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Citations for Qiagen :
201 - 250 of 634 citations for Octreotide acetate impurity C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... at -20°C or immediately processed for RNA using the RNeasy Mini Kit as per manufacturer’s protocol (Qiagen). Real-time analyses were performed as previously described (Van Otterloo et al. ...
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bioRxiv - Biochemistry 2022Quote: ... 4 °C for 1 h and purified by affinity chromatography using a 5 ml Ni-HP column (Qiagen). Flow through with pure AstaPo1 was collected and dialyzed overnight against the buffer containing 50 mM Tris pH 7.6 ...
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bioRxiv - Genomics 2023Quote: ... or the buffy coat of the BALB/c strain using the QIAsymphony DNA Mini Kit (cat:931236, Qiagen). The extracted gDNA was sheared to 150 bp in size using a Covaris LE 220 instrument ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% CA630) and rotated at 4°C for 2 hours before addition of His-beads (Ni-NTA, QIAGEN). After continuous rotating for another 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... 10 minutes 4°C and the supernatant was incubated with pre-equilibrated Ni-NTA agarose beads (Qiagen, #1018244) with shaking on ice for 3 hours ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA was stored at −80°C until further clean-up using RNeasy Mini kit (Qiagen, catalog #74104) manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... One hour of tagmentation at 37°C was followed by DNA extraction using MinElute PCR Purification Kit (Qiagen). Extracted DNA was subjected to PCR amplification using unique primers sets (Nextera XT v2 Full set (N7-S5)) ...
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bioRxiv - Biochemistry 2023Quote: ... The mixture was incubated at 37 °C for 15 min and then quenched by adding RLT buffer (Qiagen). The RNA was then extracted using RNeasy kit (Qiagen) ...
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bioRxiv - Immunology 2024Quote: ... Genomic DNA from the Swedish cohort was extracted from whole blood using Puregene Blood Core Kit C (Qiagen). Illumina whole-genome sequencing ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria were centrifuged at 4°C and washed with pre-cold PBS containing RNA stabilization solution (RNAprotect, Qiagen) before flash freezing of the pellets and storage at -80°C ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted from –80 °C-stored microcosm recovered cell samples using the DNeasy PowerSoil Pro kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... the homogenates were incubated overnight at 56°C with 20 µL of 20 mg/mL protease K (Qiagen). The crude extract was purified using the QIAamp DNA Mini Kit (Qiagen ...
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bioRxiv - Plant Biology 2024Quote: ... The samples were then placed at −80°C until grinding by a tissue-lyser (TissueLyser II, Qiagen, Germany).
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bioRxiv - Biophysics 2024Quote: ... for 4 hours at 37°C and purified from a 2% agarose gel (QIAquick Gel Extraction Kit, Qiagen). At this stage ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was the incubated at 4 °C with 2 ml of washed Ni-NTA agarose beads (Qiagen) for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were incubated on a Thermomixer (37°C, 30 min, 500 rpm) before being subjected to MinElute (Qiagen) cleanup to extract tagmented DNA ...
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bioRxiv - Biochemistry 2024Quote: ... for 45 min at 4°C and the supernatant was mixed with 3 mL Ni-NTA agarose (Qiagen) pre-equilibrated in lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant both N-and C-terminally His-tagged proteins were purified using a Ni-NTA Superflow cartridge (1ml, Qiagen) and the Fast Protein Liquid Chromatography (FPLC ...
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bioRxiv - Immunology 2021Quote: The indicated cell types were harvested and stored at −80°C until RNA was isolated using RNeasy kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... supplemented with 100 μg/mL of carbenicillin for 16 hr at 30°C followed by plasmid purification (Qiagen, 12991).
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bioRxiv - Biochemistry 2020Quote: ... Lysates were centrifuged at 30,597xg and 4°C for 20min and the supernatant was bound to Ni-NTA Agarose resin (Qiagen), washed with 10 CV of wash buffer (50 mM HEPES-KOH pH=7.5 ...
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bioRxiv - Cancer Biology 2020Quote: DNA isolation was performed by adding proteinase K and incubation at 56°C using QIAamp DNA Mini Kit (Qiagen). DNA was quantified using a Qubit Fluorometer (ThermoFisher) ...
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bioRxiv - Genetics 2020Quote: ... stored at −80°C) and PBMCs were extracted using QIAGEN DNeasy Blood and Tissue kit (Qiagen Sciences, Maryland, USA), following manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared using a JA-20 rotor (18,000 rpm for 30 min at 4°C) and incubated with Ni-NTA superflow resin (Qiagen) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: Tissue lysates were further homogenized and centrifuged at 10,000 g for 10 min at 4°C in microcentrifuge spin columns (QIAshredder, Qiagen) to obtain clear protein lysate ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was isolated from a bank of frozen brains kept at −80 °C using a RNA isolation kit according to the manufacturer’s instructions (Qiagen). Frozen brain samples were thawed and dissected from 5 different regions ...
