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Citations for Qiagen :
601 - 634 of 634 citations for Octreotide acetate impurity C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Data to derive different biological clocks was available for different subsamples and all based on a fasting blood draw from participants in the morning between 8:30 and 9:30 after which samples were stored in a −80°C freezer or – for RNA - transferred into PAXgene tubes (Qiagen, Valencia, California, USA) and stored at −20°C ...
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bioRxiv - Cell Biology 2022Quote: ... The reaction mixture was incubated at 37°C for 24 hours and subsequently underwent RNA clean-up using a column-based purification (Qiagen, product number 74104). The purified RNA was eluted in nuclease-free water (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... Efficient electroporation was confirmed by observation of fluorescence before genomic DNA extraction around 16 hpf (18°C) using QIAamp DNA Micro kit (Qiagen, German Town, MD). Mutagenesis efficacy of individual sgRNAs ...
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bioRxiv - Neuroscience 2021Quote: ... The mixture was incubated at 37 °C for various times and the DNA was purified using a MinElute PCR purification kit (Qiagen, Germantown, MD, USA). Then ...
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bioRxiv - Molecular Biology 2022Quote: ... and the DNA pellet was stored at −80°C until purification was performed using a commercial kit (DNeasy Blood and Tissue Kit, catalog #69504; Qiagen; Germantown, MD, USA) according to manufacturer’s instructions with a minor modification to the elution step ...
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bioRxiv - Microbiology 2022Quote: ... strain SPR117 was grown during 7 days in R2A broth at 20 °C and its genomic DNA (gDNA) was extracted using the Dneasy UltraClean Microbial Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Brain parts were stored in 100 µL of Trizol™ in -80 °C for later RNA extraction using RNAeasy Mini Extraction Kit™ (Qiagen, Germany) according to the manufacturers protocol ...
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bioRxiv - Genomics 2021Quote: ... individuals were flash frozen in liquid nitrogen and stored in −80°C until DNA extraction using the Qiagen DNeasy Plant kit (Qiagen, Valencia, CA, USA) as described in (Kainer et al ...
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bioRxiv - Biochemistry 2021Quote: ... Medium was stirred for 1 hour at 4°C in the presence of 5 ml Ni-NTA Agarose beads (Qiagen, Venlo, The Netherlands) washed with 25 mM Hepes-KOH pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The soluble fraction was separated from debris by centrifugation (9000 g, 4°C) and incubated for 60 minutes with Ni-NTA beads (Qiagen, cat. no. 30230) pre-equilibrated in binding buffer ...
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bioRxiv - Microbiology 2023Quote: ... approximately 1 x 104 colonies were collected and pooled into LB-amp culture media and then incubated at 37°C for five hours prior to harvesting the bacterial population for plasmid purification by Maxiprep (Qiagen Plasmid Maxi Kit). The presence of a diverse barcode in the plasmid stock was verified by next generation sequencing (see below) ...
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bioRxiv - Molecular Biology 2024Quote: Total bacterial DNA was extracted from frozen (−80°C) fecal samples from AuPhos- or vehicle treated mice using PowerFecal DNA/RNA Kit (Qiagen, Hilden, NRW, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: Isolated human LGR5+ cells were stored at −80°C until processed for RNA isolation using RNeasy micro kit as per manufacturer instruction (Qiagen, Germantown, MD, USA). RNA processing ...
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bioRxiv - Cancer Biology 2023Quote: Cells were harvested (1×106) by centrifugation (200 x g for 5 min at 25°C. Whole RNA was isolated using the Qiagen RNeasy kit (Qiagen) according to the manufacturer’s description ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellets were kept at −80°C until performing the DNA extraction using DNAeasy Blood & Tissue Kit (QIAGEN; Cat. No. ID: 69504). Qubit Broad Range double-stranded DNA assay (Life Technologies ...
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bioRxiv - Biophysics 2024Quote: ... for 30 min at 4 °C before being applied to a 10mL bed volume Nickel nitrilotriacetic acid (Ni-NTA) agarose (Qiagen, cat. no. 30230) column ...
