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Citations for Qiagen :
701 - 750 of 1462 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Total RNA of spleens (4 samples per group) was isolated with the QIAGEN miRNeasy mini extraction kit (QIAGEN) and cDNA was synthesized with the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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Deconstructing the role of iron and heme in the structure of healthy human gut microbial communitiesbioRxiv - Microbiology 2022Quote: ... 50 µL of saturated bacterial cultures were extracted using a DNeasy UltraClean 96 Microbial Kit (Qiagen, 10196-4).
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Deconstructing the role of iron and heme in the structure of healthy human gut microbial communitiesbioRxiv - Microbiology 2022Quote: ... PCR products were individually cleaned up and quantified using the UltraClean 96 PCR Cleanup Kit (Qiagen 12596-4) and the Quant-iT dsDNA High Sensitivity Assay kit (Invitrogen Q33120 ...
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bioRxiv - Biophysics 2022Quote: ... The suspension was spun down at 16,000 r.c.f at 4 °C for 30 min and the supernatant was applied 5 ml Ni-NTA resin (Qiagen)/ L culture medium ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 30 min at 45,000 × g at 4°C and incubated with 0.6 ml of Ni-NTA agarose beads (Qiagen) per 1 l of expression culture (1 ml beads per 1 l expression culture was used in case of Drosophila BiP ...
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bioRxiv - Genetics 2020Quote: ... 4 dpe on-tet n=3) was extracted using the Qiagen RNeasy Mini Plus Kit (Qiagen, Hilden, Germany), which includes a column-based genomic DNA removal step ...
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bioRxiv - Cancer Biology 2021Quote: ... 1.28 M sucrose, 40 mM Tris-Cl, pH 7.5, 20 mM MgCl2, and 4% Triton X-100; QIAGEN) and then digested by digestion buffer (QIAGEN buffer G2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μl of diluted cDNA template was mixed with 4 μl QuantiTect SYBR Green PCR Master Mix (Qiagen), 100 nM forward primers (see Table 1 for sequences ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were lysed (1.28 M sucrose, 40 mM Tris-HCl [pH 7.5], 20 mM MgCl2, and 4% Triton X-100; Qiagen) and digested (800 mM guanidine–HCl ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from cSCC cell lines (n = 8) and NHEKs (n = 4) using miRNeasy Mini Kit (Qiagen), and the RNA-seq analysis was performed using Illumina HiSeq2500 system using paired-end sequencing chemistry with 100bp read length (Illumina ...
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bioRxiv - Biochemistry 2023Quote: ... for 45 min at 4°C and the supernatant was mixed with 3 mL Ni-NTA agarose (Qiagen) pre-equilibrated in lysis buffer ...
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bioRxiv - Microbiology 2022Quote: ... 14 μL of filtered supernatant was combined with 4 μL of RDD buffer (Qiagen, RNase-Free DNase Set) and 2 μL of DNase I and dissolved as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... 4 °C for 1 h and purified by affinity chromatography using a 5 ml Ni-HP column (Qiagen). Flow through with pure AstaPo1 was collected and dialyzed overnight against the buffer containing 50 mM Tris pH 7.6 ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% CA630) and rotated at 4°C for 2 hours before addition of His-beads (Ni-NTA, QIAGEN). After continuous rotating for another 2 hours ...
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bioRxiv - Molecular Biology 2023Quote: We also used the RT2 Profiler PCR array mouse fibrosis (cat. #PAMM 120-ZE-4) kit from Qiagen to detect the expression of 84 fibrosis-related genes ...
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bioRxiv - Developmental Biology 2023Quote: ... Plates were then placed at 4 0C before adding the reverse transcription mix containing 5’-biotinylated TSO (Qiagen). PCR products were cleaned up using 0.8:1 Ampure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria were centrifuged at 4°C and washed with pre-cold PBS containing RNA stabilization solution (RNAprotect, Qiagen) before flash freezing of the pellets and storage at -80°C ...
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bioRxiv - Biophysics 2024Quote: ... for 4 hours at 37°C and purified from a 2% agarose gel (QIAquick Gel Extraction Kit, Qiagen). At this stage ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was the incubated at 4 °C with 2 ml of washed Ni-NTA agarose beads (Qiagen) for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... for 45 min at 4°C and the supernatant was mixed with 3 mL Ni-NTA agarose (Qiagen) pre-equilibrated in lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... coli 10-beta cloning strain were purified using the standard QIAprep protocol (QIAGEN), then delivered to the Pseudomonas strain via previously published electroporation83 or conjugation84 protocols ...
