Labshake search
Citations for Qiagen :
551 - 600 of 773 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... we generated a blank library by amplifying a control DNA template derived from a DNeasy PowerSoil Pro kit (Qiagen) extraction on molecular-grade water ...
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bioRxiv - Molecular Biology 2023Quote: ... rdr1;2;6 Cen5-ATHILA5 ddm1 (hp5) and their corresponding DDM1 wild-type siblings with the DNeasy Plant Pro Kit (Qiagen). From each sample ...
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bioRxiv - Physiology 2023Quote: DNA extraction was performed from 0.1 g of fecal sample using QIAamp PowerFecal Pro DNA Kit (Qiagen, Hilden, Germany). The solid fecal sample was added into PowerFecal Pro tubes containing pre-filled beads and the extraction was performed according to the manufacturer’s instruction with additional bead-beating step (ensruing a total of three bead-beating steps ...
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bioRxiv - Systems Biology 2023Quote: ... DNA was extracted from the fecal slurries with the DNeasy PowerSoil Pro Kit on a QiaCube HT (Qiagen, Germany) and quantified using the Qiant-iT Picogreen dsDNA Assay (Invitrogen ...
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bioRxiv - Genomics 2024Quote: DNA was extracted from fresh soil and plant root samples (0.25 g) using the DNeasy PowerSoil Pro Kit (QIAGEN) according to the manufacturer’s protocol and then sent to the company (Magigene ...
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bioRxiv - Microbiology 2024Quote: DNA from each soil sample was extracted from 250 mg of soil using the DNeasy PowerSoil Pro Kit (QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... genomic DNA was extracted from mycelial mats of three isolates using DNeasy Plant Pro Kit (Qiagen Inc., Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic bacterial DNA was extracted from frozen stool samples using the QIAamp PowerFecal Pro DNA Kit (Qiagen, Germantown, MD). Bead beating was implemented in three cycles for DNA extraction ...
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bioRxiv - Microbiology 2024Quote: ... and 150-175 cm depth increments were collected and extracted for DNA using the MoBio PowerSoil Pro Kit (QIAGEN) and resuspended in 50 μL of 10 mM Tris buffer ...
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bioRxiv - Microbiology 2024Quote: ... Moore swabs were cut into two pieces and processed using either the DNeasy PowerSoil Pro Kit (Qiagen cat # 47014) (Tube 1 ...
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bioRxiv - Genomics 2023Quote: ... and (d) Purify DNA using MinElute column (Qiagen) as directed by manufacturer ...
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bioRxiv - Biochemistry 2020Quote: ... DNA constructs were amplified in Escherichia coli DH5α and purified using a Hi-Speed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Biochemistry 2020Quote: ... (2010) using commercial antibodies directed against the His-tag following the instructions of the manufacture (Qiagen, Hilden, Germany).
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bioRxiv - Cell Biology 2021Quote: Antibodies and their conditions of use are as follows: mouse anti-His (Western blot, 1:1000; 34660; Qiagen), rabbit anti-Brl1 (Western blot ...
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bioRxiv - Microbiology 2020Quote: ... The 6 × his-tagged proteins were purified using a nickel-nitrilotriacetic acid (Ni-NTA) agarose matrix (30250, Qiagen). Cell-free extracts were loaded on 0.5 ml Ni-NTA matrixes and incubated on a roller shaker for 2 h at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... The His-tagged proteins were purified by Ni-affinity gravity-flow chromatography using the Ni-NTA agarose (Qiagen) following the product manual.
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bioRxiv - Molecular Biology 2020Quote: ... His-tagged ubiquitin-conjugated proteins were purified by adding 100 µL of 50 % Ni-NTA agarose beads (Qiagen) equilibrated in A2 buffer to 1 mL of cleared extracts and incubated with rotation for 2-4 hr at room temperature ...
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SARS-CoV-2 comprehensive receptor profiling: mechanistic insight to drive new therapeutic strategiesbioRxiv - Cell Biology 2021Quote: ... each pre-incubated at a 2:1 molar ratio with Penta His Alexa Fluor 647 Conjugate (Qiagen, UK). Hits (duplicate AF647 positive spots ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 RBD-His protein was purified from cell culture supernatant using a Ni-NTA (Qiagen) affinity column ...
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bioRxiv - Microbiology 2020Quote: A 6.5 nM concentration of His-tagged 16055 SOSIP.v8.3 trimer in TBS was added to a 96-well Ni-NTA plate (Qiagen) for 2 h at RT ...
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bioRxiv - Microbiology 2020Quote: ... His-SFP1 was purified using Ni-NTA affinity purification under native conditions using the standard manufacturer’s protocol (Qiagen). Recombinant His-SFP1 was used to immunize female New Zealand white rabbits (Covalab ...
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bioRxiv - Bioengineering 2020Quote: Initial small test purifications were performed using Ni-NTA Spin Column for His-Tagged proteins (Qiagen, Hilden, Germany) following the protocol for 6xHis-Tagged Proteins under Native Conditions from E ...
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bioRxiv - Biochemistry 2020Quote: ... The Ni-NTA agarose resin for purification of his-tagged proteins was purchased from Qiagen (Germantown, MD, USA). Superdex 75 16/600 and Superdex 200 Increase 10/300 size exclusion chromatography columns were purchased from GE Healthcare (Uppsala ...
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bioRxiv - Biochemistry 2021Quote: DNA constructs were amplified in Escherichia coli DH5α and purified using a Hi-Speed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Plant Biology 2024Quote: ... All fractions were analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting using anti-His (Qiagen) and anti-strep (MilliporeSigma ...
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bioRxiv - Molecular Biology 2024Quote: ... The agarose gel bound with the His-tagged recombinant protein was packed in the chromatography column (Qiagen, Germany), and the flow through was discarded ...
