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Citations for Qiagen :
501 - 550 of 6183 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... The harvested contents were subjected to RT-PCR using OneStep Kit (210212, Qiagen, Hilden, Germany). Forward (F ...
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bioRxiv - Physiology 2023Quote: ... Quantitative real-time RT-PCR was performed in a Rotor-Gene Q (Qiagen, Hilden, Germany) using the 5x QPCR Mix EvaGreen (No Rox ...
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bioRxiv - Physiology 2021Quote: ... reaction tubes were transferred to a Rotor-Gene Q PCR thermal cycler for product amplification using a one-step protocol (QuantiFast SYBR® Green RT-PCR Kit, Qiagen, UK). The amplification protocol was as follows ...
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bioRxiv - Molecular Biology 2019Quote: ... using miScript Primer Assays for miRNAs and the using the QuantiTect SYBR Green PCR kit (Qiagen) for mRNA in a Stratagene Mx3005P qPCR machine (Agilent) ...
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bioRxiv - Genetics 2020Quote: ... Primer design was optimised to work with the Type-it Microsatellite PCR kit (QIAGEN, Hilden, Germany).
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bioRxiv - Synthetic Biology 2021Quote: ... with primers pBEST_LinL_F and pBEST_LinL_R (Table S1) and subsequent purification using QIAquick PCR Purification Kit (Qiagen).
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bioRxiv - Biochemistry 2020Quote: ... and pJET_Luc_FL_UTR_REV (5’-GCAATGAAAATAAATGTTTTTTATTAGGCAGAATCCAAATGC-3’) primers and was purified with the QIAquick PCR Purification Kit (Qiagen) directly after the PCR reaction ...
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bioRxiv - Immunology 2020Quote: Primer pairs were used in this study together with a QuantiFast SYBR green PCR kit (QIAGEN). The sequences of primer pairs used in this study are listed in Supplementary Table 3 ...
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bioRxiv - Immunology 2023Quote: ... and the purified pools were barcoded with Illumina indexing primers using a Multiplex PCR Kit (QIAGEN). All indexed pools for each sample were then pooled during column purification ...
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bioRxiv - Neuroscience 2020Quote: ... Those standards were generated by mixing commercially available unmethylated and hypermethylated DNA standards (EpiTect PCR Control DNA Set, Qiagen) resulting in 10 ng of total bisulfite-converted DNA ...
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bioRxiv - Immunology 2019Quote: ... RNA collected for real time PCR experiments was further purified using column DNA digestion (RNase-Free DNase set, Qiagen). cDNA was prepared using iScript cDNA-synthesis kit (Invitrogen ...
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bioRxiv - Systems Biology 2020Quote: ... the reactions were pooled in sets of four and purified on one column each (MinElute PCR purification kit, Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2019Quote: ... primers obtained from Qiagen (Quantitect Primer Assay, QT00101962) were used with an expected band length of 96bp ...
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bioRxiv - Cell Biology 2022Quote: The reverse primer was miScript Universal Primer (Qiagen).
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bioRxiv - Neuroscience 2020Quote: ... Primers (Qiagen): Gapdh QT00199633 ...
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bioRxiv - Plant Biology 2022Quote: ... RNase-Free DNase Set (Qiagen) was used to remove DNA ...
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bioRxiv - Immunology 2022Quote: ... RNase-free DNase Set (Qiagen) was used to remove potential DNA contamination ...
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bioRxiv - Genomics 2019Quote: ... and DNase set (79254; Qiagen), followed by delusion with 10 mL of RNase-free water ...
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bioRxiv - Neuroscience 2021Quote: ... dNTP set was from Qiagen. Plasmid purification kits were from ROBOKLON.
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bioRxiv - Plant Biology 2021Quote: ... RNase-Free DNase Set (Qiagen) was used according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2022Quote: ... RNase-Free DNase Set (QIAGEN) was used to remove all genomic DNA ...
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bioRxiv - Cell Biology 2024Quote: ... RNase-Free DNase Set (Qiagen) was used to remove genomic DNA contaminants ...
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bioRxiv - Molecular Biology 2021Quote: ... The blood samples from the 2020 FMD outbreak were analysed using a one-step PCR assay using the QIAGEN One-step RT-PCR Kit (Qiagen GmbH, Hilden, Germany) following the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2020Quote: An RNA probe targeting c-myb was generated using one-step RT-PCR (Qiagen, Manchester, UK) using the following primers c=myb-F 5’-CCAAGTCAGGAAAACGCCACCTCG-3’ and c-myb-R 5’-GCTGTTGTTTAGCGGAGTTGGGCT-3’ and cloned into the dual promoter vector pCRII-TOPO (Life Technologies) ...
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bioRxiv - Plant Biology 2022Quote: ... RT-qPCR was performed on cDNA using a Rotor-Q Real-Time PCR Cycler from Qiagen using gene-specific primers and normalized to the ACTIN gene (listed in Supplemental Table 1).
