HGF (5’-CTCACACCCGCTGGGAGTAC-3’, 5’-TCCTTGACCTTGGATGCATTC-3’), STAT3 (5’-CTTTGAGACCGAGGTGTATCACC-3’, 5’-GGTCAGCATGTTGTACCACAGG-3’) and B-Actin Primer Set (Qiagen, QT00095431). After preparing master mixes samples were prepared in quadruplicate in a 96-well Reaction Microplates (Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... the fusion protein was purified using His-Bind columns (Qiagen, Venlo, The Netherlands) and analyzed by SDS-PAGE using gels containing 12% polyacrylamide ...
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bioRxiv - Microbiology 2020Quote: ... Western blot analysis was performed using the Penta His HRP conjugate kit (Qiagen). To check for equal loading ...
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bioRxiv - Microbiology 2021Quote: ... His-N-WASP was first isolated using Ni-NTA beads (Qiagen, Valencia, CA) using a buffer with an imidazole gradient (20 mM Bis-Tris ...
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bioRxiv - Microbiology 2021Quote: ... a penta-His HRP conjugate was used (1:4000, QIAGEN GmbH, Hilden, Germany). The detection of GFP was performed with the primary antibody anti-GFP (1:10000 ...
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bioRxiv - Biophysics 2019Quote: ... Anti-tetra-His-tag mouse monoclonal antibody (mAb) IgG1 was purchased from Qiagen Com ...
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bioRxiv - Biochemistry 2021Quote: Assay mixes consisted of 25 nM ALEXA488-conjugated penta-His antibody (Qiagen # 35310), 50 μM DTT ...
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bioRxiv - Microbiology 2022Quote: ... His-Hcp was purified from the soluble fraction by NTA-resin chromatography (Qiagen). Purified protein was desalted with a PD-10 desalting column (GE Healthcare ...
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bioRxiv - Cancer Biology 2024Quote: rEDA was produced intracellularly using the pQE-30 His tag purification system (Qiagen) in E ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were purified by immobilized metal affinity chromatography according to the manufacturer’s protocol (Qiagen), followed by gel filtration in 50 mM sodium citrate buffer (pH 5.5 ...
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bioRxiv - Biophysics 2019Quote: ... The supernatant was loaded onto a Ni-NTA agarose affinity column (Qiagen, Hilden, Germany), washed with ~30 mL of 20 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... then clarified lysates were loaded onto a Ni2+ affinity column (Ni-NTA Superflow; Qiagen), washed in buffer A plus 300 mM NaCl and 20 mM imidazole pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The target proteins were purified by affinity chromatography using a Ni-NTA column (Qiagen) with the lysis buffer supplemented with 20 mM and 200 mM imidazole serving as the washing buffer and elution buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was purified by affinity chromatography using Ni-NTA agarose (Qiagen, Hilden, Germany), followed by gel filtration chromatography ...
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bioRxiv - Biochemistry 2020Quote: ... then clarified lysates were loaded onto a Ni2+ affinity column (Ni-NTA Superflow; Qiagen), washed in buffer A plus 300 mM NaCl and 20 mM imidazole pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... were purified by two rounds of nickel affinity chromatography using Ni-NTA agarose (QIAGEN), with overnight cleavage by N-terminally His-tagged TEV protease (expressed in our laboratory ...
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Large-scale conformational changes of FhaC provide insights into the two-partner secretion mechanismbioRxiv - Biophysics 2022Quote: ... Fha44 was affinity-purified from the supernatants with Ni-NTA beads (Qiagen, Courtaboeuf, France). The membrane extracts were prepared and FhaC was detected as above ...
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bioRxiv - Biochemistry 2021Quote: ... After centrifugation the supernatant was loaded onto a Ni-NTA superflow affinity column (Qiagen), pre-equilibrated on 50 mM Tris-HCl ...
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bioRxiv - Biophysics 2019Quote: ... Proteins were purified by immobilized metal affinity chromatography according to the manufacturer’s protocol (Qiagen), followed by gel filtration in 10 mM sodium phosphate buffer (pH 7.4 ...
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bioRxiv - Microbiology 2019Quote: ... and purified using immobilized metal affinity chromatography using Ni-NTA resin (Qiagen, Hilden, Germany). Eluted proteins were renatured by three-step dialysis to a final buffer composition of 20 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... Supernatant was run by gravity over a column of 6ml NiNTA affinity resin (Qiagen) pre-equilibrated in binding buffer ...