Labshake search
Citations for Qiagen :
701 - 750 of 773 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: DNA was extracted from the collected leaf samples of the most relatively resistant and susceptible accessions using a modified DNeasy PowerSoil Pro kit (Qiagen, CA, USA) protocol ...
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bioRxiv - Neuroscience 2024Quote: Microbial sequencing was performed as previously described in.21,22 Microbial DNA was extracted from 50∼100 mg fecal material using the Qiagen DNeasy PowerSoil Pro Kit and the automated QIAcube Connect robot (Qiagen, Carlsbad CA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... These leftover materials were collected by LCM and placed into 350 μl of RLT lysis buffer (RNeasy, Qiagen, including 1%β-Mercaptoethanol (β-Me)) followed by RNA extraction and examination with the 2100 Bioanalyzer.
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bioRxiv - Plant Biology 2020Quote: ... His-tagged CLEL6 precursor was purified from bacterial extracts by metal chelate affinity chromatography on Ni-NTA Agarose (Qiagen, Hilden, Germany) according to the manufacturer’s recommendations ...
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bioRxiv - Synthetic Biology 2021Quote: ... Clarified supernatants containing TtgR and SmtB were purified using His-tag affinity chromatography with a gravity column charged with Ni-NTA Agarose (Qiagen #30210). The eluted fractions from the FPLC (for TetR ...
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bioRxiv - Synthetic Biology 2020Quote: ... For SARS-CoV-2 antigen detection the blots were incubated with 1:2000 Anti-His mouse monoclonal primary antibody (Qiagen, #34660) and then incubated with 1:10000 peroxidase labelled anti-mouse IgG secondary antibody (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The coverslips were then incubated for ~ 10 min with 4 μg/ml of Penta•His biotin conjugate antibody (34440, Qiagen, UK) in reaction buffer [40 mM HEPES buffer (pH 7.3 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Clarified supernatant containing mRFP1 was then purified using His-tag affinity chromatography with a gravity column charged with Ni-NTA Agarose (Qiagen #30210). The elution from the gravity column was concentrated and buffer exchanged (25 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: Nunc MaxiSorp™ 96-well plates were coated with 75 ng/well of penta-His antibody in PBS solution (Qiagen, 34660) and incubated overnight at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... HIS-fused p300 was expressed from pFastBac1 vector in High-Five cells and purified on Ni-NTA agarose beads (Qiagen, 30210) in BC buffer (20 mM HEPES pH7.9 ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were clarified by additional centrifugation at 14,000 xg for 30 minutes and supernatants were incubated 1 hour at 4 °C with 5 mL of His-Pur™ Ni-NTA resin (Qiagen) previously washed 3 times with 25 mL of Lysis Buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... followed by incubations with a PBS-based protein binding mixture for 1 h and with Alexa488-conjugated anti-His antibody (1:20 dilution, Qiagen 35310) for 1 h ...
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bioRxiv - Biochemistry 2022Quote: Lysates of His-tagged α-catenin WT and Δmod were bound to Ni-NTA (Ni2+-nitrilotriacetic acid)-sepharose affinity chromatography (Qiagen), washed with 50 mM Tris pH 7.8 ...
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bioRxiv - Cell Biology 2023Quote: Total RNA from 1,000 H3K27me3 HI/LO β-cells or from 50,000 sorted βHI and βLO cells was extracted using the miRNeasy FFPE Kit (QIAGEN, 217504), followed by the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (E6420L) ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane was incubated for 1 hour with monoclonal α-His antibodies conjugated to the horseradish peroxidase (1:5000 dilution; Qiagen). Blots were visualized with the addition of Lumina Forte Western HRP Substrate (MilliporeSigma ...
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bioRxiv - Immunology 2022Quote: Total DNA was extracted from 400ul of BAL fluid or swabs (fecal/oral/nasal) using the DNeasy Powersoil Pro Kit (Qiagen, Valencia, CA, USA). The hypervariable V4-V5 region of the 16S rRNA gene was amplified using PCR primers (515F/806R with the forward primers including a 12-bp barcode ...
