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Citations for System Biosciences :
1 - 50 of 156 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... and used to transduce pre-activated primary human CD4+ T cells in combination with Transdux (System Biosciences). GFP+ cells were FACS sorted 48h later ...
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bioRxiv - Cell Biology 2022Quote: ... one day before the transfection of the expression construct of mCherry-proliferating-cell nuclear antigen (PCNA) or EGFP-ATM based on PB533A-2 (System Biosciences) with a PiggyBac transposon expression vector (PB210PA-1 ...
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bioRxiv - Molecular Biology 2021Quote: The primary human T cell transductions were done with multiple viral titers using TransDux™ MAX Lentivirus Transduction Reagent (System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: The mouse H3f3a sequence (encoding the histone H3.3A isoform) upstream an IRES and nuclear GFP sequences were cloned into the EcoRI and NotI sites of EEV600A-1 (System Biosciences) to generate the EEV expressing the WT histone H3.3A (supplementary Fig ...
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bioRxiv - Neuroscience 2021Quote: ... The number of CD63+ EVs collected in the EV-enriched fractions were estimated by ELISA (System Biosciences). The ELISA standards provided in the kit are calibrated by NTA to measure the number of exosomes and establish a standard curve based on exosome abundance ...
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bioRxiv - Microbiology 2021Quote: ... EBV-negative Akata(-) cells were treated with purified exosomes in RPMI1640 supplemented with 5% exosome-depleted FBS (System Biosciences, Palo Alto, CA, USA). After 6 h ...
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bioRxiv - Genetics 2023Quote: ... 6 X 105 cells were co-transfected with 5 µg of gRNA-containing piggyBac vector and 1 µg of piggyBac transposase plasmid (System Biosciences PB210PA-1) using Lipofectamine 3000 Transfection Reagent ...
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bioRxiv - Molecular Biology 2021Quote: ... with 1/5 final dilution (System Biosciences, LV810A-1), and the mixture was incubated at 4 °C for 6 hrs ...
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bioRxiv - Developmental Biology 2020Quote: ... 4 mL 5× PEG reagent (System Biosciences, LV825A-1) was added and mixed by pipetting ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Virus was concentrated using 5× PEG- it™ (System Biosciences) as per manufacturer’s protocol and virus pellet obtained was aliquoted in opti-MEM and stored at -80°C ...
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bioRxiv - Cell Biology 2023Quote: ... and 5 ng μL-1 Cas9 mRNA (System Biosciences, Palo Alto, CA) in 10 mM Tris-HCl pH 8.0 and 0.1 mM EDTA were used for injection into the pronucleus of fertilized mouse zygotes ...
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bioRxiv - Genetics 2019Quote: ... matched with Zfr knockdown: 5’-AACCGATGTACTTCCCGTTAAT) were cloned into the pGreenPuro backbone from System Biosciences. This construct was used to produce a 6-well of lentivirus according to standard protocols in 293T cells using the third-generation system and concentrated 1:100 with lenti-X (Clontec) ...
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bioRxiv - Physiology 2023Quote: ... and 5 µL of de-fibrination reagent (Cat #TMEXO-1, System Biosciences, Palo Alto, CA) was added to the plasma to remove clotting factors prior to the isolation of EVs ...
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bioRxiv - Developmental Biology 2022Quote: ... The gRNA (5 pg/embryo) and mRNA encoding Cas9 protein (150 pg/embryo, System Biosciences) were injected into one-cell stage embryos ...
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bioRxiv - Genetics 2021Quote: ... An in vitro transcribed sgRNA targeting Slc16a11 (5’-AGTCCTAACCTCGCTTGGCT-3’) and hspCas9 mRNA (CAS500A-1, System Biosciences) were microinjected into C57BL/6J mouse zygotes by the Genome Modification Facility at Harvard University ...
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bioRxiv - Microbiology 2021Quote: ... media were changed to DMEM supplemented with 5% exosome-depleted FBS (System Biosciences, Palo Alto, CA, USA). After 48 h ...
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bioRxiv - Microbiology 2022Quote: ... using the Multiplex gRNA kit (System Biosciences) according to the manufacturer protocol ...
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bioRxiv - Molecular Biology 2023Quote: HEK293T cells (System Biosciences) and mouse embryonic fibroblasts (MEFs ...
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bioRxiv - Neuroscience 2022Quote: ... 500 μL of human plasma were incubated with 5 μL purified thrombin (System Biosciences, Inc.; Catalog # TMEXO-1) at room temperature for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Cold Fusion kit (System Biosciences Inc) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... EVs were isolated from tissue culture media using a commercial kit containing a polyethylene glycol (PEG) solution (ExoQuick-TC ULTRA kit, EQULTRA-20TC-1; System Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... 293-TN cells (System Biosciences) were co-transfected with lentiviral packaging plasmid and envelope plasmid (psPAX2 and pMD2.G ...
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bioRxiv - Bioengineering 2023Quote: ... and the pPACKH1 HIV Lentivector Packaging Kit (System Biosciences) was used to produce lentivirus particles ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was blocked in freshly prepared 5% BSA diluted in TBS before being immunoblotted with specific primary antibodies (CD81; #EXOAB-CD81A-1 System Biosciences, GM130 ...
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bioRxiv - Molecular Biology 2019Quote: HEK293TN cells (System Biosciences, LV900A-1) and HeLA cells (American Tissue Culture Collection ...
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bioRxiv - Cell Biology 2022Quote: ... 293-TN cells (System Biosciences, SBI) were co-transfected with the lentiviral plasmid and lentiviral helper plasmids (psPAX2 and pMD2.G ...
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bioRxiv - Cancer Biology 2022Quote: ... 293-TN cells (System Biosciences, SBI) at 70-80% confluency were co-transfected with the lentiviral plasmid and lentiviral helper plasmids (psPAX2 ...
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bioRxiv - Cell Biology 2019Quote: ... 293-TN cells (System Biosciences, SBI) were co-transfected with the lentiviral-containing ...
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bioRxiv - Molecular Biology 2019Quote: HEK293TN cells (System Biosciences, LV900A-1) were cultured using Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cell Biology 2021Quote: ... were transfected into 293TN cells (System Biosciences) using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293TN cells were purchased from System Biosciences. The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... and HEK293TN cells (System Biosciences, #LV900A-1) were routinely cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293TN cells were obtained from System Biosciences and cultured in DMEM supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Colonies were stained using an Alkaline Phosphatase Assay Kit (System Biosciences) and evaluated under an Olympus CK2 microscope.
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bioRxiv - Neuroscience 2021Quote: ... Exosomes were extracted from supernatant with an ExoQuick kit (System Biosciences). The presence of exosomal markers and size distribution of the particles was confirmed previously [22] ...
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bioRxiv - Neuroscience 2020Quote: ... fibrinogen and fibrin proteins known to impede efficient EV recovery were removed using 5 U/mL of thrombin (System Biosciences, Inc.; Mountainview, CA) for 30 minutes at RT followed by addition of 495 μL of Dulbecco’s PBS-1X (DPBS ...
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bioRxiv - Molecular Biology 2023Quote: ... a minimal piggyBac cargo construct was created by deleting all intervening sequences between the 5’ and 3’ terminal repeats (including core insulators) of the PB-CMV-MCS-EF1α-Puro vector (System Biosciences, PB510B-1). A gBlock (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293TN cells (System Biosciences, cat. no. LV900A-1) and HeLa cells (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH ...
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bioRxiv - Biochemistry 2021Quote: ... or ExoQuick ULTRA EV Isolation Kit for Serum and Plasma (System Biosciences), and protocols were performed according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Microvesicles were isolated using ExoQuick-TC kit (System Biosciences, Palo Alto, USA) accordingly to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... samples were purified using the CD9 Exo-Flow Capture Kit (System Biosciences) using the manufacturer-provided protocol ...
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bioRxiv - Bioengineering 2022Quote: Gene editing of COS-7 cells were performed by electroporation of COS-7 cells with Super PiggyBac plasmid (PB210PA-1, System Biosciences) and one of the 5 plasmids of pBv1-EF-6X ...
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bioRxiv - Cancer Biology 2019Quote: Neuroblastoma cell lines were obtained from the Children’s Hospital of Philadelphia (CHOP) neuroblastoma cell line bank and 293T cells from System Biosciences (LV900A-1); all were cultured as previously described ...
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bioRxiv - Molecular Biology 2019Quote: HeLa cervix carcinoma cells (American Tissue Culture Collection: Manassas, VA, USA) and human embryonic kidney (HEK) 293TN cells (System Biosciences, LV900A-1) were cultured using Dulbecco’ s Modified Eagle Medium (DMEM ...
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bioRxiv - Developmental Biology 2022Quote: AFSC-EVs were fluorescently labelled using ExoGlow-EV labeling kit (SBI System Biosciences), using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... concentrated by PEG-it™ precipitation kits from System Bioscience (Palo Alto, CA), were re-suspended in cold PBS and stored at −80 °C.
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bioRxiv - Developmental Biology 2022Quote: ... pre-digested with XhoI and HindIII using Cold Fusion Cloning kit (System Biosciences). Deletion of putative TF binding sites from those enhancers were performed using QuickChange II XL site-directed mutagenesis kit (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2024Quote: ... we utilized the MC-Easy™ Minicircle DNA Production Kit (System Bioscience, SBI). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... EVs were quantified with the ExoELISA Complete Kit (CD63 Detection, System Biosciences, USA) according to manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: 293TN Producer cell line (System Biosciences, Cat #LV900A-1) was maintained in DMEM containing 10% FBS ...