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Citations for Qiagen :
451 - 500 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... RNeasy 96 Kits (Qiagen) were used ...
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bioRxiv - Cancer Biology 2024Quote: FlexiGene DNA Kit (Qiagen) was used for isolation of DNA according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Transcription Kit (205314, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... Genomic DNA was isolated from EDTA anticoagulated blood according to the membrane-based QIAamp DNA extraction protocol (Qiagen, Hilden, Germany). The common genetic variants involved known to modulate the risk of fatty liver ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µL from the supernatant and cell lysates were applied on a nylon membrane and two different antibodies (an HRP-conjugated anti-His tag from Qiagen and an HRP-conjugated anti-strep tag from Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: Tissue cultures that were cultivated on one filter membrane (3 cultures) were transferred as one sample into RLT buffer (QIAGEN) and RNA was isolated according to the manufacturer’s instructions (RNeasy Plus Micro Kit ...
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bioRxiv - Genomics 2022Quote: ... We discarded the flowthrough of the PVDF membrane and transferred the membrane immediately after filtration to a 0.7 mm Garnet particle containing tube (13123-50, MoBio – now QIAGEN PowerBead) using sterile tweezers ...
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bioRxiv - Physiology 2024Quote: ... Sweden) or on Flinders Technology Associates (FTA) membranes (QIAcard FTA Elute Indicating Micro, Qiagen, specially tailored for PExA, PExA AB), a chemically coated cellulose membrane type that lyse cells on contact ...
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bioRxiv - Biophysics 2024Quote: ... the DNA fragments in the samples digested by MNase were purified in sterilized water using the silica membrane spin column (Qiagen). We repaired the purified DNA fragments using the NEBNext® Companion Module for Oxford Nanopore Technologies® Ligation Sequencing (New England Biolabs ...
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bioRxiv - Neuroscience 2024Quote: Visium spatial gene expression slides and reagents kits were used (10X Genomics; #1000187, Dual Index Kit TT Set A; #1000215) and RNeasy Kit (Qiagen; #74134) according to manufacturer instructions (10X Genomics) ...
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bioRxiv - Genomics 2024Quote: ... DNA was extracted from the samples using the QIAamp® DNA Micro kit or the DNeasy Blood & Tissue kit (spin column or plate extraction kits, Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Each reaction of the HRM assay included 6.25µl of Type-IT 2 x HRM buffer (QIAGEN, Germany), primers specific for the uidA E.coli housekeeping gene and molecular grade water was added to make a final volume of 12.5µl ...
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bioRxiv - Genomics 2020Quote: ... The primer used in the 3’ RACE assay was designed using CLC Genomic Workbench (ver. 10.1.1, Qiagen) near the highest CAGE-Seq signal in the determined TSS region ...
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bioRxiv - Microbiology 2020Quote: ... Viral genomic RNA (gRNA) was detected with a one-step real-time RT-PCR assay (Quantifast, Qiagen) using primers and probes generated to target either the SARS-CoV-2 E (28 ...
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bioRxiv - Microbiology 2020Quote: ... All TaqMan RT-qPCR assays were performed on the thermocycler Rotor Gene Q MDx (Qiagen, Courtaboeuf, France) and Lightcycler LC480 (Roche ...
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bioRxiv - Microbiology 2020Quote: ... ARTD14 and ARTD15 were analyzed by quantitative real-time PCR (qRT-PCR) using QuantiTect Primer Assays (QIAGEN). In all settings the mRNA expression of the gene of interest was normalized to GUS (forward 5’-CTCATTTGGAATTTTGCCGATT-3’ and reverse 5’-CCGAGTGAAGATCCCCTTTTTA-3’ ...
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bioRxiv - Immunology 2021Quote: ... Resulting cDNA served as template for the amplification of transcripts from Ubc (QT00245189, QuantiTect Primer Assay, Qiagen), Hprt (mm00446968_m1 ...
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bioRxiv - Neuroscience 2023Quote: ... The assay for MBP was tested for its sensitivity using the EpiTect PCR Control DNA Set (Qiagen). Product amplification was performed using the following reaction mixture ...
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bioRxiv - Developmental Biology 2022Quote: ... Gene expression of different fos family genes in zebrafish was analyzed using Quantitect™ Primer Assays (Qiagen) for each individual gene – fosaa ...
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bioRxiv - Microbiology 2023Quote: ... All TaqMan RT-qPCR assays were performed on the thermocycler Rotor Gene Q MDx (Qiagen, Courtaboeuf, France). Amplification was carried out according to the following thermocycling conditions ...
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bioRxiv - Neuroscience 2023Quote: Amplification and sequencing primers (Table S3) were designed by using the PyroMark Assay Design Software (v2.0, Qiagen) for the region of interest ...
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bioRxiv - Physiology 2023Quote: ... The cDNA used in the qPCR assays was first diluted with nuclease-free water (Qiagen, Hilden, Germany). Each qPCR mixture contained SsoAdvance Universal SYBR® Green Supermix ...
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bioRxiv - Microbiology 2023Quote: ... 100 nanograms of cDNA was used for qPCR analysis of the identified genes (Quantitect Primer Assay, Qiagen) using the iQ SYBR green supermix (BioRad Cat ...
