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Citations for Qiagen :
701 - 750 of 5525 citations for Primary Human Brain Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA was prepared from cell pellets (1 million cells) using Allprep DNA/RNA mini kit (Qiagen). Whole genome libraries were sequenced 150x2 bp on a NovaSeq 6000 (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... RPE1 cells or HeLa FlpIn cells were transfected with siRNAs at 100 nM using the HiPerFect (Qiagen), according to manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... total DNA from 1×106 cells was extracted using the Blood & Cell Culture DNA Mini Kit (Qiagen). Extracted DNA was diluted to 10 ng/μl and subjected to qPCR analysis ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from CD11b+ cells and LK cells using RNeasy plus mini kit (Qiagen 74134) [source?] ...
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bioRxiv - Cell Biology 2024Quote: HAEC cells from the stretch experiments were harvested by cell scraping and resuspended in RLT buffer (Qiagen) supplemented with β-Mercaptoethanol ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmids were isolated from cell pellets using the EndoFree Plasmid Maxi Kit (0.45 g cells/column; Qiagen, 12362). Sequencing-based barcode quantification (see below Quantifying barcodes from plasmids and rabies virus genomes ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA from control cells and trRpn13 MM cells were extracted by QIAamp→ DNA Mini Kit (51304; Qiagen). DNA editing status was validated by performing TOPO blunt cloning of PCR amplicons encompassing the guide RNA target sites and Sanger sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... CD11b+ magnetic sorted microglia cells or embryonic neural progenitor cells were homogenized in QIAzol Lysis Reagent (#79306, QIAGEN) either immediately after isolation or after 10 days of in vitro culturing ...
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bioRxiv - Cell Biology 2021Quote: Cell pellets of 100,000 cells were prepared and RNA was extracted using the RNeasy Mini Kit (Qiagen, #74104). Stranded mRNA (polyA-selected ...
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bioRxiv - Genomics 2022Quote: Total RNA was isolated from cell pellets (<1×107 cells) using the RNeasy Mini kit (QIAGEN, cat. 74134). Cells were lysed with RLT buffer (with no ß-ME ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA from MCF-7 cells (1×106 cells/well) was isolated using RNeasy Mini kit (74106; Qiagen), and 500ng cDNA was synthesized with random hexamers by reverse transcription (SuperScript III ...
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bioRxiv - Neuroscience 2022Quote: ... the cell viability was assessed and cells were then resuspended in 350µl RLT Lysis buffer (Qiagen; Cat #: 74136) containing 2-Mercaptoethanol (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then washed 3 times with PBS and lifted with 500µL/well of Cell Protect Reagent (Qiagen) for 5 min ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was extracted using the Blood & Cell Culture DNA Mini (<5M cells) Kits (Qiagen, cat. no. 13323) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... cells were harvested and genomic DNA was isolated using the “Blood & Cell Culture DNA Mini Kit” from Qiagen and according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: RNA was prepared from cultured tumor cells or sorted cells from implanted tumors using RNeasy Mini Kit (QIAGEN). cDNA was generated using High-capacity cDNA Reverse Transcription Kit (Bio-Rad) ...
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bioRxiv - Clinical Trials 2019Quote: Genomic DNA from 1-10 Million CD4+ T cells was isolated using the Gentra Puregene Cell Kit (Qiagen). In some experiments ...
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bioRxiv - Cancer Biology 2019Quote: ... and Treg (DAPIneg, i.v. neg, Thy1.2+CD4+Foxp3-GFP-positive) cells were single-cell sorted into Buffer TCL (Qiagen) plus 1% B-mercaptoethanol in 96-well plates using a MoFlo Astrios cell sorter ...
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bioRxiv - Cell Biology 2020Quote: RNA was collected from siRNA-treated HeLa cells and extracted using the RNeasy PowerLyzer Tissue & Cells Kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: DAFhi and DAFlo GC B cells (CD19+ CD20+ CD38+ IgD−) were resuspended in RLT cell lysis buffer (Qiagen) after flow cytometric sorting ...
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bioRxiv - Cancer Biology 2022Quote: ... Each population of filtered cells as well as nonfiltered nontreated cells were lysed using a QIAshredder Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Each population of filtered cells as well as nonfiltered nontreated cells were lysed using a QIAshredder Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from cell pellets using the Blood & Cell Culture DNA Maxi Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... genomic DNA was isolated from HEK293T cells 3 days after transfections using the Gentra Puregene Cell Kit (Qiagen) and was diluted to 10 ng/μL ...
