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Citations for Qiagen :
851 - 900 of 5525 citations for Primary Human Brain Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Genomic DNA was extracted from puromycin selected SUDHL4 cells and untreated WT cells using the QIAamp DNA Blood Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Purified CD4+FYP+ Treg cells were stimulated with Cell Stimulation Cocktail (eBioscience) for 2 hrs and lysed in RLT buffer (Qiagen) containing 1% 2-mercaptoethanol (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... the cell culture supernatants were collected and cells were lysed using RLT Buffer from the RNeasy Mini Kit (#74106; Qiagen).
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was isolated from 1 million cells of each cell line using the QIAamp®DNA Micro Kit (QIAGEN) following the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2023Quote: ... Mitochondria isolation from HEK293T cells was performed after 24 hours of cell transfection using the Q proteome Mitochondria Isolation Kit (QIAGEN). Cell lysis and mitochondria homogenisation were conducted as per the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... RNA from CD4+ T cells (2×106 cells per condition) was extracted by using the RNeasy Plus Mini kit (QIAGEN). Extracted total RNA was quantified using the Qubit broad range RNA assay ...
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bioRxiv - Developmental Biology 2023Quote: ... we used 6 mL of 55 °C Cell Lysis Solution from The Gentra Puregene® Cell and Tissue Kit (Qiagen) with 0.1 mg/mL proteinase K and 1% β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2023Quote: Bulk-RNA free of genomic DNA for RT-qPCR analysis was extracted from cell pellets (≤ 2 x 106 cells) using the RNeasy plus spin-column purification kit (Qiagen) and performed using the Qiacube liquid handler (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA from Library-Diverse was isolated by Blood & Cell Culture DNA Maxi Kit (Qiagen; 30 million cells per condition). Genomic DNA from the TRIP library editing experiments was isolated by DNeasy Blood & Tissue Kit (4 million cells per condition) ...
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bioRxiv - Bioengineering 2023Quote: ... phenol-free total RNA was extracted from cells directly in cell culture wells using RNeasy Plus Micro Kit with gDNA eliminator columns (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... of either the B cell clone or polyclonal B cells of the patients were directly FACS sorted into 100µL ALT lysis buffer (Qiagen MicroKit). gDNA was extracted as per manufacturer’s instructions (Qiagen MicroKit) ...
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bioRxiv - Molecular Biology 2023Quote: Cells collected from either 3D culture organoids or sorted cells were lysed in RLT buffer from RNeasy kit (Qiagen, 74106). RNA samples were isolated using the same kit following the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: Genomic DNA from ex vivo isolated CD8+ T cell subsets or in vitro cultured cells was extracted using the DNeasy Blood & Tissue Kits (Qiagen) and subsequently bisulfite-converted by using the EZ DNA Methylation-Lightning Kit (Zymo Research ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA Extraction and Purification: Genomic DNA was extracted from 5 M cells using the Gentra Puregene Cell Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: Genomic DNA from freshly sorted single cells was extracted and amplified using the REPLI-g Advanced Single Cell kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: The DNA was extracted from the FACS sorted cells and whole genomes were amplified using multiple displacement amplification technique (MDA) with the REPLI-g Single Cell kit (Qiagen) by following the manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... genomic DNA was extracted from cell pellets containing at least 1e6 cells/pellet using the DNeasy Blood and tissue kit (Qiagen), followed by bisulfite conversion using the Epitech Fast Bisulfite Conversion Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2021Quote: ... AllStars Hs Cell Death siRNA (Cat# 1027298, Qiagen) was used as a positive control for transfection efficiency ...
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bioRxiv - Genetics 2021Quote: ... S2R+ cells were transfected using Effectene (Qiagen, 301427) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Sorted cells were collected in RLT buffer (Qiagen) and frozen at -80°C.
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: ... (AllStars Hs Cell Death Control siRNA from Qiagen), and siRNA against IKKγ ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed using RNeasy lysis buffer (QIAGEN) supplemented with DTT and proteins were degraded using proteinase K (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were either harvested for DNA extraction (Qiagen) or frozen down for long-term storage ...
