Labshake search
Citations for TianGen Biotech :
501 - 548 of 548 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated from single third-instar larva of three strains (total 30 larvae of each strain) using the DNA/RNA/Protein Isolation Kit (TianGen, Beijing, China). The first-strand cDNA was synthesized with 1 μg of total RNA using a PrimeScript™ 1st Strand cDNA Synthesis Kit with gDNA Eraser (TaKaRa Biotechnology ...
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bioRxiv - Synthetic Biology 2021Quote: ... The incubated solution is then subjected to agarose gel electrophoresis and the correct band is extracted using a TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Synthetic Biology 2021Quote: ... The incubated solution is then subjected to agarose gel electrophoresis and the correct band is extracted using a TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Plant Biology 2020Quote: RNA was extracted according to the instructions of the RNA Isolation Kit for polyphenol- and polysaccharide-rich plants (Tiangen Biotech, Beijing, China). The reverse transcription primer was Oligo(dT)18 ...
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bioRxiv - Plant Biology 2020Quote: ... MG), 47 dpa(Breaker stage, BR) and 54 dpa(Red Ripe stage, RR) using the RNAprep pure Plant Kit (Tiangen, Beijing, China) based on the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA (1 µg) was used as a template for cDNA synthesis with a QuantScript RT Kit (KR103; Tiangen Biotech, Beijing, China). To determine mRNA expression levels ...
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bioRxiv - Plant Biology 2021Quote: ... Genomic DNA has been extracted from putative transformants one-month-old leaves grown on a kanamycin-containing medium using TianGen kits (TianGen BioTech, China). The quality of the extracted genomic DNA (250–350 ng/μl ...
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bioRxiv - Genetics 2021Quote: Genomic DNA was extracted from peripheral blood samples collected from all the participants using the blood DNA extraction kit (TianGen Biotech, China). We performed the whole genome sequencing on Illumina NovaSeq 6000 system (150 bp paired-end reads ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA was extracted from blood samples collected from the wing vein using a DNA extraction mini kit (Tiangen Biotech, Beijing, China). All sampled hens were killed by cervical dislocation immediately after oviposition and the abdominal cavity was opened ...
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bioRxiv - Bioengineering 2023Quote: Total RNAs were isolated from all samples with liquid nitrogen using the RNAprep Plant RNA kit (polysaccharides&polyphenolics-rich) (TIANGEN BIOTECH (BEIJING)CO. ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from 1×106 THP1 cells after various treatments (see above) by using an RNAprep pure cell/bacteria kit (Tiangen Biotech 4992235) according to the manufacturer’s instructions and was quantified using NanoDrop OneC Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: Experimental cells were subjected to total RNA isolation (using an RNA Simple Total RNA Kit, DP419, Tiangen Biotech Co., Ltd., Beijing, China). The first strand of cDNA was obtained by adding total RNA (1.5 µg ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA was isolated from the cecal contents using the magnetic-based soil and stool DNA extraction kit (TIANGEN®, Beijing, China). The concentration of the extracted DNA was determined using the Qubit Fluorometric Quantitation system (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... the DNA fragments were separated by 1.0 % agarose gel electrophoresis (1 × TAE buffer) and purified from the gel with the DNA Gel Extraction Kit (TianGen Biotech., Beijing, China), then they were sub-cloned into pCE2 TA/Blunt-Zero vector (Vazyme ...
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bioRxiv - Microbiology 2023Quote: CT DNA was extracted from the culture supernatant or cell lysates using the TIANamp Genomic DNA kit (Tiangen Biotech, Beijing, China; DP304). Quantitative PCR analysis targeting the CT cryptic plasmid was performed using TaqMan Gene Expression Master (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... and total mRNA was extracted using the RNA Prep Pure Plant Plus Kit according to the manufacturer’s instructions (Tiangen Biotech, Beijing, China). A strand-switching method and the cDNA-PCR Sequencing Kit (SQK-PCS109 ...
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bioRxiv - Zoology 2024Quote: ... The RT-qPCR reactions were carried out on the PikoReal 96 Real-Time PCR System (Thermo) using the SuperReal PreMix Color (SYBR Green) kit (Tiangen, Beijing, China). Three technical replicates were applied for each biological replicate ...
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bioRxiv - Genomics 2024Quote: ... total RNA of EPCs treated with NC or Tfeb-si1 were extracted using the RNAsimple Total RNA Kit (TIANGEN, cat. no. DP419). Library construction was performed using the SHERRY protocol as described previously [86] ...