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bioRxiv - Developmental Biology 2022Quote: Microbial genomic DNA was extracted from the pigs colonic digesta samples and the sows faeces using a QIAamp PowerFecal Pro DNA stool kit (Qiagen, West Sussex, UK) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was extracted from fecal material (0.25 g wet weight) using the QIAamp Power Fecal Pro DNA kit (QIAGEN GmbH, Cat. No. 51804) and was quantified using Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Total DNA was extracted from 50.0 mg of fresh ground tissue using the DNeasy® Plant Pro® Kit (Qiagen, Germantown, Maryland, USA), following manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... then gently scraping the cells of the filters with inoculation loops and placing the resuspended cells into the first tube of the DNeasy PowerSoil Pro Kit (Cat. No. 47016, Qiagen, Germantown, MD, USA) containing beads then following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: 109 conidia of the isolate Pt2 were subjected to gDNA extraction by using the PowerPlant® Pro DNA Isolation Kit (Qiagen, Hilden, Germany) (the manufacturer’s instructions were followed) ...
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bioRxiv - Plant Biology 2024Quote: ... DNA was extracted from bulk soil combined from multiple plants and extracted using Qiagen DNeasy 96 PowerSoil Pro kits following all manufacturer’s instructions (Qiagen, Hilden, Germany; catNo: 47017). All samples were then prepared by Novogene (Novogene Corporation Inc. ...
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bioRxiv - Plant Biology 2024Quote: ... Approximately 0.1 g of the resulting powder was added to Qiagen DNeasy 96 PowerSoil Pro kits and followed all manufacturer’s instructions to extract DNA (Qiagen, Hilden, Germany; catNo: 47017).
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bioRxiv - Physiology 2024Quote: Ileal samples were weighed and whole genomic DNA was extracted using the PowerFecal Pro® DNA Isolation Kit as per the manufacturer’s instructions (Qiagen, Germantown, MD, USA). 1 μl of each sample was used for 2-step real-time PCR amplification using SFB-specific primers (SFB736F ...
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bioRxiv - Genetics 2024Quote: ... 800 μL of the sample lysate was collected and transferred into the provided PowerBead Pro Tube and mechanically lysed using PowerLyzer® 24 Homogenizer (110/220 V) (Catalog number 13155, QIAGEN, Hilden, Germany) at 3000 rpm for 30 seconds ...
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bioRxiv - Plant Biology 2024Quote: ... DNA was purified from rhizosphere extracts and bulk soil using a QIAGEN DNeasy PowerSoil Pro DNA Isolation Kit (QIAGEN Inc., Germantown, MD, USA). Purified DNA was submitted to Integrated Microbiome Resource (Dalhousie University ...
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bioRxiv - Microbiology 2024Quote: Total DNA was extracted from catheter and urine pellets obtained in the previous step using DNeasy PowerSoil Pro Kit (Qiagen, cat. no. 47016) according to the manufacturer’s instructions except that the final elution was in 50 µL of water ...
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bioRxiv - Microbiology 2024Quote: Bacterial pellets were thawed at room temperature and DNA was extracted using a commercially available extraction kit (DNeasy® PowerSoil ® Pro Kit, QIAGEN, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... total RNA was extracted using RNeasy kit (QIAGEN, D usseldorf, Germany) from between 5 ×106 and 10×106 Z-138 ...
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bioRxiv - Bioengineering 2020Quote: ... The RBD wildtype and mutant proteins of SARS-CoV-2 spike with 10×his tag at N terminal were expressed in HEK 293 and purified with Ni-NTA affinity columns (Qiagen, Hilden, Germany).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed at indicated time points after warming and then processed for immuno-identification of HPK using an antibody that recognizes the polyhistidine tag (RGS-His antibody; Qiagen 1:100) and counterstained for nuclei using DAPI ...
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bioRxiv - Plant Biology 2020Quote: ... His-tagged PSK1 or RGF1 precursors were purified from bacterial extracts by metal chelate affinity chromatography on NiNTA Agarose (Qiagen, Hilden, Germany) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: The recombinant His-TvFACPα full-length protein was produced and purified by a standard protocol as suggested by the supplier (QIAGEN, Hilden, Germany) (48 ...