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bioRxiv - Immunology 2020Quote: ... cells were suspended in RLT buffer (Qiagen®) before freezing at -80°C until analysis ...
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bioRxiv - Immunology 2022Quote: ... cells were lysed (RLT buffer, Qiagen, Valencia, California) and RNA was extracted (RNeasy Plus mini kit ...
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bioRxiv - Developmental Biology 2020Quote: ... Sorted cells were collected in RLT buffer (Qiagen) for RNA isolation.
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bioRxiv - Cancer Biology 2019Quote: A375 cells were stability transfected via Effectene (Qiagen) coupled with a piggyBac transposase integration system (43 ...
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bioRxiv - Developmental Biology 2019Quote: ... sorted cells were homogenized with a QIAshredder (QIAGEN)and total RNA extracted using the RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Systems Biology 2019Quote: ... cells were lysed using Buffer RLT Plus (Qiagen) with 1% beta-marcaptoethanol (Sigma) ...
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bioRxiv - Pathology 2019Quote: ... Fresh cells were processed through Qiashredder (Qiagen 79654) and RNeasy mini kit (Qiagen 74104 ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cell cultures were treated with RNA protect (Qiagen) to stabilise RNA ...
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bioRxiv - Cell Biology 2019Quote: ... Remaining cells were resuspended in RLT buffer (Qiagen). RNA was isolated by RNeasy Micro or RNeasy Mini kits (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... sorted cells were lysed in TCL buffer (Qiagen). After purification of nucleic acids by Agencourt RNA Clean XP (Beckman Coulter ...
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bioRxiv - Genomics 2020Quote: ... cells were mixed 1:2 with RNAProtect (Qiagen) and harvested via centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed with Buffer RLT (Qiagen, USA) containing 0.01% β-mercaptoethanol for RNA analysis ...
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bioRxiv - Biochemistry 2020Quote: Cells were transfected with plasmids using Effectene (Qiagen). For the transfection ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected by lipofection using Effectene (Qiagen) 1 day prior to experiments ...
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bioRxiv - Cancer Biology 2020Quote: DNA from cells were extracted using DNeasy (QIAgen). DNA (100 ng ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed with 1X TCL buffer (Qiagen) supplemented with 1% beta-mercaptoethanol ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with plasmids using Effectene (Qiagen) and with siRNA using HiPerFect (Qiagen) ...
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bioRxiv - Cell Biology 2021Quote: Cells were transfected with siRNA using HiPerfect (Qiagen). We used 20 nM KDM4A FlexiTube GeneSolution siRNA (Qiagen ...
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bioRxiv - Physiology 2020Quote: ... BeWo cells were lysed using RLT buffer (Qiagen) and RNA extracted using the RNeasy mini kit (Qiagen ...
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bioRxiv - Systems Biology 2021Quote: RNA was extracted from cells using RNeasy (Qiagen). First strand cDNA was synthesized from 0.5-1 mg RNA by Superscript III First-Strand Synthesis superMix for qRT-PCR (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: The cells were lysed with QIAzol reagent (QIAGEN), and the total RNA was purified using a miReasy Mini Kit (QIAGEN ...
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bioRxiv - Cell Biology 2020Quote: ... stromal and Eph4 cells using RNeasy Kit (Qiagen). RNA underwent reverse-transcription with random primers (Invitrogen ...
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bioRxiv - Biophysics 2020Quote: ... Cells were homogenized with a QIAshredder (Qiagen #79654) with β-mercaptoethanol in the RTL buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... stabilized in RNAprotect cell reagent (Qiagen, Germantown, MD) and frozen for later mRNA isolation.
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed using Qiagen RLT buffer (Qiagen) supplemented with 1% β-mercaptoethanol (Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... Cells were homogenised with the QIAshredder kit (Qiagen) and total RNA was extracted from all samples using the RNeasy Plus Micro Kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... genomic DNA extraction from bacterial cells (DNEasy, QIAGEN), polymerase chain reaction (Q5 High-Fidelity 2x Master Mix and OneTaq ...