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Citations for Qiagen :
701 - 750 of 10000+ citations for Human Oligodendrocyte transcription factor 1 OLIG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... (underlined region is the T7 promoter for In vitro transcription) These primers amplified exonic region of Dyrk1b and the product was cleaned using PCR purification kit (Qiagen)and cloned into pGEM®-T Easy Vector Systems (Promega) ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was isolated from a pool of 20 mechanically homogenized embryos at 96 hpf for each sample (n=4) using TRItidy G (AppliChem) and 500 ng of cDNA was synthesized using QuantiTect Reverse Transcription Kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Equal amounts of total RNA from each samples were then reverse-transcribed into cDNA using QuantiTect Reverse Transcription Kit (QIAGEN). qRT-PCR was performed using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... genomic DNA was eliminated with a gDNA Wipeout Buffer and subsequently reverse transcribed into cDNA using the QuantiTect Reverse Transcription Kit (Qiagen). The diluted cDNA was prepared with SYBR Green (Roche) ...
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bioRxiv - Plant Biology 2022Quote: ... DNase treatment to remove contamination of genomic DNA and first-strand cDNA synthesis were performed using QuantiTect Reverse Transcription Kit (Qiagen). Quantitative real-time RT-PCR (qPCR ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA products were used as the template for quantitative reverse transcription-PCR (qRT-PCR) in technical triplicate using the QuantiNova SYBR Green PCR kit (Qiagen). mRNA levels were normalized to the geometric mean of RPL11 ...
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bioRxiv - Physiology 2023Quote: ... were normalized at 10 ng/µL and the reverse transcription of RNA was done following the manufacturer instructions (miRCURY LNA RT Kit, Qiagen). We added 0.5µl of controls UniSp6 and cel-mir-39-3p to the samples as internal reference to check the efficiency of the reverse transcription and PCR amplification ...
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bioRxiv - Immunology 2023Quote: ... cDNA was prepared from 2μg total RNA from each sample under the instruction of QuantiTech reverse transcription Kit (Qiagen, 205311). Samples for QPCR were then prepared under the instruction of ONEGreen fast qPCR kit (Ozyme ...
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bioRxiv - Cell Biology 2023Quote: ... gDNA wipeout buffer was used to remove Genomic DNA for 2 minutes at 42°C and cDNA was synthetized with the QuantiTect Reverse Transcription Kit (Qiagen). qPCR was performed with PowerUp SYBR Green Master Mix using the StepOnePlus Real-Time PCR system ...
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bioRxiv - Neuroscience 2023Quote: ... Two μL of isolated RNA were used for reverse transcription (RT) and cDNA synthesis using the miRCURY LNA RT Kit (Qiagen) according to the manufacturer’s instructions adding the RNA spike-in UniSp6 as quality control for RT ...
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bioRxiv - Biochemistry 2023Quote: The ribosomal DNA transcription assay was performed like described before.[44] Total RNA was extracted using the RNeasy kit from QIAGEN. cDNA was generated using Thermo Fisher Scientific SuperScript® III Reverse Transcriptase and 2 μg RNA following the vendor’s protocol ...
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bioRxiv - Immunology 2023Quote: ... 0.5 µg RNA was converted to cDNA using the Quantitect® Reverse Transcription Kit (Cat. No. 205311, Qiagen, Limburg, Netherlands). gDNA wipe-out buffer was added to RNA prior to the RNA conversion step ...
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Notch3 is a genetic modifier of NODAL signalling for patterning asymmetry during mouse heart loopingbioRxiv - Developmental Biology 2024Quote: Reverse transcription was performed on RNA isolated from entire (left and right) micro-dissected heart fields using a Reverse Transcription kit (QuantiTect, Qiagen). Quantitative PCR was carried out using the ViiA7 real-time PCR system ...
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bioRxiv - Genomics 2024Quote: ... Poly(A)RNA (500 ng) from each independent transfection was reverse transcribed to cDNA using QuantiTech Reverse Transcription kit (Qiagen) using a reporter-specific first strand primer (HJ7211 ...
