Labshake search
Citations for Qiagen :
551 - 600 of 634 citations for Octreotide acetate impurity C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... The dried DNA pellet was reconstituted in 50 μL H2O treated with 330 μg/mL of RNase A for 2 hours at 37°C and then recovered using the QIAquick PCR Purification kit (QIAGEN Cat#28104). DNA concentration was determined by Qubit (Thermofisher).
-
bioRxiv - Plant Biology 2020Quote: ... Plant material was dried at 60°C overnight and ground to powder using a ball mill (Tissue Lyser II, Qiagen, Venlo, Netherlands). Aliquots of about 1.5-2 mg were transferred into tin capsules (IVA Analysentechnik ...
-
bioRxiv - Plant Biology 2022Quote: ... Tissue was ground to a fine powder at −80°C using 3-mm stainless steel beads at 50 Hz for 2*30 seconds in a TissueLyser LT (Qiagen, Germantown, USA). Ground samples were extracted with 1 mL of cold methanol containing [phenyl 13C6]-IAA (0.1 nmol/mL ...
-
bioRxiv - Microbiology 2022Quote: ... aeruginosa cells were lysed after 18 hours using 2.5mg/mL lysozyme and incubated at 37°C for 30 minutes and RNA was extracted using the RNeasy mini kit (QIAGEN, Germantown, MD). DNase treatment was done twice for better DNA removal using Qiagen RNase-free DNase during RNA extraction and using NEB DNase after RNA extraction ...
-
bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from 12-d-old seedlings grown at 22°C in LD conditions at ZT13 with the RNeasy® Plant Mini Kit (QIAGEN, Germany) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... spun down (400 g × 10’ at 4 °C) and the pellet was resuspended in 600μ of Qiazol Lysis Reagent (Qiagen, Cat.No. 79306) and kept at −80 °C before sequencing.
-
bioRxiv - Microbiology 2020Quote: ... bearing a C-terminal histidine tag (ACRO Biosystems, Newark, NJ) was coated at 2 μg/ml on a Ni-NTA plate (Qiagen, Valencia, CA). After washing and blocking ...
-
bioRxiv - Developmental Biology 2022Quote: ... was linearized with BbsI-HF restriction enzyme for 1 hour at 37°C and gel purified with QIAquick® Gel Extraction Kit (Qiagen, 28704). Two gRNAs targeting ITGBβ1 (Horlbeck et al. ...
-
The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: Cells were lysed in RLT PLUS buffer and stored at -80°C until RNA isolation with the RNeasy Micro Kit (Qiagen, Hilden, Germany). cDNA was generated using the iScript Advanced cDNA generation kit (Bio-Rad ...
-
bioRxiv - Biochemistry 2020Quote: ... at 4°C and the supernatant was loaded onto a 1 ml nickel nitrilotriacetic acid-agarose column (Ni-NTA, Qiagen, Hilden, Germany) previously equilibrated with the corresponding lysis buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... step for 15 min at 4°C to remove insolubilized material and the supernatant was incubated with Ni-NTA agarose beads (Qiagen, cat: 30230) for 2 hrs ...
-
bioRxiv - Biochemistry 2020Quote: Total RNAs were extracted from Wildtype MIN6 and MIN6 stably expressing shRNAs (non-specific/NS, C, D) using RNeasy kit (QIAGEN, Germantown, MD) and cDNAs were prepared using qScript cDNA SuperMix (Quantabio ...
-
bioRxiv - Evolutionary Biology 2021Quote: Whole bodies of individual male flies were removed from the -80°C freezer and immediately homogenized in Qiazol lysis reagent (Qiagen, Hilden, Germany). The homogenate was treated with DNase I followed by addition of chloroform ...
-
bioRxiv - Microbiology 2021Quote: ... Triton X-100 1.2% and lysozyme 20mg/ml) and incubated at 37°C for 1 h and DNA was extracted with the DNeasy blood&Tissue kit (Qiagen, Courtaboeuf, France) following the manufacturer’s instructions ...
-
bioRxiv - Zoology 2021Quote: ... dead mites were pierced with an 18-gauge needle under a dissecting microscope and digested overnight in lysis buffer and proteinase K at 56 °C (Qiagen, Valencia, CA). Final DNA product from each mite was eluted in 60 µL of AE buffer ...
-
bioRxiv - Biophysics 2020Quote: ... The lysate was cleared by centrifugation at 18000 rpm at 4°C and the supernatant was loaded onto 5 mL Ni–NTA resin (Qiagen, Milan, Italy) equilibrated with binding buffer ...