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bioRxiv - Immunology 2022Quote: Genomic DNA from sorted cell populations of greater than 200,000 cells was extracted using the DNeasy Kit (Qiagen). DNA from cell populations less than 200,000 cells was processed via direct lysis using DirectPCR lysis buffer (Viagen Biotech) ...
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bioRxiv - Cell Biology 2023Quote: ... cells (1 x 106 cells/well) were washed three times with PBS and lysed in Buffer RLT (Qiagen). Lysates were transferred to Eppendorf tubes on ice and stored at -80°C until ready for RNA purification ...
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bioRxiv - Evolutionary Biology 2024Quote: ... regius cells harboring cyanobacterial symbionts was subjected to independent genome amplification using REPLI-g Single Cell Kit (QIAGEN), and the amplified DNA samples were processed with S1 nuclease (TaKaRa ...
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bioRxiv - Physiology 2024Quote: ... The GFP+ and GFP− cells as remaining cells were either kept in QIAzol Lysis Reagent (QIAGEN, Valencia, CA), and stored at −80°C or immediately used for single cell analysis.
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bioRxiv - Cell Biology 2020Quote: ... for MDCK cells and Effectene (Qiagen # 301425) for A6 cells following the manufacturers’ instructions.
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with siRNAs (FlexiTube, Qiagen) using Lipofectamine RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were subjected to RBC lysis (Qiagen) according to the manufacturers protocol and used for the further steps described below ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were lysed with RLT buffer (Qiagen) and frozen at −80 for downstream RNA isolation ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell transfection was performed using Effectene (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed in QIAzol reagent (Qiagen) for RNA isolation or fixed with 4% paraformaldehyde (PFA ...
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bioRxiv - Genomics 2020Quote: ... Cells were then scraped and maxiprepped (Qiagen) with a maximum of 450 mg bacteria per column ...
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bioRxiv - Cell Biology 2019Quote: ... S2 cells were transfected using Effectene (Qiagen) accord to the instructions provided by the manufacturer.
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bioRxiv - Molecular Biology 2019Quote: ... cells were either collected into Qiazol (Qiagen) for RNA extraction or were cross-linked with 1 % formaldehyde (37 % ...
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bioRxiv - Cell Biology 2019Quote: ... cells were lysed in RLT buffer (QIAGEN) and RNA extracted using RNEasy extraction kit (Qiagen) ...
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bioRxiv - Molecular Biology 2019Quote: ... S2 cells were transfected using Effectene (Qiagen), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: MEF cells were transfected using HiPerFect (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Physiology 2019Quote: ... cells were lysed with Buffer RLT (Qiagen) containing 1 % 2-Mercaptoethanol and processed through a Qiashredder with total RNA extracted using the RNeasy isolation kit according to manufacturer’s instructions (Qiagen) ...
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bioRxiv - Biophysics 2021Quote: ... from cells homogenized on QIAshredder columns (Qiagen). RNA extracts were treated with RNase-free DNase (Qiagen) ...
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bioRxiv - Neuroscience 2020Quote: ... following manufacturer’s instructions for cell pellets (Qiagen). Genomic DNA concentrations were determined by photometry (Gen5 BioTek) ...
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bioRxiv - Molecular Biology 2020Quote: iAT2 cells were collected in Qiazol (Qiagen) or TRIzol (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: Cell lysates were homogenized using QIAshredder (Qiagen) and RNA was extracted using the RNeasy Plus Mini kit (Qiagen) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed using TCL buffer (Qiagen) and lysates were stored at -80 degrees ...
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bioRxiv - Microbiology 2021Quote: ... Cells were also harvested with QIAzol (QIAGEN) at 2 h after the HBSS wash and at 72 h post-infection for RNA extraction.
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bioRxiv - Genomics 2019Quote: Cells were stably transfected via Effectene (Qiagen) coupled with a piggyBac transposase integration system (Li et al. ...
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bioRxiv - Cell Biology 2019Quote: ... COS7 cells were transfected using SuperFect (Qiagen) and harvested after 38-42 h.
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bioRxiv - Immunology 2019Quote: ... Cells were resuspended in RLT buffer (Qiagen) and vortexed for 1 minute before being placed at −80°C ...