Labshake search
Citations for Qiagen :
451 - 500 of 1462 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2023Quote: ... pH 7.5 at 4°C) and purified using the RNAeasy kit (Qiagen).
-
bioRxiv - Immunology 2024Quote: ... and 4 weeks post-transduction using the DNeasy Blood & Tissue kit (Qiagen). Genomic DNA was also extracted from HEK293T cells used for lentiviral production to confirm baseline sgRNA frequencies ...
-
bioRxiv - Neuroscience 2024Quote: ... using a TissueLyser (2 × 30 Hz, 3 min at 4°C; QIAGEN). After homogenization ...
-
bioRxiv - Cancer Biology 2024Quote: ... TagLuc non-expressing clone 4 cells using the RNeasy Mini Kit (Qiagen). 1 μg was reverse-transcribed using an oligo-dT primer and ProtoScript II Reverse Transcriptase (NEB ...
-
bioRxiv - Cancer Biology 2021Quote: PBMCs were brought up to 15 mL volume in cold PBS and DNA was isolated with the DNeasy Blood & Tissue Kit (Qiagen catalog # 69504) according to the manufacturer’s protocol with 1 hour of incubation at 55°C for digestion ...
-
bioRxiv - Genomics 2021Quote: Total mRNA was extracted from ileum and bursa tissue (15-30 mg) using the Qiagen RNeasy mini kit (QIAGEN®, Hilden, Germany) following the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2022Quote: ... The remaining lysate was then transferred to 15 ml tubes containing 5 ml lysis buffer and 150 μl Ni-NTA agarose beads (Qiagen, Venlo, Netherlands) and incubated for 4 h at room temperature under constant mild agitation ...
-
bioRxiv - Physiology 2022Quote: ... total DNA was extracted from approximately 15 mg of muscle tissue and 300 mg of bone marrow using the QiAmp DNA mini kit (Qiagen, Valencia, CA). In subsequent experiments ...
-
bioRxiv - Pathology 2023Quote: ... spin column and proper buffer washing steps were carried out following manufacturer recommendations including a 15 min on-column DNAse (Qiagen, Germantown, MD) treatment ...
-
bioRxiv - Cancer Biology 2024Quote: ... and spun at 14000rpm for 15 mins followed by the isolation of aqueous phase and isolating RNA using the RNAeasy mini kit (Qiagen, Catalog # 74014). cDNA synthesis was performed using the iScript cDNA synthesis kit (Cat# 170-7691) ...
-
bioRxiv - Genomics 2024Quote: ... Cell debris was pelleted at 16,000g for 5 min and the supernatant was treated with 12µL of Proteinase K at 56°C for 15 min prior to purification with EZ1 DNA Tissue Kit (Qiagen; Cat. No. 953034) using the EZ1 Advanced XL machine (Qiagen) ...
-
bioRxiv - Systems Biology 2020Quote: ... and RNA seqFISH primary probe pools (1-10 nM per probe) and 10 nM polyT LNA oligo with a readout probe binding DNA sequence (Qiagen) were hybridized in 50% hybridization buffer consisted of 50% formamide (Invitrogen AM9342) ...
-
bioRxiv - Physiology 2021Quote: ... males n=11) and Ob (females n=10, males n=10) offspring using a commercially available kit (RNeasy Plus mini Kit, Qiagen) then reverse transcribed to cDNA (High Capacity cDNA Reverse Transcription kit ...
-
bioRxiv - Cell Biology 2020Quote: ... samples were centrifuged for 10 minutes at 20000 g at RT and pellets obtained pellets were resuspended in 10 μl of EB (Qiagen) shaking at 55°C for 10 minutes and finally purified with a PCR purification kit ...
-
bioRxiv - Biophysics 2022Quote: ... peak fractions were supplemented with 10 mM imidazole and 10 µg/mL aprotinin/leupeptin and bound to NiNTA beads (Qiagen) for 2 hours ...
-
bioRxiv - Genomics 2023Quote: ... The samples were rinsed with 4× SSC and hybridized with RNA seqFISH primary probe pools (1-10 nM per probe) and 10 nM polyT LNA oligo (Qiagen) in a 50% hybridization buffer consisting of 50% formamide (Invitrogen AM9342) ...
-
bioRxiv - Cell Biology 2020Quote: ... csa-1;csb-1 worms were exposed to 500 µM NR from the L4 stage and whole-body tissue was collected at adult day 7 for RNA extraction (Qiagen RNeasy mini kit with a QIAcube machine) [57] ...
-
bioRxiv - Plant Biology 2022Quote: ... 5’ and 3’ untranslated regions and open reading frames) for the paralogs were made using the CLC Main workbench 7 (QIAGEN® Aarhus A/S, Aarhus C, Denmark). Sequence alignments were generated for the genomic DNA ...
-
bioRxiv - Genetics 2020Quote: ... and eluted in 10 µl EB buffer (Qiagen). Libraries were sequenced in HiSeq4000 100-bp paired- end runs (Illumina ...
-
bioRxiv - Molecular Biology 2020Quote: ... 10 ml ATL lysis buffer (Qiagen, Hilden, Germany) supplemented with 2% Proteinase K (20 mg/ml ...
-
bioRxiv - Microbiology 2020Quote: ... 10 µl proteinase K (>600 mAU/mL, Qiagen) and 5 µl 20% sodium dodecyl sulfate solution were added ...
