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Citations for Qiagen :
701 - 750 of 1003 citations for Zika Virus Lysate PRVABC59 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... Lysate was spun down and supernatant was mixed with 2 ml Ni-NTA resin (Qiagen) per 50 ml sample and incubated for 1h at 4°C ...
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bioRxiv - Biophysics 2019Quote: ... All the proteins were obtained in cell lysates and subsequently purified using Ni-NTA (Qiagen) affinity-columns and superdex 200 increase 10/300 GL (GE Lifesciences ...
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bioRxiv - Microbiology 2021Quote: ... the clarified lysate was bound using the batch method to Ni-NTA agarose resin (Qiagen) at 4°C for 1 hour ...
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bioRxiv - Genomics 2019Quote: ... The cell lysate was sampled (100 μL) and mixed with 700 μL of QIAzol (QIAGEN) for total RNA extraction ...
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bioRxiv - Biochemistry 2020Quote: ... lysate was clarified by centrifugation and the supernatant was incubated on Ni-NTA resin (Qiagen). The resin was washed with wash buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... coli lysates under native conditions by immobilised metal affinity chromatography using Ni-NTA agarose (Qiagen) essentially as described 12 using 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... the soluble fraction of the lysate was loaded onto a Ni-NTA Superflow Cartridge (QIAGEN) pre-equilibrated in wash buffer (50 mM HEPES-Na ...
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bioRxiv - Biochemistry 2021Quote: ... Lysate was then batch bound to 5 mL of Ni-NTA Agarose (Qiagen, Cat# 30230) resin at 4°C for 1 hour in a beaker set on a stir plate ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleared lysates were mixed with 0.5 ml Ni-NTA beads (250 μl bed volume, Qiagen), pre-washed with two times 5 ml lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... The clarified cell lysate was loaded onto a 5 mL Ni-NTA superflow cartridge (Qiagen), washed with buffer A ...
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bioRxiv - Immunology 2021Quote: RNA extraction was performed on the lysate using the RNeasy Mini Kit (Qiagen, Hilden, Germany). Libraries were prepared as previously described.22 Briefly ...
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bioRxiv - Microbiology 2022Quote: ... The filtered lysate was then loaded onto a 5 ml Ni-NTA Superflow column (Qiagen), and the column was washed with buffer A containing 10 mM imidazole ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was immediately isolated from the lysate using the RNeasy kit (Qiagen, Germantown, MD), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... lysate samples were thawed at room temperature and then processed using QIAshredder (Qiagen, Cat# 79654) and Rneasy Mini Kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was extracted from the lysate using the DNeasy® Plant Mini Kit (QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... RNA was extracted from RLT Plus lysates using the RNeasy Plus Mini Kit (QIAGEN, #74136), including on-column DNase treatment using the RNase-Free DNase set (QIAGEN ...
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Phospho-KNL-1 recognition by a TPR domain targets the BUB-1–BUB-3 complex to C. elegans kinetochoresbioRxiv - Cell Biology 2024Quote: ... 6xHis tagged proteins were purified from the clarified lysates by using Ni-NTA beads (Qiagen), and incubated with end-over-end rotation overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was briefly incubated (approximately 5 min) with 3 ml Ni-NTA Agarose (QIAGEN) before flow-through was collected ...
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bioRxiv - Cell Biology 2023Quote: ... The cleared lysate was purified by affinity chromatography on a Ni-NTA Superflow Cartridge (Qiagen) using an ÄKTA Start (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... The cleared lysate was purified by affinity chromatography on a Ni-NTA Superflow Cartridge (Qiagen) using an ÄKTA Start (Cytiva ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The cleared lysate was purified by affinity chromatography on a Ni-NTA Superflow Cartridge (Qiagen) using an ÄKTA Start (Cytiva ...
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bioRxiv - Biochemistry 2022Quote: ... coli lysates under native conditions as described in the fifth edition of the QIAexpressionist (Qiagen). The lysates were loaded onto a 1 ml HisTrap HP column (GE healthcare ...
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bioRxiv - Microbiology 2023Quote: ... was further added to the lysate prior to using a bead beater (TissueLyser II, Qiagen) for homogenizing the mixture for 5 min at a speed of 30 beats/sec ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant lysates were purified on Ni-NTA agarose beads (for His6-tagged proteins, Qiagen) or Glutathione High Capacity Magnetic Agarode Beads (for GST-tagged proteins ...
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bioRxiv - Biochemistry 2023Quote: ... Lysate was then batch bound to 5 mL of Ni-NTA Agarose (Qiagen, Cat# 30230) resin at 4ºC for 2 hours in a beaker set on a stir plate ...
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bioRxiv - Genomics 2023Quote: ... RNA from the lysate was extracted and purified with an RNeasy Mini Kit (QIAGEN #74104) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The cleared lysate was loaded onto two 5 mL Ni-NTA Superflow cartridges (QIAGEN, 30761) equilibrated with lysis buffer and washed with 10 column volumes of washing buffer containing 20 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysate was passed over a gravity column of 8 mL of Ni-NTA resin (Qiagen), washed with 70 mL wash buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... The lysate was centrifuged and the soluble cell fraction was incubated with Ni2+ –NTA (Qiagen) beads for 3 hr ...
