Labshake search
Citations for TianGen Biotech :
401 - 450 of 548 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The reverse transcription of miRNA was performed with miRcute Plus miRNA First-Strand cDNA Kit (Tiangen, Beijing, China) and mRNA reverse transcription was performed with the PrimeScript RT Reagent Kit with gDNA Eraser (Takara ...
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bioRxiv - Genetics 2022Quote: ... the cells were collected with sufficient material for DNA isolation with a TIANamp Genomic DNA Kit (DP304, TIANGEN) for genotyping analysis in technical triplicate for genotyping analysis ...
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bioRxiv - Plant Biology 2022Quote: Plant total RNA was extracted using an RNA-simple Total RNA Kit (Tiangen Biotech Co., Ltd., Beijing, China). DNA contamination in the RNA sample was removed by 4xgDNA wiper (Vazyme Biotech Co. ...
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bioRxiv - Genomics 2024Quote: ... A cDNA library was constructed using the TIANSeq Fast RNA Library Prep Kit (Cat. no: 4992376; Tiangen, China) and then sequenced using an Illumina NovaSeq6000 sequenator (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted from each cell culture flask with Plant genomic DNA kits (Tiangen BioTech., Beijing, China). Genomic PCR (gPCR ...
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bioRxiv - Molecular Biology 2023Quote: ... Nucleic acid extraction from animal tissue grinding fluid (200μL) was performed using a DNA extraction kit (TIANGEN, China) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... the DNA of successfully transfected cells or AAV8 treatment tissues were extracted with TIANamp Genomic DNA Kit(TIANGEN,) according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid purification and extraction steps were carried out using the Endo Free Mini Plasmid Kit II (DP118, TIANGEN). ShRNA sequences are detailed in supplementary Figure 1 ...
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bioRxiv - Plant Biology 2024Quote: ... The amplified products were recovered from an agarose gel using a Universal DNA Purification Kit (TIANGEN, DP214, China). The purified product was cloned into the pBM16A vector using a pBM16A Topsmart Cloning Kit (BIOMED ...
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bioRxiv - Immunology 2024Quote: RNA was extracted from FACS-sorted NK cells or splenocytes using RNASimple Total RNA Kit (TIANGEN Biotech, 4992858) and subsequently reverse transcribed to cDNA with SuperScript VILO Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: Total DNA from each sample was extracted using Tiangen DNA Stool Mini Kit (Tiangen Biotech Co., Ltd., China). Metagenome sequencing was performed using the Illumina NovaSeq 6000 platform with a paired-end sequencing length of 150 bp ...
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bioRxiv - Molecular Biology 2020Quote: ... and dH2O in a 25 μl volume using a QuantiTect SYBR Green PCR kit(TIANGEN BioTech Co.LTD, Beijing, China).Transcripts were quantified with β-actin as an internal standard ...
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bioRxiv - Evolutionary Biology 2020Quote: We extracted genomic DNA from fresh or silica gel-desiccated leaves with a DNAsecure Plant Kit (TIANGEN, Beijing, China). The primers and thermal cycling procedure were as described by Craig et al (2014) ...
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bioRxiv - Genetics 2021Quote: High-quality genomic DNA was isolated from fresh leaves using the Plant Genomic DNA Kit (Tiangen Biotech, Beijing, China). The DNA of 119 RILs and two parental lines was subsequently genotyped by CapitalBio Technology (Beijing ...
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bioRxiv - Plant Biology 2021Quote: ... Soil DNA (sDNA) extraction from each subsample was performed directly using the Soil DNA Mini Kit (Tiangen, Beijing, China). The sDNA was diluted to a concentration of 2 ng ml-1 before addition to the LAMP reaction ...
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bioRxiv - Physiology 2020Quote: The total RNA content in leaves was extracted using the Total RNAsimple Kit (Tiangen Biotech Co., Ltd, Beijing, China) in accordance with manufacturer’s guidelines ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from the treated microbial cells with a microbial genomic DNA extraction kit (Tiangen Biotech, Beijing, China) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2022Quote: ... RT–qPCR was performed via a miRcute plus miRNA qPCR kit (SYBR Green) (Tiangen Biotech Co., Ltd., Beijing, China) on an ABI 7500 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Zoology 2020Quote: ... The DNA extractions from tissue samples were performed using the TIANamp Genomic DNA Kit (TIANGEN BIOTECH CO., LTD, China) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... total RNA was extracted from bat intestine tissue using the RNAprep pure Kit (for Cell/Bacteria) (TIANGEN, Beijing, China). First-strand complementary DNA was synthesized from total RNA by reverse transcription with random hexamers ...
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bioRxiv - Neuroscience 2022Quote: ... 2 ug RNA per sample were used to reverse transcript the cDNA by QuantScript RT Kit (TIANGEN Biotech, KR103). 2 x iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA from the seeds was isolated using the RNAprep Pure Plant Kit (Polysaccharides and Polyphenolics, Tiangen, Beijng, China) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... total RNAs were extracted from seedlings or different plant tissues by RNA Easy Fast Plant Tissue Kit (TIANGEN, DP452) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA of 3-week-old SsSINA1a-overexpressing Arabidopsis was extracted using an RNA Extraction Kit (DP411, Tiangen Biotech). The housekeeping genes AtACTIN was used as the internal control ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA extraction from each sample employed the RNA Easy Fast Cell Total RNA Extraction Kit (DP451, Tiangen, Beijing), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted by using an RNA Prep Pure Plant Kit (polysaccharide and polyphenolic-rich) (TIANGEN, Beijing, China) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: Genomic DNA was isolated from the iPS cell line using the Magetic Univeral Genomic DNA kit (TIANGEN, DP705-01). Copy numbers of ampliconic genes were validated through droplet digital PCR (ddPCR) ...