-
bioRxiv - Immunology 2021Quote: ... immersed in 10% formalin (Fisher) or RNAlater (Qiagen). Flash frozen tissues and those stored in RNAlater were stored at −80°C until further analysis.
-
bioRxiv - Cancer Biology 2023Quote: ... were transfected with either 10 µM control (QIAGEN) or esiRNA (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 10 μL DNase I stock solution (Qiagen) was then added to the column and incubated for 15 mins at RT ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2.7 x 10-05 AU/μL protease (Qiagen), and 1:8,000,000 diluted ERCC spike-in mix (Ambion) ...
-
bioRxiv - Molecular Biology 2024Quote: ... containing 10 μl QIAcuity probe Mastermix 4x (QIAGEN), 2 μl primer-probe assay 20x (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2024Quote: ... containing 10 μl QIAcuity probe Mastermix 4x (QIAGEN), 2 μl primer-probe assay 20x (Bio-Rad) ...
-
bioRxiv - Plant Biology 2024Quote: ... and immediately resuspended in 10 mL QIAzol (Qiagen). The suspensions were cleared by centrifugation (5 min ...
-
bioRxiv - Cell Biology 2020Quote: ... Clarified lysate was mixed with Ni-NTA agarose at 4℃ for 2h (Qiagen). Bound protein was eluted in 20 mM Tris pH 8.0 ...
-
bioRxiv - Developmental Biology 2021Quote: ... Lysates were treated with 4 μl of 100 mg/ml RNase A (QIAGEN) at 37 °C shaking at 500 RPM for 3 hours ...
-
bioRxiv - Immunology 2021Quote: ... and kept at 4°C RNA was purified using RNEASY Mini Kit (Qiagen) and its concentration determined using a Nanodrop 1000 ...
-
bioRxiv - Microbiology 2021Quote: ... at 4°C until RNA was extracted using the RNeasy Mini Kit (Qiagen)
-
bioRxiv - Microbiology 2020Quote: The MoBio PowerSoil-htp 96 kit (now Qiagen Cat No./Id: 12955-4), with minor modifications ...
-
bioRxiv - Cancer Biology 2020Quote: ... cell pellets were lysed in a 4% SDS buffer using a Qiashredder (Qiagen). Relative densitometry for western blots was determined using ImageJ software and normalized to the density of loading control β-ACTIN ...
-
bioRxiv - Microbiology 2021Quote: ... into a volume of 4 μL PBS sc 1X (Qiagen, Cat. No. 150345). To avoid evaporation of the PBS sc 1X during the sort ...
-
bioRxiv - Genomics 2021Quote: ... 4 μL of room temperature Stop solution (REPLI-g Single Cell Kit, Qiagen) was then added and the samples were vortexed and spun down ...
-
bioRxiv - Molecular Biology 2023Quote: ... Effectene (4 μL of enhancer and 5 μL of Effectene reagent; Qiagen 301427) was used to transfect 500 ng of the indicated eGFP plasmid ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 18 µl of Omniscript enzyme at 4 U/µl (Omniscript RT Kit, Qiagen), and 37 µl water ...
-
bioRxiv - Genomics 2023Quote: Approximately 3-4 million cells were solubilized with 1 mL QIAzol (Qiagen, 79306) and 0.2 mL chloroform in 5PRIME Phase-Lock Gel heavy tubes (QuantaBio ...
-
bioRxiv - Biophysics 2022Quote: ... The supernatant was incubated overnight at 4 °C with Strep-Tactin Beads (Qiagen), typically using 0.75 ml packed beads per two liters of original culture volume ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were transfected with dsHsc70-4 with the Effectene Transfection reagent (301427, QIAGEN) and incubated for 72 h ...
-
bioRxiv - Microbiology 2023Quote: ... supernatant was incubated overnight at 4°C with Ni-NTA agarose beads (Qiagen) used to purify the recombinant proteins as described [40] ...
-
bioRxiv - Neuroscience 2023Quote: Cultured cells were lysed at 4°C in RLT buffer (Qiagen, Courtabœuf, France) and stored at -80°C ...
-
bioRxiv - Microbiology 2024Quote: ... DNA was isolated with the DNeasy UltraClean 96 Microbial Kit (Qiagen 10196-4). Library preparation and sequencing were performed at the NGS Competence Center NCCT (Tübingen ...
-
bioRxiv - Genomics 2024Quote: ... with 4 µl of RNase A (100 mg/ml) (Qiagen, Germantown, MD, USA), The extraction procedure was then followed by the manufacturer’s manual ...
-
bioRxiv - Synthetic Biology 2024Quote: ... cells were harvested by scraping in 4 mL lysis buffer (RLT buffer (Qiagen) + 143 mM β-ME) ...
-
bioRxiv - Biochemistry 2024Quote: ... The soluble fraction was then added to 4 mL Ni-NTA beads (Qiagen) pre-washed in water ...
-
bioRxiv - Genetics 2024Quote: ... Samples were cooled and 4 µL 100 mg/mL RNAse A (Qiagen, #19101) was added ...
-
bioRxiv - Developmental Biology 2024Quote: ... 10 katna1-/-MZ and one pool of 10 wild-type embryos (at 24 hpf) using the RNeasy Mini-kit (Qiagen, France) and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen ...
-
bioRxiv - Cancer Biology 2024Quote: ... Invasive carcinoma in the ovary and metastatic sites were macrodissected and DNA extracted from 10 × 10 μm sections using QIAmp DNA FFPE Tissue Kit (Qiagen, UK).