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bioRxiv - Plant Biology 2021Quote: ... the protein was removed by 1.1 μL of proteinase K (20mg/ml) at 45° C for 1h and RNA by 2μL of RNaseA (1mg/ml) (Qiagen) digestion at 37°C for 1h ...
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bioRxiv - Microbiology 2021Quote: ... Membrane filters were stored at −80°C until DNA could be extracted using a DNeasy Power Water kit (Qiagen) and bacteria were quantified using qPCR of the vvhA gene.
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bioRxiv - Plant Biology 2020Quote: ... 10mM imidazole was added and incubated on rotator for overnight at 4°C in the presence of 2.5ml of Ni-NTA beads (Qiagen). The beads were washed with EB containing 6M guanidine-HCl and 0.25% Triton X-100 ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant obtained by 5 minutes of centrifugation at 16.000 g of bacterial lysate was stored at −80 °C or immediately purified with the miRNeasy mini kit (Qiagen). RNA quantity was determined using the Qubit 4 Fluorometer and Nanodrop spectrophotometer (Thermofisher) ...
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bioRxiv - Microbiology 2020Quote: ... Crystals of Brd4(BD1) with YFV-C (1-10) K4ac peptide were grown from a solution containing 0.2 M sodium chloride (QIAGEN), 0.1 M Tris (pH 7.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting in buffer A supplemented with 200 mM imidazole in gravity flow ...
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bioRxiv - Molecular Biology 2022Quote: ... counted and PBS washed pellets were stored at -80 °C for subsequent gDNA extraction (QIAamp DNA Mini, QIAGEN 51304). DNA content was determined with Qubit dsDNA HS Kit (Thermo Fisher Q32854 ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting with buffer A supplemented with 250 mM imidazole in gravity flow ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 mM GDPNP-Mg2+) were obtained at 4°C using 0.2 M Lithium nitrate and 20% PEG3350 (PEG Suite I, Qiagen) as precipitating agent ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was subjected to Ni-NTA resin (QIAGEN), washed and protein eluted with buffer B supplemented with 250 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... cleared by centrifugation (20 min, 20,000 × g, 4°C) and the clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting in buffer C supplemented with 200 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA agarose resin (QIAGEN) eluted with buffer A supplemented with 0.5 mM TCEP and 200 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and cleared lysate was subjected to Ni-NTA resin (QIAGEN), washed and protein eluted with buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Systems Biology 2022Quote: ... Ten millilitres of culture were pelleted by immediate centrifugation (5,000 × g for 3 min at 4°C) and resuspended in 5 mL of RNAlater (76106; Qiagen). Samples were stored at 4°C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... The lysates were then incubated at 95°C for 5 min and filtered using a QIAshredder homogenizer (Qiagen, 79656). The extracted proteins were analyzed using 5–20% gradient sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE ...
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bioRxiv - Microbiology 2022Quote: ... and stored pellets at −80 °C for <1 week before RNA extraction using RNeasy Protect Bacteria Mini Kit (Qiagen) according to the manufacturer’s instructions (protocol #5) ...
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bioRxiv - Immunology 2020Quote: ... ConM-SOSIP.v7 carrying a C-terminal His-tag (diluted to 6.5nM in TBS) was immobilized onto 96-well Ni-NTA ELISA plates (Qiagen) by incubation for 2 hours at room temperature after which the plates were washed 3 times with TBS ...
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bioRxiv - Biophysics 2020Quote: ... Debris was removed by centrifugation (20,000×g, 30 min, 4°C) and the supernatant was incubated with Ni-NTA resin (Qiagen) for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... was tagmented with Tn5 transposase (25 μM) for 30 min at 55°C and purified using MinElute columns (QIAGEN).
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 30 min at 45,000 × g at 4°C and incubated with 1 ml of Ni-NTA agarose beads (Qiagen) per 1 l of expression culture for 2 h rotating at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were clarified by centrifugation for 1 h at 4°C at 10,000 rcf before being subjected to Ni-NTA (Qiagen) affinity purification following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2021Quote: ... The solution was further cleared at 20,000 × g at 4 °C and the supernatant was incubated with Ni-NTA resin (Qiagen) for 2 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were expressed in BL21de3 E.coli by auto induction in LB medium at 18°C and purified using Ni-NTA beads (Qiagen). When present ...
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bioRxiv - Microbiology 2020Quote: ... at 37°C and genomic DNA was extracted using the Qiagen DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA). The concentration of resultant DNA was determined using a Qubit double-stranded DNA BR assay kit and a Qubit fluorometer (ThermoFisher Scientific ...