Labshake search
Citations for Qiagen :
401 - 450 of 1462 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... Triplicate PCRs were carried out in 15 µl reactions containing 1x UCP Multiplex PCR Master Mix (Qiagen, Venlo, The Netherlands), 0.3 μmol l−1 each of the forward and reverse primers ...
-
bioRxiv - Molecular Biology 2023Quote: ... Ligated fragments were then amplified for 12-15 cycles using primers incorporating unique dual index tags with VeraSeq polymerase (Qiagen). Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases.
-
bioRxiv - Cancer Biology 2024Quote: ... the samples were eluted at this stage by using the Dovetail® kit 1xReversal of crosslinks salt buffer and proteinase K incubated at 55 degrees for 15 min and 68 degrees for 45 min and purified using the PCR Purification kit from Qiagen.
-
bioRxiv - Systems Biology 2020Quote: ... the MoBioPowersoil 96 kit (now Qiagen Cat No./Id: 12955-4) was used with minor modifications ...
-
bioRxiv - Genomics 2021Quote: ... 4 μL of Buffer D2 (REPLI-g Single Cell Kit, Qiagen) and 1 μL of 500 μM exonuclease-resistant random primer were then added to the lysed cells to denature the DNA prior to vortexing ...
-
bioRxiv - Cell Biology 2022Quote: ... and TBT (4 biological replicates per condition) with RNeasy columns (Qiagen), followed by oligo-dT selection ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated on 4-12% Bis-Tris gels (Novex, Qiagen) using MOPS running buffer (Novex ...
-
bioRxiv - Molecular Biology 2024Quote: ... the MagAttract PowerMicrobiome DNA/RNA extraction kit (Qiagen 27500-4-EP) and for 16 samples ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 10 μL of the RDD buffer (Qiagen), 2.5 μL of DNase I (Qiagen ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 10 μl of HotStarTaq Master Mix (Qiagen), 0.08 μM of each primer ...
-
bioRxiv - Genetics 2022Quote: ... for 10 minutes using a TissueLyserLT (Qiagen), then incubated on ice for 30 minutes ...
-
bioRxiv - Genomics 2023Quote: ... 10 µL of RLT+ lysis buffer (Qiagen) was then added to each well and the plate was sealed and centrifuged (2000 x g ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2.7 × 10−5 AU/mL protease (Qiagen), 1.0 μM barcoded oligodT-ISPCR primer ...
-
bioRxiv - Microbiology 2024Quote: ... 10 μg/ml RNase-free DNase (Qiagen), and 1 uL/ml of RNaseOUT (Thermo Fisher) ...
-
bioRxiv - Microbiology 2024Quote: ... 10 ml of RNAprotect Bacteria Reagent (Qiagen) was added to cultures ...
-
bioRxiv - Microbiology 2020Quote: ... whereas 30 mg of lung tissue was homogenized in 600 μl RLT-buffer containing β–mercaptoethanol for 30 sec at 15 Hz using the TissueLyser II (Qiagen).
-
bioRxiv - Evolutionary Biology 2020Quote: We extracted genomic DNA from pools of 60 whole adult males and 15 whole virgin adult females using DNeasy Blood and Tissue kit (Qiagen, CA) and Promega DNA Clean and Prep Kit (Promega ...
-
bioRxiv - Microbiology 2021Quote: ... Suspended fecal samples and swab samples were centrifuged at 4,000 x g for 15 min and viral RNA was extracted from each supernatant using the QIAamp Viral RNA Mini Kit (Qiagen, USA). Conventional RT-PCR was performed using the Invitrogen™ SuperScript™ IV First-Strand Synthesis System (Thermo Scientific ...
-
bioRxiv - Cell Biology 2021Quote: Whole DNA was extracted from 15-25 mg of mouse tissue using the DNeasy Blood and Tissue Kit (QIAGEN, cat# 69504) according to the kit’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Quantitative RT-qPCR were performed using primers previously described by Dias de Melo et al [15] using QuantiNova SYBR® Green RT-PCR Kit (Qiagen) according to the manufacturer instructions ...
-
bioRxiv - Immunology 2021Quote: ... according to the product instructions and underwent 15 min on-column DNase digestion using an RNase-Free DNase Set (Qiagen, 79254). cDNA was prepared with reverse transcriptase using iScript kit (BioRad Laboratories ...
-
bioRxiv - Microbiology 2020Quote: ... and then with (2) 15 µl Proteinase K [>600 mA/ml] and 5 µl Rnase A [100 mg/ml] (Qiagen, Germany) for 5min at room temperature ...
-
bioRxiv - Neuroscience 2021Quote: ... The isolated RNA was eluted in 15 μl of elution buffer following on column DNAse1 treatment using RNase-free DNase set (Qiagen # 79254). The total RNA was assessed for RNA integrity with a bioanalyzer (Agilent ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA extractions were performed on cell pellets from 15 mL of these back-diluted cultures using QIAprep Spin Miniprep Kit (Qiagen, 27104).
-
bioRxiv - Systems Biology 2024Quote: ... The samples were then cooled for 5 minutes on ice and transferred to a pre-spun 15 mL MaXtract tube (Qiagen 129065) containing an equal volume of chloroform to the acid phenol used ...