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bioRxiv - Microbiology 2024Quote: ... reverse transcription quantitative PCR (RT-qPCR) assays were performed with the QuantiTect Virus + ROX Vial Kit (Qiagen, Toronto, ON, Canada) in a LightCycler® 480 system (Roche Molecular System) ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 1 μg of total RNA from each sample was used to obtain double strand cDNA with the QuantiTect Reverse Transcription Kit (Qiagen). Quantitative Real-Time PCR (qRT-PCR ...
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bioRxiv - Systems Biology 2020Quote: ... IL-8 ELISA was performed according to manufacturer instructions (Qiagen).
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bioRxiv - Microbiology 2023Quote: Microbiome DNAs were extracted from the human samples on the same day of collection using the microbiome-specific kit (QIAamp DNA Microbiome Kit; Qiagen, Hilden, Germany). The DNA extraction was performed according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... total RNA was extracted from tissues or cells as described above and reverse-transcribed using the QuantiTect Reverse Transcription Kit (Qiagen #205313). Quantitative real-time PCR (QRT-PCR ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was transcribed using reverse primer targeting the conserved termini of influenza gene segments and the Omniscript Reverse Transcription Kit (Qiagen, Germany). Each gene segment was amplified using segment-specific primers and extracted from 1% gel slices using Qiagen Gel Extraction Kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2020Quote: Up to 1 μg of RNA from each sample was converted into cDNA using a QuantiTect Reverse Transcription Kit and manufacturer’s directions (Qiagen, Hilden, Germany); a DNA-wipeout pre-step was included to avoid genomic contamination ...
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bioRxiv - Evolutionary Biology 2020Quote: ... First strand cDNA was reverse transcribed from 0.5 μg of total RNA using a Quantitect Reverse Transcription Kit (Qiagen, Hilden, Germany) that incorporates genomic DNA removal prior to reverse transcription ...
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bioRxiv - Genomics 2020Quote: RNA extracted (~1μg) from patient nasopharyngeal swabs was used for double stranded cDNA synthesis using the QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany) according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2019Quote: Genes were quantified by one-step quantitative reverse transcription-polymerase chain reaction (q-PCR) using the QuantiFast SYBR Green RT-PCR kit (Qiagen, UK). A master mix was prepared by mixing 6.25 μl SYBR green ...
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bioRxiv - Microbiology 2020Quote: ... 6 μl of each RNA sample were reverse transcribed using the Qiagen QuantiTect Reverse Transcription Kit according to the manufacturer’s instructions (Qiagen, Hilden, Germany). This protocol included an additional DNase treatment step and the reverse transcription reaction used a mix of random hexamers and oligo(dT ...
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bioRxiv - Neuroscience 2019Quote: ... Synthesis of cDNA was performed on 0.3-1.0 μg of normalized total RNA from each sample using QuantiTect Reverse Transcription kit (Cat No. 205313, Qiagen, Valencia, CA) which included a DNase treatment step to remove any residual genomic DNA contamination ...
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Global genetic patterns reveal host tropism versus cross-taxon transmission of bat BetacoronavirusesbioRxiv - Evolutionary Biology 2020Quote: ... All RNA extracts were subjected to reverse transcription polymerase chain reaction (RT-PCR) using the one-step RT-PCR kit (Qiagen, USA) and PanCoV F2 (5’-AAR TTY TAY GGH GGY TGG-3’ ...
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bioRxiv - Pathology 2022Quote: ... RNA concentrations were determined spectrophotometrically and 1µg of total RNA was reverse transcribed using the QuantiTect Reverse Transcription Kit (Qiagen, Valencia, CA). Real-time RT-PCR was performed using a StepOne real time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: 1 µg of RNA was used for cDNA synthesis using the QuantiTect Reverse Transcription Kit according to the manufacturers’ protocol (Qiagen 205313). Quantitative PCR was carried out on a RotorGeneQ (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... life technologies™) according to the manufacturer’s instructions before being reverse-transcribed using the QuantitTect® Reverse Transcription kit (Qiagen®). The resulting cDNA or serial dilutions of a known quantity of genomic DNA for generation of standard curves were amplified using a QuantiFast® SYBR® Green PCR kit (Qiagen® ...
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bioRxiv - Pathology 2020Quote: ... Five hundred ng of RNA was reverse transcribed to cDNA using a reverse transcription RT2 First Strand Kit (Qiagen, Hilden, Germany) that is recommended to use in combination with RT2 Profiler PCR Arrays and we followed manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... an equal volume of RNA per reaction was DNase-treated and reverse transcribed using the QuantiTect Reverse Transcription Kit (Qiagen, 205314).
