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Citations for Qiagen :
501 - 550 of 1003 citations for Zika Virus Lysate PRVABC59 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and RNA from cell lysates was extracted using a RNeasy Mini Kit (Qiagen) as described previously (20 ...
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bioRxiv - Immunology 2020Quote: ... The clear solution containing lysate was passed through RNeasy mini column (Qiagen, Germany), which leads to the binding of RNA to the column.
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bioRxiv - Biochemistry 2021Quote: ... The cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed in two steps with lysis buffer supplemented with 25 and 50 mM imidazole ...
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bioRxiv - Immunology 2021Quote: Total RNA was isolated from tissue lysates using the RNeasy Mini Kit (Qiagen), including on-column DNase digestion ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from the tumor lysates with a RNeasy Mini kit (Qiagen) and was reverse transcribed with a First Strand cDNA synthesis kit (Fermentas) ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysate was loaded on a 10 ml Ni-NTA Superflow column (QIAGEN), washed with 7 column volumes of 20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... Clarified lysate was loaded to a column prepared with Ni-NTA resin (Qiagen). Resin was washed with lysis buffer containing 60 mm imidazole and then protein was eluted with 400 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was passed through 2 mL of Ni-NTA beads (Qiagen), equilibrated with 50 mM Tris-HCl ...
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bioRxiv - Evolutionary Biology 2022Quote: ... This lysate was used for RNA extraction with an RNeasy Mini Kit (Qiagen) according to the manufacturer’s standard protocol for bacterial RNA purification.
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bioRxiv - Microbiology 2022Quote: RNA was extracted from infected cell lysates using RNeasy® Mini Kit (QIAGEN) with an on-column DNase treatment (QIAGEN ...
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bioRxiv - Microbiology 2022Quote: ... Clarified lysate was passed over a Ni2+ affinity column (Ni-NTA Superflow, Qiagen) and eluted in a buffer with 250 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... The resulting lysate was used as input for the RNeasy Mini kit (Qiagen) including an on-column DNase treatment (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... RNA was extracted from whole-cell lysates with RNeasy Plus Mini kit (Qiagen) and was reverse-transcribed with cDNA synthesis kit (Thermo Fisher) ...
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bioRxiv - Pathology 2024Quote: ... RNA was extracted from the lysate using the Qiagen RNeasy mini kit (Qiagen) according to the manufacturer’s instruction.
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was applied to gravity flow Ni-NTA agarose resin (QIAGEN) previously equilibrated with wash buffer WB4_1 (50 mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was passed through 2 mL of Ni-NTA beads (Qiagen), equilibrated with buffer [50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was passed through 2 mL of Ni-NTA beads (Qiagen), equilibrated with buffer [50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was loaded on to 12ml of NiNTA agarose beads (Qiagen, 30250) and batchbound for 1.5 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... and DNA purified from lysate using a DNeasy Blood & Tissue Kit (QIAGEN, 69504) with slight modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was passed through 2 mL of Ni-NTA beads (Qiagen), equilibrated with buffer [50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was passed through 2 mL of Ni-NTA beads (Qiagen), equilibrated with buffer [50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein was isolated from the lysate using Ni-NTA Agarose resin (Qiagen) and was purified to homogeneity and >95% purity by size exclusion chromatography using a HiLoad Superdex 75 pg 16/600 column (Cytiva ...
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bioRxiv - Molecular Biology 2022Quote: ... The cleared lysate was incubated with 0.25 ml of Ni-NTA beads (Qiagen) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... according to manufacturer’s instructions and lysates were homogenized through QiaShredder columns (Qiagen, Switzerland). The RNeasy Mini Kit (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... The cleared lysate was incubated with pre-equilibrated Ni-NTA Agarose beads (Qiagen) for 4 h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: TRAP and total lysate RNA were isolated via the RNeasy Plus Kit (Qiagen). The samples were first lysed and homogenized in Buffer RLT Plus ...