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bioRxiv - Zoology 2023Quote: ... total genomic DNA was extracted from muscle tissue of pre-thoraxes using Tissue DNA kit (TIANGEN Biotech, Beijing, China) according to the manufacture’s protocol ...
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bioRxiv - Genomics 2023Quote: ... the genomic DNA was extracted from young leaves using the Hi-DNAsecure Plant Kit (cat. no. DP350; TIANGEN, China). Then ...
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bioRxiv - Cancer Biology 2024Quote: ... The K20R and K20Q mutant AKT1 plasmids were constructed using the Fast Site-Directed Mutagenesis Kit (TIANGEN, Cat# KM101). All plasmids used in this article were confirmed by Sanger sequencing.
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bioRxiv - Genetics 2024Quote: The genomic DNA from blood of each newborn piglet was extracted using TIANamp genomic DNA kit (Tiangen, Beijing, China) and PCR was carried out using 2×Ex Taq Master Mix (TaKaRa CW0718) ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized using 1 µg of total RNA by utilizing the FastQuant RT Kit (with gDNase) (TIANGEN KR106). Synthesized cDNAs were then quantified by qRT‒PCR with PerfectStart® Green qPCR SuperMix (TRAN AQ601 ...
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bioRxiv - Microbiology 2024Quote: ... The total RNA of all the samples was extracted using RNAprep Pure Cell/Bacteria Kit (Tiangen Biotech, Beijing, China) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA from these 50 samples was extracted from leaves using the Magnetic Plant Genomic DNA Kit (Tiangen, China). Paired-end libraries were constructed for all samples ...
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bioRxiv - Molecular Biology 2020Quote: Experimental cells were subjected to isolation of total RNAs by using the RNA simple Kit (Tiangen Biotech Co., Beijing, China). Then ...
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bioRxiv - Genetics 2021Quote: Genomic DNA was extracted from the wings of Bmmp knockout silkworms at the adult stage using the TIANamp Genomic DNA Kit (TIANGEN). The DNA was used as a PCR template to amplify regions spanning the genomic targets ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA from the different tissue and leaf axil specimens was extracted with an RNAprep Pure Plant Kit (TIANGEN, China) according to the kit protocol ...
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bioRxiv - Neuroscience 2021Quote: ... All DNA products were separated in agarose gel to confirm sizes and following purified using TIANgel Midi Purification Kit (TIANGEN). The mixture of 0.01 pM of backbone and 0.03 pM of each insert fragments were delivered for transformation in Stable3 competent bacteria after 30 minutes incubation in 50 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... The DNA fragments were separated by agarose gel electrophoresis and purified using a TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Microbiology 2020Quote: ... Total genomic DNA from each sample was extracted using the TIANamp Bacteria DNA isolation kit (DP302-02, Tiangen, Beijing, China). The quality of the extracted DNA was monitored on 1% agarose gels using electrophoresis and spectrophotometry (optical density at 260/280 nm ratio) ...
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bioRxiv - Microbiology 2020Quote: The genome DNA of infected soybean hairy-roots was extracted using New Plant Genome Extraction Kit (Cat: DP305, Tiangen biotech) and used for qPCR with ChamQ SYBR qPCR Master Mix (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... and the total RNA and nuclear RNA were extracted using RNAprep Pure Plant Plus Kit (Polysaccharides & Polyphenolics-rich, TIANGEN, DP441) according to the manufacturer’s instructions.
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bioRxiv - Pathology 2022Quote: ... Plant total RNA were extracted according to the instruction of the polysaccharide polyphenol plant total RNA extraction kit (Tiangen: DP441). The contaminating DNA was eliminated using RNase-free DNase and the first-strand cDNA was synthesized using Revert Aid First Strand cDNA Synthesis Kit (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... The lncRNA was reverse transcribed into cDNA using a lnRcute lncRNA First-Strand cDNA kit (4992908; Tiangen Biotech, Beijing, China) and quantified using a lnRcute lncRNA qPCR kit (4992886 ...
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bioRxiv - Plant Biology 2021Quote: ... Reverse transcription of miRNAs into cDNAs was carried out using the miRcute Plus miRNA First-Stand cDNA Synthesis Kit (Tiangen). Quantitative real-time PCR was performed using the 7500 Fast Real-Time PCR engine (Applied Biosystems ...
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bioRxiv - Plant Biology 2021Quote: ... and pUBQ10:MSD2ΔSP-mCherry. Mutagenesis of MSD2/Y68F was conducted with the Fast Site-Directed Mutagenesis Kit (cat. n. KM101) from TianGen. The constructs were introduced into plants by the floral dip method [21] ...
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bioRxiv - Plant Biology 2021Quote: The roots of 7 days old seedlings were collected to extract total RNA using RNA prep pure plant kit (TIANGEN). The first strand cDNA was synthesized from 1 μg of total RNA using the Hifair® III 1st Strand cDNA Synthesis SuperMix kit (YEASEN ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: PBMCs isolated from blood samples were extracted with total RNA using the Trizol method (total RNA Extraction Kit, DP431, Tiangen), and the purity NanoDrop2000 ...
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bioRxiv - Genomics 2020Quote: ... China) and first-strand cDNA was synthesized with 1µg RNA using a FastQuant RT Kit (with gDNase) (TIANGEN, Beijing, China). Gene-specific primers were designed using Primer-BLAST software (National Center for Biotechnology Information ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was extracted from the rat hippocampus after various treatments using an RNA-simple Total RNA Kit (TIANGEN, China) and then reverse transcribed using a ReverTra Ace qPCR RT Master Mix Kit (TOYOBO ...