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bioRxiv - Cell Biology 2022Quote: ... Real time RT-PCR was performed using SYBR Green Fluor qPCR Mastermix (Qiagen, Limburg, The Netherlands) on a CFX Connect (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR of cDNA samples was performed using 2× Rotor-Gene SYBR Green PCR Mastermix (Qiagen) and Rotor-Gene Q real-time PCR cycler (Qiagen).
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bioRxiv - Pathology 2019Quote: ... Quantitative RT-PCR gene expression analysis was performed on duplicate samples using fast SYBR green (Qiagen) technology using the ViiA 7 Real-Time PCR System (Applied Biosystem) ...
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bioRxiv - Pathology 2021Quote: ... a RT-qPCR was performed as quality control step using miRCURY LNA miRNA PCR Assays (Qiagen) to test UniSp6 (Cat No ...
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bioRxiv - Microbiology 2020Quote: All qPCR and RT-qPCR-reactions were done using the QuantiTect Multiplex PCR NoROX Kit (Qiagen) or QuantiTect Probe RT-PCR Kit (Qiagen ...
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bioRxiv - Systems Biology 2021Quote: ... the presence of RVFV RNA was assessed using the QuantiNova® RT-PCR probe kit (QIAGEN). Samples were tested in duplicate using 5 μl of RNA template per well.
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bioRxiv - Plant Biology 2022Quote: ... RT-qPCR was performed with a 6plex real-time PCR system (Qiagen, Rotor Gen Q, Germany) using the RealQ Plus 2x Master Mix Green kit ...
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bioRxiv - Immunology 2024Quote: ... Cells were sorted directly into 12.5 μl (per well) of OneStep RT-PCR Buffer mix (QIAGEN) in a 96-well PCR plate (one cell/well).
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bioRxiv - Neuroscience 2021Quote: ... The Hsa-miR-124-3p miRCURY LNA miRNA mimic (Qiagen, Germantown, MD) was used at 15µM with miR-124 inhibitor ...
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bioRxiv - Evolutionary Biology 2019Quote: ... validated assay to target hsa-MIR-941 (Qiagen product# 339306, catalogue# YP00204574). Each 10µl reaction was made up of the following ...
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bioRxiv - Cancer Biology 2020Quote: ... were normalized to expression levels of hsa-miR-423-5p (Qiagen, YP00205624) to determine fold change.
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bioRxiv - Bioengineering 2020Quote: ... and linearly amplified with RT2 miR First Strand Kit (Qiagen, Hilden, Germany), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Pre-designed miR-511-3p inhibitors and mimics were purchased from Qiagen and transfection was carried out using the HiPerFect Transfection Reagent according to manufacturer’s protocol (Qiagen) ...
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bioRxiv - Genomics 2021Quote: ... The scramble-miR miRCURY LNA Detection probe (5’-GTGTAACACGTCTATACGCCCA-3’, YD00699004, QIAGEN) was used as a negative control ...
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bioRxiv - Cell Biology 2020Quote: ... hsa-miR-494-3p miRCURY LNA™ microRNA Mimic (YM00472106, MIMAT002816, Qiagen), miR-control (CAT#4464058) ...
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bioRxiv - Microbiology 2022Quote: ... with 4 × 108 copies of cel-miR-39 miRNA mimic (Qiagen 339390) spiked into to the sample after addition of lysis buffer.
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bioRxiv - Neuroscience 2024Quote: ... mice received a 0.5μM dose of a miR-505-5p mimic (Qiagen) or negative control combined with HiPerfect transfection reagent (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... with the spike-in control cel-miR-39-3p (QIAGEN, Hilden, Germany) and reverse transcribed (qScript microRNA cDNA synthesis kit Quanta Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... We performed purification between the two PCRs to reduce contaminants or carry-over of primer dimers using 96-well PCR purification plates (Qiagen Inc., Germantown, MD, USA). The final libraries were quantified with a Qubit Fluorometer (ThermoFischer ...
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bioRxiv - Immunology 2023Quote: ... The first round of PCR was carried out using the ImmunoSEQ proprietary PCR primer mix (32 μL per sample containing 25 uL of QIAGEN 2× Multiplex PCR Master Mix, 5μL of QIAGEN 5x Q-solution and 2 μL of primer mix). A positive control reaction ...
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bioRxiv - Neuroscience 2019Quote: ... The following primers from Qiagen (Qiagen Quantitect Primer Assay) were used:
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bioRxiv - Cancer Biology 2022Quote: ... The other primers were purchased from QIAGEN (Quantitect Primer): Hprt (QT00166768) ...
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bioRxiv - Neuroscience 2021Quote: ... Primers used were for Esr1 (QuantiTect Primer Assays; Qiagen), Chat ...
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bioRxiv - Cell Biology 2019Quote: ... All primers are QuantiTect primer from Qiagen (Hilden, Germany). Primers ...
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bioRxiv - Cancer Biology 2023Quote: ... Primers were purchased from Qiagen (miScript Primer Assay range) and from Eurogentec (primer list Supp ...