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bioRxiv - Genomics 2020Quote: Total RNA was isolated from 10-20 mg heart tissue (RNeasy kit, Qiagen) of PN and AH mice (n≥6 in each group) ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting eluate was treated with RNase A (10 μg; Qiagen, Hilden, Germany) for 1 hr at 37 °C and Proteinase K (4 U ...
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bioRxiv - Cell Biology 2021Quote: ... 5×10^5 EpCAM+ve cells were lysed in 350μL RTL Buffer (Qiagen) containing 143mM β-Mercaptoethanol (aided by cell searing via passage ...
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bioRxiv - Cell Biology 2020Quote: ... 20 μl of 10% SDS and 5 ul of Proteinase K (Qiagen #19131) were added to each sample ...
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bioRxiv - Plant Biology 2022Quote: ... and the leaves were homogenized on 10 mM MgCl2 buffer using TissueLyserll (Qiagen) with the frequency of 30 s-1 for 5 min ...
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bioRxiv - Developmental Biology 2022Quote: 10 embryos were collected into QIAzol Lysis Reagent (Qiagen, Cat. No. / ID: 79306), homogenized and centrifuged ...
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bioRxiv - Neuroscience 2020Quote: ... 10 μL.mL−1 NP-40) and immediately placed in “RTL plus” buffer (Qiagen). mRNA was purified using RNeasy Plus Micro Kit (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... 20 μl of 10% SDS and 5 ul of Proteinase K (Qiagen #19131) were added to each sample ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cells were incubated in either 10 nM of LNA enhanced detection probes (Qiagen) targeting mRNAs for I-RIM ...
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bioRxiv - Molecular Biology 2021Quote: 10 μg RNA was cleaned up using the RNeasy Miniprep Kit (Qiagen, 74106) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... then 10 μL of DNase + 70 μL of buffer RDD (Qiagen, Cat # 79256) were added to the column and incubated for 15 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... with 10 μL/mL β-mercaptoethanol and mechanically disrupted with a TissueRuptor (Qiagen), before RNA extraction ...
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bioRxiv - Cell Biology 2021Quote: ... Fractions were eluted using 10 mM reduced glutathione through a polypropylene column (Qiagen), and those with the highest concentrations combined ...
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bioRxiv - Immunology 2020Quote: ... and 1,000 CFSE-negative cells were sorted into 10 μL TCL buffer (Qiagen) with 1% BME (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were transfected with 10 µg plasmid DNA using Effectene transfection reagent (Qiagen). After an additional 24 hours ...
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bioRxiv - Physiology 2023Quote: ... or from 10 dissected fat bodies using the QIAGEN RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... reaction mixes were prepared with 10 μL QuantiTect Probe PCR master mix (QIAGEN), 1.0 μM primer and 0.25 μM probe ...
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bioRxiv - Biochemistry 2024Quote: ... cells were transfected in 10 cm2 at ∼60% confluency using PolyFect (Qiagen, 301107) and washed with complete culture medium 6 h later ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 10 fly heads per condition were homogenized in Qiazol (Qiagen) and total RNA was isolated ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was mixed with 10% (v/v) Ni-NTA agarose beads (Qiagen) preequilibrated with the lysis buffer for 40 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Filtered cell lysate was applied to 10 mL of Ni-NTA resin (Qiagen) which had been previously equilibrated with 10 column volumes of H2O ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysate was loaded on a 10 ml Ni-NTA Superflow column (QIAGEN), washed with 7 column volumes of 20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... 10 000 cells were directly sorted in 350 µL of RLT buffer (Qiagen), vigorously vortexed for disruption and spin down ...
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bioRxiv - Cancer Biology 2023Quote: ... consisting of 10 μl of 2× QuantiNOVA SYBR Green PCR master mix (Qiagen), 7.2 μL nuclease-free water ...
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bioRxiv - Genetics 2022Quote: ... 10 μg dsRNA were transfected into 3×106 Drosophila cells using Effectene (QIAGEN).
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bioRxiv - Physiology 2024Quote: ... at 10 U/μ purification using the QIAprep Spin MINI Prep Kit (Qiagen), the DNA was transcribed in vitro into RNA by T7 polymerase and then transfected into HeLa cells using Lipofectamine (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... Leaf disks were ground under freezing conditions using 10 mm tungsten beads (Qiagen) in the Genogrinder at 1000 rpm for one minute ...
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bioRxiv - Biochemistry 2024Quote: ... the protein was again loaded onto a NiNTA 10/50 column (Qiagen, Germany) and subsequently subjected to size exclusion chromatography using Superdex 200 prep grade column (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... 20 mM Imidazole) and incubated with 10 µL of Ni-NTA agarose (Qiagen) or 10 µL of anti-FLAG M2 affinity gel at 4°C for 1.5 h or overnight ...