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Citations for TianGen Biotech :
451 - 500 of 548 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Total RNA of formalin-fixed paraffin-embedded (FFPE) explanted lungs from lung transplantation of two COVID-19 patients was extracted through RNAprep Pure FFPE Kit (TIANGEN), and used for the further verification of the two identified SARS-CoV-2-Encoded svRNAs ...
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bioRxiv - Genomics 2021Quote: ... Total DNA was acquired from 100mg fresh young leaves using a plant genomic DNA kit (Tiangen Biotech, Beijing, Co., Ltd.). Finally ...
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bioRxiv - Cancer Biology 2023Quote: ... total RNA from the cells plated on 96 well plates were first extracted and purified using the RNAprep Pure Micro Kit (TIANGEN). Reverse transcription was performed with HiScript III cDNA Synthesis Kit (Vazyme) ...
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bioRxiv - Plant Biology 2022Quote: ... gDNA buffer was added into total RNA to remove DNA impurity and then the purified RNA was used as a template to synthesized cDNA using a FastKing RT Kit (TIANGEN). The RT-qPCR assays were performed on qTOWER3 Real-Time PCR thermal cyclers (Analytik Jena ...
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bioRxiv - Plant Biology 2024Quote: ... T1 or higher generation embryos and regenerated plantlets respectively using the Plant Genomic DNA isolation Kit (TianGen Biotech., Beijing, China). For T0 and T1 resistant embryos ...
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bioRxiv - Zoology 2024Quote: The CRKP isolates were subjected to total genomic DNA extraction using the TIANamp Bacteria DNA Kit (Tiangen Biotech, Beijing, China) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... and plasmids were assembled by using HiFi DNA Assembly Cloning Kit and subsequently transformed into DH5α competent cells from TIANGEN. ZymoPure Midiprep Kit (Zymo Research ...
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bioRxiv - Plant Biology 2024Quote: The expression of DEGs identified by the transcriptomic analysis and the expression of the key genes involved in flavonoid/anthocyanin biosynthesis was validated by RT qPCR using a FastKing RT Kit from Tiangen Co ...
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bioRxiv - Biochemistry 2022Quote: Total RNAs were prepared from cells using the RNA Simple Total RNA Kit (DP419, Tiangen Biotech Co., Ltd., Beijing, China), and reverse transcription was performed using the Revert Aid First Strand cDNA Synthesis Kit from Thermo ...
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bioRxiv - Genetics 2022Quote: Genomic DNAs were collected from one small piece of ear tissues of cloned newborn piglets using TIANamp Genomic DNA Kit (TIANGEN). Then the genomic DNAs were used as a PCR template and PCR products were analyzed by agarose gel electrophoresis for identification of the cloned piglet genotypes ...
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bioRxiv - Molecular Biology 2023Quote: RBPs of interest were cloned into pcDNA3-3HA-hADAR2cd-E488Q13 to build pcDNA3-3HA-RBP-hADAR2cd-E488Q using Gibson assembly method46 (EasyGeno Single Assembly Cloning kit, Tiangen). PGK1 and CYC1 were acquired from yeast gDNA ...
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bioRxiv - Biophysics 2022Quote: ... the PCR amplified PAM-containing products were purified by VAHTS DNA Clean Beads (Vazyme) and further amplified by TIANSeq Fast DNA Library Prep Kit (TIANGEN) for Illumina Novaseq PE150 sequencing ...
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bioRxiv - Genomics 2024Quote: ... Leaves of plants grown at the E3 stage40 were collected and pooled for DNA extraction using the DNAsecure Plant Kit (TIANGEN). For Illumina short-reads sequencing ...
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bioRxiv - Plant Biology 2024Quote: ... the sequence encoding full-length ICE1 was cloned into pET28a using ClonExpress II One Step Cloning Kit (Vazyme) and transformed into Escherichia coli strain BL21 (DE3) (TIANGEN). The protein was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Genetics 2024Quote: ... the leaves of L20191212 and 4100 were collected and total RNA was extracted as described in the miRNeasy Mini Kit (Cat: 217004; TIANGEN). Consequently ...
