Labshake search
Citations for Qiagen :
901 - 950 of 1933 citations for Mouse DPH7 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... All stable cell line plasmid transfections were conducted with Effectene (Qiagen, #301425) as per manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmids were routinely isolated using the Qiaprep Spin Miniprep kit (Qiagen, USA), and all primers were purchased from Integrated DNA Technologies (IDT ...
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bioRxiv - Genomics 2023Quote: ... grown overnight and purified using a Maxi plasmid purification kit (#12162; Qiagen).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the plasmid DNA were isolated using QIAprep Spin Miniprep kit (Qiagen) and Sanger sequenced (Laragen ...
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bioRxiv - Microbiology 2023Quote: ... The plasmids were extracted using QIAprep Spin Miniprep Kit (QIAGEN, Cat # 27104) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... All plasmids were verified by Sanger sequencing and purified with kit (QIAGEN) according to the manufacture’ protocol.
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bioRxiv - Biochemistry 2023Quote: ... plasmids were transiently co-transfected into HEK293T cells using PolyFect (Qiagen 301105) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The reporter plasmid was isolated using DNeasy Blood and Tissue Kit (QIAGEN), and the isolated plasmids were linearized with BglII and the copy number of encapsidated reporter plasmid was determined by qPCR using appropriate primers for the reporter gene in comparison to a standard curve.
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bioRxiv - Genomics 2022Quote: ... One-fifth of the cells was used for plasmid DNA extraction (Qiagen). The remaining cells were used for RNA extraction using the innuPREP RNA Mini Kit 2.0 (Analytik Jena) ...
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bioRxiv - Biochemistry 2022Quote: ... QIAGEN Plasmid Maxi Kits and QIAprep Spin Miniprep Kit were from QIAGEN. Zymo PUREII Plasmid Midiprep kit (D4200 ...
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bioRxiv - Cell Biology 2023Quote: ... coli bacteria (XL1Blue strain or DH5α strain) using plasmid extraction kits (QIAGEN; Venlo ...
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bioRxiv - Cancer Biology 2022Quote: ... and single colonies were isolated and grown for plasmid preparation (Qiagen #28115). The entire coding region of the HA-RAS cDNA were verified by Sanger Sequencing.
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bioRxiv - Genetics 2023Quote: ... Plasmid DNA was purified using the QIAprep Spin Miniprep Kit (Qiagen; 27106), digested with BsrGI ...
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bioRxiv - Genetics 2022Quote: ... The plasmid DNA was extracted using the QIAprep spin miniprep kit (Qiagen) and newly inserted sequences verified by Sanger sequencing.
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmid extraction and DNA purification were performed using kits purchased from Qiagen GmbH (Hilden ...
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bioRxiv - Cell Biology 2023Quote: ... pcDNA3-OCT4EGFP DNA was purified using a plasmid mini kit (QIAGEN, 12123). Correct orientation of the insert was confirmed by sanger sequencing.
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bioRxiv - Cell Biology 2023Quote: ... Purified plasmid DNA was isolated via a commercial spin miniprep kit (Qiagen), and cloning was confirmed via Sanger sequencing.
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bioRxiv - Synthetic Biology 2023Quote: ... and harvested the plasmid DNA using the Qiaprep Spin miniprep kit (Qiagen). We then transformed the GB1 library into yeast display Saccharomyces cerevisiae strain EBY100 made competent using the Zymo Research Frozen EZ Yeast Transformation II kit ...
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bioRxiv - Cancer Biology 2023Quote: ... We added 0.7 mL ETR buffer (From QIAGEN Plasmid Plus Midi Kit) to wash the column ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were routinely isolated using the Qiaprep Spin Miniprep kit (Qiagen, USA), and all primers were purchased from Integrated DNA Technologies (IDT ...
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bioRxiv - Immunology 2023Quote: ... The plasmid DNA was extracted using Qiagen HiSpeed Maxi kit (Qiagen, #122663) with one column per 2-gram bacterial pellet ...
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bioRxiv - Microbiology 2023Quote: ... Part plasmids were extracted using a QIAprep Spin Miniprep Kit (Qiagen, Germany) and sequence verified by whole plasmid sequencing (Plasmidsaurus ...
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bioRxiv - Bioengineering 2023Quote: ... Sequence-verified clones were purified using a Plasmid Plus Midi Kit (Qiagen), then purified again using a Nucleospin Gel and PCR Clean-Up Mini kit (Machery-Nagel) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and harvested the plasmid DNA using the Qiaprep Spin miniprep kit (Qiagen). We cut out the GB1 gene insert using XhoI and PstI restriction enzymes ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were prepared using a QIAprep Spin Miniprep Kit (QIAGEN cat. #27106) followed by ethanol precipitation for further purification ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were purified with the QIAprep Spin Miniprep Kit (QIAGEN, Hilden, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... Single colonies were then Midi-prepped (QIAGEN Plasmid Plus Midi Kit, 12943) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... Cultures were harvested and DNA purified using a Plasmid Giga Kit (Qiagen). Purified DNA was resuspended in buffer TE and adjusted to a concentration of ∼1 g/L ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were purified using the QIAprep Spin Miniprep Kit (QIAGEN, Hilden, Germany). First ...
