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Citations for Qiagen :
751 - 800 of 1003 citations for Zika Virus Lysate PRVABC59 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: RNA was isolated from frozen RNA later® soaked tissue from the embryo from either C57BL/6NCrl or Ddi2PD strains using RNeasy plus Micro and Mini Kits (QIAGEN). Samples with RIN values above 7 in the Bioanalyzer RNA 6000 Nano assay (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant protein fragments were produced in Escherichia coli (strain M15) as fusion products with an N-terminal tag of six histidines using plasmid pQE30 (Qiagen) and purified from total bacterial lysates by nickel affinity chromatography ...
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bioRxiv - Microbiology 2022Quote: ... Transformation of gene knockouts from strains 168 or PY79 into strain 3610 was done with the following protocol: DNA was extracted from strain 168 or PY79 with the DNeasy Blood & Tissue kit (Qiagen) as described in the manufacturer’s protocol and the final DNA yield was quantified by Qubit (Life Technologies) ...
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bioRxiv - Microbiology 2019Quote: ... all clinical isolates and the UCBPP-PA14 reference strain were cultivated at 37 °C in LB broth and harvested in RNAprotect (Qiagen) at OD600= 2 ...
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bioRxiv - Epidemiology 2019Quote: The genomic DNA of the 11 outbreak strains was extracted using the DNeasy blood and tissue kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: DNA extraction from cultured tprAko-SS14 or wild-type strains propagated in 6-well plates following the transformation procedure was performed using the QIAamp mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Most DNA samples for ONT sequencing were extracted from 100 female flies from each strain using the Blood and Cell Culture DNA Mini Kit (Qiagen) following manufacturer’s instructions with small modifications (Table S2 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we designed strain-specific primers (see Supplementary Table 2) and generated overlapping amplicons using the OneStep RT-PCR Kit (Qiagen) and 5 µL of cDNA ...
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bioRxiv - Biochemistry 2021Quote: Genomic DNA was isolated from 2 clones of each strain corresponding to 50 OD600 units with the Genomic DNA Buffer Set and Genomic-tip 100/G (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: The gDNA of all mutants strains constructed in this study was extracted using DNeasy® Blood and Tissue kit (Qiagen) according to the manufacturer recommendations ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA from 110 mutants plus the ancestral strain was extracted with the Genomic-tip 20/G Kit (Cat. No. 10223, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... coli HST04 dam-/dcm- strains in which target MTases were transformed were extracted using the DNeasy UltraClean Microbial Kit (QIAGEN) according to the supplier’s protocol after induction of gene expression ...
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bioRxiv - Genetics 2023Quote: Total RNA was extracted from MU636 and set1 mutant strains growing in plates using the RNeasy Plant Mini Kit (Qiagen) and treated with DNase (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... We extracted gDNA from the MSK named strains collected in Botswana and North Carolina using the Powersoil Pro extraction kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... coli strain M15-[pREP4] (for transformation of recombinant plasmids) and RNAse (for protein purification) were purchased from Qiagen (Valencia, CA). All other chemicals were obtained from Sigma-Aldrich (St ...
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bioRxiv - Molecular Biology 2024Quote: ... coli XL1-blue strain was transformed and plasmids were isolated from bacterial cultures using the Plasmid Midi Kit (Qiagen, Germany). Sequencing of the three NS1 plasmids was performed by Eurofins (Germany).
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bioRxiv - Microbiology 2024Quote: ... the raw FASTQ sequencing data for RNAseq (see above) was combined for each strain across each condition and were imported into CLC Genomics Server v23 (QIAGEN Digital Insights ...
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bioRxiv - Molecular Biology 2024Quote: RNA isolation from three independent biological replicates of the strains GJV1 and GJV1Δpxr was done using the RNeasy Mini Kit (QIAGEN, # 74004) according to the manufacturer’s protocol using cells at mid-log phase ...
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bioRxiv - Molecular Biology 2024Quote: ... in chromosomes 6 and 15 using a Multiplex PCR blind strategy and genomic DNA isolated from HeLa-JVM and HeLa-HA strains (using a DNeasy Blood & Tissue Kit (Qiagen) and following the manufacturer′s instructions) ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were then processed for RNA and DNA extraction using AllPrep DNA/RNA Mini kit (Qiagen), with Complete Protease Inhibitor (Roche ...
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bioRxiv - Molecular Biology 2020Quote: RNA extractions from frozen lysates were performed on the same day using RNeasy Mini columns (Qiagen). Recovered total RNA was quantified by Qubit and quality was assessed using an Agilent Bioanalyzer ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysate was cleared by centrifugation and the soluble fraction was applied to Ni-NTA resin (Qiagen) and incubated for 1.5 h ...
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bioRxiv - Microbiology 2019Quote: ... RNA was then isolated from cleared lysate using the manufacture’s protocol (#74106 RNeasy Mini Kit Qiagen).
