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Citations for System Biosciences :
101 - 150 of 156 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... The expression vector coding GCaMP6s was introduced into MDCK cells with Super PiggyBac transposase (System Bioscience). After a few days ...
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bioRxiv - Cell Biology 2023Quote: ... The tag donor was also introduced as a minicircle generated with the MC-Easy Minicircle DNA Production Kit according to manufacturer’s instructions (System Biosciences, MN920A-1). Both solutions were combined ...
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bioRxiv - Cell Biology 2023Quote: ... The tag donor was also introduced as a minicircle generated with the MC-Easy Minicircle DNA Production Kit according to manufacturer’s instructions (System Biosciences, MN920A-1). Both solutions were combined ...
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bioRxiv - Cell Biology 2021Quote: ... The vectors were transduced into HeLa cells using the PiggyBac transposon system (System Biosciences, Palo Alto, CA), and the cells were selected for 2 weeks with 2 μg/ml of puromycin ...
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bioRxiv - Cell Biology 2021Quote: ... MNT1 cells were transfected with Pb-TRE3G-SpCas9-IRES-Blast and piggyBac transposase (PB210PA-1, System Biosciences) in 1:3 ratio using lipofectamine (Life Technologies ...
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bioRxiv - Genomics 2021Quote: ... was stably introduced in HEK293T cells using the piggy-transposase system (System Biosciences, cat. no. PB210PA-1) according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2022Quote: ... freshly dissociated brain cells were first transduced with lentivirus delivering human SNIP1 transgene (System Biosciences CD823A-1) in ultra-low attachment 6-well plates ...
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bioRxiv - Genomics 2022Quote: THP-1 cells were transduced in triplicates using the TransDux MAX transfection reagent (System Biosciences, LV860A-1) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2020Quote: ... All other 293T-GFP responder cell lines were constructed by lentiviral transduction (pCDH vector backbone, System Biosciences). All transient expression plasmids were cloned into the pCS2+ vector using standard restriction cloning ...
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bioRxiv - Immunology 2024Quote: ... The HEK293TN-hACE2 cell line was generated by lentiviral transduction of HEK293TN (System Bioscience, Cat#LV900A-1) cells as described in Notarbartolo S ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lines were immediately cryopreserved and maintained in selection medium containing 60 μg/mL cumate (System Biosciences) for at least one passage prior to assay.
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bioRxiv - Synthetic Biology 2020Quote: ... Exo-Check Array: A549 protein markers were characterised using a commercially sourced Exo-Check Exosome Antibody Array kit (#EXORAY200A-4, System Biosciences, CA, USA). A549 EVs (100 μg ...
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bioRxiv - Physiology 2023Quote: Isolated Evs were processed for total RNA isolation using the SeraMir Exosome RNA purification kit (Cat #RA808A-1, System Biosciences, Palo Alto, CA), according to the manufacturer’s instructions provided with the kit ...
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bioRxiv - Cell Biology 2021Quote: ... Cas9-MNT1 cells were transfected with Pb459-U6-sgRNA-EF1a-PuroR and piggyBac transposase (PB210PA-1, System Biosciences) in a 1:3 ratio using lipofectamine (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviruses containing the luciferase expression cassette were produced in 293 TN cells (System Bioscience, catalog number: LV900A-1) by transient transfection with the PSMPUW-Hygro lentiviral vector carrying the luciferase gene and the ViraSafe™ Lentiviral Packaging System (Cell Biolabs ...
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bioRxiv - Developmental Biology 2022Quote: ... The amplified coding sequences followed by p2a-tdTomato were cloned downstream of the endothelial specific fli1a promotor into a Tol2 enabled vector using the Cold Fusion Cloning kit (System Biosciences, Cat# MC010B-1).
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bioRxiv - Molecular Biology 2019Quote: The identification of protein markers on the isolated exosomes was undertaken using the commercially available Exo-Check Exosome Ab Array kit (System Biosciences, Palo Alto, CA), as described by the manufacturer ...
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bioRxiv - Cell Biology 2019Quote: ... the collected exosomes were further purified with anti-CD9 antibody-coated magnetic beads (CD9 Exo-Flow Exosome Purification Kit, System Biosciences, Cat. EXOTC10A-1) as in (Rim and Kim ...
