Labshake search
Citations for Qiagen :
451 - 500 of 773 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... 100 μl of each DNA sample were further processed using the DNeasy PowerClean Pro Cleanup kit (QIAGEN), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: DNA was extracted from cambium tissue using Qiagen’s DNeasy Plant Pro Kit (catalog # 69204, Qiagen, Hilden, Germany) following the manufacturer’s protocol with the addition of 100 μl of the Solution PS per sample due to high amounts of phenolic compounds in pine species ...
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bioRxiv - Microbiology 2022Quote: Total DNA was extracted from rectal swabs using the DNeasy Powersoil Pro Kit (Qiagen, Valencia, CA, USA). The hypervariable V4-V5 region of the 16S rRNA gene was amplified using PCR primers (515F/926R with the forward primers including a 12- bp barcode) ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA was extracted from pure Kp cultures using the DNeasy PowerSoil Pro Kit (Qiagen, Hilden, Germany). Long-read genomic sequencing was performed using GridION X5 (Oxford ...
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bioRxiv - Plant Biology 2021Quote: ... DNA extractions were performed on mechanically lysed samples using the PowerPlant Pro DNA isolation kit (Qiagen, CA), following the protocol described by Solanki et al ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from the ground biomass using DNeasy PowerPlant Pro Kit (Qiagen Corp., Germantown, MD, USA) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... Cecum content was collected and bacterial DNA was extracted using the DNeasy PowerSoil Pro Kit (Qiagen, Germany). qPCR was performed using the FastStart Universal SYBR Green Master Mix (Roche ...
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bioRxiv - Genomics 2021Quote: ... using the CD1 lysis buffer and beating beads from a Qiagen PowerSoil Pro Kit (Qiagen, Hilden, Germany) and then either the remaining components of the PowerSoil Pro kit or DNA purification beads from Kapa Biosciences (Roche ...
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bioRxiv - Microbiology 2021Quote: ... Any sample that failed to properly amplify was cleaned using the DNeasy PowerClean Pro Cleanup Kit (Qiagen). PCR products were cleaned on a per-sample basis using AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Microbiology 2021Quote: ... DNA extractions were carried out on ground root and soil samples using DNeasy PowerPlant Pro kit (Qiagen) following the manufacturer’s instruction.
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bioRxiv - Physiology 2023Quote: Genomic DNA extraction from fecal samples was performed using the QIAamp PowerFecal Pro DNA Kit (Qiagen, Germany). The quantity and quality of extracted DNA were measured using Qubit 3.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from cloacal swab and stool samples using the Powersoil Pro DNA Extraction kit (Qiagen) according to manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... Microbial DNA was extracted using the DNeasy PowerSoil Pro Kit following the provider’s instructions (Qiagen, Hilden, Germany). DNA samples were sent to the NGS Competence Center Tübingen (Tübingen ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted using protocols from the DNeasy PowerSoil Pro Kit with some modifications (Qiagen, Hilden, Germany) as described previously4 ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted using DNAeasy Power Soil Pro kit or DNAeasy PowerMax Soil kit (Qiagen, Kista, Sweden) for 47 mm and 142 mm filters ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from 25 mg brain samples using the DNeasy PowerSoil Pro Kits (Qiagen, Germany, 47014) following the manufacturer’s protocol with the following modifications ...
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bioRxiv - Genomics 2022Quote: ... Quantity was confirmed of all controls with the Investigator® Quantiplex® Pro kit (QIAGEN, Hilden, Germany) on the ABI 7500 (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... All samples in this validation were quantified using the Investigator Quantiplex® Pro kit (QIAGEN, Hilden, Germany) on the 7500 Real-Time PCR System (Applied Biosystems™ ...
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bioRxiv - Microbiology 2022Quote: ... Bulk DNA was also extracted from stool samples (DNeasy PowerSoil Pro DNA isolation kit, Qiagen, Hilden, Germany) to serve as a template for PCR using primers targeting the bacterial 16S rRNA encoding gene ...
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Contribution of circulating host and microbial tryptophan metabolites towards Ah receptor activationbioRxiv - Biochemistry 2023Quote: ... Bulk DNA was also extracted from stool samples (DNeasy PowerSoil Pro DNA isolation kit, Qiagen, Hilden, Germany) to serve as a template for PCR using primers targeting the bacterial 16S rRNA encoding gene ...
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bioRxiv - Microbiology 2023Quote: ... The other aliquot was used for DNA extraction using the DNeasy Powersoil Pro Kit (QIAGEN, Hilden, Germany) and molecular detection of Phytophthora using the nad1 PCR as described in the “Molecular identification” section.
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bioRxiv - Microbiology 2024Quote: ... 16 Dec 2021 and 11 May 2022 samples was extracted with the DNeasy PowerSoil Pro Kit (Qiagen). The manufacturer’s instructions were followed ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was then extracted from each worm using the Powerfecal Pro extraction kit (Qiagen, Product number 51804), as per the standard operating protocol ...
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bioRxiv - Immunology 2024Quote: ... Genomic DNA was extracted from swabs using a PowerSoil Pro kit on a Qiacube HT (QIAGEN, MD). One-step PCR library prep and dual-index paired-end Illumina sequencing was used to sequence the skin microbiome of individual frogs ...
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bioRxiv - Genomics 2024Quote: DNA was extracted according to protocol using QIAGEN DNeasy PowerSoil Pro Kit (Cat# 47016, QIAGEN, Hilden, Germany) with mechanically lysed using QIAGEN PowerLyzer 24 Homogenizer (110/220 V ...
