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Citations for Qiagen :
51 - 100 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: The qRT-PCR assays were performed with QuantiNova SYBR Green RT-PCR Kit (Qiagen) using the Applied Biosystems Real-Time PCR System ...
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bioRxiv - Cancer Biology 2022Quote: ... according to manufacturer’s protocol (MiScript Primer assays and II RT kit for cDNA synthesis and MiScript SYBR Green PCR Kit for RT-qPCR, 218161, Qiagen) from which the recovery of cel-miR-39 spike-in control was confirmed.
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bioRxiv - Microbiology 2022Quote: ... This was accomplished using silica membrane spin columns (DNeasy PowerClean Pro Cleanup Kit; Qiagen 12997-50), before elution in 10 mM Tris-Cl buffer (pH 8.5) ...
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bioRxiv - Bioengineering 2024Quote: ... RNA was lysed directly from the PDMS membrane using the RNeasy Mini Kit (Qiagen, Hilden, Germany). RNA concentration and purity were evaluated using a NanoDrop OneC (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA were labeled by nick translation to generate green fluorescent probes using Atto488 Nick Translation Labeling Kit (Jenabioscience) and purified by silica-gel membrane adsorption (Qiagen PCR Purification Kit), according to the kit protocols ...
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bioRxiv - Cell Biology 2021Quote: ... and plasma membrane proteins were purified from cultured astrocytes using a commercially available kit (Qproteome Cell Compartment Kit; Qiagen, Inc, Germantown, MD, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: Clones obtained from three independent conjugation assays were collected and subjected to genomic extraction using the kit DNeasy® Tissue Kit (Qiagen). DNA was mechanically fragmented using the Covaris method (DNA shearing with sonication) ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA concentration was then quantified via Qubit Fluorometer using the RNA BR Assay Kit (Qiagen, ThermoFisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... Plasmid DNA for retrotransposition assays was obtained using a Plasmid Maxi Kit (Qiagen, Cat#: 12163). Each construct was built with and without a cytomegalovirus promoter (CMVp ...
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bioRxiv - Biochemistry 2021Quote: Amplicons from the 720-mer assay were purified via the QIAquick PCR Purification kit (Qiagen). Concentrations were measured using a Qubit® fluorometer (ThermoFisher ...
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bioRxiv - Molecular Biology 2020Quote: Both singleplex and multiplex endpoint PCR assays were based on the Multiplex PCR Kit (Qiagen) and contained bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2024Quote: ... the assay was performed using QuantiFast®Pathogen RT-PCR+IC Kit (Qiagen, Heidelberg, Germany) and carried out on Rotor-Gene Q thermal cycler ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated using a silica membrane RNA purification method (RNeasy Mini Kit, Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted from these membranes using the DNeasy PowerWater kit (cat# 14900-100-NF, Qiagen, USA), using the protocols provided by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... prior to growth inhibition assays and genomic DNA (gDNA) extraction (DNeasy Blood and Tissue kit (Qiagen).
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bioRxiv - Microbiology 2024Quote: ... the RT-PCR assay was conducted utilizing either the QuantiTect Probe RT-PCR Kit (Qiagen, Germany) in strict adherence to the instructions provided by the manufacturers ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasma extracellular vesicles (EVs) were purified by a membrane-affinity approach using an exoRNeasy Midi Kit (Qiagen, 77144) following the manufacturer’s instructions [42] ...
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bioRxiv - Microbiology 2023Quote: ... DNA from viral fraction (142 mm diameter membranes) was extracted using the DNAeasy PowerMax Soil kit (Qiagen, Sweden) and a subsequent ethanol precipitation step for concentrating DNA ...
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bioRxiv - Genomics 2022Quote: ... Assays were designed using PyroMark Assay Design SW 2.0 (Qiagen) and provided in Table S9 ...
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bioRxiv - Microbiology 2021Quote: ... Membrane filters were stored at −80°C until DNA could be extracted using a DNeasy Power Water kit (Qiagen) and bacteria were quantified using qPCR of the vvhA gene.
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bioRxiv - Bioengineering 2024Quote: ... RNA was extracted directly from the bioreactor membranes following manufacturer’s instructions from the RNeasy Mini Kit (Qiagen, Hilden, Germany). RNA concentration was assessed using the Qubit Fluorometer (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... The assay was performed using qRT-PCR and the QuantiNova Probe RT-PCR kit (Qiagen, Düsseldorf, Germany), and the LightCycler 480 Real-Time PCR System (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... Gene expression levels were determined by Sybr green assays (Mm_Trim72_1_SG QT00315959) using QuantiFast SYBR Green PCR Kit (Qiagen). 18S transcript was used as an internal control to normalize the variations for RNA amounts (Mm_Rn18S_3_SG QT02448075) ...
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bioRxiv - Neuroscience 2021Quote: ... all libraries were quantified using the highly sensitive fluorescent dye-based Qubit ds DNA Assay Kit (Qiagen). Whole RNA library preparation was performed using the TruSeq stranded mRNA HT technology (Illumina ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Quantification of the prepared library was carried out using QIAseq Library Quant Assay Kit (Qiagen N.V., Germany). The library was sequenced using NextSeq550 (Illumina Inc. ...
