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Citations for Mirus Bio :
1 - 50 of 529 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Clonal cell lines expressing GFP/biotin ligase fusions were generated using TransIT transfection reagent (Mirus) by co-transfection of 0.6µg/ml expression plasmid with 0.06µg/ml pCoBLAST selection plasmid (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells containing RNP and donor DNA were electroporated at 150V using the Ingenio EZporator Electroporation System (Mirus). Cells were incubated at ambient temperature for 5 min and transferred to a six-well plate containing 2 mL growth medium with or without combinations of 1 μM AZD7648 ...
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bioRxiv - Biochemistry 2023Quote: ... MCF10A cells were treated with an additional 750 μL of medium containing 3 μM puromycin (Mirus Bio 5940), achieving a final concentration of 1 μM (0.5 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... CHO-S cells were transfected with VHH-Fc containing construct using the CHO Gro System (Mirus Bio, WI, USA), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Sanger sequencing-verified pX330 plasmids containing the correct sequences of gRNAs were transfected into KB1P-G3 cells using the TransIT-LT1 transfection reagent (Mirus) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: WT cells were transfected with the sgRNA-containing plasmids by electroporation with a Nucleofector device (Lonza; in Mirus transfection solution). Cells were plated in culture dishes and maintained for three days ...
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bioRxiv - Microbiology 2022Quote: ... 6-well plates seeded with HEK293T cells were transfected with the m145 containing expression plasmids using TransIT-X2® dynamic delivery transfection system (Mirus) and cells were harvested at 48 hours’ post transfection ...
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bioRxiv - Cell Biology 2021Quote: Biotin was covalently coupled to pDNA (pGL3 plasmid) by using Nucleic Acid Labeling Kit according to manufacturers’ protocol (Label IT® Nucleic Acid Labeling Kit, Biotin, Mirus Bio, USA). Briefly ...
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bioRxiv - Systems Biology 2020Quote: ... Following 1 week of incubation with a selection media containing G418 (Mirus Bio LLC) at 1 mg/mL ...
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bioRxiv - Biophysics 2020Quote: ... Following 1 week of incubation with a selection media containing G418 (Mirus Bio LLC) at 1mg/mL ...
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bioRxiv - Developmental Biology 2019Quote: ... containing the three lentiviral plasmids and 60 μl of TransIT Reagent (Mirus Bio, 2700) diluted in 1.5 ml of Opti-MEM (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... Strains containing selectable markers were cultured on medium with 50 µg/mL hygromycin (Hygromycin B Solution, Mirus) or 25 µg/mL kanamycin (GoldBio ...
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bioRxiv - Cell Biology 2020Quote: ... cells were treated with the chemicals for 30 min before a transfection mixture containing 0.2 μg mCherry-expressing plasmid and 0.6 μl TransIT293 (Mirus) was added to the cells ...
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bioRxiv - Microbiology 2020Quote: ... a 10x mix was prepared in OptiMEM containing siRNA(s) and TransIT-X2 transfection reagent (MIR 600x, Mirus) in a 1:2 stoichiometry ...
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bioRxiv - Microbiology 2023Quote: ... and 5 μg of pSBbi or pSBtet containing gene of interest using TransIT-LT1 transfection reagent (Mirus Bio), following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... and 1.5 μg of the plasmid containing the gene of interest were cotransfected by using TransIT transfection reagent (Mirus) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... cultured in Schneider’s Drosophila Medium containing 10% heat-inactivated FBS and 1X penicillin-streptomycin with the TransIT-LT 1 reagent (Mirus). Flag-CG30403 only (no bait ...
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bioRxiv - Genetics 2020Quote: ... Constructs containing FP-GRIP1-KBD +/- exon 21 and FLAG-BicD2-KIF5A were expressed by transfection using TransIT-LT1 (Mirus). Approximately 24 hours after transfection ...
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bioRxiv - Microbiology 2021Quote: ... a 10x mix was prepared in OptiMEM containing the appropriate siRNA(s) and TransIT-X2 transfection reagent (MIR 600x, Mirus) in a 1:2 stoichiometry ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected using Grem1 sgRNA-containing plasmid or the cloning backbone (empty vector) using the TransIT-X2 Dynamic Delivery System (Mirus). After 24 hrs ...
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bioRxiv - Bioengineering 2023Quote: ... low passage cells were plated at a density of 25,000 cells per well in a 96-well plate and transfected at > 80% confluence with all-in-one PiggyBac plasmids containing CasRx using TransIT-X2 (MIR 6003, Mirus) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were transfected with 10 ug of the TRE library ± 5 ug of pcDNA3.1 containing a GPCR expression cassette using TransIT-2020 (Mirus Bio) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 1mM NEAA and penicillin–streptomycin) were transfected with 500μl Opti-MEM containing 30 μl TransITβ-LT1 Transfection Reagent (Mirus, MIR2305), 10μg pLV-tetO-Tfap2c ...
