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Citations for Qiagen :
551 - 600 of 5525 citations for Primary Human Brain Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: Flag-tagged NF1-GRD (aminoacids 1131-1534) was amplified by PCR from human cortical tissue (epilepsy patient) and first cloned in the pDRIVE vector (Qiagen #231124). Primers are listed in Supplementary Table 5 ...
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bioRxiv - Immunology 2021Quote: ... and ImmunoCult™ Human Treg Differentiation Supplement (StemCell TechnoRNA was isolated at set time points after activation using the RNeasy Mini (Qiagen). cDNA was generated using the High-Capacity RNA-to-cDNA Kit (ThermoFisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... Exosomal miRNAs were profiled using Human Serum/Plasma miRCURY LNA miRNA PCR array (Qiagen, #YAHS-106Y, Plate Format: 2 × 96-well).
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bioRxiv - Microbiology 2023Quote: RNA was extracted from a SARS-CoV-2 Omicron BA.1 clinical isolate (SARS-CoV-2/human/USA/CA-CDC-4358237-001/2021) (GenBank: OM264909.1) using the QIAamp Viral RNA kit (Qiagen, Hilden, Germany). The complete wild-type Omicron genome was then reverse transcribed via RT-PCR with SuperScript™ IV First-Strand Synthesis System (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... 1 µg of RNA isolated from human islets or 10 µL of exosomal RNA was reverse transcribed using the miRScript II kit (Qiagen, Germany), following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The association of the differentially expressed proteins with specifically dysregulated regulatory/metabolic networks in OT human samples was analyzed using QIAGEN’s Ingenuity Pathway Analysis (IPA; QIAGEN Redwood City). This software calculates significance values (p-values ...
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Variation in Leishmania chemokine suppression driven by diversification of the GP63 virulence factorbioRxiv - Microbiology 2021Quote: ... Cells were resuspended in RNAprotect Cell Reagent (Qiagen) and stored at -80°C prior to extracting RNA with RNeasy RNA extraction kit (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were collected in RNAprotect Cell Reagent (Qiagen), and total RNA was extracted using the RNeasy Plus Micro kit (Qiagen) ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were then stained by Coomassie-blue or immunodetected as described before [26] using primary polyclonal antibodies directed against His6 epitope-tag (Penta His, Qiagen, dilution 1:1000), V5 epitope-tag (Bethyl Laboratories ...
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bioRxiv - Neuroscience 2019Quote: ... total RNA from magnetically purified human NDC, PSEN1M146L, and PSEN1A246E (replicates, n = 4) hiPSC-derived neurons was prepared using RNeasy Plus Micro Kit (Qiagen Cat. 74034) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: Total RNA from magnetically purified NDC, PSEN1M146L, and PSEN1A246E (replicates, n = 3) human iPSC-derived neurons was prepared using miRNeasy Micro Kit (Qiagen Cat. 217084), based on manufacturer’s procedures ...
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bioRxiv - Neuroscience 2019Quote: Total RNA from magnetically purified NDC, PSEN1M146L, PSEN1A246E, and PSEN1H163R (replicates, n = 3) human iPSC-derived neurons was prepared using RNeasy Plus Micro Kit (Qiagen, Cat. 74034) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... we extracted whole blood DNA from all individuals included in the RNA sequencing data using Gentra® Puregene® for human whole blood kit (QIAGEN) and MagAttract® HMW DNA kit (QIAGEN ...
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bioRxiv - Immunology 2020Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out by using the ‘Human Innate and Adaptive immune Response’ kit (Qiagen, Hilden, Germany) to assess the expression of genes/mRNA ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from 1 mL of each human plasma sample using the DNeasy Blood and Tissue kit (Qiagen, 69504, Hilden, Germany) according to the manufacturer protocol and recommendations ...
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bioRxiv - Microbiology 2023Quote: Microbiome DNAs were extracted from the human samples on the same day of collection using the microbiome-specific kit (QIAamp DNA Microbiome Kit; Qiagen, Hilden, Germany). The DNA extraction was performed according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Subsequent targeted sequencing of 160 cancer-related genes was performed using a QIAseq Human Comprehensive Cancer Panel v2 (Qiagen Inc, Valencia, CA) (Table S2) ...
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bioRxiv - Cell Biology 2023Quote: ... Key genes involved in the regulation and enzymatic pathways of fatty liver were simultaneously assayed with the RT2 Profiler PCR Array Human Fatty Liver (PAHS-157ZC-6) (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions and analyzed with the Data Analysis Center (QIAGEN Hilden ...
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bioRxiv - Molecular Biology 2023Quote: ... A total of 1 ng of RNA from microdissected human pancreatic islets and from EndoC-βH1 was used to generate cDNA libraries using QiaSeq miRNA library kit (Qiagen, Hilden, Germany) following manufacturer’s instructions (see ESM Methods).
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bioRxiv - Immunology 2019Quote: ... Red blood cells were lysed by treating the cells with Red Blood Cell lysis buffer (Qiagen) for 5 min ...
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bioRxiv - Immunology 2022Quote: RNA was isolated from Apoe-KO and Apoe/Has3-DKO aortae and primary bone marrow derived monocytes by using the RNeasy® Plus Universal Kit Mini 50 (Qiagen GmbH, Hilden, Germany). RNA concentration and purity (260 nm/ 280nm ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were FACS-sorted for mCherry+ cells directly into RNA-stabilizing cell lysis buffer (Qiagen Buffer RLT). Homogenization was performed on QiaShredder columns ...