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bioRxiv - Microbiology 2024Quote: ... All samples were run in multiplex (FAM, JOE, ROX) TaqMan assays on a Rotor Gene Q (Qiagen) using Takyon 2X MasterMix (Eurogentec ...
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bioRxiv - Genomics 2024Quote: ... All the primers (forward, reverse and sequencing primers) were designed with the PyroMark Assay Design software (Version 2.0.1.15, Qiagen) using the assay type "Methylation Analysis" (CpG ...
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bioRxiv - Microbiology 2024Quote: ... All TaqMan RT-qPCR assays were performed on the thermocycler Rotor Gene Q MDx (Qiagen, Courtaboeuf, France). Amplification was performed under the following thermocycling conditions ...
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bioRxiv - Biophysics 2024Quote: ... and bioinformatically validated specific primers (Gapdh, QT00199388; Rpl13a, QT00267197; Ifnb1, QT00249662; Trex1, QT00288967; QuantiTect Primer Assay, Qiagen). The final volume was 20 µL and the cycling conditions were a holding stage of 95°C for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... The specific bands were purified using gel purification kit (Qiagen gel purification kit). All these DNA amplified products were γ-32P radiolabelled by using T4 polynucleotide kinase enzyme using [γ-32P] ATP as the substrate with the reaction condition of 37°C for 30 min followed by 65°C for 25min enzyme inactivation period ...
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bioRxiv - Bioengineering 2020Quote: ... DNA was extracted using a commercially available kit (Qiagen DNeasy Blood & Tissue Kit). Afterwards ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... RNA was extracted using a commercial kit (RNeasy Micro kit, Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... DNA was extracted using a commercial kit (Qiagen Buccal Cell and DNAeasy Kit), as per the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and using the RNeasy Mini kit and the RNAase free DNAase kit (Qiagen). After RNA extraction ...
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bioRxiv - Microbiology 2020Quote: We used the DNeasy PowerWater Kit and the DNeasy PowerSoil Kit (Qiagen, Germany) according to the manufacturer’s instructions to extract DNA from the water and soil samples ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was isolated using RNeasy Micro Kit or RNeasy Mini Kit (Qiagen). After initial quality control using Agilent’s Bioanalyzer sequencing libraries were prepared from 500ng of total RNA per sample following Roche’s “KAPA stranded mRNA Seq” library preparation protocol for single indexed Illumina libraries ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was isolated with RNeasy Micro Kit or RNeasy Mini Kit (Qiagen). cDNA was synthesized using 1μg of mRNA with SuperScript™ III Reverse Transcriptase (Invitrogen 18080044) ...
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bioRxiv - Neuroscience 2021Quote: ... Bacterial DNA was extracted using a specific kit (QIAamp Powerfecal DNA kit, Qiagen), and its concentration was quantified by Nanodrop 2000 C Spectrophotometer (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... Resulting RNA was purified using a spin-column kit (RNeasy mini kit, QIAGEN) and 120 pmol of sgRNA were complexed with 100 pmol of recombinant SpCas9 protein at RT for 20 minutes ...
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bioRxiv - Microbiology 2023Quote: RNAs were isolated using the RNeasy mini kit mirVana RNA isolation kit (Qiagen). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... RNA was isolated via commercially available kit (Qiagen, RNeasy Mini Kit Cat# 74104) by lysing the cells in the vessel as per the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: Total genomic DNA was extracted using a commercial kit (DNeasy Tissue Kit, QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid DNAs were purified using QIAfilter Plasmid kits (Midi pre kit, Qiagen #12243) following manufacturer protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: High molecular DNA was extracted using the kit MagAttract HMW DNA kit (Qiagen) following manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2023Quote: ... and RNA was extracted using a commercially available kit (QIAGEN RNeasy mini kit). RNA was stored at −80°C until submission to the University of Minnesota Genomics Center (UMGC).
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bioRxiv - Immunology 2022Quote: Total RNA was isolated using RNeasy Mini kit or RNeasy Micro kit (QIAGEN). cDNA was synthesized with Oligo(dT ...
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bioRxiv - Physiology 2024Quote: ... Total RNA was isolated with an RNA purification kit (Qiagen miRNeasy mini kit) and DNase treatment to remove DNA contamination.
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bioRxiv - Biophysics 2024Quote: RNA extraction was performed using a commercially available kit (RNease Micro kit, Qiagen). RNA concentration and quality was monitored by spectrophotometry using the Nanodrop 2000 (Thermo Scientific).
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bioRxiv - Molecular Biology 2024Quote: miRNAs were extracted by miRNeasy Mini Kit and RNeasy MinElute Cleanup Kit (QIAGEN) and reverse transcribed with MystiCq® microRNA cDNA Synthesis Mix (Sigma-Aldrich) ...
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bioRxiv - Genetics 2024Quote: ... we used 16 different kits according to manufacturer’s instructions: EpiTect Bisulfite kit (Qiagen), EpiTect Fast bisulfite kit (Qiagen) ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant containing solubilized protein was filtered using a 0.22 μM nylon membrane filter and incubated with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) resin (Qiagen, Germantown, MD) at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The solubilized membrane fraction was loaded on a column containing 0.5 mL Strep-tactin Superflow plus resin (50% suspension; Qiagen, Hilden, Germany) equilibrated with 4 mL of the Wash buffer ...