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bioRxiv - Plant Biology 2024Quote: ... DNA was extracted from ‘Nagafu No.2’ (standard-type) and ‘Yanfu No.6’ (spur-type) leaves using a Super Plant Genomic DNA Kit (TIANGEN, DP360, China). Approximately 300 ng of DNA was treated with bisulfite using an EZ DNA Methylation-Gold Kit (Zymo Research ...
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bioRxiv - Microbiology 2024Quote: ... and brain tissues from diseased golden pompanos and infected cell cultures were extracted using a commercial RNA extraction kit for marine animals (Tiangen, Beijing, China). The concentration and purity of the extracted RNA were determined using Nanodrop 2000 (Thermo Scientific ...
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bioRxiv - Genetics 2024Quote: ... These two DNA fragments were ligated behind ubiquitin (Ubi) promoter in vector pWMB110 using the EasyGeno Assembly Cloning Kit (TianGen, Beijing, China). The two plasmids were introduced into stripe rust susceptible common wheat cultivars Fielder and Yannong 19 via Agrobacterium tumefaciens (strain GV3101 ...
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bioRxiv - Cancer Biology 2021Quote: Each of experimental cell lines was subject to its total RNAs isolated by employing an RNAsimple Kit (Tiangen Biotech CO. LTD, Beijing, China). Total RNAs (1 μg ...
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bioRxiv - Genetics 2022Quote: ... The CDS was cloned into the MCS of the modified pWMB110 vector under the ubiquitin promoter by using the EasyGeno Assembly Cloning kit (TianGen Biotech Co, Beijing). The recombinant plasmid was transformed into Agrobacterium strain C58C1 (Zoman Biotech Co ...
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bioRxiv - Pathology 2021Quote: ... Total RNA was extracted from mycelia of transformants cultured in V8 juice medium by using an RNA-simple Total RNA Kit (Tiangen Biotech, Beijing, China). PsFYVE1 specific primers (S5 Table ...
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bioRxiv - Plant Biology 2020Quote: ... Genomic DNA was extracted from the leaves of T3 transgenic plants (Table S8, lines 1954-1 and 1955-3) using a plant genomic DNA Extraction Kit (Tiangen Biotech, Beijing, China). About 20 μg of DNA was successfully digested with 5 U of EcoRV and incubated at 37 °C for 24 h ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from leaves of transgenic lines and wild-type plants treated with pathogens or hormones using the RNAprep pure plant kit (TIANGEN, DP441, Beijing, China). Then ...
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bioRxiv - Microbiology 2021Quote: The genomic DNA of the B3 strain was extracted using a Bacterial Genomic DNA Purification Kit (DP302-2, Tiangen Biotech Co., Beijing, China) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was extracted from mid-exponential cultures of strain HP1 (both the control and experimental groups) using the RNAprep Pure Bacteria Kit (Tiangen Biotech, Beijing, China) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... Cells harboring correct plasmid were cultured and pL4440-vit2 was extracted with the TIANprep Mini Plasmid Kit (Tiangen Biotech Co. Ltd., Beijing, China). Meanwhile ...
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bioRxiv - Cell Biology 2022Quote: ... The residual plasmid frame was then inserted by the synthesized combined fragment (GL-SV40pA-pTK-RF-bGHpA) using the EasyGeno Single Assembly Cloning kit (Tiangen Biotech, Beijing, China) to construct the dual-luciferase reporter plasmid (pGL-RF) ...
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bioRxiv - Microbiology 2022Quote: ... at which point 1 mL of cells were harvested and RNA samples were extracted using the RNAprep Pure Bacteria Kit (Tiangen Co., Beijing, China). Total cDNA was generated from 500 ng of total template RNA using the HiScript III All-in-one RT SuperMix Perfect for qPCR (Vazyme Co. ...
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bioRxiv - Biochemistry 2021Quote: Tea plant tissues or root materials were ground in liquid nitrogen into fine powders for total RNA extraction with an RNA extraction kit (Tiangen Biotech Co., Ltd.) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted from cell line K562 and liver tissues of HCC mice and healthy mice with the cell/tissue genomic DNA extraction kit (Tiangen, cat. no. DP304) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 200 mg 14-d-old seedlings grown under LD conditions using the RNAprep pure Plant Kit (DP432; Tiangen Biotech, Beijing, China). Sequencing libraries were prepared using the KAPA stranded mRNA-Seq Kit for the Illumina® Platform (KR0960-v.5.17 ...