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bioRxiv - Microbiology 2024Quote: ... and stored at -80°C until DNA was extracted using the DNeasy PowerSoil Pro kit (47014, Qiagen). Tube two ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial DNA was extracted from the pellet using a QIAamp PowerFecal Pro DNA kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: The different methods tested were DNA_P1) QIAsymphony® PowerFecal® Pro DNA Kit (cat. no. 938036, Qiagen) including six rounds of homogenization on a FastPrep-24™ Classic at 6 m/s for 60 seconds ...
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bioRxiv - Microbiology 2024Quote: ... Extraction was performed on the 9 samples using the DNeasy® PowerSoil® Pro Kit (QIAGEN, Germany) according to the manufacturer’s protocol.
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bioRxiv - Genetics 2024Quote: ... and M-Vac filters using DNeasy® PowerSoil® Pro Kit (Catalog number 47016, QIAGEN, Hilden, Germany) with some minor modification to steps 1-4 of the manufacturer’s protocol (DNeasy PowerSoil Pro Kit Handbook 06/2023) ...
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bioRxiv - Immunology 2024Quote: Microbial DNA was obtained from fecal samples (humans or mice) using the DNeasy PowerSoil Pro kit (Qiagen) according to manufacturer’s instructions with an added cell lysis step at TissueLyser II bead beater (Qiagen) ...
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bioRxiv - Systems Biology 2024Quote: DNA was extracted from the fecal samples using the Power Soil Pro Kit protocol (Qiagen, Germantown, MD). Barcoded primers targeting the V4 region of the16S rRNA gene were used to PCR amplify the extracted bacterial DNA using the Earth Microbiome Project (EMP ...
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bioRxiv - Microbiology 2020Quote: ... BipA-His was purified from the soluble fraction by affinity chromatography using Ni-NTA agarose (QIAGEN) previously equilibrated with equilibration buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 6x-His-Smt3 was purified from BL21 E.coli cells using a Ni-NTA affinity column (Qiagen) following manufacturer indications ...
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bioRxiv - Cancer Biology 2021Quote: ... Signal was detected using mouse anti-His antibody from Qiagen (Cat No./ID: 34660 1:500). Anti-mouse HRP was used for colorimetric development (Cat No./ID-A8924 1:1000) ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged BASP1 derivatives were prepared using Ni-NTA beads following the manufacturers instructions (Qiagen). Purified proteins were dialysed into Buffer D and stored at −80°C.
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bioRxiv - Plant Biology 2021Quote: ... HIS-tagged proteins were purified by metal-affinity chromatography using Ni-NTA resins (Qiagen, Hilden, Germany). Protein concentration was estimated by the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and His-tagged Fabs were isolated from cell supernatants using Ni-NTA columns (Qiagen, Hilden, Germany). IgGs ...
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bioRxiv - Plant Biology 2020Quote: ... The expressed protein was purified as His-tagged fusion protein using Ni-NTA agarose beads (Qiagen) and analyzed by SDS-PAGE.
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bioRxiv - Neuroscience 2020Quote: ... The His-tagged target protein was purified by Ni-NTA gravity-flow chromatography (Qiagen, Hilden, Germany). The eluted protein was subjected to MonoQ 10/100 GL (GE Healthcare Lifesciences ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins modified with 6×-His-Ub was purified using Ni-NTA Sepharose (142350243, Qiagen, Alameda, CA) on a rotating platform for 2 hours at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified using the His-tag Protein Purification Kit (Ni-NTA Agarose, QIAGEN GmbH), GST-tag Protein Purification Kit (Glutathione Sepharose ...
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bioRxiv - Biophysics 2023Quote: ... the glass was additionally treated with biotinylated anti-hexahistidine monoclonal antibody (Penta-His Biotin Conjugate; Qiagen) as in Duchi et al.23 and Dulin et al.31.
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bioRxiv - Microbiology 2024Quote: ... sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting using Penta-His antibody (QIAGEN)) were pooled and concentrated using Amicon filter devices with 10 kDa molecular weight cut-off (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 × 106 MES or CP cells were harvested and RNA extraction was performed using Rneasy mini plus kit (Qiagen). 1 μg of total RNA was used for the construction of sequencing libraries and sequencing.
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bioRxiv - Microbiology 2022Quote: Total genomic DNA of mouse fecal samples was extracted using QIAamp PowerFecal Pro DNA Kit (Qiagen, CAT#: 51804), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... and DNA was extracted from 50 mg of material using the DNeasy PowerPlant Pro Kit (Qiagen, Hilden, Germany). Extracted DNA was taken to the Genomics Core at the University of Tennessee ...
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bioRxiv - Microbiology 2022Quote: ... the bacterial assemblage genomic DNA was extracted from one g of beads using DNeasy PowerSoil Pro kit (QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Genetic material was isolated from frozen cecum samples using the QIAamp PowerFecal Pro DNA Kit (Qiagen, Germantown, MD). Bead beating was performed for 3 min at 6.5 m/s on a FastPrep-24 system (MP Biomedicals ...
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bioRxiv - Microbiology 2022Quote: ... against five other extraction kits: the Qiagen MagAttract PowerSoil Pro DNA Isolation Kit (Cat#: 47109; Qiagen, Carlsbad, CA), the Norgen Stool DNA Isolation Kit (Cat# 65600 ...