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bioRxiv - Plant Biology 2023Quote: ... Real-time quantitative PCR (qPCR) assays were performed in duplicate using RotorGene SYBR green PCR kit (Qiagen) on Rotor-Gene Q ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA was extracted from surviving colonies of clonogenicity assay using QIAamp UCP DNA Micro Kit (QIAGEN) by following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: Pyrosequencing assays were designed using PyroMark Assay Design 2.0 software (Qiagen). 500 ng genomic DNA was bisulphite-converted with the EZ DNA Methylation-Direct kit (Zymo Research ...
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bioRxiv - Immunology 2022Quote: ... mRNA was extracted from purified T-lymphocytes by silica-membrane spin columns through use of RNAeasy mini kit (#74104, Qiagen), then immediately converted to cDNA by High-Capacity cDNA Reverse Transcription Kit (#4374966 ...
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bioRxiv - Microbiology 2021Quote: DNA extraction from all the 0.22 µm filter membranes was performed using the Power Water DNA isolation kit (Qiagen, USA) according to the manufacturer’s protocol with minor modifications ...
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bioRxiv - Microbiology 2021Quote: ... transwell membranes were excised and submerged in RLT buffer to extract RNA using the RNAeasy kit (Qiagen, cat no. 74104). cDNA synthesis was performed using SuperScript™ III system (ThermoFisher cat no ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from three individual 0.1 µm bulk water filtration filter membranes using the DNeasy PowerSoil DNA extraction Kit (Qiagen, JVWP14225) according to the manufacturer’s instructions and was sequenced as part the Census of Deep Life phase 13 sequencing grant using Illumina NextSeq (paired end ...
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bioRxiv - Molecular Biology 2023Quote: ... cf-DNA quantified from various plasma or serum types using either MitoQuicLy or a silica-based membrane column DNA extraction kits (DNeasy, Qiagen). A total of 50 samples were analyzed (5 biofluids ...
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bioRxiv - Microbiology 2023Quote: Total DNA was extracted from frozen filtered membrane or sediment samples using the DNeasy PowerSoil Pro Kit (Qiagen, Hilden, Germany), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Assays were run in a 384-well format using the QuantiFast Pathogen RT-PCR + IC Kit (QIAGEN; 211454). USA/WA-1/2020 SARS-CoV-2 RNA (20,000 genome copies per reaction ...
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bioRxiv - Microbiology 2020Quote: The quantitative real-time TaqMan based assay was carried out using a One-step RT-PCR kit (Qiagen) in the Light Cycler 2.0 system (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... QuantiTect Primer Assays (Qiagen) or primers designed with the Primer3 software (http://primer3.ut.ee ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: QuantiTect Primer Assays (Qiagen) were used for all the genes analysed ...
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bioRxiv - Immunology 2023Quote: ... QuantiTect Primer Assay (Qiagen) against 18S ribosomal RNA (QT00199367) ...
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bioRxiv - Immunology 2024Quote: ... Quantitect Primer Assays (Qiagen) were used for detection of ARID5A (#QT00049672) ...
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bioRxiv - Immunology 2024Quote: ... QuantiTect Primer Assays (Qiagen) were used to amplify IFI44L (QT00051457) ...
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bioRxiv - Microbiology 2020Quote: ... DNA from the 0.1 μm and pooled 0.2 μm membrane filters with iron flocculates was extracted using PowerMax Soil DNA Extraction Kit (Qiagen, Hilden, Germany), DNA was precipitated overnight and cleaned with 70% ethanol ...
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bioRxiv - Neuroscience 2020Quote: ... PCR assays were performed based on previous published method (Gonatopoulos-Pournatzis et al., 2018) OneStep RT-PCR kit (QIAGEN) was used according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... and 5 ng of cDNA was analyzed with isoform-specific dPCR assays and QIAcuity Probe PCR Kit according to manufacturer’s instructions (QIAGEN) in either 26k 24-well or 8.5k 96-well Nanoplates (QIAGEN) ...
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bioRxiv - Biochemistry 2020Quote: ... The relative TCF/LEF reporter activity was measured by the Cignal™ TCF/LEF Reporter Assay Kit (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Specific primer pairs (miScript Primer Assay or miRCURY LNA miRNA PCR Assay, Qiagen) for miR-142-5p and RNU48 were used ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked for 1 hour at room temperature either with a commercial blocking buffer (QIAGEN, anti RGS HIS6 HRP conjugate kit) or with 5% milk in 0.05% tween TBS buffer ...
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bioRxiv - Systems Biology 2020Quote: ... RNA and protein fractions using silica-membrane spin columns from the AllPrep DNA/RNA/Protein kit (cat# 80004, Qiagen, Chatsworth, CA, USA). PBMCs were processed in a randomized order ...
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bioRxiv - Microbiology 2020Quote: 250ml of stationary phase culture for each strain was filtered onto 0.2 μm Polyethersulfone (PES) membrane filters and DNA was extracted using the DNeasy PowerSoil kit (Qiagen, Germantown, MD, USA). Sequencing was done on an Illumina Hiseq ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Total RNA was purified on a silica membrane with the RNA clean-up protocol from the RNAeasy kit (Qiagen, Cat. No. 74104).