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Citations for Qiagen :
601 - 650 of 10000+ citations for Human Oligodendrocyte transcription factor 1 OLIG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Human rRNA depletion was performed using the QIASeq FastSelect kit (Qiagen, Hilden, Germany). RNA sequencing library preparation used NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s recommendations (New England Biolabs ...
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bioRxiv - Immunology 2023Quote: DNA from human stool samples was extracted with DNeasy PowerSoil Pro kit (Qiagen) according to the manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2022Quote: Total RNA for human TSCs was extracted using RNeasy Micro kit (Qiagen, 74004) with on-column DNase digestion ...
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bioRxiv - Synthetic Biology 2023Quote: ... or primary human cells were extracted using the Qiagen RNeasy Micro Kit (Qiagen). cDNA was produced using the SuperScript IV VILO cDNA Synthesis Kit (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... After 24 hours the media was removed for ELISA and the cells were lysed and RNA extracted (RNeasy Mini Kit, Qiagen).
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bioRxiv - Molecular Biology 2020Quote: ... Messenger RNA (mRNA) cDNA was synthesized using the QuantiTect® Reverse Transcription kit (Qiagen Inc., Germantown, MD, # 205313). Intron-spanning primers for mRNA were used to eliminate amplicons generated from any contaminating genomic DNA ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA of 500 ng was used for preparing cDNA libraries using QuantiTect Reverse Transcription Kit (Qiagen, USA). Gene expression was estimated by QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Plant Biology 2020Quote: ... DNase-treated RNA (2μg) was then reverse-transcribed in a total volume of 20μl using the Omniscript Reverse Transcription Kit (Qiagen).
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bioRxiv - Immunology 2021Quote: ... Five hundred nanograms of RNA was reverse transcribed into cDNA using the QuantiTect reverse transcription kit (Qiagen, # 205311). For one real-time reaction ...
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bioRxiv - Physiology 2021Quote: ... RNA isolation and reverse transcription: total RNA was extracted following the manufacturer’s recommended protocol (RNeasy Mini Kit, Qiagen). The quality and integrity of the total RNA were quantified by the NanoDrop DeNovix ...
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bioRxiv - Neuroscience 2022Quote: The reverse transcription was performed with 500 ng of total RNA using the miScript RT II kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... Reverse transcription of small RNAs was performed for all embryonic stages using the miRCURY LNA RT kit (Qiagen) using the recommended 10 ng total RNA input.
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bioRxiv - Molecular Biology 2020Quote: ... complementary DNA strand was synthesized from one μg of total RNA using QuantiTect® reverse transcription kit (Qiagen). Quantitative RT-PCR gene amplification was carried out using the CFX-96 thermocycler (Bio-Rad ...
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bioRxiv - Developmental Biology 2019Quote: ... The gRNA template for in vitro transcription was PCR amplified and purified with Qiaquick PCR purification Kit (Qiagen). In vitro transcription followed and was performed as described52 ...
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bioRxiv - Physiology 2019Quote: RNA was extracted from tissues using QIAzol and cDNA was synthesized using the Quantitect Reverse Transcription Kit (Qiagen). qPCR was conducted using Quantitect Sybr Green qPCR (Qiagen ...
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bioRxiv - Physiology 2021Quote: ... Complementary DNA (cDNA) synthesis was performed with 200–1,000 ng total RNA using QuantiTect_ Reverse Transcription Kit (Qiagen). Quantitative real time polymerase chain reaction (qRT–PCR ...
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bioRxiv - Microbiology 2020Quote: ... RNA (100 ng) was analyzed by reverse transcription-PCR (RT-PCR) using the OneStep RT-PCR kit (Qiagen). RNA was reverse transcribed and amplified in a total volume of 50 μl containing 2.5 mM MgCl2 ...
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bioRxiv - Microbiology 2020Quote: ... cDNA synthesis was performed from total RNA following the manufacturer’s instructions for the QuantiTect Reverse Transcription kit (QIAGEN). Gene silencing efficiency was determined using the SYBR Premix Ex Taq kit (Takara ...
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bioRxiv - Neuroscience 2022Quote: ... 500ng of RNA was used per biological replicate for cDNA conversion using QuantiTect reverse transcription kit (Qiagen, 205311). qPCR was run on a BioRad CFX96 using iTaq with SYBR Green (BioRad ...
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bioRxiv - Physiology 2022Quote: ... RNA (2000 ng) was reverse transcribed to cDNA using the QuantiTect Reverse Transcription Kit (Cat No. 205311, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... 500 ng of total RNA was used to synthesize first-strand cDNA using the Reverse Transcription Kit (Qiagen). For lncRNA expression profiling ...
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bioRxiv - Bioengineering 2023Quote: ... direct RT-qPCRs were performed on heat-inactivated samples without extraction using the QuantiTect Reverse Transcription Kit (Qiagen). The reactions were run in an Applied Biosystems QuantStudio 7 Flex System (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Complementary DNA (cDNA) was synthesized from 500 ng DNAse-treated RNA using the QuantiTect reverse transcription kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715, human YTHDF2: QIAGEN SI00764757, human YTHDF3: QIAGEN SI04133339 ...
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bioRxiv - Biochemistry 2023Quote: ... Co-factors were purified using Ni-NTA agarose beads (Qiagen), eluting with 300 mM imidazole ...
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bioRxiv - Neuroscience 2021Quote: ... or postmortem human cerebellar vermis samples using either the RNeasy Kit or RNeasy Fibrous Tissue Kit following the manufacturer’s protocols (QIAGEN). For tissue RNA extraction ...
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bioRxiv - Genetics 2021Quote: Human genomic DNA (gDNA) was extracted using the QIAamp DNA Mini Kit (QIAGEN, Germany) and quantified using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: RNA was extracted from 2×106 human PMNs using the RNeasy Mini Kit (Qiagen) as per manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... Human RAC2 3’UTR was amplified with One Step Ahead RT-PCR kit (Qiagen) from human mRNA using the following primers RAC2 3’UTR+ ...
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bioRxiv - Immunology 2021Quote: ... total RNA of CD8+ human T cells was extracted by miRNeasy Mini Kit (Qiagen), following the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and human islets were isolated using RNeasy Mini or Micro kits (Qiagen; Valencia, CA). Reverse transcription was completed with a High Capacity cDNA Reverse Transcription kit (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... rodent tissues and human samples using the Qiagen Blood and Tissue Kit (Qiagen, USA). The procedures prior to protein digestion were as follows ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA from cultured human cells were isolated using the QIAamp DNA kit (Qiagen) and genomic DNA from MEFs and mouse tissue samples were extracted using the DNeasy Blood and Tissue Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated from human cells with an RNA purification kit (Qiagen, 74134) and genomic DNA was removed by genomic DNA binding columns in the kit ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Total RNA was extracted from cultured human ECs using a RNeasy Mini kit (Qiagen). For reverse transcription ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... or human ABCB1-transfected MDR-19 cells using the RNeasy Mini extraction kit (Qiagen). First strand cDNA synthesis was performed on 500 ng of template RNA using MMLV reverse transcriptase (fc 10 units/µl) ...
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bioRxiv - Physiology 2023Quote: Total RNA was isolated from human islets using the miRNeasy Mini Kit (Qiagen, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from human cells using RNeasy Mini kits (QIAGEN, Hilden, Germany) and reverse transcribed using Super-Script III (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... genomic DNA from human primary T cells was isolated using Gentra Puregene Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... A total of 78 serum samples (positive for HEV IgM in ELISA) were subjected to RNA extraction using QIAamp Viral RNA Mini Kit (Qiagen, Germany). Using SuperScript™ III One-Step RT-PCR System with Platinum™ Taq (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... 80–90% depletion of OGT/OGA mRNAs was verified by RT-PCR using QuantiTect Reverse Transcription Kit (205311, Qiagen), QuantiTect SYBR Green PCR Kit (204143 ...
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bioRxiv - Evolutionary Biology 2020Quote: Reverse transcription (RT) of miRNAs was carried out using miRCURY LNA Universal RT microRNA PCR Kit (Exiqon-Qiagen, Denmark), as instructed in miRCURY LNA RT Kit manual in three technical replicates for each miRNA ...
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bioRxiv - Developmental Biology 2021Quote: ... Reverse transcription and PCR were carried out according to the manufacturer’s descriptions using the HotStarTaq PLUS DNA Polymerase kit (Qiagen) on a PTC-200 Peltier Thermal Cycler (MS Research) ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA synthesis for two-step RT-PCR was performed using the QuantiTect Reverse Transcription Kit (cat 205313) from QIAGEN. For each sample ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription-PCR (RT-PCR) was carried out with total RNA using the one-step RT-PCR kit (QIAGEN) according to the supplier’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: Transcription activity of EGR1 was measured using the dual luciferase assay with the Cignal EGR1 Reporter Assay Kit (Qiagen). MIR2052HG knocked-down MCF7/AC1 and CAMA-1 cells were transfected with either EGR1 reporter (EGR1-responsive GFP reporter) ...
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bioRxiv - Plant Biology 2019Quote: ... Freshly extracted mRNA from 10 aphids was converted into cDNA using the QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany) and 40 ng of cDNA was used as the template for qRT-PCR in an Applied Biosystems 7500 FAST real-time PCR system ...
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bioRxiv - Cell Biology 2021Quote: ... 200 to 500 ng of RNA were used to prepare cDNA using the QuantiTect Reverse Transcription Kit (Qiagen, 205311) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Equal amounts of RNA samples were used for cDNA synthesis by QuantiTect reverse transcription (RT) kit according to the manufacturer’s instructions (Qiagen). qPCR was performed using the following primers ...
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bioRxiv - Neuroscience 2021Quote: ... RNA amount was measured with the GeneQuant 1300 and transcribed into cDNA using the QuantiTect Reverse Transcription Kit (Qiagen). qPCR was performed with the KAPA SYBR FAST Universal Kit (Peqlab ...