-
bioRxiv - Microbiology 2024Quote: ... were centrifuged at 4,000 × g for 15 min and viral RNA was extracted from each supernatant using the QIAamp Viral RNA minikit (Qiagen, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The samples were then transferred to a 15 ml tube and processed with a Qiagen Midi Prep Kit (Qiagen, Valencia, CA) following the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2022Quote: ... A low-pressure chromatography column connected to a peristaltic pump (2 mL/min flow rate) was prepared with 15 mL nickel resin (Qiagen #30230), followed by equilibration with 50 mL Nickel A Buffer ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The complete set of 15 loci were amplified in four separate 15 μL multiplex-PCR reactions containing 7.5 μL of 2X Master Mix (Type-It Microsatellite PCR kit, Qiagen, Venlo, Netherlands), 0.015-0.15 μL of unlabeled forward primer with tail (10 μM) ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Total RNA was extracted using an RNeasy Mini kit with on-column DNase I digestion for 15 min (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2024Quote: Reverse transcription primers with a length of 15-25 nucleotides were designed using CLC Main Workbench software (CLC Bio workbench version 7.6, QIAGEN, Hilden, Germany) based on the guidelines published by Weibrecht et al ...
-
bioRxiv - Cell Biology 2024Quote: ... approximately 15-20 mg of mouse intestine tissue were used for RNA purification using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Pathology 2024Quote: ... The lyophilized pellets were ground into a fine powder using ∼15 glass beads in a grinding machine (Qiagen MM300 Retsch TissueLyser). A total of 560 DNA samples of isolates coming from separate leaves were extracted using an automated DNA extraction robot ...
-
bioRxiv - Molecular Biology 2024Quote: ... was extracted from 15 mg (dry weight) of cardiac tissue using the DNeasy Blood & Tissue DNA extraction kit (69504, Qiagen, UK). The DNA concentration was measured using the PicoGreen assay (P7589 ...
-
bioRxiv - Molecular Biology 2020Quote: ... After 14 h binding to 4 mL Ni-NTA agarose beads (Qiagen), the beads were first washed with 15 mL Ni-NTA binding buffer and then with 15 mL Ni-NTA wash buffer (1 M NaCl ...
-
bioRxiv - Biochemistry 2021Quote: ... 4 µL of DNA) and run in Rotor-Gene Q machine (QIAGEN). Primer sequences are listed in the table S2 ...
-
bioRxiv - Bioengineering 2021Quote: ... Sample supernatants were incubated with 4 mL of Ni-NTA Agarose (Qiagen) for >1 hour at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... and pooled using the UltraClean 96 PCR Cleanup kit (Qiagen 12596-4) and Quant-iT dsDNA High Sensitivity Assay kit (Invitrogen Q33120) ...
-
bioRxiv - Microbiology 2022Quote: ... and pooled using the UltraClean 96 PCR Cleanup kit (Qiagen 12596-4) and Quant-iT dsDNA High Sensitivity Assay kit (Invitrogen Q33120) ...
-
bioRxiv - Genomics 2022Quote: ... and 4 µl of 100 mg/ml RNase A (QIAGEN, cat# 19101) were added to the homogenate ...
-
bioRxiv - Immunology 2022Quote: ... After diluting samples in a 4:1 ratio (elution buffer [Qiagen]:cDNA), cDNA concentration was determined using a Bioanalyzer (Agilent Technologies).
-
bioRxiv - Biochemistry 2020Quote: ... Crystallization screening was performed at 4°C with JCSG core I (Qiagen) using the sitting drop vapor diffusion method ...
-
bioRxiv - Cell Biology 2020Quote: ... 4 μl of DNA) and run in Rotor-Gene Q machine (Qiagen). Primer sequences are listed in the Table 2 ...
-
bioRxiv - Microbiology 2021Quote: ... Removed culture was mixed with 4 mL of RNAprotect Bacteria Reagent (Qiagen) for stabilization and incubated for 5 min at room temperature before centrifugation at 4000 rpm for 10 min ...
-
bioRxiv - Microbiology 2022Quote: ... with 4 × 108 copies of cel-miR-39 miRNA mimic (Qiagen 339390) spiked into to the sample after addition of lysis buffer.
-
bioRxiv - Neuroscience 2022Quote: ... using a TissueLyser (2 × 30 Hz, 3 min at 4°C; QIAGEN). After homogenization ...
-
bioRxiv - Molecular Biology 2022Quote: ... HL-1 cells were transduced with a pool of 4 gapmers (Qiagen) at 40nM (10Nm each ...
-
bioRxiv - Neuroscience 2023Quote: ... At least 4 colonies were used for each DNA miniprep (QIAprep, Qiagen) and mutations were confirmed by Sanger sequencing (Source Bioscience) ...
-
bioRxiv - Microbiology 2023Quote: ... The siRNAs were selected from the FlexiTube GeneSolution 4 siRNA sets (Qiagen) and transfected as a mix at 24 nM in Calu-3 and 10 nM in Caco-2 following manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... at a frequency of 28/s @ 4°C (Tissue Lyser II, Qiagen). After this step the samples were adjusted to RT for 5 minutes and processed using the RNeasy Lipid Tissue Mini Kit (Qiagen ...