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Citations for System Biosciences :
51 - 100 of 156 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... ExoQuick-TC ULTRA (for cell culture medium) (System Biosciences) was used according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Virus-containing supernatants were either collected or concentrated by mixing with quarter volumes of 5 × PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, United States), followed by centrifugation according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... these HEKs were genetically-modified reporter cells (NF-κB/293/GFP-Luc™ Transcriptional Reporter Cell Line; System Biosciences, catalogue number TR860A-I), but reporter activity is not relevant here.
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bioRxiv - Genetics 2019Quote: PCR amplification was performed according to the PrecisionX Multiplex gRNA Cloning Kit (System Bioscience). BbsI-linearized piggyBac-U6 backbone was mixed with purified PCE product in a vector:insert 2:1 ratio and was directly used for transformation of One Shot MAX Efficiency DH5α-T1 Competent Cells (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... and the rcccDNA was isolated using the MC-Easy minicircle production kit (System Biosciences) according to the manufacturer’s recommendations.
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bioRxiv - Immunology 2019Quote: ... The sgRNA transcripts were generated by IVT synthesis kit (System Biosciences, Palo Alto, CA). Quality of sgRNA transcripts was analyzed by NanoDrop (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: EV RNA was isolated with a SeraMir Exosome RNA Column Purification Kit (System Biosciences) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: Lentivirus was produced in 293TN cells (System Bioscience, LV900A-1) expanded in DMEM with 10% FBS as previously described (Beronja et al. ...
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bioRxiv - Immunology 2021Quote: ... 8×106 293TN Producer cells (System Biosciences, Cat # LV900A-1) were plated in T150 flasks overnight ...
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bioRxiv - Cell Biology 2024Quote: ... pseudoviral particles were produced using 293TN cells (System Biosciences, SBI). The 293TN cells were transfected with the lentiviral plasmid of interest as well as lentiviral packaging and envelope plasmids (psPAX and pMD2.G ...
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bioRxiv - Genomics 2023Quote: 16 million K562 cells (8 million K562-VP64 cells and 8 million K562-VPR cells) were transfected with the gRNA library and the piggyBac transposase (System Biosciences, Cat. No. PB210PA-1) at a 20:1 molar ratio of library:transposase using a Lonza 4D nucleofector and the Lonza nucleofector protocol for K562 cells ...
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bioRxiv - Cell Biology 2022Quote: ... Exosomes were labeled with ExoGlow™-Vivo EV Labeling Kit (System Biosciences, Palo Alto, CA). Labeled exosomes or PBS containing exosomes were injected via tail vein into 17-18-month-old C57BL/6 mice ...
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bioRxiv - Developmental Biology 2019Quote: ... Exosomes were then collected following the manufacturer’s instructions for the ExoQuick™kit (System Biosciences). For Western blot analysis ...
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bioRxiv - Microbiology 2020Quote: ... Fusion was performed with the Cold Fusion Cloning Kit (SBI System Biosciences, Cat. #MC010B-1).
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bioRxiv - Cell Biology 2021Quote: Inducible Cas9 iPSCs were generated using an AAVS1 TALE-Nuclease KIT (GE60xA-1; System Biosciences) and an iCas9 HR donor plasmid containing a TRE3G promoter-driven spCas9 with a C-terminal MYC NLS followed by a CMV promoter-driven Tet-On-3G and a neomycin resistance adapted from Ihry et al ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid was then packaged into viral particles using the pPACKH1 lentivector Packaging Kit (System Bioscience) in HEK293T cells ...
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bioRxiv - Bioengineering 2023Quote: ... Then MC DNA was produced and purified by using an MC-EsayTM Kit (System Biosciences), without the optional dNTP removal step.
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bioRxiv - Developmental Biology 2023Quote: ... or goat anti-rabbit (1:20,000; EXOAB-KIT-1; System Biosciences, Palo Alto, CA, USA). Protein signals were detected using the SuperSignal West Femto Chemiluminescent kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... exosomes from ASCs were labeled fluorescent red using an ExoGlow-Protein EV Labeling Kit (System Biosciences) and then incubated with precipitation solution at 4°C overnight ...
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bioRxiv - Neuroscience 2019Quote: ... miRNA was converted to cDNA using the QuantiMiR reverse transcription kit (System Biosciences, Mountain View, CA). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... or by labeling RNA cargos by using the ExoGlow-RNATM EV Labeling Kit (System Biosciences, Inc.) following the manufacturers’ recommendations ...
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bioRxiv - Zoology 2023Quote: ... EVs were isolated from the resulting supernatant using the ExoQuick Exosome Isolation kit from System Biosciences following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: Western blotting was performed using ExoA Antibody Kit according to the manufacturer’s protocol (System Biosciences, USA). Immunoblots were cut prior to primary antibody hybridization ...
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bioRxiv - Cell Biology 2021Quote: ... cells were cultured in EV-depleted FBS (System Biosciences, Palo Alto, CA). Plates (14 ...