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bioRxiv - Genetics 2020Quote: ... The RNA pellet was eluted in 15 μL of nuclease-free water and quantified on a Nanodrop 1000 spectrophotometer (NanoDrop Technologies) prior to DNAse treatment with QuantiTect® Reverse Transcription kit (Qiagen). The quality of the RNA was examined by electrophoresis on a 1% agarose gel (Sigma Aldrich) ...
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bioRxiv - Physiology 2020Quote: ... One μg of total RNA was reverse transcribed into cDNA using the Qiagen QuantiTect Reverse Transcription Kit (Qiagen, Valencia, California, USA) following manufacturer’s protocols ...
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bioRxiv - Genomics 2022Quote: ... The RNA pellet was eluted in 15 μL of nuclease-free water and quantified on a Nanodrop 1000 spectrophotometer (NanoDrop Technologies) prior to DNAse treatment with QuantiTect® Reverse Transcription kit (Qiagen). The quality of the RNA was examined by electrophoresis on a 1% agarose gel (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2022Quote: PCR products produced as templates for in vitro transcription were purified with either the Qiaquick PCR Purification Kit (Qiagen, Hilden, Germany) for Cas9 templates ...
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bioRxiv - Neuroscience 2022Quote: ... Complementary DNA (cDNA) was subsequently generated using 2 μg of total RNA and the miScript II Reverse Transcription (RT) Kit (Qiagen, Australia), according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... RNA concentrations were determined spectrophotometrically and 1µg of total RNA was reverse transcribed using the QuantiTect Reverse Transcription Kit (Qiagen,Valencia, CA). Real-time RT-PCR was performed using a StepOne real time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The quality and quantity of the isolated total RNA were measured using a spectrophotometer and synthesised the cDNA using QuantiTect Reverse Transcription Kit (QIAGEN, Germany). Synthesized cDNA was kept at -80°C till further use.
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bioRxiv - Microbiology 2023Quote: ... RNA was normalised to the lowest sample concentration and cDNA libraries prepared using the QuantiTect® Reverse Transcription Kit (QIAGEN, UK) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 500ng total RNA were transcribed into complementary DNA using oligo-dT primers and OmniScript® Reverse Transcription kit (QIAGEN, Hilden, Germany). Transcription levels of genes of interest were assessed by quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2024Quote: ... and real-time reverse transcription quantitative PCR (qRT-PCR) was performed using QuantiNova™ SYBR Green PCR Kit (Qiagen, Hilden, Germany). Primers were synthesized by IDT Inc ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA from human and mouse kidney samples was harvested using the RNeasy Mini Kit (Qiagen). Total RNA isolation from cultured cells was extracted using Trizol reagent (Ambion ...
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bioRxiv - Immunology 2021Quote: Genomic DNA was prepared from human or mouse B cells using QIAmp DNA Mini Kit (Qiagen), or from paraffin-embedded human IgD or IgA myeloma tissue sections (obtained from the University of Arkansas for Medical Science ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA from human cancer cells was extracted 48h post transfection using the RNeasy Kit from Qiagen according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: Total RNA was isolated from murine and human monocytes with the RNeasy Plus Micro Kit (Qiagen), and then 200 ng of RNA was reverse transcribed using the iScript RT Supermix (Bio-Rad) ...
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bioRxiv - Genomics 2022Quote: ... cfDNA was then extracted from human blood plasma using the QIAmp Circulating Nucleic Acids kit (Qiagen), eluted in 60-μl elution buffer (10 mM Tris-Cl ...
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bioRxiv - Immunology 2020Quote: Total RNA was extracted from mouse or human whole blood using RNA blood mini kit (Qiagen). Isolated RNA was converted to complementary DNA (cDNA ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from cultured human and mouse cells using an RNeasy Mini Kit (Qiagen) and included an on-column DNase treatment to eliminate contaminating genomic DNA ...
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bioRxiv - Immunology 2022Quote: ... RNA was extracted from 5×106 human PBMCs from Cohort II using AllPrep RNEasy kits (Qiagen) and the small RNA-containing column flow-through collected ...