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bioRxiv - Cancer Biology 2021Quote: ... The transfer vector was then packaged into a lentivirus using the pPACKH1 HIV third generation lentiviral expression system kit and PureFection reagent (both from System Biosciences, Palo Alto, CA) into HEK293-T cells (ATCC ...
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bioRxiv - Neuroscience 2022Quote: ... 0N4R tau was replaced with 2N4R tau after inserting 2N4R tau between BamHI and SalI restriction sites using a cold fusion cloning kit (System Biosciences, Catalog # MC100B-1). A Q5 Site-Directed Mutagenesis Kit (New England BioLabs ...
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bioRxiv - Cancer Biology 2023Quote: EV pellets were diluted and 45 µL was examined with the ExoELISA-ULTRA CD63 Kit according to the manufacturer’s instructions (System Biosciences, Palo Alto, CA, USA). The sample absorbance minus the blank wells was used to determine concentrations based on the standard curve included in the kit.
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bioRxiv - Biochemistry 2021Quote: ... Plasmids for stable cell line construction were generated by Gibson cloning into pCDH-EF1-CymR-T2A-Neo (System Bioscience). Sequences of all plasmids were confirmed by Sanger sequencing.
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bioRxiv - Molecular Biology 2020Quote: The Jurkat cell transductions were done with multiple viral titers using TransDux™ MAX Lentivirus Transduction Reagent (System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Stable cell lines were generated by cotransfection with the Super PiggyBac Transposase Expression Vector (cat# PB210PA-1, System Biosciences) followed by selection via addition of 5 μg/mL puromycin (cat# ant-pr-1 ...
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bioRxiv - Bioengineering 2023Quote: Stable HeLa-Fucci cell line was generated using the Super PiggyBac Transposase Expression Vector system (System Biosciences, PB210PA-1) together with tFucci(CA)5 plasmid29 (Addgene #153521) ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmids for stable cell line construction were generated by Gibson cloning into pCDH-EF1-CymR-T2A-Neo (System Bioscience) vector ...
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bioRxiv - Biophysics 2023Quote: ... Cells were transfected with 1500ng of HaloTag-NLS or HaloTag-H2b + 1000ng of piggyBac Transposase (System Biosciences, PB210PA-1) using Lipofectamine2000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: Exosomes were purified from cultured medium supplemented with exosome-depleted FBS using the exosome purification kit following manufacturer’s instructions (ExoQuick™, System Bioscience, Mountain View, CA, USA).
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bioRxiv - Molecular Biology 2020Quote: ... we replaced the KRAB and dCas9 cassettes from the pHR-SFFV-KRAB-dCas9-mCherry vector with the HA-tag-MEOX1 cassette using Cold-fusion cloning kit (SBI System BioSciences, Palo Alto, CA, USA) following the instruction provided by the manufacturer ...
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bioRxiv - Bioengineering 2022Quote: ... EVs were also quantified based on the amount of total immunoreactive CD63 within isolated EV samples using the ExoELISA-ULTRA Complete Kit (System Biosciences, EXEL-ULTRA-CD63-1) per the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... with 250 μg protein equivalent of AFSC-EVs stained using 2 μL of ExoGlow™-Vivo (Near IR) EV Labeling Kit (System Biosciences, Palo Alto, CA).
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bioRxiv - Cancer Biology 2019Quote: Cells were cultured in DMEM/1640 supplemented with 10% exosome-depleted FBS (EXO-FBS-250A-1, SBI System Biosciences, USA) using 10×10 cm2 disks (1×106 cells per disk ...
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bioRxiv - Genetics 2019Quote: To produce lentiviral cell lines containing pGreenFire1™ Pathway Reporter lentivector (Cat#TR010PA-N and Cat#TR000PA-1, System Bioscience) expressing the MUC5B promoter region and controls ...
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bioRxiv - Cancer Biology 2024Quote: ... stable HEK293-F cell lines for each cytokine were prepared through cloning into the expression cassette of PiggyBac (System Biosciences) transposon vector ...
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bioRxiv - Cell Biology 2020Quote: ... with the respective lamin containing or empty (Mock) vectors into HEK 293-TN cells using PureFection nanotechnology-based transfection reagent (System Biosciences). The medium was exchanged the next day and replaced by antibiotics-free DMEM containing 10% FCS ...