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bioRxiv - Genetics 2020Quote: DNA/RNA extraction was performed from cell lysates using the AllPrep DNA/RNA Micro Kit (Qiagen). For initial optimisation experiments using an HPRT gRNA-containing RNP (or non-targeting control) ...
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bioRxiv - Genetics 2020Quote: ... Total DNA was isolated from the cell lysates using the QIAquick® PCR Purification Kit (QIAGEN). The following primers used were designed to the lucia DNA sequence for amplification of the reporter gene (JE041 ...
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bioRxiv - Microbiology 2021Quote: ... Toxins were then purified from bacterial cell lysates through affinity chromatography on a Nickel-resin (Qiagen), ion exchange ...
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bioRxiv - Biochemistry 2020Quote: ... Clarified lysate from bacterial expression was passed over a column with Streptactin XT Superflow resin (Qiagen). After incubation ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared by centrifugation and the supernatant was applied to Ni NTA-agarose (Qiagen), which was washed with the same buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble lysate fraction was bound in batch to nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen). The resin was washed extensively with 20 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble lysate fraction was bound in batch to nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen). The resin was washed extensively with 20 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... The clarified lysate was bound to a 2 mL slurry of Strep-Tactin Superflow plus (Qiagen) overnight at 4°C using the batch method ...
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bioRxiv - Microbiology 2021Quote: ... nucleic acids were isolated from cell lysates or lung homogenates using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: The clarified lysate was then loaded onto an open column containing pre-equilibrated Ni-NTA (Qiagen). The column was washed with 20 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: ... The lysate was then transferred to the RNeasy spin column provided in the RNeasy kit (Qiagen). The manufacturers protocol was then followed for the rest of the isolation process.
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bioRxiv - Cell Biology 2020Quote: ... The lysate was centrifuged and the supernatant was load onto a Ni-NTA affinity column (Qiagen). Proteins were eluted with elution buffer (20 mM Tris (pH 8.0) ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified lysate was first purified on a Ni–nitrilotriacetic acid (NTA) column (QIAGEN, Valencia, CA). The eluate was further purified on glutathione–Sepharose 4B resin (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleared lysate was purified over Ni-NTA affinity resin according to the manufacturer’s recommendation (Qiagen). Eluted proteins were pooled and dialyzed overnight at 4°C into 50 mM Na2HPO4 pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... The bacterial lysates were mixed for 1 h with 5 ml of Ni-NTA resin (Qiagen) that had been equilibrated with buffer A ...
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bioRxiv - Cell Biology 2020Quote: ... cell lysates were spun on the QIashredder column and RNA extracted using the RNeasy kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... and the supernatant of the bacterial lysate was loaded to a disposable column (Qiagen, Hilden, German) pre-packed with glutathione agarose (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified lysate was applied to two tandemly-connected 5 ml Ni-NTA Superflow cartridges (Qiagen); the cartridges were washed with buffer containing 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... mouse organ lysates and human primary cell supernatant samples were prepared in RNeasy Mini Kit (Qiagen) lysis buffer RLT (400μl) ...
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bioRxiv - Cell Biology 2021Quote: ... Lysate was cleared by centrifugation and then applied to a column of Ni-NTA resin (Qiagen). The column was washed with 10 column volumes (CVs ...
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bioRxiv - Immunology 2020Quote: ... Total RNA was isolated from Trizol/cell lysate mixture by using RNAeasy Mini Kit (Qiagen #74104). Total RNA was sequenced on Illumina NextSeq using Illumina TruSeq Stranded Total RNA library Prep and paired-end sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... Further these lysates were incubated overnight at 4°C with prewashed Ni-NTA beads (Qiagen, 30210).Sequential washes with wash buffer 1-4 ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted and purified from the lysate using the DNeasy Blood and Tissue Kit (Qiagen). The DNA from the sediments was extracted from 2 g using a sodium phosphate buffer and concentrated into 50 KDa amicon filters ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were incubated at 37°C for 15 minutes following addition of 2µg RNase A (Qiagen). 1pmol ssDNA substrate 5’ DY782 ATTATTATTATTATTATTATTTCATTTATTTATTTATTTA-3’ (Eurofins ...
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bioRxiv - Molecular Biology 2024Quote: ... The fusion protein in the lysate was affinity-purified by Ni-NTA agarose beads (Qiagen, 30210) capturing HIS tag or by glutathione sepharose 4B beads (Amersham ...
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bioRxiv - Microbiology 2023Quote: ... total RNA of infectious cell lysate was extracted using an RNeasy Mini Kit (QIAGEN, Hilden, Germany). RT–PCR was performed using a Superscript III One-Step RT-PCR kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were then incubated for 1 h with nickel-nitriloacetic acid (Ni-NTA) beads (Qiagen) at the indicated temperatures (4°C or room temperature) ...