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bioRxiv - Genomics 2024Quote: ... Total RNA was isolated from the blood of HU3095 (Supplementary Table 1) using an RNAprep Pure Tissue Kit (Cat# 4992236, TIANGEN Biotech, Beijing, China) according to the manufacturer’s instructions ...
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Integrative Omics reveals genetic basis and TaMYB7-A1’s function in wheat WUE and drought resiliencebioRxiv - Plant Biology 2024Quote: ... First-strand complementary DNA (cDNA) was synthesized from 2 μg of DNase I-treated total RNA using the FastKing RT kit (KR116, TIANGEN BIOTECH CO., LTD). RT-qPCR was performed using the ChamQ Universal SYBR qPCR Master Mix (Q711-03 ...
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bioRxiv - Plant Biology 2020Quote: ... We performed site-directed mutagenesis of K206 (K206R, AAG-AGG; K206Q, AAA-CAA) within the PET28a plasmid using the Fast Site-Directed Mutagenesis Kit (Tiangen, China, Catalog No. KM101). PCR was conducted using site-specific primers (Supplemental Table S17) ...
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bioRxiv - Genetics 2020Quote: ... Genomic DNA was isolated from positive samples by lysis with GS buffer containing proteinase K as described in the TIANamp Blood DNA Kit (TIANGEN Biotech Co. Ltd, Beijing) and stored at −20°C ...
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bioRxiv - Molecular Biology 2020Quote: RNA in vitro MCF-7 cell line and in vitro breast carcinoma tumor tissue were isolated using RNA prep pure cell/Bacteria kit and RNeasy reagents (TIANGEN BioTech Co.LTD, Beijing, China). Concentration and purity of RNA were detected by nucleic acid analyzing machine (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was isolated from positive samples by lysis with GS buffer containing proteinase K as described in the TIANamp Blood DNA Kit (TIANGEN Biotech Co. Ltd, Beijing) and stored at -20°C ...
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bioRxiv - Zoology 2023Quote: Genomic DNA of the strain E2 was extracted using a TIANamp Bacterial DNA Kit according to the manufacturer’s protocol (Tiangen Biotech Co., LTD, Beijing, China). The PacBio and Illumina Miseq sequencing of the strain E2 genome and bioinformatic analysis were performed by Guangdong Magigene Biotechnology Co. ...
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bioRxiv - Microbiology 2022Quote: ... genomic DNA of strains and related Pf prophage mutation strains were isolated using the TIANamp Bacteria DNA kit (Tiangen Biotech Co. Ltd, Beijing, China) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μg of purified RNA was reverse-transcribed in a 20 µL reaction using a FastQuant RT Super Mix Kit (TIANGEN, catalog no. KR106-02). Real-Time PCR was then performed by using Maxima SYBR Green/ROX kit (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from tissues of tea plant as well as Arabidopsis thaliana using an RNAprep Pure Plant Kit (Tiangen Biotech Co., Ltd, China) according to the manufacturer’s protocols ...
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High-resolution chromosome-level genome provides molecular insights into adaptive evolution in crabsbioRxiv - Genomics 2024Quote: ... cDNA synthesis was conducted using 500 ng of DNase-treated RNA and the Talent qPCR Premix (SYBR Green) kit (TIANGEN Biotech Co., Ltd., Beijing), in accordance with the manufacturer’s introductions ...
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bioRxiv - Cancer Biology 2022Quote: cDNA was synthesized using a miRcute plus miRNA cDNA first-strand synthesis kit (Cat. No. KR211) according to a standard protocol (Tiangen Biotech Co., Ltd., Beijing, China). RT–qPCR was performed via a miRcute plus miRNA qPCR kit (SYBR Green ...
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bioRxiv - Synthetic Biology 2022Quote: ... All cells in each well were harvested 3 days post-transfection and the genomic DNA was extracted by TIANamp Genomic DNA Kit (TIANGEN Biotech Co., Ltd., Beijing, China) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: Total RNA in planktonic and biofilm cells of PAO1 and MPAO1 were isolated using the Bacteria RNAprep pure kit (Tiangen Biotech Co. Ltd, Beijing, China) according to the manufacturer’s instructions ...