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Citations for Qiagen :
551 - 600 of 10000+ citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... First strand cDNA was synthesized from 1 μg total RNA using the QuantiTect Reverse Transcription Kit (Qiagen). RT-qPCR was performed in a LightCycler480 (Roche ...
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bioRxiv - Plant Biology 2023Quote: ... or 500 ng of total RNA for reverse transcription using the One Step RT PCR-Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: cDNA was generated from 1 µg RNA with the Omniscript® Reverse Transcription Kit (Qiagen, Hilde, Germany) using oligoDT12-18 primer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Immunology 2022Quote: ... Removal of genomic DNA and subsequent complementary DNA was made using QuantiTect Reverse Transcription Kit (QIAgen, U.K.) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: RNA is reverse transcribed into complementary DNA (cDNA) using the QuantiTect Reverse Transcription kit (Qiagen©, UK) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA synthesis was carried out with 1 µg of RNA using the QuantiTect Reverse Transcription Kit (Qiagen) and subsequently diluted 1:25 in dH2O ...
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bioRxiv - Genomics 2023Quote: ... cDNA was synthesized through reverse transcription (RT) using the RT2 First Strand Kit (cat. no. 330401; Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was prepared from 1 μg of total RNA using a QuantiTect™ Reverse Transcription kit (Qiagen) from random hexamer primers ...
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bioRxiv - Cell Biology 2023Quote: ... Aliquots of 1 μg of RNA were reverse transcribed using a QuantiTect Reverse Transcription kit (Qiagen, 205314), and qPCR was performed with iTaq SYBR Green (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA synthesis from the purified RNA was performed using QuantiTect Reverse Transcription kit (Qiagen, catalog number 205311) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Approximately 900 ng of total RNA was retro-transcribed by using the QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µg of RNA was used per sample using the QuantiTect Reverse Transcription Kit (Cat# 205311, Qiagen). The quantitative RT-qPCR reactions were carried out using the PowerUp™ SYBR™ Green Master Mix (Cat# A25742 ...
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bioRxiv - Molecular Biology 2024Quote: ... Labchip analysis was performed to assess the size of small RNAs according manufacturer’s instructions (PerkinElmer).The Reverse transcription was performed on 4 ng RNA using miRCURY® LNA® RT Kit (Qiagen). Real-time quantitative PCR was performed on 40 pg cDNA using specific primers (Supplementary Table 1 ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized from 200 ng of total RNA with a QuantiTect Reverse Transcription Kit (205313, Qiagen). Quantitative PCRs were carried out in triplicate using SYBR Green I Master Mix (S-7563 ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA from the control human TSCs as well as GATA2-KD and GATA3-KD human TSCs were isolated using RNeasy Mini Kit (Qiagen, Germantown, MD) per the manufacturer’s protocol with on-column deoxyribonuclease digestion ...
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bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715, human YTHDF2: QIAGEN SI00764757, human YTHDF3: QIAGEN SI04133339, human METTL3: QIAGEN SI05020414 and human DDX6 ...
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bioRxiv - Genomics 2019Quote: ... and human genomic DNA (extracted from cells with DNeasy Blood & Tissue Kit, Qiagen). One guide was used with the plasmid ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were prepared using the QIASeq Human Comprehensive Cancer Panel Kit (Qiagen #333515) and sequenced on an Illumina HiSeq 2500 or NovaSeq 6000 ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted from human cells with an RNeasy Mini kit (Qiagen). Northern blot was performed as described previously (Tafforeau et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was extracted from human thyroid tissues using miRNeasy Mini kit (Qiagen) according to the manufacturer’s instructions and as described [26] ...
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bioRxiv - Neuroscience 2023Quote: ... Human rRNA depletion was performed using the QIASeq FastSelect kit (Qiagen, Hilden, Germany). RNA sequencing library preparation used NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s recommendations (New England Biolabs ...
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bioRxiv - Immunology 2023Quote: DNA from human stool samples was extracted with DNeasy PowerSoil Pro kit (Qiagen) according to the manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2022Quote: Total RNA for human TSCs was extracted using RNeasy Micro kit (Qiagen, 74004) with on-column DNase digestion ...
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bioRxiv - Synthetic Biology 2023Quote: ... or primary human cells were extracted using the Qiagen RNeasy Micro Kit (Qiagen). cDNA was produced using the SuperScript IV VILO cDNA Synthesis Kit (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... After 24 hours the media was removed for ELISA and the cells were lysed and RNA extracted (RNeasy Mini Kit, Qiagen).
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bioRxiv - Molecular Biology 2020Quote: ... Messenger RNA (mRNA) cDNA was synthesized using the QuantiTect® Reverse Transcription kit (Qiagen Inc., Germantown, MD, # 205313). Intron-spanning primers for mRNA were used to eliminate amplicons generated from any contaminating genomic DNA ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA of 500 ng was used for preparing cDNA libraries using QuantiTect Reverse Transcription Kit (Qiagen, USA). Gene expression was estimated by QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Plant Biology 2020Quote: ... DNase-treated RNA (2μg) was then reverse-transcribed in a total volume of 20μl using the Omniscript Reverse Transcription Kit (Qiagen).
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bioRxiv - Immunology 2021Quote: ... Five hundred nanograms of RNA was reverse transcribed into cDNA using the QuantiTect reverse transcription kit (Qiagen, # 205311). For one real-time reaction ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg total RNA was then used for first strand cDNA synthesis using QuantiTect Reverse Transcription Kit (Qiagen). Quantitative real-time PCR was performed using iQ SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... the first complementary strand was made from 1 µg total RNA using QuantiTect® reverse transcription kit (Qiagen). RT-PCR for the for gene expression analysis was carried out using the CFX-96 thermocycler (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... RNA isolation and reverse transcription: total RNA was extracted following the manufacturer’s recommended protocol (RNeasy Mini Kit, Qiagen). The quality and integrity of the total RNA were quantified by the NanoDrop DeNovix ...
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bioRxiv - Neuroscience 2022Quote: ... First strand cDNA synthesis from 1 µg of total RNA was performed with QuantiTect Reverse Transcription Kit (Qiagen). Quantitative RT-PCR experiments were performed with Rotor-Gene-Q machine (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: The reverse transcription was performed with 500 ng of total RNA using the miScript RT II kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... Reverse transcription of small RNAs was performed for all embryonic stages using the miRCURY LNA RT kit (Qiagen) using the recommended 10 ng total RNA input.
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bioRxiv - Molecular Biology 2020Quote: ... complementary DNA strand was synthesized from one μg of total RNA using QuantiTect® reverse transcription kit (Qiagen). Quantitative RT-PCR gene amplification was carried out using the CFX-96 thermocycler (Bio-Rad ...
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bioRxiv - Developmental Biology 2019Quote: ... The gRNA template for in vitro transcription was PCR amplified and purified with Qiaquick PCR purification Kit (Qiagen). In vitro transcription followed and was performed as described52 ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 µg of total RNA was subjected to cDNA synthesis using the QuantiTect Reverse Transcription Kit (Qiagen, 205314). Quantitative real-time PCR reactions were performed with the SYBR Select Master Mix (Life Technologies ...
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bioRxiv - Physiology 2019Quote: RNA was extracted from tissues using QIAzol and cDNA was synthesized using the Quantitect Reverse Transcription Kit (Qiagen). qPCR was conducted using Quantitect Sybr Green qPCR (Qiagen ...
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bioRxiv - Physiology 2021Quote: ... Complementary DNA (cDNA) synthesis was performed with 200–1,000 ng total RNA using QuantiTect_ Reverse Transcription Kit (Qiagen). Quantitative real time polymerase chain reaction (qRT–PCR ...
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bioRxiv - Microbiology 2020Quote: ... RNA (100 ng) was analyzed by reverse transcription-PCR (RT-PCR) using the OneStep RT-PCR kit (Qiagen). RNA was reverse transcribed and amplified in a total volume of 50 μl containing 2.5 mM MgCl2 ...
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bioRxiv - Microbiology 2020Quote: ... cDNA synthesis was performed from total RNA following the manufacturer’s instructions for the QuantiTect Reverse Transcription kit (QIAGEN). Gene silencing efficiency was determined using the SYBR Premix Ex Taq kit (Takara ...
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bioRxiv - Neuroscience 2022Quote: ... 500ng of RNA was used per biological replicate for cDNA conversion using QuantiTect reverse transcription kit (Qiagen, 205311). qPCR was run on a BioRad CFX96 using iTaq with SYBR Green (BioRad ...
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bioRxiv - Physiology 2022Quote: ... RNA (2000 ng) was reverse transcribed to cDNA using the QuantiTect Reverse Transcription Kit (Cat No. 205311, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... 500 ng of total RNA was used to synthesize first-strand cDNA using the Reverse Transcription Kit (Qiagen). For lncRNA expression profiling ...
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bioRxiv - Bioengineering 2023Quote: ... direct RT-qPCRs were performed on heat-inactivated samples without extraction using the QuantiTect Reverse Transcription Kit (Qiagen). The reactions were run in an Applied Biosystems QuantStudio 7 Flex System (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... An amount of 1 µg of RNA was reverse transcribed using Omniscript Reverse Transcription (RT) Kit (Qiagen, #205113) together with random oligonucleotides (Roche ...
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bioRxiv - Bioengineering 2023Quote: ... 1 μg of RNA per condition was used to retrotranscribe into cDNA QuantiTect® Reverse Transcription Kit (Qiagen). qPCR was performed in 386 wells plates in QuantStudio™ Real-Time PCR System (Applied Biosystems™ ...
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bioRxiv - Microbiology 2023Quote: Complementary DNA (cDNA) was synthesized from 500 ng DNAse-treated RNA using the QuantiTect reverse transcription kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Up to 1 ug of RNA was converted into cDNA using either the Quantitect Reverse Transcription kit (QIAGEN) or the Superscript IV transcription kit (ThermoFisher Scientific ...