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bioRxiv - Synthetic Biology 2023Quote: ... HEK293FT cells were transfected with the vector and transposon plasmid (System Biosciences). Transfected Cells were incubated for 72 hours before selection with media containing 2 ug/mL Puromycin (Invivogen ant-pr-1) ...
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bioRxiv - Neuroscience 2022Quote: NDE were isolated from total EVs of plasma using the Basic Exo-Flow Capture Kit (System Biosciences) as described (Winston et al. ...
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bioRxiv - Bioengineering 2023Quote: ... OMVs in the total supernatant fraction were extracted using the ExoBacteria™ OMV Isolation Kit (System Biosciences) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... and anti-rabbit HRP-linked secondary antibody (EXOAB-KIT-1) were purchased from System Biosciences (CA, USA). Anti-beta-actin antibody (sc-47778) ...
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bioRxiv - Microbiology 2021Quote: ... tHF cells stably transduced with the IFN-responsive pGreenFire-ISRE lentivector (System Biosciences) were further stably transduced with the lentiCRISPRv2 vector ...
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bioRxiv - Cancer Biology 2020Quote: SW480 CRC cells were transduced with lentivirus carrying pCDH vector from System Biosciences: empty vector (Mock ...
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bioRxiv - Developmental Biology 2020Quote: ... ESC were washed twice with PBS and incubated with AP Staining kit™ (Cat # AP100R-1, System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... and the EVs were isolated with a commercially available precipitation kit (ExoQuick, System Biosciences, Palo Alto, CA, US) following the protocol provided by the vendor ...
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bioRxiv - Cell Biology 2021Quote: ... A lentiviral plasmid containing guide RNA sequences was transfected into 293TN cells (System Biosciences) using Lipofectamine 2000 transfection reagent ...
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bioRxiv - Cancer Biology 2019Quote: ... Lentivirus was packaged in HEK293T cells (ATCC) and purified using PEG-it (System Biosciences).
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bioRxiv - Biochemistry 2021Quote: The NF-κB/Jurkat/GFPTM Transcriptional Reporter cell line was obtained from System Biosciences, and was cultured in RPMI-1640 media supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... and HEK 293TN (LV900A-1) cells were purchased from System Biosciences (Palo Alto, USA). Mouse mesothelial cells (MCs ...
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bioRxiv - Genetics 2023Quote: ... cells were transfected with 0.72 μg of piggyBac transposase plasmid (System Biosciences PB210PA-1) and 1.78 μg of pCX4.1 plasmid using Lipofectamine 3000 transfection reagent ...
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bioRxiv - Bioengineering 2021Quote: ... Quantification of EV release was accomplished using an ExoELISA-ULTRA Complete Kit (CD63 Detection; System Biosciences, Mountain View, CA). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... The cDNA was subsequently cloned into the pLVX tight puro vector using the Cold FusionTM cloning kit (System Biosciences), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... was added to a tube along with purefection reagent and pPackF1 lentiviral packaging kit plasmids (System Biosciences, # LV100A-1) and either PCF1 (empty vector control) ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... Quantification of successfully integrated piggyBac transposons was performed using a piggyBac qPCR copy number kit (System Biosciences, PBC100A-1) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... DLD-1 cells were co-transfected with a PiggyBac transposase vector (System Biosciences, PB210PA-1) and a PiggyBac-ABA-CRISPRi vector (25 ...
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bioRxiv - Microbiology 2021Quote: ... Vero E6 and Huh7.5 cells with PiggyBAC/ACE2 and integrase-encoding plasmids (System Biosciences, Inc.) using Lipofectamine 3000 ...
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bioRxiv - Biochemistry 2019Quote: ... cells were seeded at ~10% confluency in exosome-depleted medium (System Biosciences, Palo Alto, CA) in 150mm CellBIND tissue culture dishes (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... 1 million cells were co-transfected with 1 µg ePiggyBac transposase expression plasmid (System Biosciences) and 1 µg each epB-MCP-mScarletI ...
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bioRxiv - Bioengineering 2023Quote: HEK293T cells stably expressing mRNA constructs were generated using the ϕC31 integrase system (System Biosciences) according to the manufacturers protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified viral particles were transduced into MIN6 cells using TransDux MAX (LV860A-1; System Biosciences) and cultured for 48-72 hours in complete DMEM (11965-092 ...
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from the EVs using the Exoquick RNA column purification kit (EQ808A-1, System Biosciences, Palo Alto, CA, USA). Purified RNA was quantified using a Bioanalyzer and the Agilent RNA 6000 Pico kit (5067-1513 ...
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bioRxiv - Neuroscience 2024Quote: Total RNA isolation kits were employed to isolate RNA from cultured iMicroglia (n=4/group; System Bioscience, Palo Alto, CA, USA) and the mouse hippocampal tissues (n=5-6/group ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... cells were transfected with 0.2 μg of Super PiggyBac Transposase Expression Vector (System Biosciences, PB210PA-1) and 0.2 ...