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bioRxiv - Microbiology 2023Quote: ... The N-terminal His-tagged Sif protein (Lmo0946-His6) was expressed and purified using Ni-NTA resin (Qiagen) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates of His-tagged talin constructs were purified by Ni-NTA-sepharose affinity chromatography (Ni2+-nitrilotriacetic acid, Qiagen), followed by a gel filtration column (Superdex 200 ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates of His-tagged vinculin constructs were purified by Ni-NTA-sepharose affinity chromatography (Ni2+-nitrilotriacetic acid, Qiagen), followed by a Q-Sepharose ion exchange column ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% CA630) and rotated at 4°C for 2 hours before addition of His-beads (Ni-NTA, QIAGEN). After continuous rotating for another 2 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... NusA–Strep tag–gene 60–6 × HIS proteins were purified by sequential chromatography on Ni-NTA agarose (Qiagen) and S-protein agarose (Novagen) ...
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bioRxiv - Immunology 2020Quote: ... B220+Fas+CD38- CD45.1+ or CD45.1-) were sorted from immunized recipients directly into RLT buffer supplemented with 1% β-ME (Qiagen, Hilden, Germany). mRNA was extracted with an RNeasy Micro Kit (Qiagen ...
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bioRxiv - Physiology 2020Quote: ... A cross-sectional piece of kidney containing cortex and medulla was homogenized in Buffer RLT with 0.01% β-ME and further homogenized with Qiashredder columns (Qiagen 79654). mRNA concentration was measured by Nanodrop (ThermoFisher) ...
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bioRxiv - Neuroscience 2024Quote: ... and microglia (treated for 6 hours) treated with or without 1 µM CDDO-Me with a RNeasy Mini Kit (Cat. number: 74106; QIAGEN, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... the DNA was extracted from 100 mg mouse fecal samples (9-week-old, 2 weeks after final oral gavage treatment) using QIAamp PowerFecal Pro DNA kit (Qiagen) following the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2020Quote: Around 250 mg of each produce sample was cut into 1-3 mm pieces using a scalpel and then processed with the DNeasy Powersoil Pro kit (Qiagen) to isolate 50μL of eluted DNA ...
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bioRxiv - Plant Biology 2021Quote: ... Genomic DNA was extracted from 600 mg fresh tissue per line using either the DNeasy Plant Pro kit (#69204, Qiagen) (Cam-1 and Cam-2 lines ...
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bioRxiv - Microbiology 2021Quote: ... bacterial DNA was extracted from pancreatic juice or duodenal juice samples using a DNeasy PowerSoil Pro kit (Qiagen, Venlo, Netherlands). Each library was prepared according to the Illumina 16S Metagenomic Sequencing Library Preparation Guide with the following primer sets targeting the V1–V2 region of 16S rRNA genes:27Fmod ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from an overnight culture of the mutant bacterial strain along with the WT strain using DNeasy PowerSoil Pro kit (QIAGEN), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Microbial DNA was isolated from 200 μL of homogenized fecal material using the DNeasy PowerSoil Pro extraction kit (Qiagen, Germany) with bead beating in Qiagen Powerbead Pro plates (Cat No ...
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bioRxiv - Microbiology 2022Quote: ... was extracted from isolated adult bee guts or whole-body for larva using a QIAamp PowerFecal Pro DNA Kit (Qiagen). Gut tract from each surface-sterilized adult bee was dissected in sterilized phosphate buffered solution (PBS ...
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bioRxiv - Microbiology 2022Quote: ... Standard curves were generated using known amounts of purified bacterial genomic DNA (gDNA) extracted from stable phase cultures using the DNeasy PowerSoil Pro Kit (Qiagen). Purified gDNA was quantified via nanodrop and used as stock for a 10-fold serial dilution standard curve ...
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bioRxiv - Microbiology 2022Quote: Total DNA was extracted from bulk (n = 23) and rhizosphere (n = 23) soils using the PowerSoil DNA Kit or the PowerSoil Pro Kit (QIAGEN) following the manufacturer’s protocols with minor modifications ...
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bioRxiv - Plant Biology 2022Quote: DNA was isolated from the lyophilized rhizosphere fraction and root powder using the DNeasy Powersoil Pro kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic bacterial DNA was extracted from these frozen stool samples utilizing the QIAamp PowerFecal Pro DNA Kit (Qiagen, Germantown, MD). To facilitate DNA extraction ...
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bioRxiv - Microbiology 2023Quote: Total DNA was extracted from frozen filtered membrane or sediment samples using the DNeasy PowerSoil Pro Kit (Qiagen, Hilden, Germany), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Total genomic DNA was extracted from the sampled material using the Dneasy® PowerSoil® Pro Kit (Qiagen, The Netherlands) according to the manufacturer’s instructions with noted alterations ...
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bioRxiv - Microbiology 2023Quote: ... DNA for metagenomes was extracted from 5 and 0.2 μm filters (47 mm diameter) using the DNAeasy Power Soil Pro kit (Qiagen, Sweden). DNA from viral fraction (142 mm diameter membranes ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from biomass samples taken at the end of the anoxic period after 68 days of operation using the DNeasy PowerSoil Pro Kit (Qiagen), according to the manufacturer’s instructions with the following exceptions ...
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bioRxiv - Microbiology 2023Quote: ... The Duke Microbiome Shared Resource (MSR) extracted DNA from stool samples using a Qiagen DNeasy PowerSoil Pro Kit (Qiagen, 47014). Bacterial community composition in isolated DNA samples was characterized by amplification of the V4 variable region of the 16S rRNA gene by polymerase chain reaction using the forward primer 515 and reverse primer 806 following the Earth Microbiome Project protocol (http://www.earthmicrobiome.org/) ...