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bioRxiv - Bioengineering 2024Quote: ... NECC organoids treated with 0.1 μM Quisinostat 2HCl or PBS for 48 h were isolated using the RNAprep Pure Micro Kit (Tiangen Biotech) and then sent for sequencing (Novogene) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was extracted from transfected HEK-293T from each subline (1×105) with the TIANamp genomic DNA Kit (DP304-02; Tiangen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Genome DNA from the collected cells of P2 was extracted under the instructions of the user’s manual for TIANamp Genomic DNA Kit (TIANGEN, DP304-02). PCR was later conducted to amplify mitochondrial COI gene fragment using the primer pair as follows ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA (gDNA) was isolated from adductor muscle tissues in parents and offspring using the TIANamp Marine Animals DNA Kit (TIANGEN, Beijing). Library preparation and high-throughput sequencing were conducted by Novogene (Beijing ...
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bioRxiv - Synthetic Biology 2020Quote: ... mRNA was extracted from the harvested cells according to the manufacturer’s instructions of a RNAprep Pure Cell/Bacteria Kit (Tiangen, Beijing, China). PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa ...
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bioRxiv - Plant Biology 2020Quote: ... The pCSGAPO1 vector (Cong et al., 2013) was digested with Bsa I and purified using a DNA purification kit (Tiangen, China). A ligation reaction (10 μl ...
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bioRxiv - Plant Biology 2021Quote: ... and the leaf samples were pooled for each group for DNA extraction using the TIANGEN DNA purification kit (TIANGEN, DP305-02). High-throughput sequencing and Bulked Sequence Analysis (BSA ...
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bioRxiv - Neuroscience 2021Quote: RNA was extracted from mouse brain lysates (mixture of 3∼4 mouse brains for each sample) using RNAprep pure Tissue Kit (Tiangen, DP431). cDNA was synthesized using HiScript II 1st Strand cDNA Synthesis Kit (Vazyme ...
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bioRxiv - Cell Biology 2021Quote: ... the first batch of cells (5×106) were collected 2 days after infection using the TIANamp genomic DNA kit (Tiangen, DP304), and the second batch of cells treated with puromycin were collected at 7d ...
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bioRxiv - Microbiology 2022Quote: The DNA of pure bacterial colonies was extracted by following the procedures described in the DNA extraction kit (TIANGEN Biotech, Beijing). The 16S rRNA gene was amplified using universal primers 27F (5’-AGAGTTTGATCMTGGCTCAG-3’ ...
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bioRxiv - Neuroscience 2022Quote: ... The strains were expanded and cultured for endotoxin-free plasmid extraction with the EndoFree Maxi Plasmid Kit V2 (DP120, Tiangen, China). The constructed plasmids were stored at −20 °C for the transfection of HEK293-T cells (49).
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from middle part of ear leaf at 12 DAI with the RNA extraction Kit (DP411, TIANGEN, China). RNA purity ...
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bioRxiv - Cancer Biology 2023Quote: The total RNAs were extracted from experimental cells when they reached 80% confluency using an RNA extraction kit (TIANGEN, Beijing, China) and then reversely transcribed into single-stranded cDNAs by a Reverse-Transcription Kit (Promega ...
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bioRxiv - Microbiology 2024Quote: ... viral RNA was extracted from mock- or ALSV-infected cell-culture supernatants using a viral RNA extraction kit (TIANGEN, Cat# DP315), and transcribed into cDNA using the cDNA Synthesis SuperMix (TransGen ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from less than 100 mg of 10-d-old seedlings that were grown vertically on half-strength MS plates or treated with 50 μM AA or an equal volume of DMSO for 4 h in liquid MS medium using RNAprep Pure Plant Plus Kit (TIANGEN, DP441). First-strand cDNA was synthesized using a HiScript® II cDNA Synthesis Kit (Vazyme R212-02 ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from leaf and root tissues of the natural population and transgenic rapeseed using RNAsimple Kit (DP419, TIANGEN, China). The RNA was sequenced using Illumina 2500 platform (Illumina Inc ...
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bioRxiv - Plant Biology 2024Quote: Total RNAs was extracted from 100 mg cotton seedling samples with different treatments by grinding with Plant Total RNA Purification Kit (TIANGEN, China). RNA was then treated with DNase using the DNA-free kit following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: Total RNA was extracted from blood using TRIzol reagent in an RNAprep Pure Tissue Kit (Cat# 4992236, TIANGEN Biotech, Beijing, China). After the RNA purity and concentration were determined with Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... First-strand cDNA was synthesized from 2 μg of DNase I-treated total RNA using the FastKing RT kit (TIANGEN, KR116). qRT-PCR was performed using the ChamQ Universal SYBR qPCR Master Mix (Vazyme ...