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bioRxiv - Bioengineering 2023Quote: ... Recombinant plasmids were prepared by using the QIAprep Spin Miniprep Kit (QIAGEN) and confirmed by sequencing analysis ...
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bioRxiv - Developmental Biology 2024Quote: ... plasmids were purified from positively screened colonies with QIAprep Miniprep Kit (QIAGEN) and sequenced at the Research Institute for Molecular Genetics of Kochi University ...
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bioRxiv - Molecular Biology 2024Quote: ... plasmid purification was done using the QIAprep Spin Miniprep Kit (QIAGEN GmbH).
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bioRxiv - Molecular Biology 2021Quote: ... the membrane was incubated with mouse primary antibody α-His (1:5000, Monoclonal mouse Tetra-His antibody, Qiagen, #34670) in 2.5% BSA in PBS-T overnight at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... coli BL21 from pET16b-based plasmids and affinity purification via Ni-NTA (Qiagen), followed by specificity tests (Supplemental Figure 14).
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bioRxiv - Biophysics 2021Quote: ... The pJet CLN3 plasmid was linearized using PCR and gel purified (QIAGEN 28706) to create the template for in vitro transcription ...
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bioRxiv - Cell Biology 2020Quote: ... and then plasmid was purified using a QIAprep Spin Miniprep Kit (Qiagen, 27106) according to manufacture protocol.
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bioRxiv - Molecular Biology 2021Quote: ... and plasmids were subsequently isolated using Qiagen mini-prep kits (Qiagen; Cat #: 27104).
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bioRxiv - Molecular Biology 2021Quote: ... Small-scale isolation of plasmid DNA was performed with Mini-Prep kits (Qiagen); large-scale DNA plasmid purification was done with GenElute HP Endotoxin-Free Plasmid Maxiprep Kits (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... pGRG36-STM0233 and pGRG36-chiA were isolated using the Plasmid Midi kit (Qiagen), and Sanger sequencing was performed to confirm that the cloned chitinase genes had the correct sequence ...
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bioRxiv - Immunology 2021Quote: ... The plasmid DNA was amplified and purified using a GIGA prep (Qiagen, USA).
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bioRxiv - Developmental Biology 2020Quote: ... Plasmids containing the PCR product were prepped with the Spin Miniprep Kit (Qiagen) and sent to the RTSF Genomics Core at Michigan State University for Sanger sequencing.
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bioRxiv - Developmental Biology 2020Quote: ... Plasmids were extracted from bacteria culture with QIAprep Spin Miniprep Kit (Qiagen, #27104). One clone with a successful integration was used to perform in vitro mRNA synthesis with mMESSAGE mMACHINE™ SP6 Transcription Kit (ThermoFischer Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transiently transfected with mammalian protein expressing plasmids using Effectene (Qiagen, Germany) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... and purified using the QIAGEN Plasmid Maxi Kit (Cat# 12162, QIAGEN, Hilden, Germany), then transfected into cells using Lipofectamine 3000 (Invitrogen/Thermo Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... Lentiviral plasmids and packaging vectors were prepared using endo-free maxiprep kits (Qiagen). The pGK::GFP-LucOS::EFS::FlpO lentiviral plasmid was cloned using Gibson assembly (Akama-Garren et al. ...
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bioRxiv - Cancer Biology 2019Quote: ... Lentiviral plasmids and packaging vectors were prepared using endo-free maxiprep kits (Qiagen). Lentiviruses were produced by co-transfection of 293FS* cells with Lenti-LucOS or FlpO-GFP-OS ...
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bioRxiv - Molecular Biology 2020Quote: ... Small-scale isolation of plasmid DNA was done using QIAprep miniprep kit (Qiagen) from 2 mL of overnight culture ...
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bioRxiv - Bioengineering 2019Quote: ... The plasmid isolation and gel extraction kits were obtained from Qiagen (Hilden, Germany). The pET Express & Purify kits (In-Fusion Ready ...
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bioRxiv - Genetics 2019Quote: ... plasmid DNA was isolated using the QIAprep Spin Miniprep Kit (Qiagen, CA, USA), and individual clones were sequenced using BigDye® cycle sequencing chemistry.
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bioRxiv - Bioengineering 2019Quote: ... Plasmids for hydrodynamic tail-vein injection were prepared using the EndoFreeMaxi kit (Qiagen). For hydrodynamic liver injection ...