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bioRxiv - Molecular Biology 2024Quote: ... Liquid cultures were grown at 37°C overnight to saturation and DNA was extracted using QIAGEN® Plasmid Plus Midi Kit (Qiagen cat#12945)
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bioRxiv - Developmental Biology 2024Quote: ... Human islets and stem cells were pelleted and stored at -80°C until DNA extraction using the Blood/Tissue DNeasy kit (Qiagen; Germantown, MD, USA) as per manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... then stored at -80°C until RNA isolation using the Qiagen RNeasy Mini kit according to the manufacturer’s recommendations (QIAGEN Sciences, Germantown, MD, USA). Each RNA sample represented the total RNA isolated from two pooled antennae of each individual ...
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bioRxiv - Molecular Biology 2020Quote: ... Animals were raised at 25°C for 48 h after hatching and total RNA was purified by using RNeasy® Mini Kit (Qiagen, Venlo, The Netherlands). Microarray and the following data analysis were performed as described before (Qi et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Enzyme was inactivated at 95 ° C for 10 min and 700 μl Trizol was added followed immediately by RNA extraction using the miRNeasy kit (Qiagen Inc., Valencia, CA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... Both the input and IP samples were incubated at 50°C for 2 hr and then cleaned up using the Qiaquick PCR Purification Kit (Qiagen, Hilden, Germany, Cat # 28104) and eluted in 35 μL of water.
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bioRxiv - Molecular Biology 2022Quote: Total RNA was prepared from synchronized day one adult animals raised at 20°C by using RNeasy® Mini Kit (Qiagen, Venlo, The Netherlands). Oligo-dT primer was used to synthesize cDNA by using Transcription First Strand cDNA synthesis kit (Roche ...
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bioRxiv - Genetics 2024Quote: The organs of Corti of postnatal day (P)4 mice were dissected and stored at -20°C in RNAprotect Tissue Reagent (RNAlater®) (QIAGEN, cat. no. 76106). Total RNA was extracted using the SPLIT RNA extraction kit (Lexogen ...
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bioRxiv - Plant Biology 2023Quote: ... All soil samples were stored at –20°C until rhizosphere DNA was extracted using a QIAGEN DNeasy PowerSoil Kit (Qiagen, Inc., Valencia, CA, USA) following the manufacturer’s protocol.
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bioRxiv - Systems Biology 2020Quote: ... was prepared by centrifuging a cell culture at 5525xg for 2 minutes at 4°C then resuspending cells in 1mL of PBS-RNAprotect (333 μL RNAprotect Bacteria Reagent [76506, Qiagen, Hilden, Germany], 666 μL PBS). For immediate RNA stabilization by RNAprotect (Fig ...
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bioRxiv - Cell Biology 2020Quote: ... The relative expression of mRNAs of c-Myc regulated genes identified in RT2 Profiler™ PCR Array Human MYC Targets (Qiagen, Cat. no. PAHS-177Z) was determined by real-time quantitative RT-PCR ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed twice with the blocking buffer and incubated at 4 °C with primary antibodies (mouse anti-Strep, Qiagen, Cat #: 34850, 1: 150 dilution) (rabbit anti-PDI ...
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bioRxiv - Genomics 2022Quote: An aliquot of 150 μL of plasma per sample was thawed on ice and centrifuged at 3000 × g for 5 min at 4 °C and smallRNA was extracted using miRNeasy Serum/Plasma Kits (Qiagen, Cat. No. 217184, Milano, Italy) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was extracted from homogenates after overnight incubation with 20 μl of proteinase K at 56°C using the QIAamp DNA Micro Kit (Qiagen, Hilden, Germany, cat. No. 56304) following the manufacturer’s protocol for tissue samples ...
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bioRxiv - Cell Biology 2023Quote: ... RNA and protein were digested in 40 mM Tris-HCl pH 6 and 10 mM EDTA by adding first RNAse A (Qiagen; #19101, 1.5 hours, 37°C), followed by Proteinase K (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... The remaining cells were pelleted by centrifugation (4000rpm, 30min, 4C) and stored at –20°C for bulk EEV extraction by endotoxin-free maxiprep (Qiagen EndoFree Maxi Kit, Cat. 12362).
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bioRxiv - Molecular Biology 2024Quote: ... DNA pellets were washed 3X with 70% ethanol and hydrated in pre-warmed (37°C) Elution buffer (Qiagen, 10 mM Tris-Cl, pH 8.5. Cat. 19086) and incubated on HulaMixer™ Sample Mixer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... 5’ and 3’ untranslated regions and open reading frames) for the paralogs were made using the CLC Main workbench 7 (QIAGEN® Aarhus A/S, Aarhus C, Denmark). Sequence alignments were generated for the genomic DNA ...