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bioRxiv - Cell Biology 2021Quote: TYR-G1-G4 knockout cell lines were transfected with the Pb-TRE3G-TYR-HygR plasmid and piggyBac transposase (PB210PA-1, System Biosciences) in a 1:3 ratio using lipofectamine (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... NK92 CD56-KO cells were transduced with pBabe-puro-NCAM mApple viral particles in the presence of MAX Enhancer and TransDux (System Biosciences). Within 48 hours cells were sorted for CD56 and mApple and cells were expanded and maintained under antibiotic selection with regular phenotypic validation.
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bioRxiv - Microbiology 2020Quote: The 3’UTR reporter plasmids were co-transfected into HEK293T cells using PEI max together with the indicated pCDH-EF1-MCS expressing plasmids (System Biosciences) containing a cloned pre-miRNA gene of interest ...
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bioRxiv - Immunology 2022Quote: ... encoding HLA-A*02:01 or HLA-A*03:01 by spinoculation with ultracentrifuge concentrated viral particles produced from 293T-HEK-TN cells (System biosciences), followed by sorting of cells stained with anti-human HLA-A ...
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bioRxiv - Developmental Biology 2019Quote: We acquired 6 human induced pluripotent stem cell (iPSC) lines (Sojd3, Hoik1, Kucg2, Wibj2 from the HipSci resource54; h409b2 from the RIKEN BRC cell bank17; Sc102a1 from System Biosciences), one human ES cell line (H9 ...
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bioRxiv - Bioengineering 2023Quote: ... The in vivo T cell activation reporter was constructed by cloning a sequence containing firefly luciferase into the pGreenFire1-NF-κB lentiviral vector (System Biosciences) under the NF-κB responsive promoter ...
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bioRxiv - Bioengineering 2023Quote: ... were purchased from the Riken Brain Science Institute (Japan) and used to generate lentivirus particles by co-transfecting HEK 293TN cells (System Biosciences) with packaging (psPAX2 ...
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bioRxiv - Cell Biology 2024Quote: Stable cell lines expressing GFP-tagged cytoplasmic tail chimeras under a cumate-inducible promoter were generated by PiggyBac transposition (System Biosciences). Wild-type ...
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bioRxiv - Cell Biology 2020Quote: ... purification of EVs was performed in plasma by UC-I (bellow) and in conditioned medium according to one of the following protocols (Fig.1a):-(Exoquick) ExoQuick® commercial kit (System Biosciences EXOTC10A-1, Palo Alto, CA), following manufacturer’s instructions;
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bioRxiv - Molecular Biology 2021Quote: ... Frozen primary human T cell pellets were thawed and then stimulated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Stemcell) for 48 hours and then transduced with various CAR viruses using TransDux™ MAX Lentivirus Transduction Reagent (System Biosciences) as described above ...
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bioRxiv - Cancer Biology 2022Quote: The infection of HuCCT1 cells with pGreenFire 2.0 TCF/LEF reporter virus (pGF2-TCF/LEF-rFluc-T2A-GFP-mPGK-Puro) (SBI System Biosciences, TR413VA-P) was performed using SureENTRY Transduction Reagent ...
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bioRxiv - Cell Biology 2024Quote: ... Extracellular vesicles were isolated from 10 mL of cell culture supernatant using the ExoQuick-TC® Exosome Precipitation Solution (cat# EXOTC10A-1; System Biosciences) according to the manufacturer’s instructions with a few modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... in which A375 Cas9 cells were co-transfected with the rescue plasmids as well as the Super PiggyBac Transposase Expression Vector (System Bioscience Inc, PB200PA) at a 4:1 ratio utilising Lipofectamine™ LTX reagents (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: Bare 293T cells were first co-transfected using PEI47 with the H2B-mTagBFP2 vector and Super PiggyBac Transposase-expressing vector (System Biosciences Inc. # PB210PA-1) via polyethylenimine (Sigma #408727-100mL ...
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bioRxiv - Cancer Biology 2020Quote: ... MDA-MB-231 and MDA-MB-231-Luc+ cells were transduced with lentivirus vector expressing a GFP or RFP tag fused to the membrane-associated tetraspanin CD63 (System Biosciences, Palo Alto, CA). Breast cancer cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM ...