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Citations for Mirus Bio :
451 - 500 of 529 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... cells were seeded into six-well plates (Orange Scientific) at a density of 300.000 cells/well and transfected the next day with Trans-IT LT1 (Mirus, WI, USA) and 1:1:1:1 μg DNA encoding hASIC1a TAG variants ...
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bioRxiv - Microbiology 2021Quote: ... Maxiprep purified plasmids were transfected into HEK293FT cells using PEI or into ACH2 / U1 leukocytes via TransIT-Jurkat reagent (Mirus #2120). Supernatants were collected 72 hours after media replacement (PEI ...
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bioRxiv - Microbiology 2020Quote: Viral particles were produced by transfection of 293FT cells in 6-well plates with 3 μg DNA and 8 μl TransIT-293 Transfection Reagent (Mirus Bio) per well ...
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bioRxiv - Cell Biology 2021Quote: ... C-terminal GFP-2x Strep-tagged full length and truncation versions of CEP90 were cloned into pLVX-EF1α-IRES-Puro backbone and transfected into 293T cells using TransIT-293 transfection reagent (Mirus Bio) according to manufacturer guidelines.
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bioRxiv - Microbiology 2020Quote: ... Confluent monolayers of BHK-T7 cells in 12-well plates were co-transfected using TransIT-LT1 transfection reagent (Mirus Bio LLC) with 0.27 μg of each of eleven plasmid constructs representing the SA11 rotavirus genome and of each of two plasmids encoding vaccinia virus mRNA capping enzymes ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.2 μg pVSVG per 1 reaction in a 6-well plate well) into HEK 293T cells with TransIT-LT1 transfection reagent (Mirus #2304) following manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Lentiviral vector was produced by transforming the produced LARRY plasmid into HEK293T cells using the Trans-IT 293 transfection reagent (Mirus #MIR2700). For each of eighteen 10 cm dishes of HEK293T cells ...
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bioRxiv - Cell Biology 2022Quote: ... carrying the sequence of the gene to be over expressed and resistance to puromycin into 0.8×106 HEK293 cells in a 60 mm culture dish with 6 µl of TrasnIT (Cat. Nº: Mirus Bio. 293) containing 3 ml of complete media ...
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bioRxiv - Microbiology 2022Quote: ... and were transfected into early passage NIH-3T3 cells in 6 well plates using TransIT-X2® dynamic delivery transfection system (Mirus). Viruses from cell culture supernatants were passaged on M2-10B4 cells followed by virus purification and titration [3] ...
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bioRxiv - Neuroscience 2022Quote: ... and Fc (a negative control) were generated using HEK293T cells transfected with the corresponding expression vectors using TransIT-PRO Transfection Reagent (Cat# MIR5740, Mirus Bio) and maintained in serum-free AIM V synthetic medium (Cat# 12055083 ...
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bioRxiv - Synthetic Biology 2023Quote: Cells were seeded in 96-well plates one day before transfection with TransIT-PRO transfection kit (Mirus Bio, Madison, WI, US) according to manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2023Quote: ... Neuro2a cells were seeded in 24-well plates at 80,000 cells per well and on the next day were transfected with TransIT-LT1 Transfection Reagent (Mirus, MIR 2300), using 150 ng luciferase reporter ...
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bioRxiv - Cell Biology 2022Quote: HeLa cells were transiently transfected with pLVX-EF1alpha-nCoV2019 2xStrep- tagged SARS-CoV2 ORF constructs [47] using TransIT-HeLa Monster (Mirus Bio). After 48 hours transfection ...
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bioRxiv - Cell Biology 2022Quote: ... 7 and 10 in this background were obtained by transfecting the adherent parental cell line with PX459 encoding the respective sgRNA using TransIT-293 transfection reagent (Mirus, USA). 48 h post transfection ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were grown on coverslips and transiently transfected with mCherry Parkin and PPP3CB-Flag using Trans-IT transfection reagent (Mirus Bio) for 18 h ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Baculoviruses were produced by transfecting Sf9 cells with recombinant bacmids using TransIT®-Insect Transfection Reagent (Mirus, cat no. MIR 6100), grown for 7 days at 28ºC ...
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bioRxiv - Microbiology 2023Quote: ... MRC-5 cell monolayers were mock-transfected or transfected with sHCoV genomic RNA (1 μg/ml) using TransIT-mRNA Transfection Kit (Mirus Bio) at 33°C ...
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bioRxiv - Immunology 2023Quote: ... were seeded in 15-cm2 dishes at a density of 10 × 106 cells per dish and the following day were transfected with 25 µg of spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were transfected with pCAG-Cas9-EGFP (gift from Randy Platt) and sgRNAs plasmids with TransIT-LT1 Transfection Reagent (Mirus, MIR2305) and after 48 h were sorted with BD FACSAria III Cell Sorter (BD FACSDiva Software ...
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bioRxiv - Molecular Biology 2023Quote: ... the generated vectors and the PIK packaging plasmid were delivered into HEK 293T cells using the TransIT-LT1 transfection reagent (Mirus Bio). Stable MEF cell lines expressing either wild-type (WT ...
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bioRxiv - Cell Biology 2023Quote: ... and pMDLg/pRRE) at a 1:1 ratio and transfected into HEK293T cells using TransIT LT1 transfection reagent (Mirus Bio LLC). After 72 h virus-containing supernatants were collected ...
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bioRxiv - Microbiology 2023Quote: RBP-overexpressing Huh7 cells were generated by transfection of RBP expression plasmids using TransIT LT-1 transfection reagent (Mirus, WI, USA). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... pCW57.1-HLTF vectors were packaged into lentivirus particles using the 2nd generation lentiviral packaging system (pMD2.G & psPAX2) in HEK293T cells using TransIT®-LT1 Transfection Reagent (Mirus). Virus-containing media was harvested 24 & 48h post-transfection and filtered through 0.45 μm PES membrane syringe filter to eliminate packaging cells ...
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bioRxiv - Cell Biology 2023Quote: ... CCDC47 and TMEM147 were obtained by transfecting HEK 293T cells with pX459 encoding the respective sgRNA using TransIT-293 transfection reagent (Mirus, USA). 72 h post transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... At 30% confluence cell were transfected with siRNAs at final concentrations of 20 nM using transfection agent TransIT-X2® Dynamic Delivery System (Mirus). Cells were lysed for western blot and/or fixed for confocal microscopy analysis and images acquisition ...
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bioRxiv - Biophysics 2024Quote: ... Lentiviral particles containing each plasmid were produced by transfecting standard packaging vectors along with the plasmids into HEK293T cells using TransIT-LT1 Transfection Reagent (Mirus, MIR2306). Media was changed at 24 hours post-transfection ...
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bioRxiv - Cell Biology 2019Quote: ... plasmid vectors coding the glycoproteins were transfected into BSR-T7 cells using the TransIT®-LT1 Transfection Reagent (Mirus Bio, MIR 2300). At 16 h post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... and RdRp-N462 or RdRp-D462 were transfected into 293T cells at a 1:1:1 ratio using TransIT LT-1 (Mirus, Madison, WI). To normalize transfection efficiency ...
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bioRxiv - Microbiology 2019Quote: ... were plated at 2 × 105 cells/mL in 2 mL in 6-well plates one day prior to transfection using TransIT-LT1 reagent (Mirus Bio LLC) with 3 μL of transfection reagent per μg of DNA ...
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bioRxiv - Genomics 2020Quote: ... cells had reached 80% confluence and were transfected with plasmids for lentivirus production using TransIT-LT1 transfection reagent (Mirus Bio, Madison, WI). 3 μl transfection reagent was added to a total volume of 15 μL Opti-MEM (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... and replication-competent HIV-1 were produced by transfection into 293T cells with the TransIT-Lenti transfection reagent (Mirus Bio, Madison, WI) at a ratio of 1:3 (DNA ...
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bioRxiv - Immunology 2019Quote: Lentivirus was produced by transfecting lentiviral plasmids and 2nd generation packaging/polymerase plasmids into 293T cells using TransIT-LT1 Reagent (Mirus Bio LLC). Virus-containing supernatants were harvested 72h later ...
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bioRxiv - Genetics 2019Quote: ... Y15F and Y15F mutant cDNA were generated as described above and transfected into HEK-293T cells (ATCC; CRL-3216) using TransIT®-LT1 Transfection Reagent (Mirus; MIR2305) following user instructions ...
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bioRxiv - Immunology 2021Quote: ... the RP172 plasmids containing the CHST genes were co-transfected with RP18 and RP19 helper plasmids into HEK293T cells after incubating with TransIT®-LT1 transfection reagent (Mirus Bio). After 3 days ...
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bioRxiv - Microbiology 2020Quote: ... A 1:1:1 molar ratio of all three plasmids for a total DNA concentration of 20 μg or 40 μg (T75 vs. T150 flask) was used to transfect the cells using the TransIT®-LT1 Transfection Reagent (Mirus Bio). Three days later ...
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Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 ComplexbioRxiv - Cell Biology 2020Quote: ... HeLa cells stably expressing GFP-FYVE(SPG15) were created using retrovirus made in HEK 293ET cells transfected using TransIT-293 Transfection Reagent (Mirus Bio LLC), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Transient transfection experiments were performed in six-well plates at 2.5 × 105 HEK 293 T cells per well using TransIT®-LT1 transfection reagent (Mirus Bio LLC) according to the manufacturer’s instructions unless indicated.
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bioRxiv - Cell Biology 2022Quote: ... constructs were packaged into lentivirus in a 10 cm plate 60% confluent of HEK293T cells using the TransIT-LT1 (Mirus Bio, MIR2300) transfection reagent and 7 μg of the vector ...
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bioRxiv - Microbiology 2022Quote: ... cells were transfected with 1 µg of DDX60 wt or mutant constructs using TransIT®-LT1 Transfection Reagent (Mirus Bio MIR 2300) at a 1:3 (µg to µl ...
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bioRxiv - Cell Biology 2019Quote: ... and a circular Rpl13a repair template into cultured Drosophila S2 cells using TransIT Insect Transfection Reagent (Mirus Bio LLC, Madison, WI, USA). Gene editing efficiency of the different sgRNAs was evaluated five days after transfection by cellular fluorescence and genotyping using a primer outside of the homology arm and the other inside the inserted PQR sequence.
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bioRxiv - Cell Biology 2019Quote: ... or a GFP expression vector with packaging vectors (CMVΔR8.91, CMV-VSV-G) into 293T cells using Mirus TransLTi (Mirus Bio LLC, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... were used to transfect 1 × 106 Huh-7 cells using the TransIT® mRNA transfection kit according to the manufacturer’s instructions (Mirus Bio LLC). At 72 h post transfection (p.t.) ...
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bioRxiv - Genetics 2020Quote: ... shUBR5 and shScrambled-pLKO.1-puro were co-transfected with pCMV-VSV-G and pCMV-dR8.2 dvpr plasmids into HEK 293T cells using TransIT293 reagent (Mirus Bio LLC, USA). To generate stable silenced shUBR5 cells ...
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bioRxiv - Genetics 2021Quote: Plasmid expressing HA-tagged human WFS1 was transfected into HeLa cells using TransIT-X2® Dynamic Delivery System (Mirus Bio; MIR 6000). After 24 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were passaged to 12-well tissue culture plates at a density of 1.5×105 cells/well (3.9×104 cells/cm2) and transfected with 500 ng plasmid DNA using 2 uL of TransIT-X2 transfection reagent (Mirus Bio, #MIR6005) and 100 uL Opti-MEM I reduced serum medium (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Monolayers of BHK-T7 cells at ~50% confluency in 6-well plates were co-transfected using TransIT-LT1 transfection reagent (Mirus Bio LLC) with 0.8 μg of each of ten plasmid constructs representing the T1L or T3DI reovirus genome ...
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bioRxiv - Cell Biology 2021Quote: Lentivirus is produced in HEK293t cells by transfection of lentiviral plasmid and packaging plasmids pMD2.G and psPAX2 using TransIT-VirusGEN® Transfection Reagent (Mirus, MIR6705). After two days of transfection ...
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bioRxiv - Microbiology 2022Quote: 293T cells were seeded at 2 × 105 cells/well in 6-well plates the day before transfection using TransIT-LT1 reagent (Mirus Bio LLC) at 3 μL transfection reagent/well as previously described 57 ...
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bioRxiv - Microbiology 2022Quote: HEK293T were seeded at 2 × 105 cells/well in 6-well plates the day before transfection using TransIT-LT1 reagent (Mirus Bio LLC) at 5.8 μL transfection reagent/well ...
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bioRxiv - Molecular Biology 2022Quote: ... Lentivirus was produced by transfecting HEK293T cells with standard packaging vectors using the TransIT-LT1 Transfection Reagent (MIR 2306; Mirus Bio LLC). Viral supernatant was collected 48–72 h after transfection ...