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Citations for Mirus Bio :
401 - 450 of 784 citations for RPA kit reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... or 2:2:1 (33) with TransIT®-LT1 transfection reagent (Mirus Bio LLC, Madison, WI, USA) and the plasmids ...
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bioRxiv - Microbiology 2023Quote: ... All transfections used 2.49 µg plasmid DNA with 6.25 µL TransIT LT1 transfection reagent (Mirus, Madison, WI) in 250 µL Opti-MEM (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: ... pcDNA3.1 Flag-MAVS-C79F or pcDNA3.1-Flag-MAVS-CcardA expressing constructs using TransIT-LT1 transfection reagent (Mirus) before selection using 800µg/ml geneticin (G418 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... pLAS2w.Fluc.Ppuro and pcDNA3.1-SCoV2-S(B.1.1.7) related spike expression constructs using TransITR-LT1 transfection reagent (Mirus). Culture media were refreshed at 16 hr and harvested at 48 hr and 72hr post-transfection ...
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bioRxiv - Genetics 2023Quote: ... Transfections of wild-type and Gly1170Ser COL2A1-encoding plasmids were performed using Transit-2020 transfection reagent (Mirus) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP- and FLAG-tagged proteins under the control of the Actin promoter using TransIT-LT1 reagent (Mirus). 24-40 h after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293T cells were transfected at 60-90% confluence with TransIT®-LT1 Transfection Reagent (#2300, Mirus Bio) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Each dish was transfected using the TransIT-LT1 Transfection reagent (Mirus, MIR 2305, 3µL/µg of DNA) with 4.8μg psPAX2 ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293T cell line was used for retroviral and lentiviral system amplification with TransIT-293 Transfection Reagent (Mirus).
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bioRxiv - Microbiology 2023Quote: ... and 1 µg PMD2.G packaging plasmids using 25 µL TransIT-LT1 Transfection Reagent (Mirus, MIR 2306). After 72 h of transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... The following day (Day 0) plated cells were transfected with the TransIT-LT1 Transfection Reagent (Mirus #2306), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... pSPAX2 and Nb80-GFP and SNAP-β1AR plasmids using as transfection reagent TransIT-Lenti (mir6600, Mirus bio). Lentivirus was produced in DMEM containing 10% FBS and 1% BSA and then concentrated using the Lenti-X concentrator (Takera Bio).
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bioRxiv - Microbiology 2023Quote: HEK293 were transfected with pMTBS and pCS2-ST at ~80% confluence using TransIT-293 transfection reagent (Mirus). Cells were harvested for downstream applications 48 hours post-transfection.
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bioRxiv - Immunology 2023Quote: ... These DNA mixes were incubated with 18 mL OptiMEM and 1 mL of TransIT-LT1 reagent (Mirus) for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... with a 4:2:1:1 ratio of the target:pMDLg:pMD2.G:pRSV-REV plasmids using Transit 293T reagent (Mirus). After 48 hours ...
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bioRxiv - Microbiology 2020Quote: ... 2.5µg of pCAGGS NA-FLAG (either NAg+ or NAg-) were transfected using 7.5µl of LT1-TransIT transfection reagent (Mirus). Twenty-four hours post transfection ...
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bioRxiv - Cell Biology 2020Quote: ... transfected at confluence (Day -2) using Mirus TransIT-X2 transfection reagent (3ul per well; Mirus Bio LLC, US) and 250 ng plasmid DNA (either empty vector or FABP4 sgRNA) ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant baculoviruses were recovered by transfection of the bacmids into Sf9 insect cells using TransIt-Insect reagent (Mirus). Viruses were then used to infect suspension High Five cells grown in Express5 media and supernatants harvested ∼60–72 hours post-infection ...
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bioRxiv - Genetics 2021Quote: ... plv-sgDiR-EGFP with different guide sequences) were transfected into 10 million 293T using TransIT-LT1 reagent (Mirus), lentivector ...
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bioRxiv - Cancer Biology 2020Quote: ... 7.5ug PsPax2 and 2.5ug VSV-G using TransIT-LT1 transfection reagent via manufacturer instructions (Mirus; Cat#: MIR 2305). FUGW (Addgene ...
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bioRxiv - Microbiology 2021Quote: ... All transfections were performed in OptiMEM (LifeTechnologies) using Lipofectamine LTX + PLUS reagent (LifeTechnologies; 15338100) or TransIT-LT1 (Mirus) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: Cells were plated at ~20-50% confluence and transfected the following day using TransIT-293 transfection reagent (Mirus) and OPTI-MEM (1105802 ...
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bioRxiv - Immunology 2022Quote: ... The plasmid cocktail was then gently mixed with 15 μL of TransIT LT1 transfection reagent (Mirus, GA, USA). The growth media was replaced with Opti-MEM during transfection ...
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bioRxiv - Microbiology 2022Quote: All transfections in this study were performed with TransIT-X2® Transfection Reagent (Mirus Bio, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Phoenix Ecotropic (pECO) cells (ATCC CRL-3214) were transfected with plasmids using TransIT-293 transfection reagent (MIR2705, Mirus). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... a 10x mix was prepared in OptiMEM containing siRNA(s) and TransIT-X2 transfection reagent (MIR 600x, Mirus) in a 1:2 stoichiometry ...
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bioRxiv - Cancer Biology 2019Quote: ... Using either the DharmaFECT 1 Transfection Reagent (T-2001-02, Dharmacon) or the Mirus TransIT-X2® (Mirus) system as per the manufacturers’ instructions ...
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bioRxiv - Microbiology 2020Quote: ... Monolayers of ∼ 8 × 105 BHK-T7 cells were co-transfected using TransIT-LT1 transfection reagent (Mirus Bio LLC) with 0.8 μg each of nine plasmid constructs representing the T1L reovirus genome plus a single parental or mutant pBacT7-S1 plasmid ...
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bioRxiv - Immunology 2021Quote: ... A transfection mixture was prepared 1ml OptiMEM that contained 30 µl of TransIT-Lenti transfection reagent (Mirus MIR6600) and 6 µg pALD-Fluc ...
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bioRxiv - Cell Biology 2022Quote: ... 10g of plasmid DNA was transfected into 293T lentiviral packaging cells using LT1 transfection reagent (Mirus Bio LLC), and viral supernatants were collected ...
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bioRxiv - Cell Biology 2022Quote: ... Lentivirus was made by transfecting Lx293T with standard packaging vectors and TransIT-LT1 Transfection Reagent (Mirus, MIR 2306). Viral supernatant was harvested two days after transfection ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were transfected with 7.5 µg (total amount) of indicated plasmid(s) using TransIT-2020 transfection reagent (per the manufacturer’s recommendation, Mirus). Following 24–36 h incubation period ...
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bioRxiv - Neuroscience 2022Quote: ... cells were transfected with 2 μg of DNA 24 - 48 hours before measurement using TransIT transfection reagents (Mirus). DRG neurons from adult (postnatal weeks 8–12 ...
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bioRxiv - Biochemistry 2023Quote: ... transfected with 9 μg HA-tagged and 9 μg Flag-tagged constructs using TransIT-LT1 transfection reagents (Mirus). The transfected cells were cultured for another two days ...
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bioRxiv - Molecular Biology 2023Quote: ... A transfection mix was prepared by adding 2.5 µg plasmid and 7.5 µl transfection reagent TransIT-LT1 (Mirus) in 250 µl OptiMEM medium ...
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bioRxiv - Cell Biology 2022Quote: ... Candidate and control vectors were tested for activity by transient transfection with Trans-IT LT1 transfection reagent (Mirus) into murine neuron-like N2A cells followed by Trizol RNA isolation (Thermo ...
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bioRxiv - Cell Biology 2024Quote: ... pPBhCMV1-Prdm14-pA and pPBhCMV1-Nanog-pA vectors were transfected using TransITβ-LT1 Transfection Reagent (Mirus BioTM, MIR2304) into ESCs together with pPBCAG-rtTA and pPyCAG-PBase vectors ...
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bioRxiv - Microbiology 2023Quote: ... and 5 μg of pSBbi or pSBtet containing gene of interest using TransIT-LT1 transfection reagent (Mirus Bio), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... using TransIT reagent (0.25 µl/well, 96-well plate, and 1 µl/well, 48-well plate, Mirus Bio). The transfection mix was replaced with fresh medium after 4 hours ...
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bioRxiv - Genetics 2023Quote: ... lentiviral particles were produced by transfecting standard packaging vectors into HEK293T using TransIT-LT1 Transfection Reagent (Mirus, MIR2306). At <24 hours post-transfection culture media with exchanged with fresh media supplemented with ViralBoost (Alstem Bio ...
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bioRxiv - Cell Biology 2024Quote: ... with 10% FBS) were transfected using TransIT-X2 or TransIT-LT1 Transfection Reagent (Mirus; using the manufacturer’s protocol) with 250 ng pCMV-VSG ...
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bioRxiv - Cell Biology 2020Quote: ... together with the packaging plasmids psPAX2 (UK1701) and pMD2.G (UK1700) at a 10:7.5:5 ratio using TransIT-293 reagent (Mirus). The supernatant containing the viral particles was collected 48 hrs after transfection ...
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bioRxiv - Immunology 2021Quote: HEK 293T cells (106) were transfected in 6-well plates with corresponding plasmids with Transit LT1 transfection reagent (Mirus) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 μg PMD2.G packaging plasmids using 25 μl TransIT®-LT1 Transfection Reagent (Cat# MIR 2306, Mirus). After 72 h of transfection ...
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bioRxiv - Biophysics 2022Quote: ... and 1.5 μg of the plasmid containing the gene of interest were cotransfected by using TransIT transfection reagent (Mirus) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... HEK293 cells were transfected with either 2.5 or 8 ug of the pCMV-Aire-Flag plasmid (or control plasmid) using 7.5 or 32 ul of the TransIT-293 transfection reagent (Mirus). After 48h ...
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bioRxiv - Cell Biology 2019Quote: ... and 1.2 μg cytomegalovirus 8.91 vector were mixed and prepared for transfection using TransIT-293 Transfection Reagent (Mirus Bio) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Transfections were performed on 150 mm dishes (8 per condition) using Mirus TransIT®-LT1 Transfection Reagent (Mirus Bio) and Lipofectamine 2000 (Life Technologies) ...
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bioRxiv - Genomics 2019Quote: ... 4 to 8-week-old mice were injected intravenously with 1 μg of mRNA formulated with TransIT transfection reagent following the manufacturer’s suggestions (Mirus). The procedure for one mouse was as follows ...
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bioRxiv - Cancer Biology 2019Quote: ... in combination with 3μg psPAX2 (packaging plasmid) and 2μg pMD2.G (envelope plasmid) with 30μL TransIT-LT1 transfection reagent (Mirus, MIR 2300). For T-ALL cell infection ...