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bioRxiv - Microbiology 2023Quote: ... the lysate was processed using the described protocol of the RNeasy Mini kit (Qiagen, Germany). Finally ...
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bioRxiv - Cancer Biology 2023Quote: ... The clear lysate was transferred to a QIAilter Catridge (From QIAGEN Plasmid Plus Midi Kit) and incubated at room temperature for 10 mins ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was then incubated with 5 mL of Ni-NTA agarose beads (Qiagen), pre-equilibrated in lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... The filtered lysate was then passed over a 5 mL Ni-NTA Superflow column (Qiagen). Protein elution was carried out with a linear gradient of modified buffer B (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... The filtered lysate was then passed over a 5 mL Ni-NTA Superflow column (Qiagen). The column was washed extensively with buffer A and subsequently ...
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bioRxiv - Biochemistry 2024Quote: ... The filtered lysate was then passed over a 5 mL Ni-NTA Superflow column (Qiagen). Protein elution was carried out with a gradient of buffer B ...
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bioRxiv - Microbiology 2024Quote: ... Purification of total RNA from bacterial lysate using the RNeasy® Mini Kit (Qiagen, Germany) following protocol 7 in the handbook ...
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bioRxiv - Microbiology 2024Quote: ... The cell lysates obtained after centrifugation were subjected to a Ni2+-NTA affinity column (Qiagen). The immobilized proteins underwent a stepwise wash with lysis buffer containing 10 mM and 20 mM imidazole ...
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bioRxiv - Molecular Biology 2024Quote: pLicC-MBP-ERR1712142 plasmid was extracted from bacterial lysate with Maxiprep (Qiagen Cat No. 12662) after overnight incubation in BL21 strain BL21 (λDE3 ...
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bioRxiv - Microbiology 2024Quote: ... and the remaining lysates were incubated with 1 mL of Ni-NTA agarose (Qiagen, 1018244) on nutator (Daeiltech ...
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bioRxiv - Microbiology 2020Quote: ... Viral nucleic acids were then isolated using the Qiagen QIAamp MinElute Virus Spin Kit without the use of AW1 buffer or carrier RNA (Qiagen, Valencia, CA, USA). Random hexamers were used to prime cDNA synthesis (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed in 384 well plates on an Applied Biosystems 7900HT Fast Real-Time PCR system using the QuantiTect Virus Kit (Qiagen, Redwood City, CA) and SARS-CoV-CDC RUO primers and probes (Integrated DNA Technologies (IDT) ...
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bioRxiv - Genomics 2022Quote: ... Nucleic acids were extracted at CNM using either QIAamp MinElute Virus Spin (DNA) or QIAamp Viral RNA Mini kits (Qiagen, Germantown, MD, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2021Quote: Genomic DNA was extracted for the ancestral strain (AN) and a single clonal isolate from each evolved population (S1-S10) using QIAamp DNA Blood Midi Kit (Qiagen) following the manufacturer’s recommendations from 50 mL culture incubated at 17 °C for 5 days in 75 cm2 flasks ...
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bioRxiv - Genomics 2022Quote: ... The genomic DNA of the wild-type and the mutant strains was isolated using a QIAamp DNA Mini Kit (QIAGEN). Libraries were prepared using the Nextera XT DNA Library preparation kit (Illumina) ...
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bioRxiv - Molecular Biology 2020Quote: ... coli strain (Rosetta) and purified using Ni-NTA affinity chromatography according to the manufacturer’s instructions (Qiagen Cat No./ID: 30210). Purified protein was used as an immunogen to raise polyclonal antibody in rabbits at a local commercial facility (DPL ...
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bioRxiv - Microbiology 2019Quote: ... The complete 16S rRNA gene was amplified in specific strains after DNA extraction using the DNeasy Blood & Tissue kit (Qiagen), following the manufacturer’s recommendations ...
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bioRxiv - Genomics 2019Quote: Genomic DNA from each strain was isolated from overnight cultures using the DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... genomic DNA was prepared from 20 adult female flies from each strain using the DNeasy Blood and Tissue kit (Qiagen). PCR was performed with primers specific to the LcαE7 gene16 ...
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bioRxiv - Microbiology 2021Quote: ... 500 μL of mid-log phase (OD600nm = 0.45 - 0.7, strain-specific) cells were stabilized using RNA Protect Bacteria Reagent (QIAGEN, Hilden, Germany), and cell pellets stored at −80°C ...
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bioRxiv - Microbiology 2021Quote: ... and RNA was isolated from 3 biological replicates of each strain when the cultures had reached OD600 = 0.1 using the RNeasy mini kit (Qiagen #74106). RNA-Seq was performed at the Genomics Core Facility at Princeton University ...