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bioRxiv - Genomics 2024Quote: ... A paired-end library with an insert size of 350 kb was constructed using the TIANSeq Fast DNA Library Kit (Cat. no: 4992261; Tiangen, China) and then sequenced using an Illumina NovaSeq6000 sequenator (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from cultured cells by using TRIZOL reagent and 500 ng to 2 μg RNA was reversely transcribed into first-strand cDNA by FastKing RT Kit (Tiangen, KR116). qPCR analysis was performed with Mastercycler Realplex2 (Eppendorf ...
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bioRxiv - Genomics 2022Quote: ... on an agarose gel and were subsequently extracted using a Universal DNA purification Kit (Catalog No. DP214-02, TIANGEN, Beijing, China) and then used as a template to synthesize dsRNA with a MEGAscript RNAi Kit (Catalog No ...
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bioRxiv - Plant Biology 2022Quote: ... Reverse transcription and quantitative real-time PCR assay were performed in a Bio-Rad iQ5 real-time system using a Quant One Step qRT-PCR (SYBR Green I) Kit (Tiangen Biotech). Transcript expression levels of FmASP were normalized to that of the reference gene ACTIN and calculated using the 2-ΔΔCt method (Livak and Schmittgen ...
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bioRxiv - Plant Biology 2022Quote: ... the young leaves of eight to ten seedlings were sampled and pooled for genomic DNA extraction using the Plant Genomic DNA Extraction Kit (DP205, TianGen, China) following the instructions provided by the supplier.
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bioRxiv - Immunology 2023Quote: ... hCD36 mice below refer to homozygous hCD36 mice) and wild-type (WT) C57BL/6J were harvested for extracting total RNA using RNAprep Pure Cell / Bacteria Kit (TIANGEN, DP430). The mRNA expression of hCD36 and mCD36 was determined using GAPDH as the internal control ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmid was isolated from a single transformant in LB medium with kanamycin (50 μg/mL) using DNA purification kits purchased from Tiangen (China) and verified by DNA sequencing(RuiBiotech ...
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bioRxiv - Microbiology 2024Quote: Total RNA extracted from ‘Nipponbare’ leaves was incubated at 37°C for 30 min with 2 U RNase R (Geneseed) and then purified using an RNA clean kit (DP412; Tiangen, China). Nucleoplasmic separation experiments and RNA-FISH in rice cells were performed as previously described (Gao et al. ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from plant materials using an RNAprep Pure Plant Kit with on-column DNase digestion (Tiangen Biotech, China) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Both DRAMmut-ABE and DRAMmut-CBE related vectors were obtained by introducing the corresponding key amino acid mutations using Fast Site-Directed Mutagenesis Kit (TIANGEN Biotech). The primer sequences used are listed in Supplementary Table 4.
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bioRxiv - Microbiology 2024Quote: ... The recombinant vector PiggyBac [A3-EGFP-SV40+A3-Neo-SV40+IE2-NbSPN14-SV40] was extracted from the DH5α cells with the TIANpure Mini Plasmid Kit (TIANGEN, China). BmE cells were transfected with 2 μg of this recombinant plasmid and A3 helper plasmid using X-tremeGENE HP DNA Transfection Reagent (Roche ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and stem samples from the four female plants and male catkin samples from each four lineages using a Polysaccharide Polyphenol Plant Total RNA Extraction Kit (Tiangen, China). The integrity of RNA was assessed using the Fragment Analyzer 5400 (Agilent Technologies ...
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bioRxiv - Plant Biology 2020Quote: Total RNA samples extracted from M82 and yft1 pericarp (47 dpa, BR stage) using the RNAprep pure Plant Kit (Tiangen, Beijing, China) were used as a templates in rapid amplification of cDNA ends (RACE ...
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bioRxiv - Plant Biology 2020Quote: ... AAA-CAA) within the PGEX-6P-1 plasmid was performed using the fast Site-Directed Mutagenesis Kit (Tiangen, China, Catalog No. KM101). PCR was conducted using site-specific primers (Supplemental Table S17) ...
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bioRxiv - Microbiology 2021Quote: ... of strain TC967 was extracted from 4-day-old mycelia following the protocol described in the handbook of the Plant Genomic DNA Kit (Tiangen, Beijing, China). The gDNA was sequenced by Illumina HisSeq 2500 sequencing (Illumina ...
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bioRxiv - Genetics 2022Quote: ... First-strand cDNA synthesis from the total RNA was performed by using the FastKing RT kit (with gDNase) (TianGen Biotech Co, Beijing). The expression analyses were performed using the primer set (F-GGACTTCCCTTGGATCCTGC and R-ACCGACAATCATGTCCGCAT) ...