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bioRxiv - Microbiology 2023Quote: ... Medium was changed 24 h later to 10 ml of 5 % FBS/DMEM and changed again 48 h later to 10 ml of 5 % FBS/DMEM containing 1 µg/ml of puromycin (Mirus). Cells were incubated in selection medium for 72 h ...
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bioRxiv - Cell Biology 2020Quote: ... a plasmid containing a cAMP response element binding site driving firefly luciferase was transfected with a control plasmid containing a CMV promotor driving renilla luciferase expression using the TransiT-X2 transfection reagent (Mirus Bio) 48 hours after the siRNA transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then transfected with a Cas9-GFP-expressing plasmid (pSpCas9(BB)-2A-GFP) and the gRNA containing STAgR-Neo plasmid using the TransIT-X2 transfection reagent (Mirus Bio LLC) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and a full-length plasmid containing the chimeric LLOV genome (1 μg) using TransIT®-LT1 per manufacturer’s recommendations (Mirus Bio LLC). Media was changed approximately 18 hours post-transfection and cells were monitored for cytopathic effect (CPE ...
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bioRxiv - Molecular Biology 2021Quote: ... was transfected into naïve cells or METTL18 KO cells by TransIT-293 (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ... Ad293 cells were cultured in 96 well plates with DMEM containing 10% FB Essence (Avantor Seradigm, USA) and transfected using Transit-LT1 (Mirus Bio, Madison, WI, USA) with plasmid encoding 4 LXR response elements fused with luciferase ...
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bioRxiv - Biochemistry 2024Quote: ... they were transfected with 1800 ng of plasmid containing either N- or C-terminal tagged GFP-AMPKγ3 using TransITx2 transfection reagent (Mirus Bio, cat no. MIR 6000) and then lysed 48 hours later (lysis buffer – 50 mM Tris ...
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bioRxiv - Genomics 2020Quote: ... HEK293T cells (4 × 105 cells/ 6-well dish) were transfected using LT1 reagent (Mirus Bio) with HDV-EGFP (1 ug) ...
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bioRxiv - Immunology 2020Quote: HeLa cells were transfected using TransIT-HeLaMONSTER and HEK293 cells using TransIT-293 (both from Mirus). Cells after transfection were cultured both with and without ProS or HS in the medium ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells with HeLa monster (Mirus), and NIH3T3 cells with Trans-IT X2 (Mirus) ...
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bioRxiv - Microbiology 2022Quote: ... cells were transfected (TransIT-mRNA, Mirus) with 5µg of replicon RNA previously obtained by in vitro transcription ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Cell-free viruses were produced by transfection of HEK293 cells with pNL4-3 using TransIT-LT1 (Mirus) or Fugene HD (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... using jetOPTIMUS (U251 cells; Polyplus-transfection, Illkirch, France) or TransIT-LT1 (A549 cells; Mirus Bio, Madison, WI) according to the protocols provided by the manufacturers ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 10 µg of DNA at 70-80% cell density using TransIT-LT1 (Mirus, 2300) for 293T cells and at 50-60% cell density with Lipofectamine 3000 (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... whereas MEF cells used TransIT-X (Mirus) following manufacturer protocol with varied DNA concentrations ...
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bioRxiv - Molecular Biology 2023Quote: ... for HeLa cells or TransIT-293 (Mirus) for HEK T-REx cells following manufacturers’ protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... Caco-2 cells were plated (10·106 cells) in 500μl of the Ingenio electroporation solution (Mirus Vio, Wisconsin, USA). The transfected cells were selected using the antibiotic geneticin/G418 (Gibco-Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: 293T cells were seeded in 6-well plates at 0.2M cells/ml and were transfected with TransIT-LT1 (Mirus) 24h later with 1500 ng of empty plasmid ...
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bioRxiv - Cell Biology 2024Quote: Transfection of Capsaspora cells and stable cell line generation was done using the TransIT-X2 transfection reagent (Mirus Bio) as previously described (Phillips et al. ...
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bioRxiv - Microbiology 2020Quote: The CPER products (25 μl out of a 50 μl reaction volume) were transfected into HEK293-3P6C33 cells or BHK-21 cells with Trans IT LT-1 (Mirus), following the manufacturer’s protocols ...
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bioRxiv - Immunology 2024Quote: ... viral RNA isolated from infected BHK cells was transfected into cells at a concentration of 500 µg/ml using TransIT-mRNA transfection kit (Mirus). RABVLe RNA (2 pmol/ml ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293 cells were transfected with TransIT-293 (Mirus) according to manufacturer’s instructions with three plasmids ...
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bioRxiv - Cell Biology 2020Quote: HEK293T cells were transfected using TransIT-293 (Mirus) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: HEK293T cells were transfected using TransIT-X2 (Mirus) reagent ...
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bioRxiv - Cell Biology 2021Quote: ... and NIH3T3 cells with Trans-IT X2 (Mirus), according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... 293T cells were transfected using Trans-IT (Mirus) with a plasmid carrying the lentiviral backbone (pHAGE-EF1αL-GFP ...
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bioRxiv - Molecular Biology 2022Quote: ... TamS cells were transduced using a lentivirus (Mirus Trans-Lenti Transfection Reagent ...