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bioRxiv - Immunology 2022Quote: ... DNA was extracted from frozen cell pellets (3×107 cells; Blood & Cell Culture DNA Maxi Kit, Qiagen) per manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: Genomic DNA from cells in Qiagen Cell Lysis were purified using Qiagen Puregene Cell Kit (Qiagen 158722) according to manufacturer’s instructions and resuspended in 100µl nuclease-free H20 ...
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bioRxiv - Immunology 2020Quote: Cells were resuspended and stored in RNAprotect cell reagent (Qiagen). RNA was extracted with the RNeasy mini kit (Qiagen ...
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bioRxiv - Bioengineering 2023Quote: ... Single-cell suspensions were depleted of red blood cells (Qiagen) before downstream use.
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bioRxiv - Cancer Biology 2024Quote: ... To profile the expression of genes related to p53-mediated signal transduction based on the RT² Profiler™ PCR Array Human p53 Signaling Pathway (#330231, PAHS-027ZC, Qiagen, Hilden, Germany), reverse transcription and qPCR were performed using the RT2 First Strand Kit (#330401 ...
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bioRxiv - Molecular Biology 2022Quote: ... coli cells (Qiagen) to generate the protein expression strains ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells (Qiagen). The bacterial cultures were grown in LB medium with 100 μg/ml ampicillin and 50 μg/ml kanamycin at 37°C to an OD600 nm of 0.8 and expression was induced with 1 mM isopropylthio β-D-1-galactopyranoside (IPTG) ...
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bioRxiv - Immunology 2019Quote: ... Red blood cells were removed by treating the cells with 5 ml Red Blood Cell Lysis buffer (Qiagen) for 4 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell fractionation was performed with the Qproteome Cell Compartment kit (Qiagen).
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bioRxiv - Microbiology 2023Quote: Cell pellets were resuspended in 300 μL cell lysis solution (Qiagen) and lysed by incubation at 50°C for 10 min ...
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bioRxiv - Cell Biology 2021Quote: Isolated Tert+ and Tert-cells (≤ 5 cells) were subjected to synthesize the complementary DNA (cDNA) using REPLI-g WTA Single Cell Kit (QIAGEN) and analyzed for gene expression by qRT-PCR ...
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bioRxiv - Molecular Biology 2019Quote: ... confluent 293T-REx cells (25 cm2) were harvested and nuclei were isolated from 5×106 cells with the Qproteome Cell Compartment Kit (QIAGEN), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: 3 million cells from cell lines or patient samples were pelleted and resuspended in 1 mL of Cell Lysis Solution (Qiagen) mixed with 500 μg of RNase A ...
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bioRxiv - Microbiology 2020Quote: ... HIV-1 integration was examined in 293T cells and J-Lat cells by isolating genomic DNA from 1 × 106 cells using the DNeasy Tissue Kit (Qiagen). Alu-LTR sequences were amplified during first round PCR from 100 ng of total genomic DNA using the following primers ...
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bioRxiv - Immunology 2022Quote: ... for cell fractions ≥0.2×106 cells and the RNeasy Micro Kit (Qiagen) for fractions ≤0.2×106 cells ...
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bioRxiv - Neuroscience 2021Quote: ... Cell culture samples were collected with RNAprotect Cell Reagent (Qiagen, Germantown, MD) and the mRNA was extracted using a Qiagen RNeasy kit (Qiagen ...
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bioRxiv - Immunology 2019Quote: ... Red blood cells were lysed using Red Blood Cell Lysis buffer (Qiagen) for 1 min ...
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bioRxiv - Genetics 2020Quote: Cell pellets were resuspended in 600 ul Cell Lysis Solution (Qiagen, 158906) supplemented with 3 ul Proteinase K (NEB ...
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bioRxiv - Cell Biology 2023Quote: Cell samples were collected in RNAprotect Cell Reagent (Cat. No. 76526, QIAGEN) during the differentiation procedure at day 9 ...
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bioRxiv - Developmental Biology 2024Quote: ... GFP-positive cells were sorted into REPLI-g cell storage buffer (Qiagen).
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bioRxiv - Cell Biology 2020Quote: ... The relative expression of mRNAs of c-Myc regulated genes identified in RT2 Profiler™ PCR Array Human MYC Targets (Qiagen, Cat. no. PAHS-177Z) was determined by real-time quantitative RT-PCR ...
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bioRxiv - Microbiology 2020Quote: ... RNAprotect Cell Reagent (Qiagen) was added to stop the infection and preserve the RNA ...
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bioRxiv - Cell Biology 2022Quote: ... coli M15 cells (Qiagen) with pRAREII (Novagen).
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bioRxiv - Molecular Biology 2021Quote: ... coli M15 cells (Qiagen) using H2O-based M9 medium supplemented with 15NH4Cl and D-glucose-13C6-d7 ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA was extracted from ∼1 x 107 cells using a modified version of the Gentra Puregene Cell Kit cell extraction protocol (Qiagen 158745). Integrity of genomic DNA and absence of contaminating RNA was confirmed via 1% agarose 1x TAE gel electrophoresis ...
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bioRxiv - Neuroscience 2020Quote: ... pooled cells from each cell population using the RNeasy Plus Micro Kit (Qiagen) according to the manufacturers recommended protocol.
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bioRxiv - Molecular Biology 2019Quote: DNA from H1299 cells (using the Qiagen Blood and Cell Culture Midi Kit) was quantified using a NanoDrop DNA Spectrophotometer (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2019Quote: ... cells isolated by FACS or cultured cells using the RNEasy Mini kit (Qiagen) or the RNEasy Micro kit (Qiagen) ...