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bioRxiv - Immunology 2023Quote: Total RNA was isolated from cell lysates using the RNeasy Micro Kit (QIAGEN), including on-column deoxyribonuclease digestion ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was isolated from the lysate using miRNeasy Mini kit (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was prepared from cell lysates using the RNeasy Mini Kit (Qiagen) and RNase-Free DNase Set (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was passed through 2 mL of Ni-NTA beads (Qiagen), equilibrated with buffer [50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... cell lysates were either mock-treated or treated with RNase A (Qiagen 19101) and then incubated at 4°C for 2 hours with biotinylated oligos ...
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bioRxiv - Biochemistry 2023Quote: ... Filtered cell lysate was applied to 10 mL of Ni-NTA resin (Qiagen) which had been previously equilibrated with 10 column volumes of H2O ...
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bioRxiv - Bioengineering 2023Quote: ... RNA extraction was performed from each cell lysate employing a miniprep kit (Qiagen). The RNA quality was analyzed using the 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... LdcAEc was purified from the cleared lysates via Ni-NTA agarose columns (Qiagen) and eluted with a discontinuous imidazole gradient using an ÄktaGo system ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was isolated and purified from the tissue lysates using miRNeasy columns (Qiagen) according to the an fact rer’s instr ctions ...
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bioRxiv - Developmental Biology 2024Quote: ... The lysates were column purified using QiaAMP DNA mini-Kit (Qiagen, Cat # 56304) and DNA quality of the eluate was determined using Nanodrop 2000 Spectrophotometer (#E112352 ...
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bioRxiv - Genomics 2024Quote: ... The lysate was collected and purified with MinElute Reaction Clean-up column (Qiagen), and eluted in 25 μL of EB buffer ...
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bioRxiv - Cancer Biology 2024Quote: Protein lysates from snap-frozen tissue were prepared using the TissueLyser II (Qiagen) and RIPA lysis buffer (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... RNA from one aliquot per condition per virus isolate and negative control was immediately extracted with the QIAamp Viral RNA Mini Kit (Qiagen) and stored at −80°C until further processing ...
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bioRxiv - Microbiology 2021Quote: Total RNA of each isolate was extracted from frozen virus stock using QIAamp Viral RNA Mini Kit (Qiagen, Germantown, MD) following the manufacturers spin protocol ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from the working stock of each reporter virus and treated with DNase using the DNase Max kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: 200µL of lung clarified homogenate or infectious cell supernatant (virus stock) was inactivated with an equal volume of VXL lysis buffer (Qiagen) and viral RNA was extracted using an EZ1 Advanced XL robot with the EZ1 mini virus 2.0 kit (both from Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... RNA was extracted from 70 μL of supernatant using QIAamp 96 virus QIAcube HT kit on the QIAcube HT System (Qiagen). Each RNA sample was analyzed by one-step RT-qPCR with two SYBR Green assays ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was extracted from supernatants of swab material using the QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony instrument (Qiagen). RT-qPCR was then performed using the AgPath RT-PCR (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Total Nucleic Acid (TNA) was extracted from using automated nucleic acid extraction on the QIAsymphony and the DSP Virus/Pathogen Mini Kit (Qiagen). Autopsy tissues were collected from lung ...
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bioRxiv - Microbiology 2019Quote: For viral genome copy measurements RNA was extracted from 2 µl sucrose purified virus using the RNeasy mini kit (QIAgen). The RNA was then treated with TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from supernatants of hamster nasal wash samples using the QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony instrument (Qiagen). Real time RT-qPCR was then performed using the AgPath RT-PCR (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: Viral RNA extraction was performed on an EZ1 Advanced XL device using the EZ1 virus mini kit V2.0 according to the manufacturer’s recommendations (Qiagen, Courtaboeuf, France). The qPCR was run on a Lightcycler® 480 thermocycler (Roche diagnostics ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from cell culture supernatants or hamster nasal washes using the QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony instrument (Qiagen). RT-qPCR was then performed using the AgPath RT-PCR (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: Total viral RNA was extracted from deactivated samples using automated nucleic acid extraction on the QIAsymphony and the DSP Virus/Pathogen Mini Kit (QIAGEN). One step reverse transcription to cDNA and real-time PCR (RT-PCR ...