Labshake search
Citations for Qiagen :
701 - 750 of 10000+ citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... Two μL of isolated RNA were used for reverse transcription (RT) and cDNA synthesis using the miRCURY LNA RT Kit (Qiagen) according to the manufacturer’s instructions adding the RNA spike-in UniSp6 as quality control for RT ...
-
bioRxiv - Molecular Biology 2023Quote: cDNA was produced from 1 μg of total RNA (final cDNA concentration 5 ng/ml) using QuantiTech Reverse Transcription kit (Qiagen) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2024Quote: ... reverse transcription quantitative PCR (RT-qPCR) assays were performed with the QuantiTect Virus + ROX Vial Kit (Qiagen, Toronto, ON, Canada) in a LightCycler® 480 system (Roche Molecular System) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Isolated RNA was retro-transcribed to cDNA using 1 μg of RNA per reaction with either the QuantiTect Reverse Transcription Kit (205311, QIAgen) or the High-Capacity cDNA Reverse Transcription Kit (4368813 ...
-
Notch3 is a genetic modifier of NODAL signalling for patterning asymmetry during mouse heart loopingbioRxiv - Developmental Biology 2024Quote: Reverse transcription was performed on RNA isolated from entire (left and right) micro-dissected heart fields using a Reverse Transcription kit (QuantiTect, Qiagen). Quantitative PCR was carried out using the ViiA7 real-time PCR system ...
-
bioRxiv - Genomics 2024Quote: ... Poly(A)RNA (500 ng) from each independent transfection was reverse transcribed to cDNA using QuantiTech Reverse Transcription kit (Qiagen) using a reporter-specific first strand primer (HJ7211 ...
-
bioRxiv - Cancer Biology 2024Quote: ... A total of 1 μg of total RNA from each sample was used to obtain double strand cDNA with the QuantiTect Reverse Transcription Kit (Qiagen). Quantitative Real-Time PCR (qRT-PCR ...
-
bioRxiv - Systems Biology 2020Quote: ... IL-8 ELISA was performed according to manufacturer instructions (Qiagen).
-
bioRxiv - Microbiology 2023Quote: Microbiome DNAs were extracted from the human samples on the same day of collection using the microbiome-specific kit (QIAamp DNA Microbiome Kit; Qiagen, Hilden, Germany). The DNA extraction was performed according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2021Quote: ... total RNA was extracted from tissues or cells as described above and reverse-transcribed using the QuantiTect Reverse Transcription Kit (Qiagen #205313). Quantitative real-time PCR (QRT-PCR ...
-
bioRxiv - Microbiology 2021Quote: ... cDNA was transcribed using reverse primer targeting the conserved termini of influenza gene segments and the Omniscript Reverse Transcription Kit (Qiagen, Germany). Each gene segment was amplified using segment-specific primers and extracted from 1% gel slices using Qiagen Gel Extraction Kit (Qiagen ...
-
bioRxiv - Evolutionary Biology 2020Quote: Up to 1 μg of RNA from each sample was converted into cDNA using a QuantiTect Reverse Transcription Kit and manufacturer’s directions (Qiagen, Hilden, Germany); a DNA-wipeout pre-step was included to avoid genomic contamination ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... First strand cDNA was reverse transcribed from 0.5 μg of total RNA using a Quantitect Reverse Transcription Kit (Qiagen, Hilden, Germany) that incorporates genomic DNA removal prior to reverse transcription ...
-
bioRxiv - Genomics 2020Quote: RNA extracted (~1μg) from patient nasopharyngeal swabs was used for double stranded cDNA synthesis using the QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany) according to manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2019Quote: Genes were quantified by one-step quantitative reverse transcription-polymerase chain reaction (q-PCR) using the QuantiFast SYBR Green RT-PCR kit (Qiagen, UK). A master mix was prepared by mixing 6.25 μl SYBR green ...
-
bioRxiv - Microbiology 2020Quote: ... 6 μl of each RNA sample were reverse transcribed using the Qiagen QuantiTect Reverse Transcription Kit according to the manufacturer’s instructions (Qiagen, Hilden, Germany). This protocol included an additional DNase treatment step and the reverse transcription reaction used a mix of random hexamers and oligo(dT ...
-
bioRxiv - Plant Biology 2020Quote: ... Aliquots of RNA (1 µg) from each sample were reverse transcribed to cDNA using the QuantiTect® Reverse Transcription Kit (QIAGEN), according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2019Quote: ... Synthesis of cDNA was performed on 0.3-1.0 μg of normalized total RNA from each sample using QuantiTect Reverse Transcription kit (Cat No. 205313, Qiagen, Valencia, CA) which included a DNase treatment step to remove any residual genomic DNA contamination ...
-
Global genetic patterns reveal host tropism versus cross-taxon transmission of bat BetacoronavirusesbioRxiv - Evolutionary Biology 2020Quote: ... All RNA extracts were subjected to reverse transcription polymerase chain reaction (RT-PCR) using the one-step RT-PCR kit (Qiagen, USA) and PanCoV F2 (5’-AAR TTY TAY GGH GGY TGG-3’ ...
-
bioRxiv - Pathology 2022Quote: ... RNA concentrations were determined spectrophotometrically and 1µg of total RNA was reverse transcribed using the QuantiTect Reverse Transcription Kit (Qiagen, Valencia, CA). Real-time RT-PCR was performed using a StepOne real time PCR system (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2022Quote: 1 µg of RNA was used for cDNA synthesis using the QuantiTect Reverse Transcription Kit according to the manufacturers’ protocol (Qiagen 205313). Quantitative PCR was carried out on a RotorGeneQ (Qiagen ...
-
bioRxiv - Microbiology 2019Quote: ... life technologies™) according to the manufacturer’s instructions before being reverse-transcribed using the QuantitTect® Reverse Transcription kit (Qiagen®). The resulting cDNA or serial dilutions of a known quantity of genomic DNA for generation of standard curves were amplified using a QuantiFast® SYBR® Green PCR kit (Qiagen® ...
-
bioRxiv - Pathology 2020Quote: ... Five hundred ng of RNA was reverse transcribed to cDNA using a reverse transcription RT2 First Strand Kit (Qiagen, Hilden, Germany) that is recommended to use in combination with RT2 Profiler PCR Arrays and we followed manufacturer’s protocol ...
-
bioRxiv - Physiology 2021Quote: ... an equal volume of RNA per reaction was DNase-treated and reverse transcribed using the QuantiTect Reverse Transcription Kit (Qiagen, 205314).
-
bioRxiv - Genetics 2020Quote: ... The RNA pellet was eluted in 15 μL of nuclease-free water and quantified on a Nanodrop 1000 spectrophotometer (NanoDrop Technologies) prior to DNAse treatment with QuantiTect® Reverse Transcription kit (Qiagen). The quality of the RNA was examined by electrophoresis on a 1% agarose gel (Sigma Aldrich) ...
-
bioRxiv - Physiology 2020Quote: ... One μg of total RNA was reverse transcribed into cDNA using the Qiagen QuantiTect Reverse Transcription Kit (Qiagen, Valencia, California, USA) following manufacturer’s protocols ...
-
bioRxiv - Genomics 2022Quote: ... The RNA pellet was eluted in 15 μL of nuclease-free water and quantified on a Nanodrop 1000 spectrophotometer (NanoDrop Technologies) prior to DNAse treatment with QuantiTect® Reverse Transcription kit (Qiagen). The quality of the RNA was examined by electrophoresis on a 1% agarose gel (Sigma Aldrich) ...
-
bioRxiv - Molecular Biology 2022Quote: PCR products produced as templates for in vitro transcription were purified with either the Qiaquick PCR Purification Kit (Qiagen, Hilden, Germany) for Cas9 templates ...
-
bioRxiv - Neuroscience 2022Quote: ... Complementary DNA (cDNA) was subsequently generated using 2 μg of total RNA and the miScript II Reverse Transcription (RT) Kit (Qiagen, Australia), according to the manufacturer’s instructions ...