-
bioRxiv - Microbiology 2021Quote: ... at 37 °C for 1 h and recovered by using RNeasy MinElute Cleanup kit as recommended by the manufacturer (Qiagen, Hilden, Germany). RNA yields were estimated by employing a Qubit® Fluorometer as recommended by the manufacturer (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2021Quote: ... Samples were centrifuged at 14,000 g at 4°C for 10 mins and the supernatant collected for processing using a RNeasy mini-isolation kit (Qiagen Ltd, UK) according to the manufacturers’ protocol ...
-
bioRxiv - Immunology 2021Quote: ... cells were spun down and the supernatant was removed to either store the cells at -80° C before proceeding with the analyses either as a pellet or to resuspend in RLT (Qiagen, cat 79216) + 1% 2-mercaptoethanol ...
-
bioRxiv - Immunology 2020Quote: Fecal pellets were placed in a sterile round-bottom tube and stored at −20 °C until processed for DNA extraction using the QIAamp DNA Stool Mini Kit (Qiagen, Germantown, MD). DNA concentrations determined spectrophotometrically (Molecular Devices ...
-
bioRxiv - Cell Biology 2020Quote: ... coli cells were cultured at 37°C and plasmid isolation was carried out using a Qiagen Plasmid Mini Kit (Qiagen, Germantown, MD) according to manufacturer instructions ...
-
bioRxiv - Genomics 2021Quote: ... All collected plant tissues were frozen immediately in liquid N2 and stored at −70°C until the RNA was extracted and isolated using the RNeasy mini kit (Qiagen; Hilden, Germany) and library prepped using the SQK-PCS109 kit (Oxford Nanopore Technologies ...
-
bioRxiv - Developmental Biology 2022Quote: ... The PCR strips were then incubated for 15 min at 37 °C in a thermocycler and the reaction was stopped by adding 5 μl RLT plus buffer (Qiagen, Cat# 1053393) to each well ...
-
bioRxiv - Plant Biology 2022Quote: ... Tissue was ground to a fine powder at −80°C using 3-mm stainless steel beads at 50 Hz for 2*30 seconds in a TissueLyser LT (Qiagen, Germantown, USA). Ground samples were extracted with 1 mL of cold methanol containing [phenyl 13C6]-IAA (0.1 nmol/mL ...
-
bioRxiv - Neuroscience 2023Quote: ... Colonies were selected and grown in liquid broth (Fisher, catalog #12780052) overnight at 37 °C for DNA isolation (by QIAprep Spin Miniprep Kit, Qiagen, catalog #27106) and sequence verification (EuroFins Genomics).
-
bioRxiv - Pharmacology and Toxicology 2022Quote: Total mRNA was extracted from lung tissue kept in RNAlater stored at -20 °C using a RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2022Quote: ... Tissues were weighed and homogenized in 4 volumes of water (4 µL of water/mg tissue, 4°C) using a bead beater (TissueLyser II, QIAGEN; Germantown, MD). Aqueous homogenates were profiled using four complimentary liquid chromatography tandem mass spectrometry (LC-MS ...
-
bioRxiv - Microbiology 2023Quote: ... we dried both washed lichen fragments and wash-water pellets at 65°C and extracted DNA with the DNeasy Plant Mini Kit (QIAGEN, Hilden, Germany).
-
bioRxiv - Microbiology 2024Quote: ... The pellets were resuspended with OMV buffer and re-pelleted again by centrifugation at 100,000 g (31,000 RPM) for 2 h at 4°C and lysed with Qiazol followed by RNA isolation with the miRNeasy kit (Qiagen, Germantown, MD) to obtain total RNA including the small RNA fraction ...
-
bioRxiv - Cancer Biology 2024Quote: ... Targeted knockdown was conducted with 100nM ON-TARGETplus smartpool human c-MYC (mixture of 4 individual siRNA) and/or human KLF6-SV1 siRNA (Dharmacon, Lafayette, CO) with HiPerfect (Qiagen, Valencia, CA).
-
bioRxiv - Bioengineering 2024Quote: Whole mouse aortas (n=18 per group) cleaned of perivascular tissue were perfused with sterile HBSS and stabilized overnight at 4◦C in RNAProtect reagent (Qiagen; Hilden, Germany) before long-term storage at -80◦C ...
-
bioRxiv - Cancer Biology 2024Quote: ... and AR DBD domain DNA fragment were cut by NheI (N-terminal) and XhoI (C-terminal) and purified with the QIAquick Gel Extraction Kit (Qiagen; cat. 28704). After ligation with T4 DNA Ligase (New England ...
-
bioRxiv - Microbiology 2023Quote: DNA extraction from 0.25 g rhizosphere soil (frozen at −80°C) was performed using the DNeasy PowerSoil Pro kit (QIAGEN GmbH, Hilden, Germany) according to manual ...
-
bioRxiv - Developmental Biology 2022Quote: ... Tubes were placed at 37°C for 30 min and the resulting fragmented DNA was purified using the Minelute Cleanup Kit (Qiagen, Hilden, Germany), with elution performed in 10 µl of the provided elution buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... collected into an Eppendorf tube and centrifuged at 11200 rpm at 4 °C for 6 min followed by pellet resuspension with 200 μl of QIAzol (Qiagen, Hilden, Germany). To isolate EC from the bottom layer ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The dissected tissues were immediately placed on dry ice and stored at −80°C until RNA extraction was performed using the RNeasy Plus Micro Kit (Qiagen Catalog # 74034). Each fin bud was first homogenized in 50 μL of Buffer RLT (supplemented with β-mercaptoethanol per kit recommendation ...
-
bioRxiv - Genomics 2022Quote: ... we extracted total RNA from eight snap-frozen tissues kept at -80°C (brain, breast muscle, heart, kidney, liver, lung, spleen, and testis) using the RNeasy Mini Kit (Qiagen; Hombrechtikon, Switzerland) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... DNA was treated with proteinase K at 42°C for 2 hrs and purified using MinElute PCR Purification Kit (Qiagen, Cat# 28004).
-
bioRxiv - Cancer Biology 2023Quote: ... 45 cycles of denaturation at 95 °C for 15 s and annealing at 60 °C for 1 min following a melting step in a Rotor-Gene Q machine (Qiagen, Hilden, Germany). GAPDH was used for normalization ...
-
bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation at 20,000 x g for 45 minutes at 4°C and incubated with Ni-NTA agarose resin (Qiagen, catalogue number: 30230) pre-equilibrated with lysis buffer for 2 hours at 4°C ...
-
bioRxiv - Plant Biology 2023Quote: ... Collected tissue was ground to a fine powder at -80°C using 3 mm stainless steel beads at 50 Hz for 2*30 seconds in a TissueLyser LT (Qiagen, Germantown, USA), and between 10-15 mg of ground tissue per sample was used for auxin extraction ...
-
bioRxiv - Microbiology 2024Quote: ... then incubated at 37°C for five hours before harvesting the bacterial population for plasmid purification by Maxiprep (Qiagen Plasmid Maxi Kit). The presence of a diverse barcode in the plasmid stock was verified by next-generation sequencing (Figure S1) ...
-
bioRxiv - Genomics 2024Quote: ... we utilized the Integrative Genomics Viewer (IGV) v2.16.1[38] and the CLC workbench 7 (QIAGEN® Aarhus A/S, Aarhus C, Denmark). Furthermore ...
-
bioRxiv - Genomics 2024Quote: ... Cell debris was pelleted at 16,000g for 5 min and the supernatant was treated with 12µL of Proteinase K at 56°C for 15 min prior to purification with EZ1 DNA Tissue Kit (Qiagen; Cat. No. 953034) using the EZ1 Advanced XL machine (Qiagen) ...
-
bioRxiv - Genomics 2021Quote: Genomic DNA from MCF7 cells was isolated by proteinase K digestion at 65°C for 30 minutes followed by purification using the QIAamp circulating nucleic acid kit (Qiagen, Venlo, The Netherlands). Genomic DNA from frozen tissue sections of colorectal liver metastases was isolated using the NucleoSpin Tissue kit according to the manufacturer’s guidelines ...
-
bioRxiv - Molecular Biology 2020Quote: ... was digested overnight at 56°C in 20 ul proteinase K and 180 ul Buffer ATL from the Qiagen DNeasy Blood & Tissue Kit (Qiagen, Valencia, California, USA). DNA was extracted from the digest with the QIAamp PowerFecal DNA Kit (Qiagen ...
-
bioRxiv - Genetics 2020Quote: ... we grew one aliquot overnight in 400 mL LB + carbenicillin medium at 30°C and performed three maxi preps and two mega preps (200 mL each) using Qiagen Plasmid Plus Purification kits (Qiagen #12963, #12965, #12981). For the Upstream library ...
-
bioRxiv - Cell Biology 2021Quote: ... This was incubated in a 37 °C shaker for 1 hour before the addition of Proteinase K (10 µl, QIAGEN, >600 mAU / ml) and left at 55 °C for 1 hour ...
-
bioRxiv - Microbiology 2021Quote: ... Filters were flash-frozen in liquid nitrogen and stored at (−80°C) until extraction of DNA using the DNeasy Blood and Tissue Kit (cat. 69504 QIAGEN, Germantown, MD, USA) combined with QIAshredder (cat ...
-
bioRxiv - Microbiology 2021Quote: ... Buffer G2 and proteinase K were incubated at 56°C overnight and extracted using the EZ1 DNeasy Blood Tissue Kit (Qiagen GmbH, Hilden, Germany) in line with the manufacturer’s protocol.