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bioRxiv - Developmental Biology 2020Quote: ... and unfragmented genomic DNA (A260/A280 ≥ 1.8 and A260/A230 ≥ 1.9) was extracted from whole blood obtained from the subject and his parents using the Puregene Blood kit from Qiagen (Valencia, CA). Whole exome sequencing was performed using the service provided by Beijing Genomics Institute (Cambridge ...
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bioRxiv - Cell Biology 2023Quote: ... His-tag fusions were purified by incubating the bacterial soluble fraction with pre-equilibrated Ni-NTA agarose bead slurry (Qiagen, Hilden, Germany) for 1 hour at 4°C and eluting with 500 mM imidazole ...
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bioRxiv - Immunology 2022Quote: ... Total DNA from feces was isolated using the QiaAmp® PowerFecal® Pro DNA Kit on the QIAcube Connect device (Qiagen, Germantown, MD, USA). For 16S metagenomic analysis ...
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bioRxiv - Microbiology 2022Quote: Bacterial DNA in the mouse fecal samples and GI content (200 mg) was extracted using QIAamp® PowerFecal® Pro DNA kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The sorting was conducted on a BD Aria III directly into a 350µL lysis buffer (Buffer RLT plus, supplemented with 10µL β-ME per 1mL Buffer RLT plus from the QiaGen RNeasy Plus Micro Kit, Cat. No. 74034) and subsequently stored at -80 oC for future batch RNA extraction.
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bioRxiv - Biochemistry 2021Quote: ... Samples were incubated at 95°C for 5 minutes and spun down at 17K x g for 10 min to remove cell debris before analysis of raw supernatant was performed via SDS-PAGE using a 12% acrylamide-tris gel and subsequent overnight transfer to a Western blot PVDF membrane and visualization with an anti-His antibody (Qiagen Cat# 34440, RRID:AB_2714179).
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bioRxiv - Microbiology 2021Quote: ... coli BL21 (DE3) and expression of the His-tagged Cas proteins was performed following the instruction of the protein purification kit (Qiagen, Valencia, CA, USA). Single colonies of transformed cells were cultivated overnight ...
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bioRxiv - Genomics 2024Quote: ... Then the total volume was spun down and plasmid pools were extracted from the bacterial pellets using 2-3 columns of the Hi-Speed Plasmid Maxiprep kit (Qiagen, catalog no. 12663) per sublibrary ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted from nine samples from a variety of mammal species using the QIAmp® PowerFecal® Pro DNA Kit (QIAGEN®, Hilden, Germany). DNA extracts were quantified using a Qubit 4 fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... DNA extractions using higher sediment volumes (up to 10 grams) with Qiagen DNeasy PowerMax Soil led also to low DNA yields (similar ranges as those reported for Qiagen DNAeasy Powersoil Pro kit tests). Because higher volumes of sediment also increase the concentrations of inhibitory compounds found in Guaymas subsurface sediments ...
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bioRxiv - Microbiology 2024Quote: ... A 100 mg portion was taken from each fecal sample to extract genomic DNA using the DNeasy PowerSoil Pro Kit from Qiagen (Qiagen, Palo Alto, CA, USA). Subsequently ...
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bioRxiv - Developmental Biology 2020Quote: ... and 1 μM Locked Nucleic Acid (LNA) oligo-d(T)30 (Qiagen) in primary-probe hybridization buffer composed of 40% formamide (Sigma ...
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bioRxiv - Cell Biology 2024Quote: Cells were washed once with D-PBS and lysed using QIAzol reagent (QIAGEN). Total RNA was extracted using the Direct-zol RNA Miniprep kit (Zymo Research) ...
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bioRxiv - Immunology 2021Quote: ... D from four matched BM samples using the RNeasy Mini Kit (Qiagen, Inc. Valencia, CA) by following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted combining a lysozyme method and the D-neasy blood tissue kit (Qiagen). The product was purified by the PCR purification kit (Qiagen ...
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bioRxiv - Plant Biology 2022Quote: ... and 200 µM VLCFA mix for 1 d using the RNeasy Plant kit (QIAGEN, Hilden, Germany). For isolation of RNA from the bending site of roots ...
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bioRxiv - Immunology 2024Quote: ... according to the method described in appendix D of the Qiagen RNeasy mini kit manual (Qiagen, 74104). RNA concentrations were determined using a Nanodrop one instrument (Thermofisher Scientific).