-
bioRxiv - Pathology 2023Quote: ... RNA concentrations were determined spectrophotometrically and 1µg of total RNA was reverse transcribed using the QuantiTect Reverse Transcription Kit (Qiagen,Valencia, CA). Real-time RT-PCR was performed using a StepOne real time PCR system (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... RNA was normalised to the lowest sample concentration and cDNA libraries prepared using the QuantiTect® Reverse Transcription Kit (QIAGEN, UK) as per manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... The quality and quantity of the isolated total RNA were measured using a spectrophotometer and synthesised the cDNA using QuantiTect Reverse Transcription Kit (QIAGEN, Germany). Synthesized cDNA was kept at -80°C till further use.
-
bioRxiv - Cell Biology 2024Quote: ... 500ng total RNA were transcribed into complementary DNA using oligo-dT primers and OmniScript® Reverse Transcription kit (QIAGEN, Hilden, Germany). Transcription levels of genes of interest were assessed by quantitative PCR (qPCR ...
-
bioRxiv - Microbiology 2024Quote: ... and real-time reverse transcription quantitative PCR (qRT-PCR) was performed using QuantiNova™ SYBR Green PCR Kit (Qiagen, Hilden, Germany). Primers were synthesized by IDT Inc ...
-
bioRxiv - Cancer Biology 2021Quote: Total RNA from human and mouse kidney samples was harvested using the RNeasy Mini Kit (Qiagen). Total RNA isolation from cultured cells was extracted using Trizol reagent (Ambion ...
-
bioRxiv - Immunology 2021Quote: Genomic DNA was prepared from human or mouse B cells using QIAmp DNA Mini Kit (Qiagen), or from paraffin-embedded human IgD or IgA myeloma tissue sections (obtained from the University of Arkansas for Medical Science ...
-
bioRxiv - Cancer Biology 2020Quote: ... RNA from human cancer cells was extracted 48h post transfection using the RNeasy Kit from Qiagen according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2022Quote: Total RNA was isolated from murine and human monocytes with the RNeasy Plus Micro Kit (Qiagen), and then 200 ng of RNA was reverse transcribed using the iScript RT Supermix (Bio-Rad) ...
-
bioRxiv - Genomics 2022Quote: ... cfDNA was then extracted from human blood plasma using the QIAmp Circulating Nucleic Acids kit (Qiagen), eluted in 60-μl elution buffer (10 mM Tris-Cl ...
-
bioRxiv - Immunology 2020Quote: Total RNA was extracted from mouse or human whole blood using RNA blood mini kit (Qiagen). Isolated RNA was converted to complementary DNA (cDNA ...
-
bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from cultured human and mouse cells using an RNeasy Mini Kit (Qiagen) and included an on-column DNase treatment to eliminate contaminating genomic DNA ...
-
bioRxiv - Immunology 2022Quote: ... RNA was extracted from 5×106 human PBMCs from Cohort II using AllPrep RNEasy kits (Qiagen) and the small RNA-containing column flow-through collected ...
-
bioRxiv - Microbiology 2021Quote: Uninfected or infected primary human macrophages were subjected to RNA extraction using RNeasy Mini Kit (Qiagen) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... mouse organ lysates and human primary cell supernatant samples were prepared in RNeasy Mini Kit (Qiagen) lysis buffer RLT (400μl) ...
-
bioRxiv - Cancer Biology 2020Quote: ... human FLMs and adjacent normal tissue was performed using the AllPrep DNA/RNA Mini Kit (Qiagen). Whole exome sequencing was performed by Novogene using their standard protocols ...
-
bioRxiv - Pathology 2022Quote: ... Total RNA of mouse and human intestinal tissue was isolated using the RNeasy Mini Kit (Qiagen) and total RNA of mouse mesenteric fat was isolated using the RNeasy Lipid Tissue Mini Kit (Qiagen) ...
-
bioRxiv - Physiology 2023Quote: ... and grown to confluency and transfected with human Leptin constructs using Effectene kit (Cat# 301427, QIAGEN).
-
bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from human primary immune cells using the RNeasy Plus Micro Kit (Qiagen). RNA integrity number (RIN ...
-
bioRxiv - Cancer Biology 2023Quote: gDNA was isolated from human sarcoma models and controls using the QIAamp DNA Mini Kit (Qiagen). Library preparation was performed by the Functional Genomics Laboratory (FGL) ...
-
bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated from human sarcoma models and controls using the RNeasy Mini Kit (Qiagen). Library preparation was performed by the Functional Genomics Laboratory (FGL) ...